product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Alexa Fluor® 594 anti-human CD45
catalog :
304060
quantity :
100 μg
price :
225 USD
clonality :
monoclonal
host :
mouse
conjugate :
Alexa Fluor 594
clone name :
HI30
reactivity :
African green monkey, human, mouse
application :
immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, blocking or activating experiments, mass cytometry
more info or order :
citations: 44
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; loading ...; fig s1
Katano I, Ito R, Kawai K, Takahashi T. Improved Detection of in vivo Human NK Cell-Mediated Antibody-Dependent Cellular Cytotoxicity Using a Novel NOG-FcγR-Deficient Human IL-15 Transgenic Mouse. Front Immunol. 2020;11:532684 pubmed publisher
  • immunocytochemistry; human; 10 ug/ml; loading ...; fig 2b
Ortiz Otero N, Marshall J, Lash B, King M. Chemotherapy-induced release of circulating-tumor cells into the bloodstream in collective migration units with cancer-associated fibroblasts in metastatic cancer patients. BMC Cancer. 2020;20:873 pubmed publisher
  • flow cytometry; human; loading ...; fig 1b
Stary V, Wolf B, Unterleuthner D, List J, Talic M, Laengle J, et al. Short-course radiotherapy promotes pro-inflammatory macrophages via extracellular vesicles in human rectal cancer. J Immunother Cancer. 2020;8: pubmed publisher
  • immunohistochemistry - frozen section; mouse; loading ...; fig 9a
Kaaij M, van Tok M, Blijdorp I, Ambarus C, Stock M, Pots D, et al. Transmembrane TNF drives osteoproliferative joint inflammation reminiscent of human spondyloarthritis. J Exp Med. 2020;217: pubmed publisher
  • flow cytometry; human; loading ...
Wong S, Lenzini S, Cooper M, Mooney D, Shin J. Soft extracellular matrix enhances inflammatory activation of mesenchymal stromal cells to induce monocyte production and trafficking. Sci Adv. 2020;6:eaaw0158 pubmed publisher
  • mass cytometry; human; loading ...; fig 1b, 1d, s2
Leylek R, Alcántara Hernández M, Lanzar Z, Lüdtke A, Perez O, Reizis B, et al. Integrated Cross-Species Analysis Identifies a Conserved Transitional Dendritic Cell Population. Cell Rep. 2019;29:3736-3750.e8 pubmed publisher
  • immunocytochemistry; human; 1:250; fig 2a
Bokun V, Moore J, Moore R, Smallcombe C, Harford T, Rezaee F, et al. Respiratory syncytial virus exhibits differential tropism for distinct human placental cell types with Hofbauer cells acting as a permissive reservoir for infection. PLoS ONE. 2019;14:e0225767 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s19b
  • flow cytometry; human; loading ...; fig s14b
Stewart B, Ferdinand J, Young M, Mitchell T, Loudon K, Riding A, et al. Spatiotemporal immune zonation of the human kidney. Science. 2019;365:1461-1466 pubmed publisher
  • flow cytometry; human; loading ...; fig 1a
Allden S, Ogger P, Ghai P, McErlean P, Hewitt R, Toshner R, et al. The Transferrin Receptor CD71 Delineates Functionally Distinct Airway Macrophage Subsets during Idiopathic Pulmonary Fibrosis. Am J Respir Crit Care Med. 2019;: pubmed publisher
  • mass cytometry; human; loading ...; fig s1
Fernandez I, Baxter R, Garcia Perez J, Vendrame E, Ranganath T, Kong D, et al. A novel human IL2RB mutation results in T and NK cell-driven immune dysregulation. J Exp Med. 2019;216:1255-1267 pubmed publisher
  • flow cytometry; human; loading ...; fig s3
Lim S, Kim J, Jeon S, Shin M, Kwon J, Kim T, et al. Defective Localization With Impaired Tumor Cytotoxicity Contributes to the Immune Escape of NK Cells in Pancreatic Cancer Patients. Front Immunol. 2019;10:496 pubmed publisher
  • flow cytometry; human; fig 1c
Montel Hagen A, Seet C, Li S, Chick B, Zhu Y, Chang P, et al. Organoid-Induced Differentiation of Conventional T Cells from Human Pluripotent Stem Cells. Cell Stem Cell. 2019;24:376-389.e8 pubmed publisher
  • flow cytometry; human; loading ...; tbl s1
Dias J, Boulouis C, Gorin J, van den Biggelaar R, Lal K, Gibbs A, et al. The CD4-CD8- MAIT cell subpopulation is a functionally distinct subset developmentally related to the main CD8+ MAIT cell pool. Proc Natl Acad Sci U S A. 2018;115:E11513-E11522 pubmed publisher
  • flow cytometry; human; loading ...; fig s3b
Arnold I, Artola Borán M, Tallón de Lara P, Kyburz A, Taube C, OTTEMANN K, et al. Eosinophils suppress Th1 responses and restrict bacterially induced gastrointestinal inflammation. J Exp Med. 2018;215:2055-2072 pubmed publisher
  • flow cytometry; human; loading ...; fig 9b
Casey A, Sinha A, Singhania R, Livingstone J, Waterhouse P, Tharmapalan P, et al. Mammary molecular portraits reveal lineage-specific features and progenitor cell vulnerabilities. J Cell Biol. 2018;217:2951-2974 pubmed publisher
  • flow cytometry; human; fig 1a
Bujko A, Atlasy N, Landsverk O, Richter L, Yaqub S, Horneland R, et al. Transcriptional and functional profiling defines human small intestinal macrophage subsets. J Exp Med. 2018;215:441-458 pubmed publisher
  • flow cytometry; human; loading ...; fig 1c
Herndler Brandstetter D, Shan L, Yao Y, Stecher C, Plajer V, Lietzenmayer M, et al. Humanized mouse model supports development, function, and tissue residency of human natural killer cells. Proc Natl Acad Sci U S A. 2017;114:E9626-E9634 pubmed publisher
  • flow cytometry; human; loading ...; fig 6c
Daley D, Mani V, Mohan N, Akkad N, Ochi A, Heindel D, et al. Dectin 1 activation on macrophages by galectin 9 promotes pancreatic carcinoma and peritumoral immune tolerance. Nat Med. 2017;23:556-567 pubmed publisher
  • flow cytometry; human; 1:33; loading ...; fig 3a
Britschgi A, Duss S, Kim S, Couto J, Brinkhaus H, Koren S, et al. The Hippo kinases LATS1 and 2 control human breast cell fate via crosstalk with ERα. Nature. 2017;541:541-545 pubmed publisher
  • blocking or activating experiments; human; loading ...; fig s6c
Park R, Wang T, Koundakjian D, Hultquist J, Lamothe Molina P, Monel B, et al. A genome-wide CRISPR screen identifies a restricted set of HIV host dependency factors. Nat Genet. 2017;49:193-203 pubmed publisher
  • flow cytometry; human; loading ...; fig 1a
Wei C, Mei J, Tang L, Liu Y, Li D, Li M, et al. 1-Methyl-tryptophan attenuates regulatory T cells differentiation due to the inhibition of estrogen-IDO1-MRC2 axis in endometriosis. Cell Death Dis. 2016;7:e2489 pubmed publisher
  • flow cytometry; human; loading ...; fig 3a
Faivre V, Lukaszewicz A, Payen D. Downregulation of Blood Monocyte HLA-DR in ICU Patients Is Also Present in Bone Marrow Cells. PLoS ONE. 2016;11:e0164489 pubmed publisher
  • flow cytometry; human; loading ...; fig 3d
Ye C, Wang W, Cheng L, Li G, Wen M, Wang Q, et al. Glycosylphosphatidylinositol-Anchored Anti-HIV scFv Efficiently Protects CD4 T Cells from HIV-1 Infection and Deletion in hu-PBL Mice. J Virol. 2017;91: pubmed publisher
  • immunocytochemistry; human; loading ...; fig s7
Jung Y, Riven I, Feigelson S, Kartvelishvily E, Tohya K, Miyasaka M, et al. Three-dimensional localization of T-cell receptors in relation to microvilli using a combination of superresolution microscopies. Proc Natl Acad Sci U S A. 2016;113:E5916-E5924 pubmed
  • flow cytometry; human; 1:25; loading ...
Lu X, Chen Q, Rong Y, Yang G, Li C, Xu N, et al. LECT2 drives haematopoietic stem cell expansion and mobilization via regulating the macrophages and osteolineage cells. Nat Commun. 2016;7:12719 pubmed publisher
  • flow cytometry; human; loading ...; fig s1
Landtwing V, Raykova A, Pezzino G, Beziat V, Marcenaro E, Graf C, et al. Cognate HLA absence in trans diminishes human NK cell education. J Clin Invest. 2016;126:3772-3782 pubmed publisher
  • flow cytometry; human; loading ...
Cerny D, Thi Le D, The T, Zuest R, Kg S, Velumani S, et al. Complete human CD1a deficiency on Langerhans cells due to a rare point mutation in the coding sequence. J Allergy Clin Immunol. 2016;138:1709-1712.e11 pubmed publisher
  • immunocytochemistry; human; 1:150; fig s2
Hanssen A, Wagner J, Gorges T, Taenzer A, Uzunoglu F, Driemel C, et al. Characterization of different CTC subpopulations in non-small cell lung cancer. Sci Rep. 2016;6:28010 pubmed publisher
  • flow cytometry; African green monkey; loading ...; fig 2a
Neumann B, Shi T, Gan L, Klippert A, Daskalaki M, Stolte Leeb N, et al. Comprehensive panel of cross-reacting monoclonal antibodies for analysis of different immune cells and their distribution in the common marmoset (Callithrix jacchus). J Med Primatol. 2016;45:139-46 pubmed publisher
  • flow cytometry; human; loading ...; fig 1a
Bal S, Bernink J, Nagasawa M, Groot J, Shikhagaie M, Golebski K, et al. IL-1?, IL-4 and IL-12 control the fate of group 2 innate lymphoid cells in human airway inflammation in the lungs. Nat Immunol. 2016;17:636-45 pubmed publisher
  • flow cytometry; human; fig 4
Jackson J, Taylor J, Witek M, Hunsucker S, Waugh J, Fedoriw Y, et al. Microfluidics for the detection of minimal residual disease in acute myeloid leukemia patients using circulating leukemic cells selected from blood. Analyst. 2016;141:640-51 pubmed publisher
  • flow cytometry; human; loading ...; fig 1c
Bai H, Liu Y, Xie Y, Hoyle D, Brodsky R, Cheng L, et al. Definitive Hematopoietic Multipotent Progenitor Cells Are Transiently Generated From Hemogenic Endothelial Cells in Human Pluripotent Stem Cells. J Cell Physiol. 2016;231:1065-76 pubmed publisher
  • flow cytometry; human; fig 3
Berent Maoz B, Montecino Rodriguez E, Fice M, Casero D, Seet C, Crooks G, et al. The expansion of thymopoiesis in neonatal mice is dependent on expression of high mobility group a 2 protein (Hmga2). PLoS ONE. 2015;10:e0125414 pubmed publisher
  • flow cytometry; human; fig 1
Marquardt N, Béziat V, Nyström S, Hengst J, Ivarsson M, Kekäläinen E, et al. Cutting edge: identification and characterization of human intrahepatic CD49a+ NK cells. J Immunol. 2015;194:2467-71 pubmed publisher
  • flow cytometry; human; fig 2
Du Z, Abedalthagafi M, Aizer A, McHenry A, Sun H, Bray M, et al. Increased expression of the immune modulatory molecule PD-L1 (CD274) in anaplastic meningioma. Oncotarget. 2015;6:4704-16 pubmed
  • flow cytometry; human; 2.5 mg/ml; fig 3
Zhang P, Lu X, Tao K, Shi L, Li W, Wang G, et al. Siglec-10 is associated with survival and natural killer cell dysfunction in hepatocellular carcinoma. J Surg Res. 2015;194:107-13 pubmed publisher
Chou D, Frismantas V, Milton Y, David R, Pop Damkov P, Ferguson D, et al. On-chip recapitulation of clinical bone marrow toxicities and patient-specific pathophysiology. Nat Biomed Eng. 2020;4:394-406 pubmed publisher
Hollern D, Xu N, Thennavan A, Glodowski C, García Recio S, Mott K, et al. B Cells and T Follicular Helper Cells Mediate Response to Checkpoint Inhibitors in High Mutation Burden Mouse Models of Breast Cancer. Cell. 2019;179:1191-1206.e21 pubmed publisher
Lee J, Breton G, Oliveira T, Zhou Y, Aljoufi A, PUHR S, et al. Restricted dendritic cell and monocyte progenitors in human cord blood and bone marrow. J Exp Med. 2015;212:385-99 pubmed publisher
Breton G, Lee J, Zhou Y, Schreiber J, Keler T, PUHR S, et al. Circulating precursors of human CD1c+ and CD141+ dendritic cells. J Exp Med. 2015;212:401-13 pubmed publisher
Bushway M, Gerber S, Fenton B, Miller R, Lord E, Murphy S. Morphological and phenotypic analyses of the human placenta using whole mount immunofluorescence. Biol Reprod. 2014;90:110 pubmed publisher
Oeztuerk Winder F, Guinot A, Ochalek A, Ventura J. Regulation of human lung alveolar multipotent cells by a novel p38α MAPK/miR-17-92 axis. EMBO J. 2012;31:3431-41 pubmed publisher
Morozov A, Downey R, Healey J, Moreira A, Lou E, Franceschino A, et al. Benign mesenchymal stromal cells in human sarcomas. Clin Cancer Res. 2010;16:5630-40 pubmed publisher
Jiang Q, Zhang L, Wang R, Jeffrey J, Washburn M, Brouwer D, et al. FoxP3+CD4+ regulatory T cells play an important role in acute HIV-1 infection in humanized Rag2-/-gammaC-/- mice in vivo. Blood. 2008;112:2858-68 pubmed publisher
product information
Antigen :
CD45
Apps. Abbrev. :
IHC-P
Cat # :
304060
Clone :
HI30
Item :
Alexa Fluor® 594 anti-human CD45
Isotype :
Mouse IgG1, κ
Other Names :
LCA, T200
Size :
100 μg
Price (USD) :
225 USD
Reactivity :
Human, Cross-Reactivity: Chimpanzee
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
A594
Application Notes :
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections 9 , inhibition of CD45 functions 4 , immunofluorescence 11 , and Western blotting 3 . It was found that the HI30 clone and the 2D1 clone can cross block each other s binding.
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA