This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
LEAF™ Purified anti-human CD86
catalog :
305410
quantity :
500 μg
price :
320 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
IT2.2
reactivity :
human, mouse, chimpanzee, equine, Rhesus monkey
application :
western blot, flow cytometry, immunohistochemistry - frozen section, blocking or activating experiments
citations: 30
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; loading ...; fig 1b
Richardson J, Armbruster N, Günter M, Henes J, Autenrieth S. Staphylococcus aureus PSM Peptides Modulate Human Monocyte-Derived Dendritic Cells to Prime Regulatory T Cells. Front Immunol. 2018;9:2603 pubmed publisher
  • flow cytometry; human; loading ...; fig 5b
Otsuka Y, Watanabe E, Shinya E, Okura S, Saeki H, Geijtenbeek T, et al. Differentiation of Langerhans Cells from Monocytes and Their Specific Function in Inducing IL-22-Specific Th Cells. J Immunol. 2018;201:3006-3016 pubmed publisher
  • flow cytometry; human; loading ...; fig 2c
Melo Gonzalez F, Fenton T, Forss C, Smedley C, Goenka A, MacDonald A, et al. Intestinal mucin activates human dendritic cells and IL-8 production in a glycan-specific manner. J Biol Chem. 2018;293:8543-8553 pubmed publisher
  • flow cytometry; human; fig 1b
Tyler C, McCarthy N, Lindsay J, Stagg A, Moser B, Eberl M. Antigen-Presenting Human γδ T Cells Promote Intestinal CD4+ T Cell Expression of IL-22 and Mucosal Release of Calprotectin. J Immunol. 2017;198:3417-3425 pubmed publisher
  • flow cytometry; human; 1:50; loading ...; fig s1d
Smith N, Pietrancosta N, Davidson S, Dutrieux J, Chauveau L, Cutolo P, et al. Natural amines inhibit activation of human plasmacytoid dendritic cells through CXCR4 engagement. Nat Commun. 2017;8:14253 pubmed publisher
  • flow cytometry; human; fig s4b
Martin Gayo E, Cronin J, Hickman T, Ouyang Z, Lindqvist M, Kolb K, et al. Circulating CXCR5+CXCR3+PD-1lo Tfh-like cells in HIV-1 controllers with neutralizing antibody breadth. JCI Insight. 2017;2:e89574 pubmed publisher
  • flow cytometry; Rhesus monkey; loading ...
Kasturi S, Kozlowski P, Nakaya H, Burger M, Russo P, Pham M, et al. Adjuvanting a Simian Immunodeficiency Virus Vaccine with Toll-Like Receptor Ligands Encapsulated in Nanoparticles Induces Persistent Antibody Responses and Enhanced Protection in TRIM5α Restrictive Macaques. J Virol. 2017;91: pubmed publisher
  • flow cytometry; human; loading ...; fig s9a
Tomic A, Varanasi P, Golemac M, Malic S, Riese P, Borst E, et al. Activation of Innate and Adaptive Immunity by a Recombinant Human Cytomegalovirus Strain Expressing an NKG2D Ligand. PLoS Pathog. 2016;12:e1006015 pubmed publisher
  • flow cytometry; human; loading ...
Ju X, Silveira P, Hsu W, Elgundi Z, Alingcastre R, Verma N, et al. The Analysis of CD83 Expression on Human Immune Cells Identifies a Unique CD83+-Activated T Cell Population. J Immunol. 2016;197:4613-4625 pubmed
  • flow cytometry; human; loading ...; fig 4a
Carroll V, Lafferty M, Marchionni L, Bryant J, Gallo R, Garzino Demo A. Expression of HIV-1 matrix protein p17 and association with B-cell lymphoma in HIV-1 transgenic mice. Proc Natl Acad Sci U S A. 2016;113:13168-13173 pubmed
  • flow cytometry; human; loading ...; fig 2a
Cheng W, van Asten S, Burns L, Evans H, Walter G, Hashim A, et al. Periodontitis-associated pathogens P. gingivalis and A. actinomycetemcomitans activate human CD14(+) monocytes leading to enhanced Th17/IL-17 responses. Eur J Immunol. 2016;46:2211-21 pubmed publisher
  • flow cytometry; human; loading ...; fig 7c
Zanetti S, Ziblat A, Torres N, Zwirner N, Bouzat C. Expression and Functional Role of ?7 Nicotinic Receptor in Human Cytokine-stimulated Natural Killer (NK) Cells. J Biol Chem. 2016;291:16541-52 pubmed publisher
  • flow cytometry; human; fig s6b
Srivastava P, Paluch B, Matsuzaki J, James S, Collamat Lai G, Blagitko Dorfs N, et al. Induction of cancer testis antigen expression in circulating acute myeloid leukemia blasts following hypomethylating agent monotherapy. Oncotarget. 2016;7:12840-56 pubmed publisher
  • flow cytometry; human; fig 6
Rancan C, Schirrmann L, Hüls C, Zeidler R, Moosmann A. Latent Membrane Protein LMP2A Impairs Recognition of EBV-Infected Cells by CD8+ T Cells. PLoS Pathog. 2015;11:e1004906 pubmed publisher
  • flow cytometry; equine; loading ...; fig 4
Meulenbroeks C, van der Lugt J, van der Meide N, Willemse T, Rutten V, Zaiss D. Allergen-Specific Cytokine Polarization Protects Shetland Ponies against Culicoides obsoletus-Induced Insect Bite Hypersensitivity. PLoS ONE. 2015;10:e0122090 pubmed publisher
  • flow cytometry; human; fig 3
Lee J, Breton G, Oliveira T, Zhou Y, Aljoufi A, PUHR S, et al. Restricted dendritic cell and monocyte progenitors in human cord blood and bone marrow. J Exp Med. 2015;212:385-99 pubmed publisher
  • flow cytometry; human; fig 7
Phadnis Moghe A, Crawford R, Kaminski N. Suppression of human B cell activation by 2,3,7,8-tetrachlorodibenzo-p-dioxin involves altered regulation of B cell lymphoma-6. Toxicol Sci. 2015;144:39-50 pubmed publisher
  • flow cytometry; mouse
  • flow cytometry; human
Cousens L, Najafian N, Martin W, De Groot A. Tregitope: Immunomodulation powerhouse. Hum Immunol. 2014;75:1139-46 pubmed publisher
  • flow cytometry; human; fig 1
Boltjes A, van Montfoort N, Biesta P, Op den Brouw M, Kwekkeboom J, van der Laan L, et al. Kupffer cells interact with hepatitis B surface antigen in vivo and in vitro, leading to proinflammatory cytokine production and natural killer cell function. J Infect Dis. 2015;211:1268-78 pubmed publisher
  • flow cytometry; human; fig 4
Brummelman J, Veerman R, Hamstra H, Deuss A, Schuijt T, Sloots A, et al. Bordetella pertussis naturally occurring isolates with altered lipooligosaccharide structure fail to fully mature human dendritic cells. Infect Immun. 2015;83:227-38 pubmed publisher
  • flow cytometry; human
Ziblat A, Domaica C, Spallanzani R, Iraolagoitia X, Rossi L, Avila D, et al. IL-27 stimulates human NK-cell effector functions and primes NK cells for IL-18 responsiveness. Eur J Immunol. 2015;45:192-202 pubmed publisher
  • flow cytometry; human
Spaan M, Kreefft K, de Graav G, Brouwer W, de Knegt R, ten Kate F, et al. CD4+ CXCR5+ T cells in chronic HCV infection produce less IL-21, yet are efficient at supporting B cell responses. J Hepatol. 2015;62:303-10 pubmed publisher
  • blocking or activating experiments; human; 10 ug/ml
Preciado Llanes L, Wing J, Foster R, Carlring J, Lees A, Read R, et al. Contact dependent suppression of CD4 T cell activation and proliferation by B cells activated through IgD cross-linking. Immunology. 2014;: pubmed publisher
  • flow cytometry; human
Chao Y, Kaliaperumal N, Chretien A, Tang S, Lee B, Poidinger M, et al. Human plasmacytoid dendritic cells regulate IFN-α production through activation-induced splicing of IL-18Rα. J Leukoc Biol. 2014;96:1037-46 pubmed publisher
  • flow cytometry; human
Royle C, Graham D, Sharma S, Fuchs D, Boasso A. HIV-1 and HIV-2 differentially mature plasmacytoid dendritic cells into IFN-producing cells or APCs. J Immunol. 2014;193:3538-48 pubmed publisher
  • flow cytometry; human
Lee Chang C, Bodogai M, Moritoh K, Olkhanud P, Chan A, Croft M, et al. Accumulation of 4-1BBL+ B cells in the elderly induces the generation of granzyme-B+ CD8+ T cells with potential antitumor activity. Blood. 2014;124:1450-9 pubmed publisher
  • flow cytometry; mouse
Skrnjug I, Rueckert C, Libanova R, Lienenklaus S, Weiss S, Guzman C. The mucosal adjuvant cyclic di-AMP exerts immune stimulatory effects on dendritic cells and macrophages. PLoS ONE. 2014;9:e95728 pubmed publisher
  • flow cytometry; human; loading ...; fig 4d
Duggal N, Beswetherick A, Upton J, Hampson P, Phillips A, Lord J. Depressive symptoms in hip fracture patients are associated with reduced monocyte superoxide production. Exp Gerontol. 2014;54:27-34 pubmed publisher
  • flow cytometry; human; loading ...; fig 2a
Svajger U, Obermajer N, Jeras M. IFN-?-rich environment programs dendritic cells toward silencing of cytotoxic immune responses. J Leukoc Biol. 2014;95:33-46 pubmed publisher
Laszlo G, Gudgeon C, Harrington K, Walter R. T-cell ligands modulate the cytolytic activity of the CD33/CD3 BiTE antibody construct, AMG 330. Blood Cancer J. 2015;5:e340 pubmed publisher
product information
Antigen :
CD86
Apps. Abbrev. :
Flow Cytometry, IHC-F, Western Blotting
Cat # :
305410
Clone :
IT2.2
Item :
LEAF™ Purified anti-human CD86
Isotype :
Mouse IgG2b, κ
Other Names :
B7-2, B70
Size :
500 μg
Price (USD) :
320 USD
Reactivity :
Human, African Green, Baboon, Capuchin Monkey, Common Marmoset, Cotton-topped Tamarin, Chimpanzee, Cynomolgus, Rhesus
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
LEAF
Application Notes :
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections 6 , Western blotting 3 , and blocking of T cell activation 2,4,5 . The Ultra-LEAF™ purified antibody (Endotoxin 0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. Nos. 305449 305450).
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA