product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Alexa Fluor® 594 anti-human CD31
catalog :
303126
quantity :
100 μg
price :
288 USD
clonality :
monoclonal
host :
mouse
conjugate :
Alexa Fluor 594
clone name :
WM59
reactivity :
human, rhesus macaque
application :
immunohistochemistry, immunocytochemistry, flow cytometry
more info or order :
citations: 15
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; human; 1:500; fig 4a
Yuge S, Nishiyama K, Arima Y, Hanada Y, Oguri Nakamura E, Hanada S, et al. Mechanical loading of intraluminal pressure mediates wound angiogenesis by regulating the TOCA family of F-BAR proteins. Nat Commun. 2022;13:2594 pubmed publisher
  • flow cytometry; human; 4:100; loading ...; fig 1a
Chioh F, Fong S, Young B, Wu K, Siau A, Krishnan S, et al. Convalescent COVID-19 patients are susceptible to endothelial dysfunction due to persistent immune activation. elife. 2021;10: pubmed publisher
  • immunohistochemistry; human; 1:200; fig 6i
Grüneboom A, Hawwari I, Weidner D, Culemann S, Müller S, Henneberg S, et al. A network of trans-cortical capillaries as mainstay for blood circulation in long bones. Nat Metab. 2019;1:236-250 pubmed publisher
  • immunocytochemistry; human; ; loading ...; fig 3a, 3b
King D, Glynn M, Cindrić S, Kernan D, O Connell T, Hakimjavadi R, et al. Label-Free Multi Parameter Optical Interrogation of Endothelial Activation in Single Cells using a Lab on a Disc Platform. Sci Rep. 2019;9:4157 pubmed publisher
  • flow cytometry; human; fig s1a
Montel Hagen A, Seet C, Li S, Chick B, Zhu Y, Chang P, et al. Organoid-Induced Differentiation of Conventional T Cells from Human Pluripotent Stem Cells. Cell Stem Cell. 2019;24:376-389.e8 pubmed publisher
  • flow cytometry; human; loading ...; fig 9b
Casey A, Sinha A, Singhania R, Livingstone J, Waterhouse P, Tharmapalan P, et al. Mammary molecular portraits reveal lineage-specific features and progenitor cell vulnerabilities. J Cell Biol. 2018;217:2951-2974 pubmed publisher
Kondo H, Ratcliffe C, Hooper S, Ellis J, Macrae J, Hennequart M, et al. Single-cell resolved imaging reveals intra-tumor heterogeneity in glycolysis, transitions between metabolic states, and their regulatory mechanisms. Cell Rep. 2021;34:108750 pubmed publisher
Fenton T, Jørgensen P, Niss K, Rubin S, Mörbe U, Riis L, et al. Immune Profiling of Human Gut-Associated Lymphoid Tissue Identifies a Role for Isolated Lymphoid Follicles in Priming of Region-Specific Immunity. Immunity. 2020;52:557-570.e6 pubmed publisher
Noonan J, Grassia G, Macritchie N, Garside P, Guzik T, Bradshaw A, et al. A Novel Triple-Cell Two-Dimensional Model to Study Immune-Vascular Interplay in Atherosclerosis. Front Immunol. 2019;10:849 pubmed publisher
Noonan J, Asiala S, Grassia G, Macritchie N, Gracie K, Carson J, et al. In vivo multiplex molecular imaging of vascular inflammation using surface-enhanced Raman spectroscopy. Theranostics. 2018;8:6195-6209 pubmed publisher
Bushway M, Gerber S, Fenton B, Miller R, Lord E, Murphy S. Morphological and phenotypic analyses of the human placenta using whole mount immunofluorescence. Biol Reprod. 2014;90:110 pubmed publisher
Oeztuerk Winder F, Guinot A, Ochalek A, Ventura J. Regulation of human lung alveolar multipotent cells by a novel p38α MAPK/miR-17-92 axis. EMBO J. 2012;31:3431-41 pubmed publisher
Wicki A, Rochlitz C, Orleth A, Ritschard R, Albrecht I, Herrmann R, et al. Targeting tumor-associated endothelial cells: anti-VEGFR2 immunoliposomes mediate tumor vessel disruption and inhibit tumor growth. Clin Cancer Res. 2012;18:454-64 pubmed publisher
Nagano M, Yamashita T, Hamada H, Ohneda K, Kimura K, Nakagawa T, et al. Identification of functional endothelial progenitor cells suitable for the treatment of ischemic tissue using human umbilical cord blood. Blood. 2007;110:151-60 pubmed
MacFadyen J, Haworth O, Roberston D, Hardie D, Webster M, Morris H, et al. Endosialin (TEM1, CD248) is a marker of stromal fibroblasts and is not selectively expressed on tumour endothelium. FEBS Lett. 2005;579:2569-75 pubmed
product information
Antigen :
CD31
Apps. Abbrev. :
ICC
Cat # :
303126
Clone :
WM59
Item :
Alexa Fluor® 594 anti-human CD31
Isotype :
Mouse IgG1, κ
Other Names :
PECAM-1, EndoCAM
Size :
100 μg
Price (USD) :
288 USD
Reactivity :
Human, Cynomolgus, Rhesus
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
A594
Application Notes :
Clone WM59 has been reported to recognize the D2 extracellular portion of CD31.Additional reported applications (for the relevant formats) include: immunofluorescence microscopy^2, immunohistochemical staining of acetone-fixed frozen tissue sections^8, blocking of platelet aggregation^3, and spatial biology (IBEX)^11,12. Clone WM59 is not recommended for immunohistochemical staining of formalin-fixed paraffin-embedded sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 303143 & 303144).The purified WM59 antibody is useful as a capture antibody for a sandwich ELISA assay, when used in conjunction with biotin anti-human CD31 antibody (Cat. No. 536604) antibody as the detection antibody.;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA