Brilliant Violet 421™ anti-human CD178 (FasL) | BioLegend 306411 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

Brilliant Violet 421™ anti-human CD178 (FasL)

catalog :

306411

quantity :

25 tests

price :

182 USD

clonality :

monoclonal

host :

mouse

conjugate :

BV421

clone name :

NOK-1

reactivity :

human

application :

flow cytometry

more info or order :

BioLegend product webpage

citations: 10

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; 1:200; fig s1-2j	Jeong D, Kim H, Kim H, Kang M, Jung H, Oh Y, et al. Soluble Fas ligand drives autoantibody-induced arthritis by binding to DR5/TRAIL-R2. elife. 2021;10: pubmed publisher
	Guo X, Kazanova A, Thurmond S, Saragovi H, Rudd C. Effective chimeric antigen receptor T cells against SARS-CoV-2. iScience. 2021;24:103295 pubmed publisher
	Fenton T, Jørgensen P, Niss K, Rubin S, Mörbe U, Riis L, et al. Immune Profiling of Human Gut-Associated Lymphoid Tissue Identifies a Role for Isolated Lymphoid Follicles in Priming of Region-Specific Immunity. Immunity. 2020;52:557-570.e6 pubmed publisher
	Mooren F, Völker K, Klocke R, Nikol S, Waltenberger J, Krüger K. Exercise delays neutrophil apoptosis by a G-CSF-dependent mechanism. J Appl Physiol (1985). 2012;113:1082-90 pubmed publisher
	Shrestha B, Pinto A, Green S, Bosch I, Diamond M. CD8+ T cells use TRAIL to restrict West Nile virus pathogenesis by controlling infection in neurons. J Virol. 2012;86:8937-48 pubmed publisher
	Qin G, Liu Y, Zheng J, Xiang Z, Ng I, Malik Peiris J, et al. Phenotypic and functional characterization of human ?? T-cell subsets in response to influenza A viruses. J Infect Dis. 2012;205:1646-53 pubmed publisher
	Khalid M, Yu H, Sauter D, Usmani S, Schmökel J, Feldman J, et al. Efficient Nef-mediated downmodulation of TCR-CD3 and CD28 is associated with high CD4+ T cell counts in viremic HIV-2 infection. J Virol. 2012;86:4906-20 pubmed publisher
	Kruger K, Agnischock S, Lechtermann A, Tiwari S, Mishra M, Pilat C, et al. Intensive resistance exercise induces lymphocyte apoptosis via cortisol and glucocorticoid receptor-dependent pathways. J Appl Physiol (1985). 2011;110:1226-32 pubmed publisher
	Li J, D Alessio A, Pober J. Lipopolysaccharide can trigger a cathepsin B-dependent programmed death response in human endothelial cells. Am J Pathol. 2009;175:1124-35 pubmed publisher
	Strauss L, Bergmann C, Whiteside T. Human circulating CD4+CD25highFoxp3+ regulatory T cells kill autologous CD8+ but not CD4+ responder cells by Fas-mediated apoptosis. J Immunol. 2009;182:1469-80 pubmed

product information

Antigen :

CD178

Apps. Abbrev. :

Cat # :

306411

Clone :

NOK-1

Item :

Brilliant Violet 421™ anti-human CD178 (FasL)

Isotype :

Mouse IgG1, κ

Other Names :

Fas Ligand (FasL), CD95L, TNFSF6, Fas-L

Size :

25 tests

Price (USD) :

182 USD

Reactivity :

Human

Clonality :

Monoclonal

Host :

Mouse

Conjugate/Tag/Label :

BV421

Immunogen :

L5178Y mouse T lymphoma cells expressing recombinant human FasL

Application Notes :

Additional reported applications (for the relevant formats) include: immunoprecipitation^1,2, immunofluorescence microscopy^3, immunocytochemistry^2, blocking of Fas induced apoptosis^1, and Western blotting^11. Fas Ligand is expressed at low density on activated cells. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 306407) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 306404) or biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by SAv-PE (Cat. No. 405204)). In addition, applying matrix metalloproteinases (MMPS) inhibitor in the cell culture system will increase the FasL staining intensity. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 306415 and 306416).;

more info or order :

BioLegend product webpage