product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Purified anti-human CD64
catalog :
305002
quantity :
100 μg
price :
102 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
10.1
reactivity :
human
application :
neutralization, flow cytometry, immunohistochemistry - frozen section, blocking or activating experiments, mass cytometry
more info or order :
citations: 34
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; loading ...; fig 7a, 7c, 7e
Rasoulouniriana D, Santana Magal N, Gutwillig A, Farhat Younis L, Wine Y, Saperia C, et al. A distinct subset of FcγRI-expressing Th1 cells exert antibody-mediated cytotoxic activity. J Clin Invest. 2019;129:4151-4164 pubmed publisher
  • mass cytometry; human; loading ...; fig 2j
Wagner J, Rapsomaniki M, Chevrier S, Anzeneder T, Langwieder C, Dykgers A, et al. A Single-Cell Atlas of the Tumor and Immune Ecosystem of Human Breast Cancer. Cell. 2019;177:1330-1345.e18 pubmed publisher
  • flow cytometry; human; 1:200; loading ...; fig 1c
Jones G, Bain C, Fenton T, Kelly A, Brown S, Ivens A, et al. Dynamics of Colon Monocyte and Macrophage Activation During Colitis. Front Immunol. 2018;9:2764 pubmed publisher
  • flow cytometry; human; loading ...; fig 1a
Olin A, Henckel E, Chen Y, Lakshmikanth T, Pou C, Mikes J, et al. Stereotypic Immune System Development in Newborn Children. Cell. 2018;174:1277-1292.e14 pubmed publisher
  • flow cytometry; human; loading ...; fig 1e
Bujko A, Atlasy N, Landsverk O, Richter L, Yaqub S, Horneland R, et al. Transcriptional and functional profiling defines human small intestinal macrophage subsets. J Exp Med. 2018;215:441-458 pubmed publisher
  • flow cytometry; human; loading ...; fig 4a
Hally K, La Flamme A, Larsen P, Harding S. Platelet Toll-like receptor (TLR) expression and TLR-mediated platelet activation in acute myocardial infarction. Thromb Res. 2017;158:8-15 pubmed publisher
  • flow cytometry; human; loading ...; fig st1
Gordon S, Maute R, Dulken B, Hutter G, George B, McCracken M, et al. PD-1 expression by tumour-associated macrophages inhibits phagocytosis and tumour immunity. Nature. 2017;545:495-499 pubmed publisher
  • blocking or activating experiments; human; loading ...; fig 3a
  • flow cytometry; human; loading ...; fig 3
Yeap W, Wong K, Shimasaki N, Teo E, Quek J, Yong H, et al. CD16 is indispensable for antibody-dependent cellular cytotoxicity by human monocytes. Sci Rep. 2016;6:34310 pubmed publisher
  • flow cytometry; human
Al Barwani F, Young S, Baird M, Larsen D, Ward V. Mannosylation of virus-like particles enhances internalization by antigen presenting cells. PLoS ONE. 2014;9:e104523 pubmed publisher
  • flow cytometry; human
Vogel K, Thomann S, Vogel B, Schuster P, Schmidt B. Both plasmacytoid dendritic cells and monocytes stimulate natural killer cells early during human herpes simplex virus type 1 infections. Immunology. 2014;143:588-600 pubmed publisher
Vivanco Gonzalez N, Oliveria J, Tebaykin D, Ivison G, Mukai K, Tsai M, et al. An optimized protocol for phenotyping human granulocytes by mass cytometry. STAR Protoc. 2022;3:101280 pubmed publisher
Alpert A, Nahman O, Starosvetsky E, Hayun M, Curiel T, Ofran Y, et al. Alignment of single-cell trajectories by tuMap enables high-resolution quantitative comparison of cancer samples. Cell Syst. 2022;13:71-82.e8 pubmed publisher
Henrick B, Rodriguez L, Lakshmikanth T, Pou C, Henckel E, Arzoomand A, et al. Bifidobacteria-mediated immune system imprinting early in life. Cell. 2021;184:3884-3898.e11 pubmed publisher
Gonz xe1 lez L, Melo Gonz xe1 lez F, Sebasti xe1 n V, Vallejos O, Noguera L, Suazo I, et al. Characterization of the Anti-Inflammatory Capacity of IL-10-Producing Neutrophils in Response to Streptococcus pneumoniae Infection. Front Immunol. 2021;12:638917 pubmed publisher
Keck S, Galati Fournier V, Kym U, Moesch M, Usemann J, Muller I, et al. Lack of Mucosal Cholinergic Innervation Is Associated With Increased Risk of Enterocolitis in Hirschsprung's Disease. Cell Mol Gastroenterol Hepatol. 2021;12:507-545 pubmed publisher
Chevrier S, Zurbuchen Y, Cervia C, Adamo S, Raeber M, de Souza N, et al. A distinct innate immune signature marks progression from mild to severe COVID-19. Cell Rep Med. 2021;2:100166 pubmed publisher
Combes A, Courau T, Kuhn N, Hu K, Ray A, Chen W, et al. Global absence and targeting of protective immune states in severe COVID-19. Nature. 2021;591:124-130 pubmed publisher
Mann E, Menon M, Knight S, Konkel J, Jagger C, Shaw T, et al. Longitudinal immune profiling reveals key myeloid signatures associated with COVID-19. Sci Immunol. 2020;5: pubmed publisher
Rodriguez L, Pekkarinen P, Lakshmikanth T, Tan Z, Consiglio C, Pou C, et al. Systems-Level Immunomonitoring from Acute to Recovery Phase of Severe COVID-19. Cell Rep Med. 2020;1:100078 pubmed publisher
Weinhage T, K xf6 lsche T, Rieger Fackeldey E, Schmitz R, Antoni A, Ahlmann M, et al. Cord Blood Low-Density Granulocytes Correspond to an Immature Granulocytic Subset with Low Expression of S100A12. J Immunol. 2020;205:56-66 pubmed publisher
Steffen U, Koeleman C, Sokolova M, Bang H, Kleyer A, Rech J, et al. IgA subclasses have different effector functions associated with distinct glycosylation profiles. Nat Commun. 2020;11:120 pubmed publisher
Keskin D, Anandappa A, Sun J, Tirosh I, Mathewson N, Li S, et al. Neoantigen vaccine generates intratumoral T cell responses in phase Ib glioblastoma trial. Nature. 2019;565:234-239 pubmed publisher
Jog N, Chakravarty E, Guthridge J, James J. Epstein Barr Virus Interleukin 10 Suppresses Anti-inflammatory Phenotype in Human Monocytes. Front Immunol. 2018;9:2198 pubmed publisher
Elias S, Kahlon S, Kotzur R, Kaynan N, Mandelboim O. Obinutuzumab activates FcγRI more potently than other anti-CD20 antibodies in chronic lymphocytic leukemia (CLL). Oncoimmunology. 2018;7:e1428158 pubmed publisher
Vermi W, Micheletti A, Finotti G, Tecchio C, Calzetti F, Costa S, et al. slan+ Monocytes and Macrophages Mediate CD20-Dependent B-cell Lymphoma Elimination via ADCC and ADCP. Cancer Res. 2018;78:3544-3559 pubmed publisher
Zhong Q, Gong F, Gong Z, Hua S, Zeng K, Gao X. IgG Immunocomplexes Sensitize Human Monocytes for Inflammatory Hyperactivity via Transcriptomic and Epigenetic Reprogramming in Rheumatoid Arthritis. J Immunol. 2018;200:3913-3925 pubmed publisher
Chevrier S, Crowell H, Zanotelli V, Engler S, Robinson M, Bodenmiller B. Compensation of Signal Spillover in Suspension and Imaging Mass Cytometry. Cell Syst. 2018;6:612-620.e5 pubmed publisher
Evrard M, Kwok I, Chong S, Teng K, Becht E, Chen J, et al. Developmental Analysis of Bone Marrow Neutrophils Reveals Populations Specialized in Expansion, Trafficking, and Effector Functions. Immunity. 2018;48:364-379.e8 pubmed publisher
Chen W, Pilling D, Gomer R. C-reactive protein (CRP) but not the related pentraxins serum amyloid P and PTX3 inhibits the proliferation and induces apoptosis of the leukemia cell line Mono Mac 6. BMC Immunol. 2017;18:47 pubmed publisher
Prodjinotho U, von Horn C, Debrah A, Batsa Debrah L, Albers A, Layland L, et al. Pathological manifestations in lymphatic filariasis correlate with lack of inhibitory properties of IgG4 antibodies on IgE-activated granulocytes. PLoS Negl Trop Dis. 2017;11:e0005777 pubmed publisher
Chudnovskiy A, Mortha A, Kana V, Kennard A, Ramírez J, Rahman A, et al. Host-Protozoan Interactions Protect from Mucosal Infections through Activation of the Inflammasome. Cell. 2016;167:444-456.e14 pubmed publisher
Bruhns P, Iannascoli B, England P, Mancardi D, Fernandez N, Jorieux S, et al. Specificity and affinity of human Fcgamma receptors and their polymorphic variants for human IgG subclasses. Blood. 2009;113:3716-25 pubmed publisher
Cho H, Shashkin P, Gleissner C, Dunson D, Jain N, Lee J, et al. Induction of dendritic cell-like phenotype in macrophages during foam cell formation. Physiol Genomics. 2007;29:149-60 pubmed
Van Tits L, de Graaf J, Toenhake H, van Heerde W, Stalenhoef A. C-reactive protein and annexin A5 bind to distinct sites of negatively charged phospholipids present in oxidized low-density lipoprotein. Arterioscler Thromb Vasc Biol. 2005;25:717-22 pubmed
product information
Antigen :
CD64
Apps. Abbrev. :
FC, IHC-F, Block
Cat # :
305002
Clone :
10.1
Item :
Purified anti-human CD64
Isotype :
Mouse IgG1, κ
Other Names :
FcγRI, FcR I, FCGR1A
Size :
100 μg
Price (USD) :
102 USD
Reactivity :
Human, Cynomolgus, Rhesus
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
PURE
Immunogen :
Human rheumatoid synovial fluid cells and fibronectin-purified monocytes.
Application Notes :
Clone 10.1 recognizes the EC3 epitope of CD64. While both contain the EC3 domain, in-house testing suggests that clone 10.1 preferentially binds to CD64A (FcγRIA), but not CD64B (FcγRIB). Additional reported applications (for the relevant formats) include: blocking of human IgG3 and murine IgG2a binding to FcγRI^2,5,6,11 and immunohistochemical staining of acetone-fixed frozen tissue sections^12.;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA