LEAF™ Purified anti-human CD31 | BioLegend 303108 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

LEAF™ Purified anti-human CD31

catalog :

303108

quantity :

500 ug

price :

350 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

WM59

reactivity :

human, rhesus macaque

application :

immunohistochemistry, neutralization, flow cytometry

citations: 12

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; loading ...; fig 9b	Casey A, Sinha A, Singhania R, Livingstone J, Waterhouse P, Tharmapalan P, et al. Mammary molecular portraits reveal lineage-specific features and progenitor cell vulnerabilities. J Cell Biol. 2018;217:2951-2974 pubmed publisher
flow cytometry; human; 1:33; loading ...; fig 3a	Britschgi A, Duss S, Kim S, Couto J, Brinkhaus H, Koren S, et al. The Hippo kinases LATS1 and 2 control human breast cell fate via crosstalk with ERα. Nature. 2017;541:541-545 pubmed publisher
flow cytometry; human	Cordeiro O, Chypre M, Brouard N, Rauber S, Alloush F, Romera Hernandez M, et al. Integrin-Alpha IIb Identifies Murine Lymph Node Lymphatic Endothelial Cells Responsive to RANKL. PLoS ONE. 2016;11:e0151848 pubmed publisher
flow cytometry; human; fig 8	Kaplan J, Marshall M, C McSkimming C, Harmon D, Garmey J, Oldham S, et al. Adipocyte progenitor cells initiate monocyte chemoattractant protein-1-mediated macrophage accumulation in visceral adipose tissue. Mol Metab. 2015;4:779-94 pubmed publisher
flow cytometry; human; fig 4	Rissiek A, Baumann I, Cuapio A, Mautner A, Kolster M, Arck P, et al. The expression of CD39 on regulatory T cells is genetically driven and further upregulated at sites of inflammation. J Autoimmun. 2015;58:12-20 pubmed publisher
flow cytometry; human; fig 2d	Santoro S, Kim S, Motz G, Alatzoglou D, Li C, Irving M, et al. T cells bearing a chimeric antigen receptor against prostate-specific membrane antigen mediate vascular disruption and result in tumor regression. Cancer Immunol Res. 2015;3:68-84 pubmed publisher
flow cytometry; human	Orecchioni S, Gregato G, Martin Padura I, Reggiani F, Braidotti P, Mancuso P, et al. Complementary populations of human adipose CD34+ progenitor cells promote growth, angiogenesis, and metastasis of breast cancer. Cancer Res. 2013;73:5880-91 pubmed publisher
	Bushway M, Gerber S, Fenton B, Miller R, Lord E, Murphy S. Morphological and phenotypic analyses of the human placenta using whole mount immunofluorescence. Biol Reprod. 2014;90:110 pubmed publisher
	Oeztuerk Winder F, Guinot A, Ochalek A, Ventura J. Regulation of human lung alveolar multipotent cells by a novel p38Î± MAPK/miR-17-92 axis. EMBO J. 2012;31:3431-41 pubmed publisher
	Wicki A, Rochlitz C, Orleth A, Ritschard R, Albrecht I, Herrmann R, et al. Targeting tumor-associated endothelial cells: anti-VEGFR2 immunoliposomes mediate tumor vessel disruption and inhibit tumor growth. Clin Cancer Res. 2012;18:454-64 pubmed publisher
	Nagano M, Yamashita T, Hamada H, Ohneda K, Kimura K, Nakagawa T, et al. Identification of functional endothelial progenitor cells suitable for the treatment of ischemic tissue using human umbilical cord blood. Blood. 2007;110:151-60 pubmed
	MacFadyen J, Haworth O, Roberston D, Hardie D, Webster M, Morris H, et al. Endosialin (TEM1, CD248) is a marker of stromal fibroblasts and is not selectively expressed on tumour endothelium. FEBS Lett. 2005;579:2569-75 pubmed

product information

Antigen :

CD31

Apps. Abbrev. :

Flow Cytometry, IHC, Immunofluorescence Microscopy, Block

Cat # :

303108

Clone :

WM59

Item :

LEAF™ Purified anti-human CD31

Isotype :

Mouse IgG1, kappa

Other Names :

PECAM-1, EndoCAM

Size :

500 ug

Price (USD) :

350 USD

Reactivity :

Human, African Green, Baboon, Cynomolgus, Rhesus

Clonality :

Monoclonal

Host :

Mouse

Conjugate/Tag/Label :

LEAF

Application Notes :

. Clone WM59 has been reported to recognize the D2 extracellular portion of CD31. Additional reported applications (for the relevant formats) include: immunofluorescence microscopy 2 , immunohistochemical staining of acetone-fixed frozen tissue sections 8 , and blocking of platelet aggregation 3 . Clone WM59 is not recommended for immunohistochemical staining of formalin-fixed paraffin-embedded sections. The Ultra-LEAF™ purified antibody (Endotoxin 0.01 EU/ug, Azide-Free, 0.2 um filtered) is recommended for functional assays (Cat. No. 303143 303144). The purified WM59 antibody is useful as a capture antibody for a sandwich ELISA assay, when used in conjunction with biotinylated O92E4 (Cat. No. 536604) antibody as the detection antibody. Note: For testing human CD31 in cell culture supernatant, serum, plasma, other biological fluids, BioLegend's LEGEND MAX™ Kits (Cat. No. 443607) are specially developed and recommended.