product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Pacific Blue™ anti-human CD45
catalog :
304022
quantity :
100 μg
price :
225 USD
clonality :
monoclonal
host :
mouse
conjugate :
Pacific Blue
clone name :
HI30
reactivity :
human, mouse
application :
flow cytometry
more info or order :
citations: 37
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; fig s1
Cheah M, Chen J, Sahoo D, Contreras Trujillo H, Volkmer A, Scheeren F, et al. CD14-expressing cancer cells establish the inflammatory and proliferative tumor microenvironment in bladder cancer. Proc Natl Acad Sci U S A. 2015;112:4725-30 pubmed publisher
  • flow cytometry; mouse; fig 8
Valle A, Barbagiovanni G, Jofra T, Stabilini A, Pérol L, Baeyens A, et al. Heterogeneous CD3 expression levels in differing T cell subsets correlate with the in vivo anti-CD3-mediated T cell modulation. J Immunol. 2015;194:2117-27 pubmed publisher
  • flow cytometry; human
Sumitomo S, Fujio K, Okamura T, Morita K, Ishigaki K, Suzukawa K, et al. Transcription factor early growth response 3 is associated with the TGF-?1 expression and the regulatory activity of CD4-positive T cells in vivo. J Immunol. 2013;191:2351-9 pubmed publisher
  • flow cytometry; human
Bendall S, Simonds E, Qiu P, Amir E, Krutzik P, Finck R, et al. Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum. Science. 2011;332:687-96 pubmed publisher
Zah E, Nam E, Bhuvan V, Tran U, Ji B, Gosliner S, et al. Systematically optimized BCMA/CS1 bispecific CAR-T cells robustly control heterogeneous multiple myeloma. Nat Commun. 2020;11:2283 pubmed publisher
Li S, Goncalves K, Lyu B, Yuan L, Hu G. Chemosensitization of prostate cancer stem cells in mice by angiogenin and plexin-B2 inhibitors. Commun Biol. 2020;3:26 pubmed publisher
Queckborner S, Syk Lundberg E, Gemzell Danielsson K, Davies L. Endometrial stromal cells exhibit a distinct phenotypic and immunomodulatory profile. Stem Cell Res Ther. 2020;11:15 pubmed publisher
Zhang Y, Du X, Liu M, Tang F, Zhang P, Ai C, et al. Hijacking antibody-induced CTLA-4 lysosomal degradation for safer and more effective cancer immunotherapy. Cell Res. 2019;29:609-627 pubmed publisher
Okumura T, Horie Y, Lai C, Lin H, Shoda H, Natsumoto B, et al. Robust and highly efficient hiPSC generation from patient non-mobilized peripheral blood-derived CD34+ cells using the auto-erasable Sendai virus vector. Stem Cell Res Ther. 2019;10:185 pubmed publisher
Beringer A, Molle J, Bartosch B, Miossec P. Two phase kinetics of the inflammatory response from hepatocyte-peripheral blood mononuclear cell interactions. Sci Rep. 2019;9:8378 pubmed publisher
Zimmerman K, Bentley M, Lever J, Li Z, Crossman D, Song C, et al. Single-Cell RNA Sequencing Identifies Candidate Renal Resident Macrophage Gene Expression Signatures across Species. J Am Soc Nephrol. 2019;30:767-781 pubmed publisher
Schiroli G, Conti A, Ferrari S, Della Volpe L, Jacob A, Albano L, et al. Precise Gene Editing Preserves Hematopoietic Stem Cell Function following Transient p53-Mediated DNA Damage Response. Cell Stem Cell. 2019;24:551-565.e8 pubmed publisher
Carter B, Mak P, Wang X, Tao W, Ruvolo V, Mak D, et al. An ARC-Regulated IL1β/Cox-2/PGE2/β-Catenin/ARC Circuit Controls Leukemia-Microenvironment Interactions and Confers Drug Resistance in AML. Cancer Res. 2019;79:1165-1177 pubmed publisher
Tcymbarevich I, Eloranta J, Rossel J, Obialo N, Spalinger M, Cosin Roger J, et al. The impact of the rs8005161 polymorphism on G protein-coupled receptor GPR65 (TDAG8) pH-associated activation in intestinal inflammation. BMC Gastroenterol. 2019;19:2 pubmed publisher
Tyurin Kuzmin P, Dyikanov D, Fadeeva J, Sysoeva V, Kalinina N. Flow cytometry analysis of adrenoceptors expression in human adipose-derived mesenchymal stem/stromal cells. Sci Data. 2018;5:180196 pubmed publisher
Santoni de Sio F, Passerini L, Restelli S, Valente M, Pramov A, Maccari M, et al. Role of human forkhead box P3 in early thymic maturation and peripheral T-cell homeostasis. J Allergy Clin Immunol. 2018;142:1909-1921.e9 pubmed publisher
Narasimhan P, Akabas L, Tariq S, Huda N, Bennuru S, Sabzevari H, et al. Similarities and differences between helminth parasites and cancer cell lines in shaping human monocytes: Insights into parallel mechanisms of immune evasion. PLoS Negl Trop Dis. 2018;12:e0006404 pubmed publisher
Du X, Tang F, Liu M, Su J, Zhang Y, Wu W, et al. A reappraisal of CTLA-4 checkpoint blockade in cancer immunotherapy. Cell Res. 2018;28:416-432 pubmed publisher
Logan P, Yango P, Tran N. Endometrial Stromal and Epithelial Cells Exhibit Unique Aberrant Molecular Defects in Patients With Endometriosis. Reprod Sci. 2018;25:140-159 pubmed publisher
Li J, Phadnis Moghe A, Crawford R, Kaminski N. Aryl hydrocarbon receptor activation by 2,3,7,8-tetrachlorodibenzo-p-dioxin impairs human B lymphopoiesis. Toxicology. 2017;378:17-24 pubmed publisher
Crespo Ã, Strominger J, Tilburgs T. Expression of KIR2DS1 by decidual natural killer cells increases their ability to control placental HCMV infection. Proc Natl Acad Sci U S A. 2016;113:15072-15077 pubmed publisher
Skowron K, Pitroda S, Namm J, Balogun O, Beckett M, Zenner M, et al. Basal Tumor Cell Isolation and Patient-Derived Xenograft Engraftment Identify High-Risk Clinical Bladder Cancers. Sci Rep. 2016;6:35854 pubmed publisher
Iannetta M, Zingaropoli M, Bellizzi A, Morreale M, Pontecorvo S, D Abramo A, et al. Natalizumab Affects T-Cell Phenotype in Multiple Sclerosis: Implications for JCV Reactivation. PLoS ONE. 2016;11:e0160277 pubmed publisher
Pham K, Delitto D, Knowlton A, Hartlage E, Madhavan R, Gonzalo D, et al. Isolation of Pancreatic Cancer Cells from a Patient-Derived Xenograft Model Allows for Practical Expansion and Preserved Heterogeneity in Culture. Am J Pathol. 2016;186:1537-46 pubmed publisher
Griffiths K, Dolezal O, Cao B, Nilsson S, See H, Pfleger K, et al. i-bodies, Human Single Domain Antibodies That Antagonize Chemokine Receptor CXCR4. J Biol Chem. 2016;291:12641-57 pubmed publisher
Krampitz G, George B, Willingham S, Volkmer J, Weiskopf K, Jahchan N, et al. Identification of tumorigenic cells and therapeutic targets in pancreatic neuroendocrine tumors. Proc Natl Acad Sci U S A. 2016;113:4464-9 pubmed publisher
Lee J, Breton G, Oliveira T, Zhou Y, Aljoufi A, PUHR S, et al. Restricted dendritic cell and monocyte progenitors in human cord blood and bone marrow. J Exp Med. 2015;212:385-99 pubmed publisher
Breton G, Lee J, Zhou Y, Schreiber J, Keler T, PUHR S, et al. Circulating precursors of human CD1c+ and CD141+ dendritic cells. J Exp Med. 2015;212:401-13 pubmed publisher
Marquardt N, Béziat V, Nyström S, Hengst J, Ivarsson M, Kekäläinen E, et al. Cutting edge: identification and characterization of human intrahepatic CD49a+ NK cells. J Immunol. 2015;194:2467-71 pubmed publisher
Gleason M, Ross J, Warlick E, Lund T, Verneris M, Wiernik A, et al. CD16xCD33 bispecific killer cell engager (BiKE) activates NK cells against primary MDS and MDSC CD33+ targets. Blood. 2014;123:3016-26 pubmed publisher
Bushway M, Gerber S, Fenton B, Miller R, Lord E, Murphy S. Morphological and phenotypic analyses of the human placenta using whole mount immunofluorescence. Biol Reprod. 2014;90:110 pubmed publisher
Hofner T, Macher Goeppinger S, Klein C, Schillert A, Eisen C, Wagner S, et al. Expression and prognostic significance of cancer stem cell markers CD24 and CD44 in urothelial bladder cancer xenografts and patients undergoing radical cystectomy. Urol Oncol. 2014;32:678-86 pubmed publisher
Stoeckle C, Rota I, Tolosa E, Haller C, Melms A, Adamopoulou E. Isolation of myeloid dendritic cells and epithelial cells from human thymus. J Vis Exp. 2013;:e50951 pubmed publisher
Chou S, Byrska Bishop M, Tober J, Yao Y, Vandorn D, Opalinska J, et al. Trisomy 21-associated defects in human primitive hematopoiesis revealed through induced pluripotent stem cells. Proc Natl Acad Sci U S A. 2012;109:17573-8 pubmed publisher
Oeztuerk Winder F, Guinot A, Ochalek A, Ventura J. Regulation of human lung alveolar multipotent cells by a novel p38α MAPK/miR-17-92 axis. EMBO J. 2012;31:3431-41 pubmed publisher
Morozov A, Downey R, Healey J, Moreira A, Lou E, Franceschino A, et al. Benign mesenchymal stromal cells in human sarcomas. Clin Cancer Res. 2010;16:5630-40 pubmed publisher
Jiang Q, Zhang L, Wang R, Jeffrey J, Washburn M, Brouwer D, et al. FoxP3+CD4+ regulatory T cells play an important role in acute HIV-1 infection in humanized Rag2-/-gammaC-/- mice in vivo. Blood. 2008;112:2858-68 pubmed publisher
product information
Antigen :
CD45
Apps. Abbrev. :
Flow Cytometry
Cat # :
304022
Clone :
HI30
Item :
Pacific Blue™ anti-human CD45
Isotype :
Mouse IgG1, κ
Other Names :
LCA, T200
Size :
100 μg
Price (USD) :
225 USD
Reactivity :
Human, Cross-Reactivity: Chimpanzee
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
PB
Application Notes :
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections 9 , inhibition of CD45 functions 4 , immunofluorescence 11 , and Western blotting 3 . It was found that the HI30 clone and the 2D1 clone can cross block each other's binding.
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA