product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Pacific Blue™ anti-human CD45
catalog :
304021
quantity :
25 μg
price :
116 USD
clonality :
monoclonal
host :
mouse
conjugate :
Pacific Blue
clone name :
HI30
reactivity :
human
application :
flow cytometry
more info or order :
citations: 65
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human
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Zee B, Poels K, Yao C, Kawabata K, Wu G, Duy C, et al. Combined epigenetic and metabolic treatments overcome differentiation blockade in acute myeloid leukemia. iScience. 2021;24:102651 pubmed publisher
Ferrari S, Beretta S, Jacob A, Cittaro D, Albano L, Merelli I, et al. BAR-Seq clonal tracking of gene-edited cells. Nat Protoc. 2021;16:2991-3025 pubmed publisher
Caduff N, McHugh D, Rieble L, Forconi C, Ong echa J, Oluoch P, et al. KSHV infection drives poorly cytotoxic CD56-negative natural killer cell differentiation in vivo upon KSHV/EBV dual infection. Cell Rep. 2021;35:109056 pubmed publisher
Iwabuchi R, Ide K, Terahara K, Wagatsuma R, Iwaki R, Matsunaga H, et al. Development of an Inflammatory CD14+ Dendritic Cell Subset in Humanized Mice. Front Immunol. 2021;12:643040 pubmed publisher
Mathewson N, Ashenberg O, Tirosh I, Gritsch S, Perez E, Marx S, et al. Inhibitory CD161 receptor identified in glioma-infiltrating T cells by single-cell analysis. Cell. 2021;184:1281-1298.e26 pubmed publisher
Bonifacius A, Tischer Zimmermann S, Dragon A, Gussarow D, Vogel A, Krettek U, et al. COVID-19 immune signatures reveal stable antiviral T cell function despite declining humoral responses. Immunity. 2021;54:340-354.e6 pubmed publisher
Kenswil K, Pisterzi P, Sanchez Duffhues G, van Dijk C, Lolli A, Knuth C, et al. Endothelium-derived stromal cells contribute to hematopoietic bone marrow niche formation. Cell Stem Cell. 2021;28:653-670.e11 pubmed publisher
Riether C, Radpour R, Kallen N, Bürgin D, Bachmann C, Schürch C, et al. Metoclopramide treatment blocks CD93-signaling-mediated self-renewal of chronic myeloid leukemia stem cells. Cell Rep. 2021;34:108663 pubmed publisher
Chagraoui J, Girard S, Spinella J, Simon L, Bonneil E, Mayotte N, et al. UM171 Preserves Epigenetic Marks that Are Reduced in Ex Vivo Culture of Human HSCs via Potentiation of the CLR3-KBTBD4 Complex. Cell Stem Cell. 2021;28:48-62.e6 pubmed publisher
Marsden M, Zhang T, Du Y, Dimapasoc M, Soliman M, Wu X, et al. Tracking HIV Rebound following Latency Reversal Using Barcoded HIV. Cell Rep Med. 2020;1:100162 pubmed publisher
Lu Y, Liao J, Su S. Protocol for Single-Cell Analysis of Tumor-Infiltrating B Cells Isolated from Human Breast Cancer Tissue Before and After Neo-adjuvant Chemotherapy. STAR Protoc. 2020;1:100040 pubmed publisher
Goodnough L, Ambrosi T, Steininger H, DeBaun M, Abrams G, McAdams T, et al. Delayed Union of a Diaphyseal Forearm Fracture Associated With Impaired Osteogenic Differentiation of Prospectively Isolated Human Skeletal Stem Cells. JBMR Plus. 2020;4:e10398 pubmed publisher
Shin J, Schröder M, Caiado F, Wyman S, Bray N, Bordi M, et al. Controlled Cycling and Quiescence Enables Efficient HDR in Engraftment-Enriched Adult Hematopoietic Stem and Progenitor Cells. Cell Rep. 2020;32:108093 pubmed publisher
Jandke A, Melandri D, Monin L, Ushakov D, Laing A, Vantourout P, et al. Butyrophilin-like proteins display combinatorial diversity in selecting and maintaining signature intraepithelial γδ T cell compartments. Nat Commun. 2020;11:3769 pubmed publisher
Mender I, Zhang A, Ren Z, Han C, Deng Y, Siteni S, et al. Telomere Stress Potentiates STING-Dependent Anti-tumor Immunity. Cancer Cell. 2020;38:400-411.e6 pubmed publisher
Luoma A, Suo S, Williams H, Sharova T, Sullivan K, Manos M, et al. Molecular Pathways of Colon Inflammation Induced by Cancer Immunotherapy. Cell. 2020;: pubmed publisher
Borrelli M, Patel R, Adem S, Diaz Deleon N, Shen A, Sokol J, et al. The antifibrotic adipose-derived stromal cell: Grafted fat enriched with CD74+ adipose-derived stromal cells reduces chronic radiation-induced skin fibrosis. Stem Cells Transl Med. 2020;9:1401-1413 pubmed publisher
Chen Z, Yang X, Bi G, Liang J, Hu Z, Zhao M, et al. Ligand-receptor interaction atlas within and between tumor cells and T cells in lung adenocarcinoma. Int J Biol Sci. 2020;16:2205-2219 pubmed publisher
Borrelli M, Patel R, Blackshear C, Vistnes S, Diaz Deleon N, Adem S, et al. CD34+CD146+ adipose-derived stromal cells enhance engraftment of transplanted fat. Stem Cells Transl Med. 2020;9:1389-1400 pubmed publisher
Zah E, Nam E, Bhuvan V, Tran U, Ji B, Gosliner S, et al. Systematically optimized BCMA/CS1 bispecific CAR-T cells robustly control heterogeneous multiple myeloma. Nat Commun. 2020;11:2283 pubmed publisher
Lu Y, Zhao Q, Liao J, Song E, Xia Q, Pan J, et al. Complement Signals Determine Opposite Effects of B Cells in Chemotherapy-Induced Immunity. Cell. 2020;180:1081-1097.e24 pubmed publisher
Li S, Goncalves K, Lyu B, Yuan L, Hu G. Chemosensitization of prostate cancer stem cells in mice by angiogenin and plexin-B2 inhibitors. Commun Biol. 2020;3:26 pubmed publisher
Queckborner S, Syk Lundberg E, Gemzell Danielsson K, Davies L. Endometrial stromal cells exhibit a distinct phenotypic and immunomodulatory profile. Stem Cell Res Ther. 2020;11:15 pubmed publisher
Rizzo M, Crawford R, Bach A, Sermet S, Amalfitano A, Kaminski N. Δ9-Tetrahydrocannabinol Suppresses Monocyte-Mediated Astrocyte Production of Monocyte Chemoattractant Protein 1 and Interleukin-6 in a Toll-Like Receptor 7-Stimulated Human Coculture. J Pharmacol Exp Ther. 2019;371:191-201 pubmed publisher
Tomellini E, Fares I, Lehnertz B, Chagraoui J, Mayotte N, Macrae T, et al. Integrin-α3 Is a Functional Marker of Ex Vivo Expanded Human Long-Term Hematopoietic Stem Cells. Cell Rep. 2019;28:1063-1073.e5 pubmed publisher
Baccelli I, Gareau Y, Lehnertz B, Gingras S, Spinella J, Corneau S, et al. Mubritinib Targets the Electron Transport Chain Complex I and Reveals the Landscape of OXPHOS Dependency in Acute Myeloid Leukemia. Cancer Cell. 2019;36:84-99.e8 pubmed publisher
Zhang Y, Du X, Liu M, Tang F, Zhang P, Ai C, et al. Hijacking antibody-induced CTLA-4 lysosomal degradation for safer and more effective cancer immunotherapy. Cell Res. 2019;29:609-627 pubmed publisher
Okumura T, Horie Y, Lai C, Lin H, Shoda H, Natsumoto B, et al. Robust and highly efficient hiPSC generation from patient non-mobilized peripheral blood-derived CD34+ cells using the auto-erasable Sendai virus vector. Stem Cell Res Ther. 2019;10:185 pubmed publisher
Beringer A, Molle J, Bartosch B, Miossec P. Two phase kinetics of the inflammatory response from hepatocyte-peripheral blood mononuclear cell interactions. Sci Rep. 2019;9:8378 pubmed publisher
Rizzo M, Crawford R, Bach A, Sermet S, Amalfitano A, Kaminski N. Imiquimod and interferon-alpha augment monocyte-mediated astrocyte secretion of MCP-1, IL-6 and IP-10 in a human co-culture system. J Neuroimmunol. 2019;333:576969 pubmed publisher
Zimmerman K, Bentley M, Lever J, Li Z, Crossman D, Song C, et al. Single-Cell RNA Sequencing Identifies Candidate Renal Resident Macrophage Gene Expression Signatures across Species. J Am Soc Nephrol. 2019;30:767-781 pubmed publisher
Schiroli G, Conti A, Ferrari S, Della Volpe L, Jacob A, Albano L, et al. Precise Gene Editing Preserves Hematopoietic Stem Cell Function following Transient p53-Mediated DNA Damage Response. Cell Stem Cell. 2019;24:551-565.e8 pubmed publisher
Carter B, Mak P, Wang X, Tao W, Ruvolo V, Mak D, et al. An ARC-Regulated IL1β/Cox-2/PGE2/β-Catenin/ARC Circuit Controls Leukemia-Microenvironment Interactions and Confers Drug Resistance in AML. Cancer Res. 2019;79:1165-1177 pubmed publisher
Tcymbarevich I, Eloranta J, Rossel J, Obialo N, Spalinger M, Cosin Roger J, et al. The impact of the rs8005161 polymorphism on G protein-coupled receptor GPR65 (TDAG8) pH-associated activation in intestinal inflammation. BMC Gastroenterol. 2019;19:2 pubmed publisher
Legrand F, Cao Y, Wechsler J, Zhu X, Zimmermann N, Rampertaap S, et al. Sialic acid-binding immunoglobulin-like lectin (Siglec) 8 in patients with eosinophilic disorders: Receptor expression and targeting using chimeric antibodies. J Allergy Clin Immunol. 2019;143:2227-2237.e10 pubmed publisher
Tyurin Kuzmin P, Dyikanov D, Fadeeva J, Sysoeva V, Kalinina N. Flow cytometry analysis of adrenoceptors expression in human adipose-derived mesenchymal stem/stromal cells. Sci Data. 2018;5:180196 pubmed publisher
Santoni de Sio F, Passerini L, Restelli S, Valente M, Pramov A, Maccari M, et al. Role of human forkhead box P3 in early thymic maturation and peripheral T-cell homeostasis. J Allergy Clin Immunol. 2018;142:1909-1921.e9 pubmed publisher
Narasimhan P, Akabas L, Tariq S, Huda N, Bennuru S, Sabzevari H, et al. Similarities and differences between helminth parasites and cancer cell lines in shaping human monocytes: Insights into parallel mechanisms of immune evasion. PLoS Negl Trop Dis. 2018;12:e0006404 pubmed publisher
Du X, Tang F, Liu M, Su J, Zhang Y, Wu W, et al. A reappraisal of CTLA-4 checkpoint blockade in cancer immunotherapy. Cell Res. 2018;28:416-432 pubmed publisher
Logan P, Yango P, Tran N. Endometrial Stromal and Epithelial Cells Exhibit Unique Aberrant Molecular Defects in Patients With Endometriosis. Reprod Sci. 2018;25:140-159 pubmed publisher
Li J, Phadnis Moghe A, Crawford R, Kaminski N. Aryl hydrocarbon receptor activation by 2,3,7,8-tetrachlorodibenzo-p-dioxin impairs human B lymphopoiesis. Toxicology. 2017;378:17-24 pubmed publisher
Crespo Ã, Strominger J, Tilburgs T. Expression of KIR2DS1 by decidual natural killer cells increases their ability to control placental HCMV infection. Proc Natl Acad Sci U S A. 2016;113:15072-15077 pubmed publisher
Skowron K, Pitroda S, Namm J, Balogun O, Beckett M, Zenner M, et al. Basal Tumor Cell Isolation and Patient-Derived Xenograft Engraftment Identify High-Risk Clinical Bladder Cancers. Sci Rep. 2016;6:35854 pubmed publisher
Iannetta M, Zingaropoli M, Bellizzi A, Morreale M, Pontecorvo S, D Abramo A, et al. Natalizumab Affects T-Cell Phenotype in Multiple Sclerosis: Implications for JCV Reactivation. PLoS ONE. 2016;11:e0160277 pubmed publisher
Pham K, Delitto D, Knowlton A, Hartlage E, Madhavan R, Gonzalo D, et al. Isolation of Pancreatic Cancer Cells from a Patient-Derived Xenograft Model Allows for Practical Expansion and Preserved Heterogeneity in Culture. Am J Pathol. 2016;186:1537-46 pubmed publisher
Griffiths K, Dolezal O, Cao B, Nilsson S, See H, Pfleger K, et al. i-bodies, Human Single Domain Antibodies That Antagonize Chemokine Receptor CXCR4. J Biol Chem. 2016;291:12641-57 pubmed publisher
Krampitz G, George B, Willingham S, Volkmer J, Weiskopf K, Jahchan N, et al. Identification of tumorigenic cells and therapeutic targets in pancreatic neuroendocrine tumors. Proc Natl Acad Sci U S A. 2016;113:4464-9 pubmed publisher
Cheah M, Chen J, Sahoo D, Contreras Trujillo H, Volkmer A, Scheeren F, et al. CD14-expressing cancer cells establish the inflammatory and proliferative tumor microenvironment in bladder cancer. Proc Natl Acad Sci U S A. 2015;112:4725-30 pubmed publisher
Lee J, Breton G, Oliveira T, Zhou Y, Aljoufi A, PUHR S, et al. Restricted dendritic cell and monocyte progenitors in human cord blood and bone marrow. J Exp Med. 2015;212:385-99 pubmed publisher
Breton G, Lee J, Zhou Y, Schreiber J, Keler T, PUHR S, et al. Circulating precursors of human CD1c+ and CD141+ dendritic cells. J Exp Med. 2015;212:401-13 pubmed publisher
Marquardt N, Béziat V, Nyström S, Hengst J, Ivarsson M, Kekäläinen E, et al. Cutting edge: identification and characterization of human intrahepatic CD49a+ NK cells. J Immunol. 2015;194:2467-71 pubmed publisher
Valle A, Barbagiovanni G, Jofra T, Stabilini A, Pérol L, Baeyens A, et al. Heterogeneous CD3 expression levels in differing T cell subsets correlate with the in vivo anti-CD3-mediated T cell modulation. J Immunol. 2015;194:2117-27 pubmed publisher
Gleason M, Ross J, Warlick E, Lund T, Verneris M, Wiernik A, et al. CD16xCD33 bispecific killer cell engager (BiKE) activates NK cells against primary MDS and MDSC CD33+ targets. Blood. 2014;123:3016-26 pubmed publisher
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Chou S, Byrska Bishop M, Tober J, Yao Y, Vandorn D, Opalinska J, et al. Trisomy 21-associated defects in human primitive hematopoiesis revealed through induced pluripotent stem cells. Proc Natl Acad Sci U S A. 2012;109:17573-8 pubmed publisher
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Jiang Q, Zhang L, Wang R, Jeffrey J, Washburn M, Brouwer D, et al. FoxP3+CD4+ regulatory T cells play an important role in acute HIV-1 infection in humanized Rag2-/-gammaC-/- mice in vivo. Blood. 2008;112:2858-68 pubmed publisher
product information
Antigen :
CD45
Apps. Abbrev. :
Flow Cytometry
Cat # :
304021
Clone :
HI30
Item :
Pacific Blue™ anti-human CD45
Isotype :
Mouse IgG1, κ
Other Names :
LCA, T200
Size :
25 μg
Price (USD) :
116 USD
Reactivity :
Human, Cross-Reactivity: Chimpanzee
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
PB
Application Notes :
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections 9 , inhibition of CD45 functions 4 , immunofluorescence 11 , Western blotting 3 , and spatial biology (IBEX) 16,17 . It was found that the HI30 clone and the 2D1 clone can cross block each other s binding.
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA