product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Purified anti-human CD123
catalog :
306002
quantity :
100 μg
price :
101 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
6H6
reactivity :
human, rhesus macaque
application :
western blot, immunoprecipitation, flow cytometry, immunohistochemistry - frozen section, mass cytometry
more info or order :
citations: 43
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - frozen section; human; 1:50; loading ...; fig 5d
Rosa T, Mendes M, Linhares N, Rodrigues T, Dias A, Leal Calvo T, et al. The Type I Interferon Pathway Is Upregulated in the Cutaneous Lesions and Blood of Multibacillary Leprosy Patients With Erythema Nodosum Leprosum. Front Med (Lausanne). 2022;9:899998 pubmed publisher
  • flow cytometry; human; loading ...; fig 7b
Beziat V, Tavernier S, Chen Y, Ma C, Materna M, Laurence A, et al. Dominant-negative mutations in human IL6ST underlie hyper-IgE syndrome. J Exp Med. 2020;217: pubmed publisher
  • flow cytometry; human; loading ...; fig 1b
Kim J, Jeong J, Jung J, Jeon H, Lee S, Lim J, et al. Immunological characteristics and possible pathogenic role of urinary CD11c+ macrophages in lupus nephritis. Rheumatology (Oxford). 2020;: pubmed publisher
  • flow cytometry; human; loading ...; fig 1f
Chen Y, Gomes T, Hardman C, Vieira Braga F, Gutowska Owsiak D, Salimi M, et al. Re-evaluation of human BDCA-2+ DC during acute sterile skin inflammation. J Exp Med. 2020;217: pubmed publisher
  • flow cytometry; human; loading ...; fig s2e
Martin J, Chang C, Boschetti G, Ungaro R, Giri M, Grout J, et al. Single-Cell Analysis of Crohn's Disease Lesions Identifies a Pathogenic Cellular Module Associated with Resistance to Anti-TNF Therapy. Cell. 2019;178:1493-1508.e20 pubmed publisher
  • mass cytometry; human; loading ...; fig 2j
Wagner J, Rapsomaniki M, Chevrier S, Anzeneder T, Langwieder C, Dykgers A, et al. A Single-Cell Atlas of the Tumor and Immune Ecosystem of Human Breast Cancer. Cell. 2019;177:1330-1345.e18 pubmed publisher
  • flow cytometry; rhesus macaque; loading ...; fig 2d
Chea L, Wyatt L, Gangadhara S, Moss B, Amara R. Novel Modified Vaccinia Virus Ankara Vector Expressing Anti-apoptotic Gene B13R Delays Apoptosis and Enhances Humoral Responses. J Virol. 2019;93: pubmed publisher
  • flow cytometry; human; loading ...; tbl s1
Dias J, Boulouis C, Gorin J, van den Biggelaar R, Lal K, Gibbs A, et al. The CD4-CD8- MAIT cell subpopulation is a functionally distinct subset developmentally related to the main CD8+ MAIT cell pool. Proc Natl Acad Sci U S A. 2018;115:E11513-E11522 pubmed publisher
  • flow cytometry; human; loading ...; fig 1a
Olin A, Henckel E, Chen Y, Lakshmikanth T, Pou C, Mikes J, et al. Stereotypic Immune System Development in Newborn Children. Cell. 2018;174:1277-1292.e14 pubmed publisher
  • flow cytometry; human; loading ...
Wang Y, Ma C, Ling Y, Bousfiha A, Camcioglu Y, Jacquot S, et al. Dual T cell- and B cell-intrinsic deficiency in humans with biallelic RLTPR mutations. J Exp Med. 2016;213:2413-2435 pubmed
  • flow cytometry; human; loading ...; fig 1a
Bal S, Bernink J, Nagasawa M, Groot J, Shikhagaie M, Golebski K, et al. IL-1?, IL-4 and IL-12 control the fate of group 2 innate lymphoid cells in human airway inflammation in the lungs. Nat Immunol. 2016;17:636-45 pubmed publisher
  • flow cytometry; human; 1:80
Lee J, Breton G, Aljoufi A, Zhou Y, PUHR S, Nussenzweig M, et al. Clonal analysis of human dendritic cell progenitor using a stromal cell culture. J Immunol Methods. 2015;425:21-6 pubmed publisher
Poon M, Caron D, Wang Z, Wells S, Chen D, Meng W, et al. Tissue adaptation and clonal segregation of human memory T cells in barrier sites. Nat Immunol. 2023;24:309-319 pubmed publisher
Laggner M, Acosta G, Kitzm xfc ller C, Copic D, Gruber F, Altenburger L, et al. The secretome of irradiated peripheral blood mononuclear cells attenuates activation of mast cells and basophils. EBioMedicine. 2022;81:104093 pubmed publisher
McCarthy E, Odorizzi P, Lutz E, Smullin C, Tenvooren I, Stone M, et al. A cytotoxic-skewed immune set point predicts low neutralizing antibody levels after Zika virus infection. Cell Rep. 2022;39:110815 pubmed publisher
Baskar R, Chen A, Favaro P, Reynolds W, Mueller F, Borges L, et al. Integrating transcription-factor abundance with chromatin accessibility in human erythroid lineage commitment. Cell Rep Methods. 2022;2: pubmed publisher
Vivanco Gonzalez N, Oliveria J, Tebaykin D, Ivison G, Mukai K, Tsai M, et al. An optimized protocol for phenotyping human granulocytes by mass cytometry. STAR Protoc. 2022;3:101280 pubmed publisher
. A blood atlas of COVID-19 defines hallmarks of disease severity and specificity. Cell. 2022;185:916-938.e58 pubmed publisher
Leader A, Grout J, Maier B, Nabet B, Park M, Tabachnikova A, et al. Single-cell analysis of human non-small cell lung cancer lesions refines tumor classification and patient stratification. Cancer Cell. 2021;39:1594-1609.e12 pubmed publisher
Syrimi E, Fennell E, Richter A, Vrljicak P, Stark R, Ott S, et al. The immune landscape of SARS-CoV-2-associated Multisystem Inflammatory Syndrome in Children (MIS-C) from acute disease to recovery. iScience. 2021;24:103215 pubmed publisher
Henrick B, Rodriguez L, Lakshmikanth T, Pou C, Henckel E, Arzoomand A, et al. Bifidobacteria-mediated immune system imprinting early in life. Cell. 2021;184:3884-3898.e11 pubmed publisher
Saygin C, Hu E, Zhang P, Sher S, Lozanski A, Doong T, et al. Genomic analysis of cellular hierarchy in acute myeloid leukemia using ultrasensitive LC-FACSeq. Leukemia. 2021;35:3406-3420 pubmed publisher
Kato M, Ikeda K, Sugiyama T, Tanaka S, Iida K, Suga K, et al. Associations of ultrasound-based inflammation patterns with peripheral innate lymphoid cell populations, serum cytokines/chemokines, and treatment response to methotrexate in rheumatoid arthritis and spondyloarthritis. PLoS ONE. 2021;16:e0252116 pubmed publisher
Roussel M, Ferrant J, Reizine F, Le Gallou S, Dulong J, Carl S, et al. Comparative immune profiling of acute respiratory distress syndrome patients with or without SARS-CoV-2 infection. Cell Rep Med. 2021;2:100291 pubmed publisher
Kaufmann M, Evans H, Schaupp A, Engler J, Kaur G, Willing A, et al. Identifying CNS-colonizing T cells as potential therapeutic targets to prevent progression of multiple sclerosis. Med (N Y). 2021;2:296-312.e8 pubmed publisher
Chevrier S, Zurbuchen Y, Cervia C, Adamo S, Raeber M, de Souza N, et al. A distinct innate immune signature marks progression from mild to severe COVID-19. Cell Rep Med. 2021;2:100166 pubmed publisher
Liu S, Grantham A, Landry C, Granda B, Chopra R, Chakravarthy S, et al. A CRISPR Screen Reveals Resistance Mechanisms to CD3-Bispecific Antibody Therapy. Cancer Immunol Res. 2021;9:34-49 pubmed publisher
Thrash E, Kleinsteuber K, Hathaway E, Nazzaro M, Haas E, Hodi F, et al. High-Throughput Mass Cytometry Staining for Immunophenotyping Clinical Samples. STAR Protoc. 2020;1:100055 pubmed publisher
Mann E, Menon M, Knight S, Konkel J, Jagger C, Shaw T, et al. Longitudinal immune profiling reveals key myeloid signatures associated with COVID-19. Sci Immunol. 2020;5: pubmed publisher
Kremenovic M, Rombini N, Chan A, Gruber T, Bäriswyl L, Lee D, et al. Characterization of a Myeloid Activation Signature that Correlates with Survival in Melanoma Patients. Cancers (Basel). 2020;12: pubmed publisher
Friebel E, Kapolou K, Unger S, Núñez N, Utz S, Rushing E, et al. Single-Cell Mapping of Human Brain Cancer Reveals Tumor-Specific Instruction of Tissue-Invading Leukocytes. Cell. 2020;181:1626-1642.e20 pubmed publisher
Parackova Z, Vrabcová P, Zentsova I, Kayserova J, Richtrova I, Sojka L, et al. Enhanced STAT3 phosphorylation and PD-L1 expression in myeloid dendritic cells indicate impaired IL-27Ralpha signaling in type 1 diabetes. Sci Rep. 2020;10:493 pubmed publisher
Eccles J, Turner R, Kirk N, Muehling L, Borish L, Steinke J, et al. T-bet+ Memory B Cells Link to Local Cross-Reactive IgG upon Human Rhinovirus Infection. Cell Rep. 2020;30:351-366.e7 pubmed publisher
O Boyle K, Ohtani T, Manne S, Bengsch B, Henrickson S, Wherry E, et al. Exploration of T-Cell Diversity Using Mass Cytometry. Methods Mol Biol. 2020;2111:1-20 pubmed publisher
Tripathi T, Yin W, Xue Y, Zurawski S, Fujita H, Hanabuchi S, et al. Central Roles of OX40L-OX40 Interaction in the Induction and Progression of Human T Cell-Driven Acute Graft-versus-Host Disease. Immunohorizons. 2019;3:110-120 pubmed publisher
Li Y, Teteloshvili N, Tan S, Rao S, Han A, Yang Y, et al. Humanized Mice Reveal New Insights Into the Thymic Selection of Human Autoreactive CD8+ T Cells. Front Immunol. 2019;10:63 pubmed publisher
Chevrier S, Crowell H, Zanotelli V, Engler S, Robinson M, Bodenmiller B. Compensation of Signal Spillover in Suspension and Imaging Mass Cytometry. Cell Syst. 2018;6:612-620.e5 pubmed publisher
Evrard M, Kwok I, Chong S, Teng K, Becht E, Chen J, et al. Developmental Analysis of Bone Marrow Neutrophils Reveals Populations Specialized in Expansion, Trafficking, and Effector Functions. Immunity. 2018;48:364-379.e8 pubmed publisher
Lavin Y, Kobayashi S, Leader A, Amir E, Elefant N, Bigenwald C, et al. Innate Immune Landscape in Early Lung Adenocarcinoma by Paired Single-Cell Analyses. Cell. 2017;169:750-765.e17 pubmed publisher
Dallari S, Macal M, Loureiro M, Jo Y, Swanson L, Hesser C, et al. Src family kinases Fyn and Lyn are constitutively activated and mediate plasmacytoid dendritic cell responses. Nat Commun. 2017;8:14830 pubmed publisher
Liu Y, Xing X, Wang J, Xing L, Su Y, Yao Z, et al. Sterigmatocystin alters the number of FoxP3+ regulatory T cells and plasmacytoid dendritic cells in BALB/c mice. Food Chem Toxicol. 2012;50:1920-6 pubmed publisher
Martin Gayo E, Sierra Filardi E, Corbi A, Toribio M. Plasmacytoid dendritic cells resident in human thymus drive natural Treg cell development. Blood. 2010;115:5366-75 pubmed publisher
Chen S, de Groot M, Kinsley D, Laverty M, McClanahan T, Arreaza M, et al. Expression of chemokine receptor CXCR3 by lymphocytes and plasmacytoid dendritic cells in human psoriatic lesions. Arch Dermatol Res. 2010;302:113-23 pubmed publisher
product information
Antigen :
CD123
Apps. Abbrev. :
FC, CyTOF®, IHC-F, IP, WB
Cat # :
306002
Clone :
6H6
Item :
Purified anti-human CD123
Isotype :
Mouse IgG1, κ
Other Names :
IL-3Rα, IL-3 Receptor alpha
Size :
100 μg
Price (USD) :
101 USD
Reactivity :
Human
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
PURE
Immunogen :
Human IL-3Rα transfected COS cells.
Application Notes :
Clone 6H6 does not inhibit IL-3 binding to low- or high-affinity IL-3Rs. Additional reported applications (for the relevant formats) include: Western blotting^1, immunoprecipitation^1, and immunohistochemical staining of acetone-fixed frozen sections^2 and also paraformaldehyde fixed paraffin embedded tissue^7.;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA