product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Purified anti-human CD80
catalog :
305202
quantity :
100 μg
price :
94 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
2D10
reactivity :
human, mouse
application :
western blot, immunoprecipitation, flow cytometry, immunohistochemistry - frozen section, mass cytometry
more info or order :
citations: 32
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; loading ...; fig 1b
Piao W, Li L, Saxena V, Iyyathurai J, Lakhan R, Zhang Y, et al. PD-L1 signaling selectively regulates T cell lymphatic transendothelial migration. Nat Commun. 2022;13:2176 pubmed publisher
  • flow cytometry; human; loading ...; fig 3s2b
Gregorova M, Morse D, Brignoli T, Steventon J, Hamilton F, Albur M, et al. Post-acute COVID-19 associated with evidence of bystander T-cell activation and a recurring antibiotic-resistant bacterial pneumonia. elife. 2020;9: pubmed publisher
  • flow cytometry; human; loading ...; fig s1c
Morrissey M, Byrne R, Nulty C, McCabe N, Lynam Lennon N, Butler C, et al. The tumour microenvironment of the upper and lower gastrointestinal tract differentially influences dendritic cell maturation. BMC Cancer. 2020;20:566 pubmed publisher
  • mass cytometry; human; 500 ug/ml; loading ...; fig s11a
Helmink B, Reddy S, Gao J, Zhang S, Basar R, Thakur R, et al. B cells and tertiary lymphoid structures promote immunotherapy response. Nature. 2020;577:549-555 pubmed publisher
  • flow cytometry; human; 1 ug/ml; loading ...; fig s3a
Le Nours J, Gherardin N, Ramarathinam S, Awad W, Wiede F, Gully B, et al. A class of γδ T cell receptors recognize the underside of the antigen-presenting molecule MR1. Science. 2019;366:1522-1527 pubmed publisher
  • flow cytometry; human; fig 3b
Humeniuk P, Geiselhart S, Battin C, Webb T, Steinberger P, Paster W, et al. Generation of a Jurkat-based fluorescent reporter cell line to evaluate lipid antigen interaction with the human iNKT cell receptor. Sci Rep. 2019;9:7426 pubmed publisher
  • flow cytometry; human; loading ...; fig 4e
Hammerich L, Marron T, Upadhyay R, Svensson Arvelund J, Dhainaut M, Hussein S, et al. Systemic clinical tumor regressions and potentiation of PD1 blockade with in situ vaccination. Nat Med. 2019;25:814-824 pubmed publisher
  • flow cytometry; human; loading ...; fig 1b
Tremblay McLean A, Coenraads S, Kiani Z, Dupuy F, Bernard N. Expression of ligands for activating natural killer cell receptors on cell lines commonly used to assess natural killer cell function. BMC Immunol. 2019;20:8 pubmed publisher
  • flow cytometry; human; loading ...; fig 1b
Richardson J, Armbruster N, Günter M, Henes J, Autenrieth S. Staphylococcus aureus PSM Peptides Modulate Human Monocyte-Derived Dendritic Cells to Prime Regulatory T Cells. Front Immunol. 2018;9:2603 pubmed publisher
  • flow cytometry; human; loading ...; fig s1b
Redka D, Gutschow M, Grinstein S, Canton J. Differential ability of proinflammatory and anti-inflammatory macrophages to perform macropinocytosis. Mol Biol Cell. 2018;29:53-65 pubmed publisher
  • flow cytometry; human; loading ...; fig 2a
Salio M, Gasser O, González López C, Martens A, Veerapen N, Gileadi U, et al. Activation of Human Mucosal-Associated Invariant T Cells Induces CD40L-Dependent Maturation of Monocyte-Derived and Primary Dendritic Cells. J Immunol. 2017;199:2631-2638 pubmed publisher
  • flow cytometry; human; 1:50; loading ...; fig s1d
Smith N, Pietrancosta N, Davidson S, Dutrieux J, Chauveau L, Cutolo P, et al. Natural amines inhibit activation of human plasmacytoid dendritic cells through CXCR4 engagement. Nat Commun. 2017;8:14253 pubmed publisher
  • flow cytometry; human; tbl 3
Fromm J, Thomas A, Wood B. Characterization and Purification of Neoplastic Cells of Nodular Lymphocyte Predominant Hodgkin Lymphoma from Lymph Nodes by Flow Cytometry and Flow Cytometric Cell Sorting. Am J Pathol. 2017;187:304-317 pubmed publisher
  • flow cytometry; human; fig s6b
Srivastava P, Paluch B, Matsuzaki J, James S, Collamat Lai G, Blagitko Dorfs N, et al. Induction of cancer testis antigen expression in circulating acute myeloid leukemia blasts following hypomethylating agent monotherapy. Oncotarget. 2016;7:12840-56 pubmed publisher
  • flow cytometry; human; fig 1
Jutz S, Leitner J, Schmetterer K, Doel Perez I, Majdic O, Grabmeier Pfistershammer K, et al. Assessment of costimulation and coinhibition in a triple parameter T cell reporter line: Simultaneous measurement of NF-κB, NFAT and AP-1. J Immunol Methods. 2016;430:10-20 pubmed publisher
  • flow cytometry; human; loading ...; fig 1b
Trabanelli S, Lecciso M, Salvestrini V, Cavo M, Očadlíková D, Lemoli R, et al. PGE2-induced IDO1 inhibits the capacity of fully mature DCs to elicit an in vitro antileukemic immune response. J Immunol Res. 2015;2015:253191 pubmed publisher
  • flow cytometry; human; fig 3
Lee J, Breton G, Oliveira T, Zhou Y, Aljoufi A, PUHR S, et al. Restricted dendritic cell and monocyte progenitors in human cord blood and bone marrow. J Exp Med. 2015;212:385-99 pubmed publisher
  • flow cytometry; human; fig 4
Phadnis Moghe A, Crawford R, Kaminski N. Suppression of human B cell activation by 2,3,7,8-tetrachlorodibenzo-p-dioxin involves altered regulation of B cell lymphoma-6. Toxicol Sci. 2015;144:39-50 pubmed publisher
  • flow cytometry; human
Škrnjug I, Guzmán C, Rueckert C, Ruecker C. Cyclic GMP-AMP displays mucosal adjuvant activity in mice. PLoS ONE. 2014;9:e110150 pubmed publisher
  • flow cytometry; human
Lee Chang C, Bodogai M, Moritoh K, Olkhanud P, Chan A, Croft M, et al. Accumulation of 4-1BBL+ B cells in the elderly induces the generation of granzyme-B+ CD8+ T cells with potential antitumor activity. Blood. 2014;124:1450-9 pubmed publisher
  • flow cytometry; mouse
Skrnjug I, Rueckert C, Libanova R, Lienenklaus S, Weiss S, Guzman C. The mucosal adjuvant cyclic di-AMP exerts immune stimulatory effects on dendritic cells and macrophages. PLoS ONE. 2014;9:e95728 pubmed publisher
  • flow cytometry; human; loading ...; fig 4b
Duggal N, Beswetherick A, Upton J, Hampson P, Phillips A, Lord J. Depressive symptoms in hip fracture patients are associated with reduced monocyte superoxide production. Exp Gerontol. 2014;54:27-34 pubmed publisher
  • flow cytometry; human; loading ...; fig 2a
Svajger U, Obermajer N, Jeras M. IFN-?-rich environment programs dendritic cells toward silencing of cytotoxic immune responses. J Leukoc Biol. 2014;95:33-46 pubmed publisher
Azari S, Johnson L, Webb A, Kozlowski S, Zhang X, Rood K, et al. Hofbauer Cells Spread Listeria monocytogenes among Placental Cells and Undergo Pro-Inflammatory Reprogramming while Retaining Production of Tolerogenic Factors. MBio. 2021;12:e0184921 pubmed publisher
Roussel M, Ferrant J, Reizine F, Le Gallou S, Dulong J, Carl S, et al. Comparative immune profiling of acute respiratory distress syndrome patients with or without SARS-CoV-2 infection. Cell Rep Med. 2021;2:100291 pubmed publisher
Hu G, Su Y, Kang B, Fan Z, Dong T, Brown D, et al. High-throughput phenotypic screen and transcriptional analysis identify new compounds and targets for macrophage reprogramming. Nat Commun. 2021;12:773 pubmed publisher
Parker R, Partridge T, Wormald C, Kawahara R, Stalls V, Aggelakopoulou M, et al. Mapping the SARS-CoV-2 spike glycoprotein-derived peptidome presented by HLA class II on dendritic cells. bioRxiv. 2020;: pubmed publisher
Zimmerman M, Quicke K, O Neal J, Arora N, Machiah D, Priyamvada L, et al. Cross-Reactive Dengue Virus Antibodies Augment Zika Virus Infection of Human Placental Macrophages. Cell Host Microbe. 2018;24:731-742.e6 pubmed publisher
Herrera M, Söderberg M, Sabirsh A, Valastro B, Mölne J, Santamaria B, et al. Inhibition of T-cell activation by the CTLA4-Fc Abatacept is sufficient to ameliorate proteinuric kidney disease. Am J Physiol Renal Physiol. 2017;312:F748-F759 pubmed publisher
Wen T, Mingler M, Wahl B, Khorki M, Pabst O, Zimmermann N, et al. Carbonic anhydrase IV is expressed on IL-5-activated murine eosinophils. J Immunol. 2014;192:5481-9 pubmed publisher
Schubert D, Gordo S, Sabatino J, Vardhana S, Gagnon E, Sethi D, et al. Self-reactive human CD4 T cell clones form unusual immunological synapses. J Exp Med. 2012;209:335-52 pubmed publisher
Jayakumar A, Donovan M, Tripathi V, Ramalho Ortigao M, McDowell M. Leishmania major infection activates NF-kappaB and interferon regulatory factors 1 and 8 in human dendritic cells. Infect Immun. 2008;76:2138-48 pubmed publisher
product information
Antigen :
CD80
Apps. Abbrev. :
FC, WB, IP, IHC-F
Cat # :
305202
Clone :
2D10
Item :
Purified anti-human CD80
Isotype :
Mouse IgG1, κ
Other Names :
B7-1, B7, BB1
Size :
100 μg
Price (USD) :
94 USD
Reactivity :
Human
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
PURE
Application Notes :
Additional reported applications (for the relevant formats) include: in vitro blocking of T cell activation, immunohistochemical staining of acetone-fixed frozen tissue sections^2, immunoprecipitation, and Western blotting^3. The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 305245 & 305246).;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA