product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Ultra-LEAF™ Purified anti-human CD28
catalog :
302933
quantity :
100 μg
price :
110 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
CD28.2
reactivity :
human, rhesus macaque
application :
flow cytometry, immunohistochemistry - frozen section, blocking or activating experiments, mass cytometry
more info or order :
citations: 82
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; loading ...; fig 2b
Laffey K, Stiles R, Ludescher M, Davis T, Khwaja S, Bram R, et al. Early expression of mature αβ TCR in CD4-CD8- T cell progenitors enables MHC to drive development of T-ALL bearing NOTCH mutations. Proc Natl Acad Sci U S A. 2022;119:e2118529119 pubmed publisher
  • mass cytometry; human; 1:50; loading ...; fig s2
West J, Austin E, Rizzi E, Yan L, Tanjore H, Crabtree A, et al. KCNK3 Mutation Causes Altered Immune Function in Pulmonary Arterial Hypertension Patients and Mouse Models. Int J Mol Sci. 2021;22: pubmed publisher
  • flow cytometry; human; 1:50; loading ...
Zurli V, Montecchi T, Heilig R, Poschke I, Volkmar M, Wimmer G, et al. Phosphoproteomics of CD2 signaling reveals AMPK-dependent regulation of lytic granule polarization in cytotoxic T cells. Sci Signal. 2020;13: pubmed publisher
  • flow cytometry; human; loading ...; fig 1b
Humeniuk P, Geiselhart S, Battin C, Webb T, Steinberger P, Paster W, et al. Generation of a Jurkat-based fluorescent reporter cell line to evaluate lipid antigen interaction with the human iNKT cell receptor. Sci Rep. 2019;9:7426 pubmed publisher
  • flow cytometry; human; loading ...; fig 3a
Montel Hagen A, Seet C, Li S, Chick B, Zhu Y, Chang P, et al. Organoid-Induced Differentiation of Conventional T Cells from Human Pluripotent Stem Cells. Cell Stem Cell. 2019;24:376-389.e8 pubmed publisher
  • blocking or activating experiments; human; loading ...; fig 1a
Fischer M, Bantug G, Dimeloe S, Gubser P, Burgener A, Grählert J, et al. Early effector maturation of naïve human CD8+ T cells requires mitochondrial biogenesis. Eur J Immunol. 2018;48:1632-1643 pubmed publisher
  • blocking or activating experiments; human; loading ...; fig 2c
Domae E, Hirai Y, Ikeo T, Goda S, Shimizu Y. Cytokine-mediated activation of human ex vivo-expanded V?9V?2 T cells. Oncotarget. 2017;8:45928-45942 pubmed publisher
  • flow cytometry; human
Gaggianesi M, Turdo A, Chinnici A, Lipari E, Apuzzo T, Benfante A, et al. IL4 Primes the Dynamics of Breast Cancer Progression via DUSP4 Inhibition. Cancer Res. 2017;77:3268-3279 pubmed publisher
  • flow cytometry; rhesus macaque; loading ...; fig 4b
Mylvaganam G, Rios D, Abdelaal H, Iyer S, Tharp G, Mavigner M, et al. Dynamics of SIV-specific CXCR5+ CD8 T cells during chronic SIV infection. Proc Natl Acad Sci U S A. 2017;114:1976-1981 pubmed publisher
  • flow cytometry; human; loading ...; fig s6b
Kinosada H, Yasunaga J, Shimura K, Miyazato P, Onishi C, Iyoda T, et al. HTLV-1 bZIP Factor Enhances T-Cell Proliferation by Impeding the Suppressive Signaling of Co-inhibitory Receptors. PLoS Pathog. 2017;13:e1006120 pubmed publisher
  • blocking or activating experiments; human; loading ...; fig s4a
Serr I, Fürst R, Ott V, Scherm M, Nikolaev A, Gökmen F, et al. miRNA92a targets KLF2 and the phosphatase PTEN signaling to promote human T follicular helper precursors in T1D islet autoimmunity. Proc Natl Acad Sci U S A. 2016;113:E6659-E6668 pubmed
  • flow cytometry; rhesus macaque; fig 4a
Hu X, Valentin A, Dayton F, Kulkarni V, Alicea C, Rosati M, et al. DNA Prime-Boost Vaccine Regimen To Increase Breadth, Magnitude, and Cytotoxicity of the Cellular Immune Responses to Subdominant Gag Epitopes of Simian Immunodeficiency Virus and HIV. J Immunol. 2016;197:3999-4013 pubmed
  • blocking or activating experiments; human; loading ...
Jung Y, Riven I, Feigelson S, Kartvelishvily E, Tohya K, Miyasaka M, et al. Three-dimensional localization of T-cell receptors in relation to microvilli using a combination of superresolution microscopies. Proc Natl Acad Sci U S A. 2016;113:E5916-E5924 pubmed
  • flow cytometry; human; fig s1
Pachnio A, Ciáurriz M, Begum J, Lal N, Zuo J, Beggs A, et al. Cytomegalovirus Infection Leads to Development of High Frequencies of Cytotoxic Virus-Specific CD4+ T Cells Targeted to Vascular Endothelium. PLoS Pathog. 2016;12:e1005832 pubmed publisher
  • flow cytometry; human; loading ...; fig 4
Heath J, Newhook N, Comeau E, Gallant M, Fudge N, Grant M. NKG2C(+)CD57(+) Natural Killer Cell Expansion Parallels Cytomegalovirus-Specific CD8(+) T Cell Evolution towards Senescence. J Immunol Res. 2016;2016:7470124 pubmed publisher
  • blocking or activating experiments; human; loading ...
Ma Q, Garber H, Lu S, He H, Tallis E, Ding X, et al. A novel TCR-like CAR with specificity for PR1/HLA-A2 effectively targets myeloid leukemia in vitro when expressed in human adult peripheral blood and cord blood T cells. Cytotherapy. 2016;18:985-94 pubmed publisher
  • flow cytometry; human; fig 1
van der Heiden M, van Zelm M, Bartol S, de Rond L, Berbers G, Boots A, et al. Differential effects of Cytomegalovirus carriage on the immune phenotype of middle-aged males and females. Sci Rep. 2016;6:26892 pubmed publisher
  • blocking or activating experiments; human; fig 3
Jutz S, Leitner J, Schmetterer K, Doel Perez I, Majdic O, Grabmeier Pfistershammer K, et al. Assessment of costimulation and coinhibition in a triple parameter T cell reporter line: Simultaneous measurement of NF-κB, NFAT and AP-1. J Immunol Methods. 2016;430:10-20 pubmed publisher
  • flow cytometry; human; fig 1
Frederiksen J, Buggert M, Noyan K, Nowak P, Sönnerborg A, Lund O, et al. Multidimensional Clusters of CD4+ T Cell Dysfunction Are Primarily Associated with the CD4/CD8 Ratio in Chronic HIV Infection. PLoS ONE. 2015;10:e0137635 pubmed publisher
  • blocking or activating experiments; human; fig 7
Patsoukis N, Bardhan K, Chatterjee P, Sari D, Liu B, Bell L, et al. PD-1 alters T-cell metabolic reprogramming by inhibiting glycolysis and promoting lipolysis and fatty acid oxidation. Nat Commun. 2015;6:6692 pubmed publisher
  • blocking or activating experiments; human; fig 6
Li J, Jie H, Lei Y, Gildener Leapman N, Trivedi S, Green T, et al. PD-1/SHP-2 inhibits Tc1/Th1 phenotypic responses and the activation of T cells in the tumor microenvironment. Cancer Res. 2015;75:508-518 pubmed publisher
  • blocking or activating experiments; human
Lugassy J, Corso J, Beach D, Petrik T, Oellerich T, Urlaub H, et al. Modulation of TCR responsiveness by the Grb2-family adaptor, Gads. Cell Signal. 2015;27:125-34 pubmed publisher
  • flow cytometry; human; fig 3
Valentin A, McKinnon K, Li J, Rosati M, Kulkarni V, Pilkington G, et al. Comparative analysis of SIV-specific cellular immune responses induced by different vaccine platforms in rhesus macaques. Clin Immunol. 2014;155:91-107 pubmed publisher
  • blocking or activating experiments; human
Steiner S, Daniel C, Fischer A, Atreya I, Hirschmann S, Waldner M, et al. Cyclosporine A regulates pro-inflammatory cytokine production in ulcerative colitis. Arch Immunol Ther Exp (Warsz). 2015;63:53-63 pubmed publisher
  • flow cytometry; rhesus macaque
Frencher J, Shen H, Yan L, Wilson J, Freitag N, Rizzo A, et al. HMBPP-deficient Listeria mutant immunization alters pulmonary/systemic responses, effector functions, and memory polarization of Vγ2Vδ2 T cells. J Leukoc Biol. 2014;96:957-67 pubmed publisher
  • flow cytometry; human; 1 ug/ml
Weist B, Schmueck M, Fuehrer H, Sattler A, Reinke P, Babel N. The role of CD4(+) T cells in BKV-specific T cell immunity. Med Microbiol Immunol. 2014;203:395-408 pubmed publisher
  • flow cytometry; rhesus macaque
Kulkarni V, Valentin A, Rosati M, Alicea C, Singh A, Jalah R, et al. Altered response hierarchy and increased T-cell breadth upon HIV-1 conserved element DNA vaccination in macaques. PLoS ONE. 2014;9:e86254 pubmed publisher
Wen T, Barham W, Li Y, Zhang H, Gicobi J, Hirdler J, et al. NKG7 Is a T-cell-Intrinsic Therapeutic Target for Improving Antitumor Cytotoxicity and Cancer Immunotherapy. Cancer Immunol Res. 2022;10:162-181 pubmed publisher
Jiang J, Yan S, Zhou X, Zhou J, Bai X, Tan Q, et al. Crosstalk Between Circulating Follicular T Helper Cells and Regulatory B Cells in Children With Extrinsic Atopic Dermatitis. Front Immunol. 2021;12:785549 pubmed publisher
Wang C, Li Y, Jia L, Kim J, Li J, Deng P, et al. CD276 expression enables squamous cell carcinoma stem cells to evade immune surveillance. Cell Stem Cell. 2021;28:1597-1613.e7 pubmed publisher
Martínez Zamudio R, Dewald H, Vasilopoulos T, Gittens Williams L, Fitzgerald Bocarsly P, Herbig U. Senescence-associated β-galactosidase reveals the abundance of senescent CD8+ T cells in aging humans. Aging Cell. 2021;20:e13344 pubmed publisher
Glassman C, Mathiharan Y, Jude K, Su L, Panova O, Lupardus P, et al. Structural basis for IL-12 and IL-23 receptor sharing reveals a gateway for shaping actions on T versus NK cells. Cell. 2021;184:983-999.e24 pubmed publisher
Clayton K, Mylvaganam G, Villasmil Ocando A, Stuart H, Maus M, Rashidian M, et al. HIV-infected macrophages resist efficient NK cell-mediated killing while preserving inflammatory cytokine responses. Cell Host Microbe. 2021;29:435-447.e9 pubmed publisher
Srivastava S, Furlan S, Jaeger Ruckstuhl C, Sarvothama M, Berger C, Smythe K, et al. Immunogenic Chemotherapy Enhances Recruitment of CAR-T Cells to Lung Tumors and Improves Antitumor Efficacy when Combined with Checkpoint Blockade. Cancer Cell. 2021;39:193-208.e10 pubmed publisher
Kim S, MacDonald J, Dick F. Phosphorylation of the RB C-terminus regulates condensin II release from chromatin. J Biol Chem. 2021;296:100108 pubmed publisher
Saragovi A, Abramovich I, Omar I, Arbib E, Toker O, Gottlieb E, et al. Systemic hypoxia inhibits T cell response by limiting mitobiogenesis via matrix substrate-level phosphorylation arrest. elife. 2020;9: pubmed publisher
Si J, Shi X, Sun S, Zou B, Li Y, An D, et al. Hematopoietic Progenitor Kinase1 (HPK1) Mediates T Cell Dysfunction and Is a Druggable Target for T Cell-Based Immunotherapies. Cancer Cell. 2020;38:551-566.e11 pubmed publisher
Hoekstra M, Bornes L, Dijkgraaf F, Philips D, Pardieck I, Toebes M, et al. Long-distance modulation of bystander tumor cells by CD8+ T cell-secreted IFNγ. Nat Cancer. 2020;1:291-301 pubmed publisher
McBrien J, Mavigner M, Franchitti L, Smith S, White E, Tharp G, et al. Robust and persistent reactivation of SIV and HIV by N-803 and depletion of CD8+ cells. Nature. 2020;578:154-159 pubmed publisher
Callender L, Carroll E, Bober E, Akbar A, Solito E, Henson S. Mitochondrial mass governs the extent of human T cell senescence. Aging Cell. 2020;19:e13067 pubmed publisher
Grandclaudon M, Perrot Dockès M, Trichot C, Karpf L, Abouzid O, Chauvin C, et al. A Quantitative Multivariate Model of Human Dendritic Cell-T Helper Cell Communication. Cell. 2019;179:432-447.e21 pubmed publisher
Sun T, Ju M, Dai X, Dong H, Gu W, Gao Y, et al. Multilevel defects in the hematopoietic niche in essential thrombocythemia. Haematologica. 2020;105:661-673 pubmed publisher
Celis Gutierrez J, Blattmann P, Zhai Y, Jarmuzynski N, Ruminski K, Gregoire C, et al. Quantitative Interactomics in Primary T Cells Provides a Rationale for Concomitant PD-1 and BTLA Coinhibitor Blockade in Cancer Immunotherapy. Cell Rep. 2019;27:3315-3330.e7 pubmed publisher
Thauland T, Pellerin L, Ohgami R, Bacchetta R, Butte M. Case Study: Mechanism for Increased Follicular Helper T Cell Development in Activated PI3K Delta Syndrome. Front Immunol. 2019;10:753 pubmed publisher
Wang W, Chapman N, Zhang B, Li M, Fan M, Laribee R, et al. Upregulation of PD-L1 via HMGB1-Activated IRF3 and NF-κB Contributes to UV Radiation-Induced Immune Suppression. Cancer Res. 2019;79:2909-2922 pubmed publisher
Salem M, Wallace C, Velegraki M, Li A, Ansa Addo E, Metelli A, et al. GARP Dampens Cancer Immunity by Sustaining Function and Accumulation of Regulatory T Cells in the Colon. Cancer Res. 2019;: pubmed publisher
OhAinle M, Helms L, Vermeire J, Roesch F, Humes D, Basom R, et al. A virus-packageable CRISPR screen identifies host factors mediating interferon inhibition of HIV. elife. 2018;7: pubmed publisher
Muscate F, Stetter N, Schramm C, Schulze zur Wiesch J, Bosurgi L, Jacobs T. HVEM and CD160: Regulators of Immunopathology During Malaria Blood-Stage. Front Immunol. 2018;9:2611 pubmed publisher
Bray C, Wright D, Haupt S, Thomas S, Stauss H, Zamoyska R. Crispr/Cas Mediated Deletion of PTPN22 in Jurkat T Cells Enhances TCR Signaling and Production of IL-2. Front Immunol. 2018;9:2595 pubmed publisher
Dias J, Boulouis C, Gorin J, van den Biggelaar R, Lal K, Gibbs A, et al. The CD4-CD8- MAIT cell subpopulation is a functionally distinct subset developmentally related to the main CD8+ MAIT cell pool. Proc Natl Acad Sci U S A. 2018;115:E11513-E11522 pubmed publisher
Wang T, Fan C, Yao A, Xu X, Zheng G, You Y, et al. The Adaptor Protein CARD9 Protects against Colon Cancer by Restricting Mycobiota-Mediated Expansion of Myeloid-Derived Suppressor Cells. Immunity. 2018;49:504-514.e4 pubmed publisher
Zhang A, Yoon J, Kim Y, Scott D. Targeting Antigen-Specific B Cells Using Antigen-Expressing Transduced Regulatory T Cells. J Immunol. 2018;201:1434-1441 pubmed publisher
Singh S, Ramirez Salazar E, Doueiri R, Valentin A, Rosati M, Hu X, et al. Control of Heterologous Simian Immunodeficiency Virus SIVsmE660 Infection by DNA and Protein Coimmunization Regimens Combined with Different Toll-Like-Receptor-4-Based Adjuvants in Macaques. J Virol. 2018;92: pubmed publisher
Schmidt A, Marabita F, Kiani N, Gross C, Johansson H, Elias S, et al. Time-resolved transcriptome and proteome landscape of human regulatory T cell (Treg) differentiation reveals novel regulators of FOXP3. BMC Biol. 2018;16:47 pubmed publisher
Correnti C, Laszlo G, de van der Schueren W, Godwin C, Bandaranayake A, Busch M, et al. Simultaneous multiple interaction T-cell engaging (SMITE) bispecific antibodies overcome bispecific T-cell engager (BiTE) resistance via CD28 co-stimulation. Leukemia. 2018;32:1239-1243 pubmed publisher
Laksono B, Grosserichter Wagener C, de Vries R, Langeveld S, Brem M, van Dongen J, et al. In Vitro Measles Virus Infection of Human Lymphocyte Subsets Demonstrates High Susceptibility and Permissiveness of both Naive and Memory B Cells. J Virol. 2018;92: pubmed publisher
Carisey A, Mace E, Saeed M, Davis D, Orange J. Nanoscale Dynamism of Actin Enables Secretory Function in Cytolytic Cells. Curr Biol. 2018;28:489-502.e9 pubmed publisher
Huang S, Ren Y, Thomas A, Chan D, Mueller S, Ward A, et al. Latent HIV reservoirs exhibit inherent resistance to elimination by CD8+ T cells. J Clin Invest. 2018;128:876-889 pubmed publisher
Pawlak E, Dirk B, Jacob R, Johnson A, Dikeakos J. The HIV-1 accessory proteins Nef and Vpu downregulate total and cell surface CD28 in CD4+ T cells. Retrovirology. 2018;15:6 pubmed publisher
Gee M, Han A, Lofgren S, Beausang J, Mendoza J, Birnbaum M, et al. Antigen Identification for Orphan T Cell Receptors Expressed on Tumor-Infiltrating Lymphocytes. Cell. 2018;172:549-563.e16 pubmed publisher
Müller S, Kohanbash G, Liu S, Alvarado B, Carrera D, Bhaduri A, et al. Single-cell profiling of human gliomas reveals macrophage ontogeny as a basis for regional differences in macrophage activation in the tumor microenvironment. Genome Biol. 2017;18:234 pubmed publisher
Ranganath N, Sandstrom T, Burke Schinkel S, Côté S, Angel J. The oncolytic virus, MG1, targets and eliminates latently HIV-1-infected cells: implications for an HIV cure. J Infect Dis. 2017;: pubmed publisher
Ibrahim M, Scozzi D, Toth K, Ponti D, Kreisel D, Menna C, et al. Naive CD4+ T Cells Carrying a TLR2 Agonist Overcome TGF-β-Mediated Tumor Immune Evasion. J Immunol. 2018;200:847-856 pubmed publisher
Callender L, Carroll E, Beal R, Chambers E, Nourshargh S, Akbar A, et al. Human CD8+ EMRA T cells display a senescence-associated secretory phenotype regulated by p38 MAPK. Aging Cell. 2017;: pubmed publisher
Na H, Lim H, Choi G, Kim B, Kim S, Chang Y, et al. Concomitant suppression of TH2 and TH17 cell responses in allergic asthma by targeting retinoic acid receptor-related orphan receptor ?t. J Allergy Clin Immunol. 2018;141:2061-2073.e5 pubmed publisher
Bennett M, Trivedi S, Iyer A, Hale J, Leung D. Human mucosal-associated invariant T (MAIT) cells possess capacity for B cell help. J Leukoc Biol. 2017;102:1261-1269 pubmed publisher
Cobb D, Kim O, Golden Mason L, Rosen H, Hahn Y. Hepatocyte-derived exosomes promote T follicular regulatory cell expansion during hepatitis C virus infection. Hepatology. 2017;: pubmed publisher
Ge Y, Paisie T, Newman J, McIntyre L, Concannon P. UBASH3A Mediates Risk for Type 1 Diabetes Through Inhibition of T-Cell Receptor-Induced NF-κB Signaling. Diabetes. 2017;66:2033-2043 pubmed publisher
Pattabiraman G, Panchal R, Medvedev A. The R753Q polymorphism in Toll-like receptor 2 (TLR2) attenuates innate immune responses to mycobacteria and impairs MyD88 adapter recruitment to TLR2. J Biol Chem. 2017;292:10685-10695 pubmed publisher
Chiba A, Tamura N, Yoshikiyo K, Murayama G, Kitagaichi M, Yamaji K, et al. Activation status of mucosal-associated invariant T cells reflects disease activity and pathology of systemic lupus erythematosus. Arthritis Res Ther. 2017;19:58 pubmed publisher
Kooy Winkelaar Y, Bouwer D, Janssen G, Thompson A, Brugman M, Schmitz F, et al. CD4 T-cell cytokines synergize to induce proliferation of malignant and nonmalignant innate intraepithelial lymphocytes. Proc Natl Acad Sci U S A. 2017;114:E980-E989 pubmed publisher
Pangrazzi L, Meryk A, Naismith E, Koziel R, Lair J, Krismer M, et al. "Inflamm-aging" influences immune cell survival factors in human bone marrow. Eur J Immunol. 2017;47:481-492 pubmed publisher
Blewett M, Xie J, Zaro B, Backus K, Altman A, Teijaro J, et al. Chemical proteomic map of dimethyl fumarate-sensitive cysteines in primary human T cells. Sci Signal. 2016;9:rs10 pubmed publisher
Margraf S, Garner L, Wilson T, Brown M. A polymorphism in a phosphotyrosine signalling motif of CD229 (Ly9, SLAMF3) alters SH2 domain binding and T-cell activation. Immunology. 2015;146:392-400 pubmed publisher
Lin M, Yuan Y, Xu J, Cai X, Liu S, Niu L, et al. Safety and efficacy study of nasopharyngeal cancer stem cell vaccine. Immunol Lett. 2015;165:26-31 pubmed publisher
Nomura T, Yamamoto H, Shiino T, Takahashi N, Nakane T, Iwamoto N, et al. Association of major histocompatibility complex class I haplotypes with disease progression after simian immunodeficiency virus challenge in burmese rhesus macaques. J Virol. 2012;86:6481-90 pubmed publisher
Leonard J, Filzen T, Carter C, Schaefer M, Collins K. HIV-1 Nef disrupts intracellular trafficking of major histocompatibility complex class I, CD4, CD8, and CD28 by distinct pathways that share common elements. J Virol. 2011;85:6867-81 pubmed publisher
Ye Z, Lee C, Sun G, Gan Y. Burkholderia pseudomallei infection of T cells leads to T-cell costimulation partially provided by flagellin. Infect Immun. 2008;76:2541-50 pubmed publisher
Berg M, Zavazava N. Regulation of CD28 expression on CD8+ T cells by CTLA-4. J Leukoc Biol. 2008;83:853-63 pubmed
Magatti M, De Munari S, Vertua E, Gibelli L, Wengler G, Parolini O. Human amnion mesenchyme harbors cells with allogeneic T-cell suppression and stimulation capabilities. Stem Cells. 2008;26:182-92 pubmed
Mitsuhashi M. Ex vivo simulation of drug action: quantification of drug-induced mRNA as a bridge between preclinical and clinical trials. Clin Chem. 2007;53:148-9 pubmed
Sheng W, Chen Y, Wang T. A major role of PKC theta and NFkappaB in the regulation of hTERT in human T lymphocytes. FEBS Lett. 2006;580:6819-24 pubmed
product information
Antigen :
CD28
Apps. Abbrev. :
FC, IHC-F, Costim, FA
Cat # :
302933
Clone :
CD28.2
Item :
Ultra-LEAF™ Purified anti-human CD28
Isotype :
Mouse IgG1, κ
Other Names :
T44, Tp44
Size :
100 μg
Price (USD) :
110 USD
Reactivity :
Human, Cynomolgus, Rhesus
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
ULEAF
Application Notes :
The Ultra-LEAF™ Purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for highly sensitive assays.;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA