LEAF™ Purified anti-human CD178 (Fas-L) | BioLegend 306409 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

LEAF™ Purified anti-human CD178 (Fas-L)

catalog :

306409

quantity :

500 μg

price :

290 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

NOK-1

reactivity :

human

application :

western blot, immunocytochemistry, immunoprecipitation, flow cytometry, blocking or activating experiments

citations: 4

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; loading ...; fig 3d	Tong A, Hashem H, Eid S, Allen F, Kingsley D, Huang A. Adoptive natural killer cell therapy is effective in reducing pulmonary metastasis of Ewing sarcoma. Oncoimmunology. 2017;6:e1303586 pubmed publisher
blocking or activating experiments; human; fig 4	Difranco K, Johnson Farley N, Bertino J, Elson D, Vega B, Belinka B, et al. LFA-1-targeting Leukotoxin (LtxA; LeukotheraÂ®) causes lymphoma tumor regression in a humanized mouse model and requires caspase-8 and Fas to kill malignant lymphocytes. Leuk Res. 2015;39:649-56 pubmed publisher
	Kabanova A, Sanseviero F, Candi V, Gamberucci A, Gozzetti A, Campoccia G, et al. Human Cytotoxic T Lymphocytes Form Dysfunctional Immune Synapses with B Cells Characterized by Non-Polarized Lytic Granule Release. Cell Rep. 2016;15:9-18 pubmed publisher
	Yin S, Zhang J, Mao Y, Hu Y, Cui L, Kang N, et al. Vav1-phospholipase C-?1 (Vav1-PLC-?1) pathway initiated by T cell antigen receptor (TCR??) activation is required to overcome inhibition by ubiquitin ligase Cbl-b during ??T cell cytotoxicity. J Biol Chem. 2013;288:26448-62 pubmed publisher

product information

Antigen :

CD178

Apps. Abbrev. :

Flow Cytometry, ICC, IP, Western Blotting

Cat # :

306409

Clone :

NOK-1

Item :

LEAF™ Purified anti-human CD178 (Fas-L)

Isotype :

Mouse IgG1, κ

Other Names :

Fas Ligand (FasL), CD95L, TNFSF6

Size :

500 μg

Price (USD) :

290 USD

Reactivity :

Human, Baboon

Clonality :

Monoclonal

Host :

Mouse

Conjugate/Tag/Label :

LEAF

Immunogen :

L5178Y mouse T lymphoma cells expressing recombinant human FasL

Application Notes :

Additional reported applications (for the relevant formats) include: immunoprecipitation 1,2 , immunofluorescence microscopy 3 , immunocytochemistry 2 , blocking of Fas induced apoptosis 1 , and Western blotting 11 . Fas Ligand is expressed at low density on activated cells. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 306407) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 306404) or biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by SAv-PE (Cat. No. 405204)). In addition, applying matrix metalloproteinases (MMPS) inhibitor in the cell culture system will increase the FasL staining intensity. The LEAF™ purified antibody (Endotoxin 0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 306409).