product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Alexa Fluor® 488 anti-human CD45
catalog :
304017
quantity :
100 tests
price :
242 USD
clonality :
monoclonal
host :
mouse
conjugate :
AF488
clone name :
HI30
reactivity :
human, mouse
application :
immunocytochemistry, flow cytometry, immunohistochemistry - frozen section, mass cytometry
more info or order :
citations: 60
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; loading ...; fig 1f, s1b
Wu X, Xia T, Shin W, Yu K, Jung W, Herrmann A, et al. Viral Mimicry of Interleukin-17A by SARS-CoV-2 ORF8. MBio. 2022;13:e0040222 pubmed publisher
  • mass cytometry; human; loading ...; fig 4e
Ingelfinger F, Krishnarajah S, Kramer M, Utz S, Galli E, Lutz M, et al. Single-cell profiling of myasthenia gravis identifies a pathogenic T cell signature. Acta Neuropathol. 2021;141:901-915 pubmed publisher
  • flow cytometry; human; loading ...
Vavassori V, Mercuri E, Marcovecchio G, Castiello M, Schiroli G, Albano L, et al. Modeling, optimization, and comparable efficacy of T cell and hematopoietic stem cell gene editing for treating hyper-IgM syndrome. EMBO Mol Med. 2021;13:e13545 pubmed publisher
  • flow cytometry; human; loading ...; fig 5a
Harro C, Perez Sanz J, Costich T, Payne K, Anadon C, Chaurio R, et al. Methyltransferase inhibitors restore SATB1 protective activity against cutaneous T cell lymphoma in mice. J Clin Invest. 2021;131: pubmed publisher
  • flow cytometry; human; loading ...; fig s1
Katano I, Ito R, Kawai K, Takahashi T. Improved Detection of in vivo Human NK Cell-Mediated Antibody-Dependent Cellular Cytotoxicity Using a Novel NOG-FcγR-Deficient Human IL-15 Transgenic Mouse. Front Immunol. 2020;11:532684 pubmed publisher
  • immunocytochemistry; human; 10 ug/ml; loading ...; fig 2b
Ortiz Otero N, Marshall J, Lash B, King M. Chemotherapy-induced release of circulating-tumor cells into the bloodstream in collective migration units with cancer-associated fibroblasts in metastatic cancer patients. BMC Cancer. 2020;20:873 pubmed publisher
  • flow cytometry; human; loading ...; fig 1b
Stary V, Wolf B, Unterleuthner D, List J, Talic M, Laengle J, et al. Short-course radiotherapy promotes pro-inflammatory macrophages via extracellular vesicles in human rectal cancer. J Immunother Cancer. 2020;8: pubmed publisher
  • immunohistochemistry - frozen section; mouse; loading ...; fig 9a
Kaaij M, van Tok M, Blijdorp I, Ambarus C, Stock M, Pots D, et al. Transmembrane TNF drives osteoproliferative joint inflammation reminiscent of human spondyloarthritis. J Exp Med. 2020;217: pubmed publisher
  • flow cytometry; human; loading ...
Wong S, Lenzini S, Cooper M, Mooney D, Shin J. Soft extracellular matrix enhances inflammatory activation of mesenchymal stromal cells to induce monocyte production and trafficking. Sci Adv. 2020;6:eaaw0158 pubmed publisher
  • mass cytometry; human; loading ...; fig 1b, 1d, s2
Leylek R, Alcántara Hernández M, Lanzar Z, Lüdtke A, Perez O, Reizis B, et al. Integrated Cross-Species Analysis Identifies a Conserved Transitional Dendritic Cell Population. Cell Rep. 2019;29:3736-3750.e8 pubmed publisher
  • immunocytochemistry; human; 1:250; fig 2a
Bokun V, Moore J, Moore R, Smallcombe C, Harford T, Rezaee F, et al. Respiratory syncytial virus exhibits differential tropism for distinct human placental cell types with Hofbauer cells acting as a permissive reservoir for infection. PLoS ONE. 2019;14:e0225767 pubmed publisher
  • flow cytometry; human; loading ...; fig s14b
  • flow cytometry; mouse; loading ...; fig s19b
Stewart B, Ferdinand J, Young M, Mitchell T, Loudon K, Riding A, et al. Spatiotemporal immune zonation of the human kidney. Science. 2019;365:1461-1466 pubmed publisher
  • flow cytometry; human; loading ...; fig 1a
Allden S, Ogger P, Ghai P, McErlean P, Hewitt R, Toshner R, et al. The Transferrin Receptor CD71 Delineates Functionally Distinct Airway Macrophage Subsets during Idiopathic Pulmonary Fibrosis. Am J Respir Crit Care Med. 2019;: pubmed publisher
  • mass cytometry; human; loading ...; fig s1
Fernandez I, Baxter R, Garcia Perez J, Vendrame E, Ranganath T, Kong D, et al. A novel human IL2RB mutation results in T and NK cell-driven immune dysregulation. J Exp Med. 2019;216:1255-1267 pubmed publisher
  • flow cytometry; human; loading ...; fig s3
Lim S, Kim J, Jeon S, Shin M, Kwon J, Kim T, et al. Defective Localization With Impaired Tumor Cytotoxicity Contributes to the Immune Escape of NK Cells in Pancreatic Cancer Patients. Front Immunol. 2019;10:496 pubmed publisher
  • flow cytometry; human; fig 1c
Montel Hagen A, Seet C, Li S, Chick B, Zhu Y, Chang P, et al. Organoid-Induced Differentiation of Conventional T Cells from Human Pluripotent Stem Cells. Cell Stem Cell. 2019;24:376-389.e8 pubmed publisher
  • flow cytometry; human; loading ...; tbl s1
Dias J, Boulouis C, Gorin J, van den Biggelaar R, Lal K, Gibbs A, et al. The CD4-CD8- MAIT cell subpopulation is a functionally distinct subset developmentally related to the main CD8+ MAIT cell pool. Proc Natl Acad Sci U S A. 2018;115:E11513-E11522 pubmed publisher
  • flow cytometry; human; loading ...; fig s3b
Arnold I, Artola Borán M, Tallón de Lara P, Kyburz A, Taube C, OTTEMANN K, et al. Eosinophils suppress Th1 responses and restrict bacterially induced gastrointestinal inflammation. J Exp Med. 2018;215:2055-2072 pubmed publisher
  • flow cytometry; human; loading ...; fig 9b
Casey A, Sinha A, Singhania R, Livingstone J, Waterhouse P, Tharmapalan P, et al. Mammary molecular portraits reveal lineage-specific features and progenitor cell vulnerabilities. J Cell Biol. 2018;217:2951-2974 pubmed publisher
  • flow cytometry; human; fig 1a
Bujko A, Atlasy N, Landsverk O, Richter L, Yaqub S, Horneland R, et al. Transcriptional and functional profiling defines human small intestinal macrophage subsets. J Exp Med. 2018;215:441-458 pubmed publisher
  • flow cytometry; human; loading ...; fig 1c
Herndler Brandstetter D, Shan L, Yao Y, Stecher C, Plajer V, Lietzenmayer M, et al. Humanized mouse model supports development, function, and tissue residency of human natural killer cells. Proc Natl Acad Sci U S A. 2017;114:E9626-E9634 pubmed publisher
  • flow cytometry; human; loading ...; fig 6c
Daley D, Mani V, Mohan N, Akkad N, Ochi A, Heindel D, et al. Dectin 1 activation on macrophages by galectin 9 promotes pancreatic carcinoma and peritumoral immune tolerance. Nat Med. 2017;23:556-567 pubmed publisher
  • flow cytometry; human; loading ...; fig 1a
Wei C, Mei J, Tang L, Liu Y, Li D, Li M, et al. 1-Methyl-tryptophan attenuates regulatory T cells differentiation due to the inhibition of estrogen-IDO1-MRC2 axis in endometriosis. Cell Death Dis. 2016;7:e2489 pubmed publisher
  • flow cytometry; human; loading ...; fig 3a
Faivre V, Lukaszewicz A, Payen D. Downregulation of Blood Monocyte HLA-DR in ICU Patients Is Also Present in Bone Marrow Cells. PLoS ONE. 2016;11:e0164489 pubmed publisher
  • flow cytometry; human; loading ...; fig s1
Landtwing V, Raykova A, Pezzino G, Beziat V, Marcenaro E, Graf C, et al. Cognate HLA absence in trans diminishes human NK cell education. J Clin Invest. 2016;126:3772-3782 pubmed publisher
  • flow cytometry; human; loading ...; fig 1a
Bal S, Bernink J, Nagasawa M, Groot J, Shikhagaie M, Golebski K, et al. IL-1?, IL-4 and IL-12 control the fate of group 2 innate lymphoid cells in human airway inflammation in the lungs. Nat Immunol. 2016;17:636-45 pubmed publisher
  • flow cytometry; human
Bendall S, Simonds E, Qiu P, Amir E, Krutzik P, Finck R, et al. Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum. Science. 2011;332:687-96 pubmed publisher
Guahmich N, Man L, Wang J, Arazi L, Kallinos E, Topper Kroog A, et al. Human theca arises from ovarian stroma and is comprised of three discrete subtypes. Commun Biol. 2023;6:7 pubmed publisher
Liu T, Han C, Fang P, Ma Z, Wang X, Chen H, et al. Cancer-associated fibroblast-specific lncRNA LINC01614 enhances glutamine uptake in lung adenocarcinoma. J Hematol Oncol. 2022;15:141 pubmed publisher
Zachara M, Rainer P, Hashimi H, Russeil J, Alpern D, Ferrero R, et al. Mammalian adipogenesis regulator (Areg) cells use retinoic acid signalling to be non- and anti-adipogenic in age-dependent manner. EMBO J. 2022;41:e108206 pubmed publisher
Wang H, Chen L, Qi L, Jiang N, Zhang Z, Guo H, et al. A Single-Cell Atlas of Tumor-Infiltrating Immune Cells in Pancreatic Ductal Adenocarcinoma. Mol Cell Proteomics. 2022;21:100258 pubmed publisher
Brian B, Sjaastad F, Freedman T. SH3-domain mutations selectively disrupt Csk homodimerization or PTPN22 binding. Sci Rep. 2022;12:5875 pubmed publisher
Farshidfar F, Rhrissorrakrai K, Levovitz C, Peng C, Knight J, Bacchiocchi A, et al. Integrative molecular and clinical profiling of acral melanoma links focal amplification of 22q11.21 to metastasis. Nat Commun. 2022;13:898 pubmed publisher
Johnson K, Anderson K, Courtois E, Gujar A, Barthel F, Varn F, et al. Single-cell multimodal glioma analyses identify epigenetic regulators of cellular plasticity and environmental stress response. Nat Genet. 2021;53:1456-1468 pubmed publisher
Hendricks A, Dall K, Brandt B, Geisen R, R xf6 der C, Schafmayer C, et al. Longitudinal Analysis of Circulating Tumor Cells in Colorectal Cancer Patients by a Cytological and Molecular Approach: Feasibility and Clinical Application. Front Oncol. 2021;11:646885 pubmed publisher
Jung Y, Wen L, Altman A, Ley K. CD45 pre-exclusion from the tips of T cell microvilli prior to antigen recognition. Nat Commun. 2021;12:3872 pubmed publisher
Ji S, Gumina D, McPeak K, Moldovan R, Post M, Su E. Human placental villous stromal extracellular matrix regulates fetoplacental angiogenesis in severe fetal growth restriction. Clin Sci (Lond). 2021;135:1127-1143 pubmed publisher
Dam T, Junghans V, Humphrey J, Chouliara M, J xf6 nsson P. Calcium Signaling in T Cells Is Induced by Binding to Nickel-Chelating Lipids in Supported Lipid Bilayers. Front Physiol. 2020;11:613367 pubmed publisher
Li T, Li H, Zhang W, Xu S, Jiang W, Li S, et al. Human splenic TER cells: A relevant prognostic factor acting via the artemin-GFRα3-ERK pathway in pancreatic ductal adenocarcinoma. Int J Cancer. 2021;148:1756-1767 pubmed publisher
Takeda A, Jalkanen S. Single-Cell Transcriptomics of Human Lymph Node Stroma. STAR Protoc. 2020;1:100021 pubmed publisher
O Donovan B, Mandel Brehm C, Vazquez S, Liu J, Parent A, Anderson M, et al. High-resolution epitope mapping of anti-Hu and anti-Yo autoimmunity by programmable phage display. Brain Commun. 2020;2:fcaa059 pubmed publisher
Hendricks A, Brandt B, Geisen R, Dall K, Roder C, Schafmayer C, et al. Isolation and Enumeration of CTC in Colorectal Cancer Patients: Introduction of a Novel Cell Imaging Approach and Comparison to Cellular and Molecular Detection Techniques. Cancers (Basel). 2020;12: pubmed publisher
Man L, Lustgarten Guahmich N, Kallinos E, Redhead Laconte Z, Liu S, Schattman B, et al. Comparison of Human Antral Follicles of Xenograft versus Ovarian Origin Reveals Disparate Molecular Signatures. Cell Rep. 2020;32:108027 pubmed publisher
Mellergaard M, Høgh R, Lund A, Aldana B, Guérillot R, Møller S, et al. Staphylococcus aureus induces cell-surface expression of immune stimulatory NKG2D ligands on human monocytes. J Biol Chem. 2020;295:11803-11821 pubmed publisher
Klemm F, Maas R, Bowman R, Kornete M, Soukup K, Nassiri S, et al. Interrogation of the Microenvironmental Landscape in Brain Tumors Reveals Disease-Specific Alterations of Immune Cells. Cell. 2020;181:1643-1660.e17 pubmed publisher
Özcan A, Sahin D, Impellizzieri D, Nguyen T, Hafner J, Yawalkar N, et al. Nanoparticle-Coupled Topical Methotrexate Can Normalize Immune Responses and Induce Tissue Remodeling in Psoriasis. J Invest Dermatol. 2020;140:1003-1014.e8 pubmed publisher
Sakai M, Troutman T, Seidman J, Ouyang Z, Spann N, Abe Y, et al. Liver-Derived Signals Sequentially Reprogram Myeloid Enhancers to Initiate and Maintain Kupffer Cell Identity. Immunity. 2019;51:655-670.e8 pubmed publisher
Meehan S, Kolyagin G, Parks D, Youngyunpipatkul J, Herzenberg L, Walther G, et al. Automated subset identification and characterization pipeline for multidimensional flow and mass cytometry data clustering and visualization. Commun Biol. 2019;2:229 pubmed publisher
VanderBurgh J, Hotchkiss H, Potharazu A, Taufalele P, Reinhart King C. Substrate stiffness heterogeneities disrupt endothelial barrier integrity in a micropillar model of heterogeneous vascular stiffening. Integr Biol (Camb). 2018;10:734-746 pubmed publisher
Earl D, Ferrell P, Leelatian N, Froese J, Reisman B, Irish J, et al. Discovery of human cell selective effector molecules using single cell multiplexed activity metabolomics. Nat Commun. 2018;9:39 pubmed publisher
Passaro D, di Tullio A, Abarrategi A, Rouault Pierre K, Foster K, Ariza McNaughton L, et al. Increased Vascular Permeability in the Bone Marrow Microenvironment Contributes to Disease Progression and Drug Response in Acute Myeloid Leukemia. Cancer Cell. 2017;32:324-341.e6 pubmed publisher
Gonzales L, Gonzalez R, Barrette A, Wang P, Dobbs L, Ballard P. Expression of Carcinoembryonic Cell Adhesion Molecule 6 and Alveolar Epithelial Cell Markers in Lungs of Human Infants with Chronic Lung Disease. J Histochem Cytochem. 2015;63:908-21 pubmed publisher
Lee J, Breton G, Oliveira T, Zhou Y, Aljoufi A, PUHR S, et al. Restricted dendritic cell and monocyte progenitors in human cord blood and bone marrow. J Exp Med. 2015;212:385-99 pubmed publisher
Breton G, Lee J, Zhou Y, Schreiber J, Keler T, PUHR S, et al. Circulating precursors of human CD1c+ and CD141+ dendritic cells. J Exp Med. 2015;212:401-13 pubmed publisher
Marquardt N, Béziat V, Nyström S, Hengst J, Ivarsson M, Kekäläinen E, et al. Cutting edge: identification and characterization of human intrahepatic CD49a+ NK cells. J Immunol. 2015;194:2467-71 pubmed publisher
Bushway M, Gerber S, Fenton B, Miller R, Lord E, Murphy S. Morphological and phenotypic analyses of the human placenta using whole mount immunofluorescence. Biol Reprod. 2014;90:110 pubmed publisher
Bashour K, Gondarenko A, Chen H, Shen K, Liu X, Huse M, et al. CD28 and CD3 have complementary roles in T-cell traction forces. Proc Natl Acad Sci U S A. 2014;111:2241-6 pubmed publisher
Oeztuerk Winder F, Guinot A, Ochalek A, Ventura J. Regulation of human lung alveolar multipotent cells by a novel p38α MAPK/miR-17-92 axis. EMBO J. 2012;31:3431-41 pubmed publisher
Morozov A, Downey R, Healey J, Moreira A, Lou E, Franceschino A, et al. Benign mesenchymal stromal cells in human sarcomas. Clin Cancer Res. 2010;16:5630-40 pubmed publisher
Jiang Q, Zhang L, Wang R, Jeffrey J, Washburn M, Brouwer D, et al. FoxP3+CD4+ regulatory T cells play an important role in acute HIV-1 infection in humanized Rag2-/-gammaC-/- mice in vivo. Blood. 2008;112:2858-68 pubmed publisher
product information
Antigen :
CD45
Apps. Abbrev. :
FC
Cat # :
304017
Clone :
HI30
Item :
Alexa Fluor® 488 anti-human CD45
Isotype :
Mouse IgG1, κ
Other Names :
LCA, T200
Size :
100 tests
Price (USD) :
242 USD
Reactivity :
Human
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
A488
Application Notes :
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections^9, inhibition of CD45 functions^4, immunofluorescence^11, Western blotting^3, and spatial biology (IBEX)^16,17.It was found that the HI30 clone and the 2D1 clone can cross block each other's binding.;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA