product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Brilliant Violet 421™ anti-human CD45
catalog :
304031
quantity :
25 tests
price :
190 USD
clonality :
monoclonal
host :
mouse
conjugate :
BV421
clone name :
HI30
reactivity :
human
application :
flow cytometry
more info or order :
citations: 35
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; loading ...; fig 1f, s1b
Wu X, Xia T, Shin W, Yu K, Jung W, Herrmann A, et al. Viral Mimicry of Interleukin-17A by SARS-CoV-2 ORF8. MBio. 2022;13:e0040222 pubmed publisher
  • flow cytometry; human; loading ...; fig 5c
Rodriguez E, Boelaars K, Brown K, Eveline Li R, Kruijssen L, Bruijns S, et al. Sialic acids in pancreatic cancer cells drive tumour-associated macrophage differentiation via the Siglec receptors Siglec-7 and Siglec-9. Nat Commun. 2021;12:1270 pubmed publisher
  • flow cytometry; human; fig 3
Berent Maoz B, Montecino Rodriguez E, Fice M, Casero D, Seet C, Crooks G, et al. The expansion of thymopoiesis in neonatal mice is dependent on expression of high mobility group a 2 protein (Hmga2). PLoS ONE. 2015;10:e0125414 pubmed publisher
  • flow cytometry; human
Bendall S, Simonds E, Qiu P, Amir E, Krutzik P, Finck R, et al. Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum. Science. 2011;332:687-96 pubmed publisher
Synn C, Kim S, Lee H, Kim M, Kim J, Lee J, et al. SKI-G-801, an AXL kinase inhibitor, blocks metastasis through inducing anti-tumor immune responses and potentiates anti-PD-1 therapy in mouse cancer models. Clin Transl Immunology. 2022;11:e1364 pubmed publisher
Kim M, Cooper M, Jacobs M, Ritchey J, Hollaway J, Fehniger T, et al. CD7-deleted hematopoietic stem cells can restore immunity after CAR T cell therapy. JCI Insight. 2021;6: pubmed publisher
Maat H, Atsma T, Hogeling S, Rodríguez López A, Jaques J, Olthuis M, et al. The USP7-TRIM27 axis mediates non-canonical PRC1.1 function and is a druggable target in leukemia. iScience. 2021;24:102435 pubmed publisher
Pettmann J, Huhn A, Abu Shah E, Kutuzov M, Wilson D, Dustin M, et al. The discriminatory power of the T cell receptor. elife. 2021;10: pubmed publisher
Nafria M, Bonifer C, Stanley E, Ng E, Elefanty A. Protocol for the Generation of Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells. STAR Protoc. 2020;1:100130 pubmed publisher
Wang J, Xu Y, Chen Z, Liang J, Lin Z, Liang H, et al. Liver Immune Profiling Reveals Pathogenesis and Therapeutics for Biliary Atresia. Cell. 2020;183:1867-1883.e26 pubmed publisher
Bruveris F, Ng E, Leitoguinho A, Motazedian A, Vlahos K, Sourris K, et al. Human yolk sac-like haematopoiesis generates RUNX1-, GFI1- and/or GFI 1B-dependent blood and SOX17-positive endothelium. Development. 2020;147: pubmed publisher
Nafria M, Keane P, Ng E, Stanley E, Elefanty A, Bonifer C. Expression of RUNX1-ETO Rapidly Alters the Chromatin Landscape and Growth of Early Human Myeloid Precursor Cells. Cell Rep. 2020;31:107691 pubmed publisher
Worthen C, Cui Y, Orringer J, Johnson T, Voorhees J, Fisher G. CD26 Identifies a Subpopulation of Fibroblasts that Produce the Majority of Collagen during Wound Healing in Human Skin. J Invest Dermatol. 2020;140:2515-2524.e3 pubmed publisher
Eckert E, Nace R, Tonne J, Evgin L, Vile R, Russell S. Generation of a Tumor-Specific Chemokine Gradient Using Oncolytic Vesicular Stomatitis Virus Encoding CXCL9. Mol Ther Oncolytics. 2020;16:63-74 pubmed publisher
Hagan T, Cortese M, Rouphael N, Boudreau C, Linde C, Maddur M, et al. Antibiotics-Driven Gut Microbiome Perturbation Alters Immunity to Vaccines in Humans. Cell. 2019;178:1313-1328.e13 pubmed publisher
Pascual García M, Bonfill Teixidor E, Planas Rigol E, Rubio Perez C, Iurlaro R, Arias A, et al. LIF regulates CXCL9 in tumor-associated macrophages and prevents CD8+ T cell tumor-infiltration impairing anti-PD1 therapy. Nat Commun. 2019;10:2416 pubmed publisher
Xu L, Yuan N, Liu H, Fang Y, Ge C, Xu F, et al. Bafilomycin A1 targets patient-derived CD34CD19 leukemia stem cells. Haematologica. 2019;: pubmed publisher
Howden S, Hosseini Far H, Motazedian A, Elefanty A, Stanley E, Lamande S, et al. The use of simultaneous reprogramming and gene correction to generate an osteogenesis imperfecta patient COL1A1 c. 3936 G>T iPSC line and an isogenic control iPSC line. Stem Cell Res. 2019;38:101453 pubmed publisher
Hosseini Far H, Patria Y, Motazedian A, Elefanty A, Stanley E, Lamande S, et al. Generation of a heterozygous COL1A1 (c.3969_3970insT) osteogenesis imperfecta mutation human iPSC line, MCRIi001-A-1, using CRISPR/Cas9 editing. Stem Cell Res. 2019;37:101449 pubmed publisher
Jung J, Buisman S, Weersing E, Dethmers Ausema A, Zwart E, Schepers H, et al. CBX7 Induces Self-Renewal of Human Normal and Malignant Hematopoietic Stem and Progenitor Cells by Canonical and Non-canonical Interactions. Cell Rep. 2019;26:1906-1918.e8 pubmed publisher
Ruella M, Xu J, Barrett D, Fraietta J, Reich T, Ambrose D, et al. Induction of resistance to chimeric antigen receptor T cell therapy by transduction of a single leukemic B cell. Nat Med. 2018;24:1499-1503 pubmed publisher
Kim M, Yu K, Kenderian S, Ruella M, Chen S, Shin T, et al. Genetic Inactivation of CD33 in Hematopoietic Stem Cells to Enable CAR T Cell Immunotherapy for Acute Myeloid Leukemia. Cell. 2018;173:1439-1453.e19 pubmed publisher
Watanabe J, Togo S, Sumiyoshi I, Namba Y, Suina K, Mizuno T, et al. Clinical features of squamous cell lung cancer with anaplastic lymphoma kinase (ALK)-rearrangement: a retrospective analysis and review. Oncotarget. 2018;9:24000-24013 pubmed publisher
Hirota K, Hashimoto M, Ito Y, Matsuura M, Ito H, Tanaka M, et al. Autoimmune Th17 Cells Induced Synovial Stromal and Innate Lymphoid Cell Secretion of the Cytokine GM-CSF to Initiate and Augment Autoimmune Arthritis. Immunity. 2018;48:1220-1232.e5 pubmed publisher
Ng E, Azzola L, Bruveris F, Calvanese V, Phipson B, Vlahos K, et al. Differentiation of human embryonic stem cells to HOXA+ hemogenic vasculature that resembles the aorta-gonad-mesonephros. Nat Biotechnol. 2016;34:1168-1179 pubmed publisher
Polak R, de Rooij B, Pieters R, den Boer M. B-cell precursor acute lymphoblastic leukemia cells use tunneling nanotubes to orchestrate their microenvironment. Blood. 2015;126:2404-14 pubmed publisher
Ganti S, Albershardt T, Iritani B, Ruddell A. Regulatory B cells preferentially accumulate in tumor-draining lymph nodes and promote tumor growth. Sci Rep. 2015;5:12255 pubmed publisher
de Wit S, van Dalum G, Lenferink A, Tibbe A, Hiltermann T, Groen H, et al. The detection of EpCAM(+) and EpCAM(-) circulating tumor cells. Sci Rep. 2015;5:12270 pubmed publisher
Lee J, Breton G, Oliveira T, Zhou Y, Aljoufi A, PUHR S, et al. Restricted dendritic cell and monocyte progenitors in human cord blood and bone marrow. J Exp Med. 2015;212:385-99 pubmed publisher
Breton G, Lee J, Zhou Y, Schreiber J, Keler T, PUHR S, et al. Circulating precursors of human CD1c+ and CD141+ dendritic cells. J Exp Med. 2015;212:401-13 pubmed publisher
Marquardt N, Béziat V, Nyström S, Hengst J, Ivarsson M, Kekäläinen E, et al. Cutting edge: identification and characterization of human intrahepatic CD49a+ NK cells. J Immunol. 2015;194:2467-71 pubmed publisher
Bushway M, Gerber S, Fenton B, Miller R, Lord E, Murphy S. Morphological and phenotypic analyses of the human placenta using whole mount immunofluorescence. Biol Reprod. 2014;90:110 pubmed publisher
Oeztuerk Winder F, Guinot A, Ochalek A, Ventura J. Regulation of human lung alveolar multipotent cells by a novel p38α MAPK/miR-17-92 axis. EMBO J. 2012;31:3431-41 pubmed publisher
Morozov A, Downey R, Healey J, Moreira A, Lou E, Franceschino A, et al. Benign mesenchymal stromal cells in human sarcomas. Clin Cancer Res. 2010;16:5630-40 pubmed publisher
Jiang Q, Zhang L, Wang R, Jeffrey J, Washburn M, Brouwer D, et al. FoxP3+CD4+ regulatory T cells play an important role in acute HIV-1 infection in humanized Rag2-/-gammaC-/- mice in vivo. Blood. 2008;112:2858-68 pubmed publisher
product information
Antigen :
CD45
Apps. Abbrev. :
Flow Cytometry
Cat # :
304031
Clone :
HI30
Item :
Brilliant Violet 421™ anti-human CD45
Isotype :
Mouse IgG1, κ
Other Names :
LCA, T200
Size :
25 tests
Price (USD) :
190 USD
Reactivity :
Human
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
BV421
Application Notes :
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections 9 , inhibition of CD45 functions 4 , immunofluorescence 11 , Western blotting 3 , and spatial biology (IBEX) 16,17 . It was found that the HI30 clone and the 2D1 clone can cross block each other s binding.
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA