Purified anti-human CD178 (FasL) | BioLegend 306402 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

Purified anti-human CD178 (FasL)

catalog :

306402

quantity :

100 μg

price :

176 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

NOK-1

reactivity :

human

application :

western blot, immunocytochemistry, immunoprecipitation, flow cytometry, blocking or activating experiments

more info or order :

BioLegend product webpage

citations: 15

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; loading ...; fig 3d	Tong A, Hashem H, Eid S, Allen F, Kingsley D, Huang A. Adoptive natural killer cell therapy is effective in reducing pulmonary metastasis of Ewing sarcoma. Oncoimmunology. 2017;6:e1303586 pubmed publisher
blocking or activating experiments; human; fig 4	Difranco K, Johnson Farley N, Bertino J, Elson D, Vega B, Belinka B, et al. LFA-1-targeting Leukotoxin (LtxA; LeukotheraÂ®) causes lymphoma tumor regression in a humanized mouse model and requires caspase-8 and Fas to kill malignant lymphocytes. Leuk Res. 2015;39:649-56 pubmed publisher
	Lakshmikanth T, Muhammad S, Olin A, Chen Y, Mikes J, Fagerberg L, et al. Human Immune System Variation during 1 Year. Cell Rep. 2020;32:107923 pubmed publisher
	Svajger U, Rožman P. Synergistic Effects of Interferon-γ and Vitamin D3 Signaling in Induction of ILT-3highPDL-1high Tolerogenic Dendritic Cells. Front Immunol. 2019;10:2627 pubmed publisher
	Kong R, Duan H, Sheng Z, Xu K, Acharya P, Chen X, et al. Antibody Lineages with Vaccine-Induced Antigen-Binding Hotspots Develop Broad HIV Neutralization. Cell. 2019;178:567-584.e19 pubmed publisher
	Zimmerman M, Quicke K, O Neal J, Arora N, Machiah D, Priyamvada L, et al. Cross-Reactive Dengue Virus Antibodies Augment Zika Virus Infection of Human Placental Macrophages. Cell Host Microbe. 2018;24:731-742.e6 pubmed publisher
	Sung J, Patel S, Clohosey M, Roesch L, Tripic T, Kuruc J, et al. HIV-Specific, Ex Vivo Expanded T Cell Therapy: Feasibility, Safety, and Efficacy in ART-Suppressed HIV-Infected Individuals. Mol Ther. 2018;26:2496-2506 pubmed publisher
	Ma Y, Walsh M, Bernhardt K, Ashbaugh C, Trudeau S, Ashbaugh I, et al. CRISPR/Cas9 Screens Reveal Epstein-Barr Virus-Transformed B Cell Host Dependency Factors. Cell Host Microbe. 2017;21:580-591.e7 pubmed publisher
	Mooren F, Völker K, Klocke R, Nikol S, Waltenberger J, Krüger K. Exercise delays neutrophil apoptosis by a G-CSF-dependent mechanism. J Appl Physiol (1985). 2012;113:1082-90 pubmed publisher
	Shrestha B, Pinto A, Green S, Bosch I, Diamond M. CD8+ T cells use TRAIL to restrict West Nile virus pathogenesis by controlling infection in neurons. J Virol. 2012;86:8937-48 pubmed publisher
	Qin G, Liu Y, Zheng J, Xiang Z, Ng I, Malik Peiris J, et al. Phenotypic and functional characterization of human ?? T-cell subsets in response to influenza A viruses. J Infect Dis. 2012;205:1646-53 pubmed publisher
	Khalid M, Yu H, Sauter D, Usmani S, Schmökel J, Feldman J, et al. Efficient Nef-mediated downmodulation of TCR-CD3 and CD28 is associated with high CD4+ T cell counts in viremic HIV-2 infection. J Virol. 2012;86:4906-20 pubmed publisher
	Kruger K, Agnischock S, Lechtermann A, Tiwari S, Mishra M, Pilat C, et al. Intensive resistance exercise induces lymphocyte apoptosis via cortisol and glucocorticoid receptor-dependent pathways. J Appl Physiol (1985). 2011;110:1226-32 pubmed publisher
	Li J, D Alessio A, Pober J. Lipopolysaccharide can trigger a cathepsin B-dependent programmed death response in human endothelial cells. Am J Pathol. 2009;175:1124-35 pubmed publisher
	Strauss L, Bergmann C, Whiteside T. Human circulating CD4+CD25highFoxp3+ regulatory T cells kill autologous CD8+ but not CD4+ responder cells by Fas-mediated apoptosis. J Immunol. 2009;182:1469-80 pubmed

product information

Antigen :

CD178

Apps. Abbrev. :

FC, ICC, IP, WB

Cat # :

306402

Clone :

NOK-1

Item :

Purified anti-human CD178 (FasL)

Isotype :

Mouse IgG1, κ

Other Names :

Fas Ligand (FasL), CD95L, TNFSF6, Fas-L

Size :

100 μg

Price (USD) :

176 USD

Reactivity :

Human

Clonality :

Monoclonal

Host :

Mouse

Conjugate/Tag/Label :

PURE

Immunogen :

L5178Y mouse T lymphoma cells expressing recombinant human FasL

Application Notes :

Additional reported applications (for the relevant formats) include: immunoprecipitation^1,2, immunofluorescence microscopy^3, immunocytochemistry^2, blocking of Fas induced apoptosis^1, and Western blotting^11. Fas Ligand is expressed at low density on activated cells. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 306407) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 306404) or biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by SAv-PE (Cat. No. 405204)). In addition, applying matrix metalloproteinases (MMPS) inhibitor in the cell culture system will increase the FasL staining intensity. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 306415 and 306416).;

more info or order :

BioLegend product webpage