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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
PE anti-human CD86
catalog :
305438
quantity :
100 μg
price :
292 USD
clonality :
monoclonal
host :
mouse
conjugate :
PE
clone name :
IT2.2
reactivity :
human, rhesus macaque
application :
flow cytometry
more info or order :
citations: 54
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; loading ...; fig 4d
Chen Y, Gomes T, Hardman C, Vieira Braga F, Gutowska Owsiak D, Salimi M, et al. Re-evaluation of human BDCA-2+ DC during acute sterile skin inflammation. J Exp Med. 2020;217: pubmed publisher
  • flow cytometry; human; loading ...; fig 2a
Gu C, Wang L, Zurawski S, Oh S. Signaling Cascade through DC-ASGPR Induces Transcriptionally Active CREB for IL-10 Induction and Immune Regulation. J Immunol. 2019;: pubmed publisher
  • flow cytometry; human; loading ...; fig 3a
Allden S, Ogger P, Ghai P, McErlean P, Hewitt R, Toshner R, et al. The Transferrin Receptor CD71 Delineates Functionally Distinct Airway Macrophage Subsets during Idiopathic Pulmonary Fibrosis. Am J Respir Crit Care Med. 2019;: pubmed publisher
  • flow cytometry; human; loading ...; fig 1b
Tremblay McLean A, Coenraads S, Kiani Z, Dupuy F, Bernard N. Expression of ligands for activating natural killer cell receptors on cell lines commonly used to assess natural killer cell function. BMC Immunol. 2019;20:8 pubmed publisher
  • flow cytometry; human; loading ...; fig 7c
Zanetti S, Ziblat A, Torres N, Zwirner N, Bouzat C. Expression and Functional Role of ?7 Nicotinic Receptor in Human Cytokine-stimulated Natural Killer (NK) Cells. J Biol Chem. 2016;291:16541-52 pubmed publisher
  • flow cytometry; human; fig 7
Phadnis Moghe A, Crawford R, Kaminski N. Suppression of human B cell activation by 2,3,7,8-tetrachlorodibenzo-p-dioxin involves altered regulation of B cell lymphoma-6. Toxicol Sci. 2015;144:39-50 pubmed publisher
  • flow cytometry; human
Ziblat A, Domaica C, Spallanzani R, Iraolagoitia X, Rossi L, Avila D, et al. IL-27 stimulates human NK-cell effector functions and primes NK cells for IL-18 responsiveness. Eur J Immunol. 2015;45:192-202 pubmed publisher
  • flow cytometry; human
Koido S, Homma S, Okamoto M, Takakura K, Mori M, Yoshizaki S, et al. Treatment with chemotherapy and dendritic cells pulsed with multiple Wilms' tumor 1 (WT1)-specific MHC class I/II-restricted epitopes for pancreatic cancer. Clin Cancer Res. 2014;20:4228-39 pubmed publisher
Guo Q, Chen W, Sun J, Zhao C, Bai X, Zhang Y, et al. Nocardia rubra cell-wall skeleton activates an immune response in cervical tissue via stimulating FPR3 to enhance dendritic cell-mediated Th1 differentiation. Front Immunol. 2023;14:1117545 pubmed publisher
Miller R, Luke J, Hu S, Mahabhashyam S, Jones W, Marron T, et al. Anti-CD73 antibody activates human B cells, enhances humoral responses and induces redistribution of B cells in patients with cancer. J Immunother Cancer. 2022;10: pubmed publisher
Sugrue J, Posseme C, Tan Z, Pou C, Charbit B, Bondet V, et al. Enhanced TLR3 responsiveness in hepatitis C virus resistant women from the Irish anti-D cohort. Cell Rep Med. 2022;3:100804 pubmed publisher
De Le xf3 n Rodr xed guez S, Hern xe1 ndez Herrera P, Aguilar Flores C, P xe9 rez Koldenkova V, Guerrero A, Mantilla A, et al. A Machine Learning Workflow of Multiplexed Immunofluorescence Images to Interrogate Activator and Tolerogenic Profiles of Conventional Type 1 Dendritic Cells Infiltrating Melanomas of Disease-Free and Metastatic Patients. J Oncol. 2022;2022:9775736 pubmed publisher
de Groot A, Myers K, Krueger T, Brennen W, Amend S, Pienta K. Targeting interleukin 4 receptor alpha on tumor-associated macrophages reduces the pro-tumor macrophage phenotype. Neoplasia. 2022;32:100830 pubmed publisher
Herati R, Knorr D, Vella L, Silva L, Chilukuri L, Apostolidis S, et al. PD-1 directed immunotherapy alters Tfh and humoral immune responses to seasonal influenza vaccine. Nat Immunol. 2022;23:1183-1192 pubmed publisher
Schimek V, Strasser K, Beer A, Göber S, Walterskirchen N, Brostjan C, et al. Tumour cell apoptosis modulates the colorectal cancer immune microenvironment via interleukin-8-dependent neutrophil recruitment. Cell Death Dis. 2022;13:113 pubmed publisher
Hu X, Zhang A, Pan W, Xin Q, Chen J, Zhang L, et al. An IgD-Fc-Ig fusion protein restrains the activation of T and B cells by inhibiting IgD-IgDR-Lck signaling in rheumatoid arthritis. Acta Pharmacol Sin. 2022;43:387-400 pubmed publisher
Chen R, Huang M, Yang X, Chen X, Shi M, Li Z, et al. CALR-TLR4 Complex Inhibits Non-Small Cell Lung Cancer Progression by Regulating the Migration and Maturation of Dendritic Cells. Front Oncol. 2021;11:743050 pubmed publisher
Wang X, Zhang H, Ge Y, Cao L, He Y, Sun G, et al. AT1R Regulates Macrophage Polarization Through YAP and Regulates Aortic Dissection Incidence. Front Physiol. 2021;12:644903 pubmed publisher
Hofbauer D, Mougiakakos D, Broggini L, Zaiss M, B xfc ttner Herold M, Bach C, et al. β2-microglobulin triggers NLRP3 inflammasome activation in tumor-associated macrophages to promote multiple myeloma progression. Immunity. 2021;54:1772-1787.e9 pubmed publisher
Que Y, Li H, Lin L, Zhu X, Xiao M, Wang Y, et al. Study on the Immune Escape Mechanism of Acute Myeloid Leukemia With DNMT3A Mutation. Front Immunol. 2021;12:653030 pubmed publisher
Li X, Zheng J, Chen S, Meng F, Ning J, Sun S. Oleandrin, a cardiac glycoside, induces immunogenic cell death via the PERK/elF2α/ATF4/CHOP pathway in breast cancer. Cell Death Dis. 2021;12:314 pubmed publisher
Wildner N, Ahmadi P, Schulte S, Brauneck F, Kohsar M, L xfc tgehetmann M, et al. B cell analysis in SARS-CoV-2 versus malaria: Increased frequencies of plasmablasts and atypical memory B cells in COVID-19. J Leukoc Biol. 2021;109:77-90 pubmed publisher
Luvanda M, Posch W, Vosper J, Zaderer V, Noureen A, Lass Fl xf6 rl C, et al. Dexamethasone Promotes Aspergillus fumigatus Growth in Macrophages by Triggering M2 Repolarization via Targeting PKM2. J Fungi (Basel). 2021;7: pubmed publisher
Casasola LaMacchia A, Ritorto M, Seward R, Ahyi Amendah N, Ciarla A, Hickling T, et al. Human leukocyte antigen class II quantification by targeted mass spectrometry in dendritic-like cell lines and monocyte-derived dendritic cells. Sci Rep. 2021;11:1028 pubmed publisher
Koh W, Zayats R, López P, Murooka T. Visualizing Cellular Dynamics and Protein Localization in 3D Collagen. STAR Protoc. 2020;1:100203 pubmed publisher
Oulès B, Philippeos C, Segal J, Tihy M, Vietri Rudan M, Cujba A, et al. Contribution of GATA6 to homeostasis of the human upper pilosebaceous unit and acne pathogenesis. Nat Commun. 2020;11:5067 pubmed publisher
Kan S, Bito T, Shimabuku M, Taguchi J, Ohkusa T, Shimodaira S, et al. Impact of mature dendritic cells pulsed with a novel WT1 helper peptide on the induction of HLA‑A2‑restricted WT1‑reactive CD8+ T cells. Int J Oncol. 2020;57:1047-1056 pubmed publisher
Laing A, Lorenc A, Del Molino Del Barrio I, Das A, Fish M, Monin L, et al. A dynamic COVID-19 immune signature includes associations with poor prognosis. Nat Med. 2020;26:1623-1635 pubmed publisher
Jankeel A, Menicucci A, Woolsey C, Fenton K, Mendoza N, Versteeg K, et al. Early Transcriptional Changes within Liver, Adrenal Gland, and Lymphoid Tissues Significantly Contribute to Ebola Virus Pathogenesis in Cynomolgus Macaques. J Virol. 2020;94: pubmed publisher
Yamada K, Heim C, Xi X, Attri K, Wang D, Zhang W, et al. Monocyte metabolic reprogramming promotes pro-inflammatory activity and Staphylococcus aureus biofilm clearance. PLoS Pathog. 2020;16:e1008354 pubmed publisher
Haruna M, Hirata M, Iwahori K, Kanazawa T, Yamamoto Y, Goto K, et al. Docetaxel Upregulates HMGB1 Levels in Non-small Cell Lung Cancer. Biol Pharm Bull. 2020;43:399-403 pubmed publisher
Welch J, Kaufman T, Stapleton J, Okeoma C. Semen exosomes inhibit HIV infection and HIV-induced proinflammatory cytokine production independent of the activation state of primary lymphocytes. FEBS Lett. 2020;594:695-709 pubmed publisher
Wang X, Sun G, Feng T, Zhang J, Huang X, Wang T, et al. Sodium oligomannate therapeutically remodels gut microbiota and suppresses gut bacterial amino acids-shaped neuroinflammation to inhibit Alzheimer's disease progression. Cell Res. 2019;29:787-803 pubmed publisher
Sugawara E, Kato M, Kudo Y, Lee W, Hisada R, Fujieda Y, et al. Autophagy promotes citrullination of VIM (vimentin) and its interaction with major histocompatibility complex class II in synovial fibroblasts. Autophagy. 2020;16:946-955 pubmed publisher
Mendoza Cabrera M, Navarro Hernández R, Santerre A, Ortiz Lazareno P, Pereira Suárez A, Estrada Chávez C. Effect of pregnancy hormone mixtures on cytokine production and surface marker expression in naïve and LPS-activated THP-1 differentiated monocytes/macrophages. Innate Immun. 2020;26:84-96 pubmed publisher
Bryson B, Rosebrock T, Tafesse F, Itoh C, Nibasumba A, Babunovic G, et al. Heterogeneous GM-CSF signaling in macrophages is associated with control of Mycobacterium tuberculosis. Nat Commun. 2019;10:2329 pubmed publisher
Blaszczak A, Jalilvand A, Liu J, Wright V, Suzo A, Needleman B, et al. Human Visceral Adipose Tissue Macrophages Are Not Adequately Defined by Standard Methods of Characterization. J Diabetes Res. 2019;2019:8124563 pubmed publisher
Su S, Zhao J, Xing Y, Zhang X, Liu J, Ouyang Q, et al. Immune Checkpoint Inhibition Overcomes ADCP-Induced Immunosuppression by Macrophages. Cell. 2018;175:442-457.e23 pubmed publisher
Lin J, Izar B, Wang S, Yapp C, Mei S, Shah P, et al. Highly multiplexed immunofluorescence imaging of human tissues and tumors using t-CyCIF and conventional optical microscopes. elife. 2018;7: pubmed publisher
Du X, Tang F, Liu M, Su J, Zhang Y, Wu W, et al. A reappraisal of CTLA-4 checkpoint blockade in cancer immunotherapy. Cell Res. 2018;28:416-432 pubmed publisher
Philippeos C, Telerman S, Oulès B, Pisco A, Shaw T, Elgueta R, et al. Spatial and Single-Cell Transcriptional Profiling Identifies Functionally Distinct Human Dermal Fibroblast Subpopulations. J Invest Dermatol. 2018;138:811-825 pubmed publisher
Bermúdez Brito M, Faas M, De Vos P. Modulation of Dendritic-Epithelial Cell Responses against Sphingomonas Paucimobilis by Dietary Fibers. Sci Rep. 2016;6:30277 pubmed publisher
Barman S, Kayama H, Okuzaki D, Ogino T, Osawa H, Matsuno H, et al. Identification of a human intestinal myeloid cell subset that regulates gut homeostasis. Int Immunol. 2016;28:533-545 pubmed
Li H, Borrego F, Nagata S, Tolnay M. Fc Receptor-like 5 Expression Distinguishes Two Distinct Subsets of Human Circulating Tissue-like Memory B Cells. J Immunol. 2016;196:4064-74 pubmed publisher
Macal M, Tam M, Hesser C, Di Domizio J, Léger P, Gilliet M, et al. CD28 Deficiency Enhances Type I IFN Production by Murine Plasmacytoid Dendritic Cells. J Immunol. 2016;196:1900-9 pubmed publisher
Finotti G, Tamassia N, Calzetti F, Fattovich G, Cassatella M. Endogenously produced TNF-α contributes to the expression of CXCL10/IP-10 in IFN-λ3-activated plasmacytoid dendritic cells. J Leukoc Biol. 2016;99:107-19 pubmed publisher
Wee Y, Jung J, Kim Y, Choi M, Kim Y, Choi D, et al. Involvement of indirectly allostimulated CD4+CD43highCD45RO+ T cell proliferation in the development of chronic allograft nephropathy. Exp Biol Med (Maywood). 2016;241:1217-28 pubmed publisher
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Seeger P, Bosisio D, Parolini S, Badolato R, Gismondi A, Santoni A, et al. Activin A as a mediator of NK-dendritic cell functional interactions. J Immunol. 2014;192:1241-8 pubmed publisher
Wang X, Barnes P, Huang F, Alvarez I, Neuenschwander P, Sherman D, et al. Early secreted antigenic target of 6-kDa protein of Mycobacterium tuberculosis primes dendritic cells to stimulate Th17 and inhibit Th1 immune responses. J Immunol. 2012;189:3092-103 pubmed publisher
Stoeker L, Nordone S, Gunderson S, Zhang L, Kajikawa A, Lavoy A, et al. Assessment of Lactobacillus gasseri as a candidate oral vaccine vector. Clin Vaccine Immunol. 2011;18:1834-44 pubmed publisher
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Bosco M, Pierobon D, Blengio F, Raggi F, Vanni C, Gattorno M, et al. Hypoxia modulates the gene expression profile of immunoregulatory receptors in human mature dendritic cells: identification of TREM-1 as a novel hypoxic marker in vitro and in vivo. Blood. 2011;117:2625-39 pubmed publisher
Fiola S, Gosselin D, Takada K, Gosselin J. TLR9 contributes to the recognition of EBV by primary monocytes and plasmacytoid dendritic cells. J Immunol. 2010;185:3620-31 pubmed publisher
product information
Antigen :
CD86
Apps. Abbrev. :
FC
Cat # :
305438
Clone :
IT2.2
Item :
PE anti-human CD86
Isotype :
Mouse IgG2b, κ
Other Names :
B7-2, B70
Size :
100 μg
Price (USD) :
292 USD
Reactivity :
Human, Cynomolgus, Rhesus
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
PE
Application Notes :
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections^6, Western blotting^3, and blocking of T cell activation^2,4,5. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 305449 & 305450).;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA