product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Alexa Fluor® 488 anti-human CD86
catalog :
305414
quantity :
100 tests
price :
265 USD
clonality :
monoclonal
host :
mouse
conjugate :
AF488
clone name :
IT2.2
reactivity :
human, rhesus macaque
application :
flow cytometry
more info or order :
citations: 20
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; loading ...; fig s1c
Morrissey M, Byrne R, Nulty C, McCabe N, Lynam Lennon N, Butler C, et al. The tumour microenvironment of the upper and lower gastrointestinal tract differentially influences dendritic cell maturation. BMC Cancer. 2020;20:566 pubmed publisher
  • flow cytometry; human; loading ...; fig 2c, 4b
Kim J, Jeong J, Jung J, Jeon H, Lee S, Lim J, et al. Immunological characteristics and possible pathogenic role of urinary CD11c+ macrophages in lupus nephritis. Rheumatology (Oxford). 2020;: pubmed publisher
  • flow cytometry; human; loading ...; fig 4d
Chen Y, Gomes T, Hardman C, Vieira Braga F, Gutowska Owsiak D, Salimi M, et al. Re-evaluation of human BDCA-2+ DC during acute sterile skin inflammation. J Exp Med. 2020;217: pubmed publisher
  • flow cytometry; human; loading ...; fig 3a
Allden S, Ogger P, Ghai P, McErlean P, Hewitt R, Toshner R, et al. The Transferrin Receptor CD71 Delineates Functionally Distinct Airway Macrophage Subsets during Idiopathic Pulmonary Fibrosis. Am J Respir Crit Care Med. 2019;: pubmed publisher
  • flow cytometry; human; loading ...; fig 1b
Tremblay McLean A, Coenraads S, Kiani Z, Dupuy F, Bernard N. Expression of ligands for activating natural killer cell receptors on cell lines commonly used to assess natural killer cell function. BMC Immunol. 2019;20:8 pubmed publisher
  • flow cytometry; human; loading ...; fig 1b
Richardson J, Armbruster N, Günter M, Henes J, Autenrieth S. Staphylococcus aureus PSM Peptides Modulate Human Monocyte-Derived Dendritic Cells to Prime Regulatory T Cells. Front Immunol. 2018;9:2603 pubmed publisher
  • flow cytometry; human; loading ...; fig 5b
Otsuka Y, Watanabe E, Shinya E, Okura S, Saeki H, Geijtenbeek T, et al. Differentiation of Langerhans Cells from Monocytes and Their Specific Function in Inducing IL-22-Specific Th Cells. J Immunol. 2018;201:3006-3016 pubmed publisher
  • flow cytometry; human; loading ...; fig 2c
Melo Gonzalez F, Fenton T, Forss C, Smedley C, Goenka A, MacDonald A, et al. Intestinal mucin activates human dendritic cells and IL-8 production in a glycan-specific manner. J Biol Chem. 2018;293:8543-8553 pubmed publisher
  • flow cytometry; human; 1:50; loading ...; fig s1d
Smith N, Pietrancosta N, Davidson S, Dutrieux J, Chauveau L, Cutolo P, et al. Natural amines inhibit activation of human plasmacytoid dendritic cells through CXCR4 engagement. Nat Commun. 2017;8:14253 pubmed publisher
  • flow cytometry; human; fig s4b
Martin Gayo E, Cronin J, Hickman T, Ouyang Z, Lindqvist M, Kolb K, et al. Circulating CXCR5+CXCR3+PD-1lo Tfh-like cells in HIV-1 controllers with neutralizing antibody breadth. JCI Insight. 2017;2:e89574 pubmed publisher
  • flow cytometry; human; loading ...; fig s9a
Tomic A, Varanasi P, Golemac M, Malic S, Riese P, Borst E, et al. Activation of Innate and Adaptive Immunity by a Recombinant Human Cytomegalovirus Strain Expressing an NKG2D Ligand. PLoS Pathog. 2016;12:e1006015 pubmed publisher
  • flow cytometry; human; loading ...; fig 2a
Cheng W, van Asten S, Burns L, Evans H, Walter G, Hashim A, et al. Periodontitis-associated pathogens P. gingivalis and A. actinomycetemcomitans activate human CD14(+) monocytes leading to enhanced Th17/IL-17 responses. Eur J Immunol. 2016;46:2211-21 pubmed publisher
  • flow cytometry; human; loading ...; fig 7c
Zanetti S, Ziblat A, Torres N, Zwirner N, Bouzat C. Expression and Functional Role of ?7 Nicotinic Receptor in Human Cytokine-stimulated Natural Killer (NK) Cells. J Biol Chem. 2016;291:16541-52 pubmed publisher
  • flow cytometry; human; loading ...; fig 2a
Svajger U, Obermajer N, Jeras M. IFN-?-rich environment programs dendritic cells toward silencing of cytotoxic immune responses. J Leukoc Biol. 2014;95:33-46 pubmed publisher
Pedicona F, Casado P, Hijazi M, Gribben J, Rouault Pierre K, Cutillas P. Targeting the lysine-specific demethylase 1 rewires kinase networks and primes leukemia cells for kinase inhibitor treatment. Sci Signal. 2022;15:eabl7989 pubmed publisher
Qian S, Han X, Sha X, Tian F, Huang H, Jiang P, et al. Aqueous Extract of Cimicifuga dahurica Reprogramming Macrophage Polarization by Activating TLR4-NF-κB Signaling Pathway. J Inflamm Res. 2022;15:1027-1046 pubmed publisher
Walter F, Winter E, Rahn S, Heidland J, Meier S, Struzek A, et al. Chitosan nanoparticles as antigen vehicles to induce effective tumor specific T cell responses. PLoS ONE. 2020;15:e0239369 pubmed publisher
Yu X, Chan H, Fisher H, Penfold C, Kim J, Inzhelevskaya T, et al. Isotype Switching Converts Anti-CD40 Antagonism to Agonism to Elicit Potent Antitumor Activity. Cancer Cell. 2020;37:850-866.e7 pubmed publisher
Findlay E, Currie A, Zhang A, Ovciarikova J, Young L, Stevens H, et al. Exposure to the antimicrobial peptide LL-37 produces dendritic cells optimized for immunotherapy. Oncoimmunology. 2019;8:1608106 pubmed publisher
Mackroth M, Abel A, Steeg C, Schulze zur Wiesch J, Jacobs T. Acute Malaria Induces PD1+CTLA4+ Effector T Cells with Cell-Extrinsic Suppressor Function. PLoS Pathog. 2016;12:e1005909 pubmed publisher
product information
Antigen :
CD86
Apps. Abbrev. :
FC
Cat # :
305414
Clone :
IT2.2
Item :
Alexa Fluor® 488 anti-human CD86
Isotype :
Mouse IgG2b, κ
Other Names :
B7-2, B70
Size :
100 tests
Price (USD) :
265 USD
Reactivity :
Human, Cynomolgus, Rhesus
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
A488
Application Notes :
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections^6, Western blotting^3, and blocking of T cell activation^2,4,5. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 305449 & 305450).;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA