LEAF™ Purified anti-human CD28 | BioLegend 302923 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

LEAF™ Purified anti-human CD28

catalog :

302923

quantity :

1 mg

price :

245 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

CD28.2

reactivity :

human, chimpanzee, Rhesus monkey

application :

immunohistochemistry, immunoprecipitation, flow cytometry, blocking or activating experiments

citations: 24

Published Application/Species/Sample/Dilution	Reference
blocking or activating experiments; human; loading ...; fig 1a	Fischer M, Bantug G, Dimeloe S, Gubser P, Burgener A, Grählert J, et al. Early effector maturation of naïve human CD8+ T cells requires mitochondrial biogenesis. Eur J Immunol. 2018;48:1632-1643 pubmed publisher
blocking or activating experiments; human; loading ...; fig 2c	Domae E, Hirai Y, Ikeo T, Goda S, Shimizu Y. Cytokine-mediated activation of human ex vivo-expanded V?9V?2 T cells. Oncotarget. 2017;8:45928-45942 pubmed publisher
flow cytometry; human	Gaggianesi M, Turdo A, Chinnici A, Lipari E, Apuzzo T, Benfante A, et al. IL4 Primes the Dynamics of Breast Cancer Progression via DUSP4 Inhibition. Cancer Res. 2017;77:3268-3279 pubmed publisher
flow cytometry; Rhesus monkey; loading ...; fig 4b	Mylvaganam G, Rios D, Abdelaal H, Iyer S, Tharp G, Mavigner M, et al. Dynamics of SIV-specific CXCR5+ CD8 T cells during chronic SIV infection. Proc Natl Acad Sci U S A. 2017;114:1976-1981 pubmed publisher
flow cytometry; human; loading ...; fig s6b	Kinosada H, Yasunaga J, Shimura K, Miyazato P, Onishi C, Iyoda T, et al. HTLV-1 bZIP Factor Enhances T-Cell Proliferation by Impeding the Suppressive Signaling of Co-inhibitory Receptors. PLoS Pathog. 2017;13:e1006120 pubmed publisher
blocking or activating experiments; human; loading ...; fig s4a	Serr I, FÃ¼rst R, Ott V, Scherm M, Nikolaev A, GÃ¶kmen F, et al. miRNA92a targets KLF2 and the phosphatase PTEN signaling to promote human T follicular helper precursors in T1D islet autoimmunity. Proc Natl Acad Sci U S A. 2016;113:E6659-E6668 pubmed
flow cytometry; Rhesus monkey; fig 4a	Hu X, Valentin A, Dayton F, Kulkarni V, Alicea C, Rosati M, et al. DNA Prime-Boost Vaccine Regimen To Increase Breadth, Magnitude, and Cytotoxicity of the Cellular Immune Responses to Subdominant Gag Epitopes of Simian Immunodeficiency Virus and HIV. J Immunol. 2016;197:3999-4013 pubmed
blocking or activating experiments; human; loading ...	Jung Y, Riven I, Feigelson S, Kartvelishvily E, Tohya K, Miyasaka M, et al. Three-dimensional localization of T-cell receptors in relation to microvilli using a combination of superresolution microscopies. Proc Natl Acad Sci U S A. 2016;113:E5916-E5924 pubmed
flow cytometry; human; fig s1	Pachnio A, Ciáurriz M, Begum J, Lal N, Zuo J, Beggs A, et al. Cytomegalovirus Infection Leads to Development of High Frequencies of Cytotoxic Virus-Specific CD4+ T Cells Targeted to Vascular Endothelium. PLoS Pathog. 2016;12:e1005832 pubmed publisher
ELISA; human; 2.5 ug/ml; loading ...	Guillou L, Babataheri A, Saitakis M, Bohineust A, Dogniaux S, Hivroz C, et al. T-lymphocyte passive deformation is controlled by unfolding of membrane surface reservoirs. Mol Biol Cell. 2016;27:3574-3582 pubmed
flow cytometry; human; loading ...; fig 4	Heath J, Newhook N, Comeau E, Gallant M, Fudge N, Grant M. NKG2C(+)CD57(+) Natural Killer Cell Expansion Parallels Cytomegalovirus-Specific CD8(+) T Cell Evolution towards Senescence. J Immunol Res. 2016;2016:7470124 pubmed publisher
blocking or activating experiments; human; loading ...	Ma Q, Garber H, Lu S, He H, Tallis E, Ding X, et al. A novel TCR-like CAR with specificity for PR1/HLA-A2 effectively targets myeloid leukemia in vitro when expressed in human adult peripheral blood and cord blood T cells. Cytotherapy. 2016;18:985-94 pubmed publisher
flow cytometry; human; fig 1	van der Heiden M, van Zelm M, Bartol S, de Rond L, Berbers G, Boots A, et al. Differential effects of Cytomegalovirus carriage on the immune phenotype of middle-aged males and females. Sci Rep. 2016;6:26892 pubmed publisher
blocking or activating experiments; human; fig 3	Jutz S, Leitner J, Schmetterer K, Doel Perez I, Majdic O, Grabmeier Pfistershammer K, et al. Assessment of costimulation and coinhibition in a triple parameter T cell reporter line: Simultaneous measurement of NF-ÎºB, NFAT and AP-1. J Immunol Methods. 2016;430:10-20 pubmed publisher
flow cytometry; human; fig 1	Frederiksen J, Buggert M, Noyan K, Nowak P, SÃ¶nnerborg A, Lund O, et al. Multidimensional Clusters of CD4+ T Cell Dysfunction Are Primarily Associated with the CD4/CD8 Ratio in Chronic HIV Infection. PLoS ONE. 2015;10:e0137635 pubmed publisher
blocking or activating experiments; human; fig 7	Patsoukis N, Bardhan K, Chatterjee P, Sari D, Liu B, Bell L, et al. PD-1 alters T-cell metabolic reprogramming by inhibiting glycolysis and promoting lipolysis and fatty acid oxidation. Nat Commun. 2015;6:6692 pubmed publisher
blocking or activating experiments; human; fig 6	Li J, Jie H, Lei Y, Gildener Leapman N, Trivedi S, Green T, et al. PD-1/SHP-2 inhibits Tc1/Th1 phenotypic responses and the activation of T cells in the tumor microenvironment. Cancer Res. 2015;75:508-518 pubmed publisher
blocking or activating experiments; human	Lugassy J, Corso J, Beach D, Petrik T, Oellerich T, Urlaub H, et al. Modulation of TCR responsiveness by the Grb2-family adaptor, Gads. Cell Signal. 2015;27:125-34 pubmed publisher
flow cytometry; human; fig 3	Valentin A, McKinnon K, Li J, Rosati M, Kulkarni V, Pilkington G, et al. Comparative analysis of SIV-specific cellular immune responses induced by different vaccine platforms in rhesus macaques. Clin Immunol. 2014;155:91-107 pubmed publisher
blocking or activating experiments; human	Steiner S, Daniel C, Fischer A, Atreya I, Hirschmann S, Waldner M, et al. Cyclosporine A regulates pro-inflammatory cytokine production in ulcerative colitis. Arch Immunol Ther Exp (Warsz). 2015;63:53-63 pubmed publisher
flow cytometry; Rhesus monkey	Frencher J, Shen H, Yan L, Wilson J, Freitag N, Rizzo A, et al. HMBPP-deficient Listeria mutant immunization alters pulmonary/systemic responses, effector functions, and memory polarization of VÎ³2VÎ´2 T cells. J Leukoc Biol. 2014;96:957-67 pubmed publisher
flow cytometry; human; 1 ug/ml	Weist B, Schmueck M, Fuehrer H, Sattler A, Reinke P, Babel N. The role of CD4(+) T cells in BKV-specific T cell immunity. Med Microbiol Immunol. 2014;203:395-408 pubmed publisher
flow cytometry; Rhesus monkey	Kulkarni V, Valentin A, Rosati M, Alicea C, Singh A, Jalah R, et al. Altered response hierarchy and increased T-cell breadth upon HIV-1 conserved element DNA vaccination in macaques. PLoS ONE. 2014;9:e86254 pubmed publisher
	Klein Geltink R, O Sullivan D, Corrado M, Bremser A, Buck M, Buescher J, et al. Mitochondrial Priming by CD28. Cell. 2017;171:385-397.e11 pubmed publisher

product information

Antigen :

CD28

Apps. Abbrev. :

Flow Cytometry, Costim, IP, IHC

Cat # :

302923

Clone :

CD28.2

Item :

LEAF™ Purified anti-human CD28

Isotype :

Mouse IgG1, κ

Other Names :

T44, Tp44

Size :

1 mg

Price (USD) :

245 USD

Reactivity :

Human, Baboon, Capuchin Monkey, Chimpanzee, Cynomolgus, Pigtailed Macaque, Rhesus, Sooty Mangabey, Squirrel Monkey

Clonality :

Monoclonal

Host :

Mouse

Conjugate/Tag/Label :

LEAF

Application Notes :

Additional reported applications (for the relevant formats) include: immunoprecipitation, immunohistochemical staining of acetone-fixed frozen tissue sections 4 , and in vitro T cell costimulation 5-8 . This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The CD28.2 antibody co-stimulates T cell proliferation and cytokine production in the presence of suboptimal amounts of anti-CD3 antibody. The LEAF purified antibody (Endotoxin 0.1 EU/ug, Azide-Free, 0.2 um filtered) is recommended for functional assays (Cat. No. 302914). For highly sensitive assays, we recommend Ultra-LEAF purified antibody (Cat. No. 302934) with a lower endotoxin limit than standard LEAF purified antibodies (Endotoxin 0.01 EU/ug).