product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Alexa Fluor® 488 anti-human CD31
catalog :
303109
quantity :
25 tests
price :
124 USD
clonality :
monoclonal
host :
mouse
conjugate :
AF488
clone name :
WM59
reactivity :
human, rhesus macaque
application :
immunocytochemistry, flow cytometry
more info or order :
citations: 28
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; human; 1:500; fig 4a
Yuge S, Nishiyama K, Arima Y, Hanada Y, Oguri Nakamura E, Hanada S, et al. Mechanical loading of intraluminal pressure mediates wound angiogenesis by regulating the TOCA family of F-BAR proteins. Nat Commun. 2022;13:2594 pubmed publisher
  • flow cytometry; human; 4:100; loading ...; fig 1a
Chioh F, Fong S, Young B, Wu K, Siau A, Krishnan S, et al. Convalescent COVID-19 patients are susceptible to endothelial dysfunction due to persistent immune activation. elife. 2021;10: pubmed publisher
  • flow cytometry; human; fig s1a
Montel Hagen A, Seet C, Li S, Chick B, Zhu Y, Chang P, et al. Organoid-Induced Differentiation of Conventional T Cells from Human Pluripotent Stem Cells. Cell Stem Cell. 2019;24:376-389.e8 pubmed publisher
  • flow cytometry; human; loading ...; fig 9b
Casey A, Sinha A, Singhania R, Livingstone J, Waterhouse P, Tharmapalan P, et al. Mammary molecular portraits reveal lineage-specific features and progenitor cell vulnerabilities. J Cell Biol. 2018;217:2951-2974 pubmed publisher
  • immunocytochemistry; human; fig 4
Stanly T, Fritzsche M, Banerji S, Garcia E, Bernardino de la Serna J, Jackson D, et al. Critical importance of appropriate fixation conditions for faithful imaging of receptor microclusters. Biol Open. 2016;5:1343-50 pubmed publisher
  • flow cytometry; human
Anderson E, Mooney D. The Combination of Vascular Endothelial Growth Factor and Stromal Cell-Derived Factor Induces Superior Angiogenic Sprouting by Outgrowth Endothelial Cells. J Vasc Res. 2015;52:62-9 pubmed publisher
Antonyshyn J, Mazzoli V, McFadden M, Gramolini A, Hofer S, Simmons C, et al. Mitigating the non-specific uptake of immunomagnetic microparticles enables the extraction of endothelium from human fat. Commun Biol. 2021;4:1205 pubmed publisher
Tsukada Y, Muramatsu F, Hayashi Y, Inagaki C, Su H, Iba T, et al. An in vivo model allowing continuous observation of human vascular formation in the same animal over time. Sci Rep. 2021;11:745 pubmed publisher
Hedegaard C, Redondo Gómez C, Tan B, Ng K, Loessner D, Mata A. Peptide-protein coassembling matrices as a biomimetic 3D model of ovarian cancer. Sci Adv. 2020;6: pubmed publisher
Teijeira A, Garasa S, Gato M, Alfaro C, Miguéliz I, Cirella A, et al. CXCR1 and CXCR2 Chemokine Receptor Agonists Produced by Tumors Induce Neutrophil Extracellular Traps that Interfere with Immune Cytotoxicity. Immunity. 2020;52:856-871.e8 pubmed publisher
Sarkar T, Quarta M, Mukherjee S, Colville A, Paine P, Doan L, et al. Transient non-integrative expression of nuclear reprogramming factors promotes multifaceted amelioration of aging in human cells. Nat Commun. 2020;11:1545 pubmed publisher
Harvestine J, Gonzalez Fernandez T, Sebastian A, Hum N, Genetos D, Loots G, et al. Osteogenic preconditioning in perfusion bioreactors improves vascularization and bone formation by human bone marrow aspirates. Sci Adv. 2020;6:eaay2387 pubmed publisher
Ajit A, Retnabai S, Krishnan L. Engineered human adipose-derived stem cells inducing endothelial lineage and angiogenic response. Tissue Eng Part C Methods. 2019;: pubmed publisher
Harvestine J, Orbay H, Chen J, Sahar D, Leach J. Cell-secreted extracellular matrix, independent of cell source, promotes the osteogenic differentiation of human stromal vascular fraction. J Mater Chem B. 2018;6:4104-4115 pubmed publisher
Khan O, Kowalski P, Doloff J, Tsosie J, Bakthavatchalu V, Winn C, et al. Endothelial siRNA delivery in nonhuman primates using ionizable low-molecular weight polymeric nanoparticles. Sci Adv. 2018;4:eaar8409 pubmed publisher
Meng Y, Liang J, Wu C, Xu J, Zeng D, Yu X, et al. Monocytes/Macrophages promote vascular CXCR4 expression via the ERK pathway in hepatocellular carcinoma. Oncoimmunology. 2018;7:e1408745 pubmed publisher
Judson R, Quarta M, Oudhoff M, Soliman H, Yi L, Chang C, et al. Inhibition of Methyltransferase Setd7 Allows the In Vitro Expansion of Myogenic Stem Cells with Improved Therapeutic Potential. Cell Stem Cell. 2018;22:177-190.e7 pubmed publisher
Joly P, Schaus T, Sass A, Dienelt A, Cheung A, Duda G, et al. Biophysical induction of cell release for minimally manipulative cell enrichment strategies. PLoS ONE. 2017;12:e0180568 pubmed publisher
Xu J, Liang J, Meng Y, Yan J, Yu X, Liu C, et al. Vascular CXCR4 Expression Promotes Vessel Sprouting and Sensitivity to Sorafenib Treatment in Hepatocellular Carcinoma. Clin Cancer Res. 2017;23:4482-4492 pubmed publisher
Bradfield P, Menon A, Miljkovic Licina M, Lee B, Fischer N, Fish R, et al. Divergent JAM-C Expression Accelerates Monocyte-Derived Cell Exit from Atherosclerotic Plaques. PLoS ONE. 2016;11:e0159679 pubmed publisher
Quarta M, Brett J, DiMarco R, de Morrée A, Boutet S, Chacon R, et al. An artificial niche preserves the quiescence of muscle stem cells and enhances their therapeutic efficacy. Nat Biotechnol. 2016;34:752-9 pubmed publisher
Kumashiro Y, Ishihara J, Umemoto T, Itoga K, Kobayashi J, Shimizu T, et al. Stripe-patterned thermo-responsive cell culture dish for cell separation without cell labeling. Small. 2015;11:681-7 pubmed publisher
Bushway M, Gerber S, Fenton B, Miller R, Lord E, Murphy S. Morphological and phenotypic analyses of the human placenta using whole mount immunofluorescence. Biol Reprod. 2014;90:110 pubmed publisher
Oeztuerk Winder F, Guinot A, Ochalek A, Ventura J. Regulation of human lung alveolar multipotent cells by a novel p38α MAPK/miR-17-92 axis. EMBO J. 2012;31:3431-41 pubmed publisher
Wicki A, Rochlitz C, Orleth A, Ritschard R, Albrecht I, Herrmann R, et al. Targeting tumor-associated endothelial cells: anti-VEGFR2 immunoliposomes mediate tumor vessel disruption and inhibit tumor growth. Clin Cancer Res. 2012;18:454-64 pubmed publisher
Hu Lowe D, Chen E, Zhang L, Watson K, Mancuso P, Lappin P, et al. Targeting activin receptor-like kinase 1 inhibits angiogenesis and tumorigenesis through a mechanism of action complementary to anti-VEGF therapies. Cancer Res. 2011;71:1362-73 pubmed publisher
Nagano M, Yamashita T, Hamada H, Ohneda K, Kimura K, Nakagawa T, et al. Identification of functional endothelial progenitor cells suitable for the treatment of ischemic tissue using human umbilical cord blood. Blood. 2007;110:151-60 pubmed
MacFadyen J, Haworth O, Roberston D, Hardie D, Webster M, Morris H, et al. Endosialin (TEM1, CD248) is a marker of stromal fibroblasts and is not selectively expressed on tumour endothelium. FEBS Lett. 2005;579:2569-75 pubmed
product information
Antigen :
CD31
Apps. Abbrev. :
FC
Cat # :
303109
Clone :
WM59
Item :
Alexa Fluor® 488 anti-human CD31
Isotype :
Mouse IgG1, κ
Other Names :
PECAM-1, EndoCAM
Size :
25 tests
Price (USD) :
124 USD
Reactivity :
Human, Cynomolgus, Rhesus
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
A488
Application Notes :
Clone WM59 has been reported to recognize the D2 extracellular portion of CD31.Additional reported applications (for the relevant formats) include: immunofluorescence microscopy^2, immunohistochemical staining of acetone-fixed frozen tissue sections^8, blocking of platelet aggregation^3, and spatial biology (IBEX)^11,12. Clone WM59 is not recommended for immunohistochemical staining of formalin-fixed paraffin-embedded sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 303143 & 303144).The purified WM59 antibody is useful as a capture antibody for a sandwich ELISA assay, when used in conjunction with biotin anti-human CD31 antibody (Cat. No. 536604) antibody as the detection antibody.;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA