product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Alexa Fluor® 647 anti-human CD28
catalog :
302953
quantity :
25 tests
price :
124 USD
clonality :
monoclonal
host :
mouse
conjugate :
AF647
clone name :
CD28.2
reactivity :
human, rhesus macaque
application :
flow cytometry, blocking or activating experiments, mass cytometry
more info or order :
citations: 41
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; loading ...; fig 2b
Laffey K, Stiles R, Ludescher M, Davis T, Khwaja S, Bram R, et al. Early expression of mature αβ TCR in CD4-CD8- T cell progenitors enables MHC to drive development of T-ALL bearing NOTCH mutations. Proc Natl Acad Sci U S A. 2022;119:e2118529119 pubmed publisher
  • mass cytometry; human; 1:50; loading ...; fig s2
West J, Austin E, Rizzi E, Yan L, Tanjore H, Crabtree A, et al. KCNK3 Mutation Causes Altered Immune Function in Pulmonary Arterial Hypertension Patients and Mouse Models. Int J Mol Sci. 2021;22: pubmed publisher
  • flow cytometry; human; 1:50; loading ...
Zurli V, Montecchi T, Heilig R, Poschke I, Volkmar M, Wimmer G, et al. Phosphoproteomics of CD2 signaling reveals AMPK-dependent regulation of lytic granule polarization in cytotoxic T cells. Sci Signal. 2020;13: pubmed publisher
  • flow cytometry; human; loading ...; fig 1b
Humeniuk P, Geiselhart S, Battin C, Webb T, Steinberger P, Paster W, et al. Generation of a Jurkat-based fluorescent reporter cell line to evaluate lipid antigen interaction with the human iNKT cell receptor. Sci Rep. 2019;9:7426 pubmed publisher
  • flow cytometry; human; loading ...; fig 3a
Montel Hagen A, Seet C, Li S, Chick B, Zhu Y, Chang P, et al. Organoid-Induced Differentiation of Conventional T Cells from Human Pluripotent Stem Cells. Cell Stem Cell. 2019;24:376-389.e8 pubmed publisher
  • blocking or activating experiments; human; loading ...; fig 1a
Fischer M, Bantug G, Dimeloe S, Gubser P, Burgener A, Grählert J, et al. Early effector maturation of naïve human CD8+ T cells requires mitochondrial biogenesis. Eur J Immunol. 2018;48:1632-1643 pubmed publisher
  • blocking or activating experiments; human; loading ...; fig 2c
Domae E, Hirai Y, Ikeo T, Goda S, Shimizu Y. Cytokine-mediated activation of human ex vivo-expanded V?9V?2 T cells. Oncotarget. 2017;8:45928-45942 pubmed publisher
  • flow cytometry; human; loading ...; fig 4
Heath J, Newhook N, Comeau E, Gallant M, Fudge N, Grant M. NKG2C(+)CD57(+) Natural Killer Cell Expansion Parallels Cytomegalovirus-Specific CD8(+) T Cell Evolution towards Senescence. J Immunol Res. 2016;2016:7470124 pubmed publisher
Agha O, Mueller Immergluck A, Liu M, Zhang H, Theologis A, Clark A, et al. Intervertebral disc herniation effects on multifidus muscle composition and resident stem cell populations. JOR Spine. 2020;3:e1091 pubmed publisher
Thauland T, Pellerin L, Ohgami R, Bacchetta R, Butte M. Case Study: Mechanism for Increased Follicular Helper T Cell Development in Activated PI3K Delta Syndrome. Front Immunol. 2019;10:753 pubmed publisher
Salem M, Wallace C, Velegraki M, Li A, Ansa Addo E, Metelli A, et al. GARP Dampens Cancer Immunity by Sustaining Function and Accumulation of Regulatory T Cells in the Colon. Cancer Res. 2019;: pubmed publisher
OhAinle M, Helms L, Vermeire J, Roesch F, Humes D, Basom R, et al. A virus-packageable CRISPR screen identifies host factors mediating interferon inhibition of HIV. elife. 2018;7: pubmed publisher
Muscate F, Stetter N, Schramm C, Schulze zur Wiesch J, Bosurgi L, Jacobs T. HVEM and CD160: Regulators of Immunopathology During Malaria Blood-Stage. Front Immunol. 2018;9:2611 pubmed publisher
Bray C, Wright D, Haupt S, Thomas S, Stauss H, Zamoyska R. Crispr/Cas Mediated Deletion of PTPN22 in Jurkat T Cells Enhances TCR Signaling and Production of IL-2. Front Immunol. 2018;9:2595 pubmed publisher
Dias J, Boulouis C, Gorin J, van den Biggelaar R, Lal K, Gibbs A, et al. The CD4-CD8- MAIT cell subpopulation is a functionally distinct subset developmentally related to the main CD8+ MAIT cell pool. Proc Natl Acad Sci U S A. 2018;115:E11513-E11522 pubmed publisher
Singh S, Ramirez Salazar E, Doueiri R, Valentin A, Rosati M, Hu X, et al. Control of Heterologous Simian Immunodeficiency Virus SIVsmE660 Infection by DNA and Protein Coimmunization Regimens Combined with Different Toll-Like-Receptor-4-Based Adjuvants in Macaques. J Virol. 2018;92: pubmed publisher
Schmidt A, Marabita F, Kiani N, Gross C, Johansson H, Elias S, et al. Time-resolved transcriptome and proteome landscape of human regulatory T cell (Treg) differentiation reveals novel regulators of FOXP3. BMC Biol. 2018;16:47 pubmed publisher
Laksono B, Grosserichter Wagener C, de Vries R, Langeveld S, Brem M, van Dongen J, et al. In Vitro Measles Virus Infection of Human Lymphocyte Subsets Demonstrates High Susceptibility and Permissiveness of both Naive and Memory B Cells. J Virol. 2018;92: pubmed publisher
Carisey A, Mace E, Saeed M, Davis D, Orange J. Nanoscale Dynamism of Actin Enables Secretory Function in Cytolytic Cells. Curr Biol. 2018;28:489-502.e9 pubmed publisher
Huang S, Ren Y, Thomas A, Chan D, Mueller S, Ward A, et al. Latent HIV reservoirs exhibit inherent resistance to elimination by CD8+ T cells. J Clin Invest. 2018;128:876-889 pubmed publisher
Pawlak E, Dirk B, Jacob R, Johnson A, Dikeakos J. The HIV-1 accessory proteins Nef and Vpu downregulate total and cell surface CD28 in CD4+ T cells. Retrovirology. 2018;15:6 pubmed publisher
Gee M, Han A, Lofgren S, Beausang J, Mendoza J, Birnbaum M, et al. Antigen Identification for Orphan T Cell Receptors Expressed on Tumor-Infiltrating Lymphocytes. Cell. 2018;172:549-563.e16 pubmed publisher
Müller S, Kohanbash G, Liu S, Alvarado B, Carrera D, Bhaduri A, et al. Single-cell profiling of human gliomas reveals macrophage ontogeny as a basis for regional differences in macrophage activation in the tumor microenvironment. Genome Biol. 2017;18:234 pubmed publisher
Ranganath N, Sandstrom T, Burke Schinkel S, Côté S, Angel J. The oncolytic virus, MG1, targets and eliminates latently HIV-1-infected cells: implications for an HIV cure. J Infect Dis. 2017;: pubmed publisher
Ibrahim M, Scozzi D, Toth K, Ponti D, Kreisel D, Menna C, et al. Naive CD4+ T Cells Carrying a TLR2 Agonist Overcome TGF-β-Mediated Tumor Immune Evasion. J Immunol. 2018;200:847-856 pubmed publisher
Callender L, Carroll E, Beal R, Chambers E, Nourshargh S, Akbar A, et al. Human CD8+ EMRA T cells display a senescence-associated secretory phenotype regulated by p38 MAPK. Aging Cell. 2017;: pubmed publisher
Na H, Lim H, Choi G, Kim B, Kim S, Chang Y, et al. Concomitant suppression of TH2 and TH17 cell responses in allergic asthma by targeting retinoic acid receptor-related orphan receptor ?t. J Allergy Clin Immunol. 2018;141:2061-2073.e5 pubmed publisher
Bennett M, Trivedi S, Iyer A, Hale J, Leung D. Human mucosal-associated invariant T (MAIT) cells possess capacity for B cell help. J Leukoc Biol. 2017;102:1261-1269 pubmed publisher
Ge Y, Paisie T, Newman J, McIntyre L, Concannon P. UBASH3A Mediates Risk for Type 1 Diabetes Through Inhibition of T-Cell Receptor-Induced NF-κB Signaling. Diabetes. 2017;66:2033-2043 pubmed publisher
Pattabiraman G, Panchal R, Medvedev A. The R753Q polymorphism in Toll-like receptor 2 (TLR2) attenuates innate immune responses to mycobacteria and impairs MyD88 adapter recruitment to TLR2. J Biol Chem. 2017;292:10685-10695 pubmed publisher
Chiba A, Tamura N, Yoshikiyo K, Murayama G, Kitagaichi M, Yamaji K, et al. Activation status of mucosal-associated invariant T cells reflects disease activity and pathology of systemic lupus erythematosus. Arthritis Res Ther. 2017;19:58 pubmed publisher
Kooy Winkelaar Y, Bouwer D, Janssen G, Thompson A, Brugman M, Schmitz F, et al. CD4 T-cell cytokines synergize to induce proliferation of malignant and nonmalignant innate intraepithelial lymphocytes. Proc Natl Acad Sci U S A. 2017;114:E980-E989 pubmed publisher
Pangrazzi L, Meryk A, Naismith E, Koziel R, Lair J, Krismer M, et al. "Inflamm-aging" influences immune cell survival factors in human bone marrow. Eur J Immunol. 2017;47:481-492 pubmed publisher
Margraf S, Garner L, Wilson T, Brown M. A polymorphism in a phosphotyrosine signalling motif of CD229 (Ly9, SLAMF3) alters SH2 domain binding and T-cell activation. Immunology. 2015;146:392-400 pubmed publisher
Nomura T, Yamamoto H, Shiino T, Takahashi N, Nakane T, Iwamoto N, et al. Association of major histocompatibility complex class I haplotypes with disease progression after simian immunodeficiency virus challenge in burmese rhesus macaques. J Virol. 2012;86:6481-90 pubmed publisher
Leonard J, Filzen T, Carter C, Schaefer M, Collins K. HIV-1 Nef disrupts intracellular trafficking of major histocompatibility complex class I, CD4, CD8, and CD28 by distinct pathways that share common elements. J Virol. 2011;85:6867-81 pubmed publisher
Ye Z, Lee C, Sun G, Gan Y. Burkholderia pseudomallei infection of T cells leads to T-cell costimulation partially provided by flagellin. Infect Immun. 2008;76:2541-50 pubmed publisher
Berg M, Zavazava N. Regulation of CD28 expression on CD8+ T cells by CTLA-4. J Leukoc Biol. 2008;83:853-63 pubmed
Magatti M, De Munari S, Vertua E, Gibelli L, Wengler G, Parolini O. Human amnion mesenchyme harbors cells with allogeneic T-cell suppression and stimulation capabilities. Stem Cells. 2008;26:182-92 pubmed
Mitsuhashi M. Ex vivo simulation of drug action: quantification of drug-induced mRNA as a bridge between preclinical and clinical trials. Clin Chem. 2007;53:148-9 pubmed
Sheng W, Chen Y, Wang T. A major role of PKC theta and NFkappaB in the regulation of hTERT in human T lymphocytes. FEBS Lett. 2006;580:6819-24 pubmed
product information
Antigen :
CD28
Apps. Abbrev. :
FC
Cat # :
302953
Clone :
CD28.2
Item :
Alexa Fluor® 647 anti-human CD28
Isotype :
Mouse IgG1, κ
Other Names :
T44, Tp44
Size :
25 tests
Price (USD) :
124 USD
Reactivity :
Human, Cynomolgus, Rhesus
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
A647
Application Notes :
The Ultra-LEAF™ Purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for highly sensitive assays.;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA