LEAF™ Purified anti-human CD28 | BioLegend 302914 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

LEAF™ Purified anti-human CD28

catalog :

302914

quantity :

500 μg

price :

145 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

CD28.2

reactivity :

human, chimpanzee, Rhesus monkey

application :

immunohistochemistry, immunoprecipitation, flow cytometry, blocking or activating experiments

citations: 24

Published Application/Species/Sample/Dilution	Reference
blocking or activating experiments; human; loading ...; fig 1a	Fischer M, Bantug G, Dimeloe S, Gubser P, Burgener A, Grählert J, et al. Early effector maturation of naïve human CD8+ T cells requires mitochondrial biogenesis. Eur J Immunol. 2018;48:1632-1643 pubmed publisher
blocking or activating experiments; human; loading ...; fig 2c	Domae E, Hirai Y, Ikeo T, Goda S, Shimizu Y. Cytokine-mediated activation of human ex vivo-expanded V?9V?2 T cells. Oncotarget. 2017;8:45928-45942 pubmed publisher
flow cytometry; human; loading ...; fig 6a	Li J, Shayan G, Avery L, Jie H, Gildener Leapman N, Schmitt N, et al. Tumor-infiltrating Tim-3+ T cells proliferate avidly except when PD-1 is co-expressed: Evidence for intracellular cross talk. Oncoimmunology. 2016;5:e1200778 pubmed
flow cytometry; human; loading ...; fig 4	Heath J, Newhook N, Comeau E, Gallant M, Fudge N, Grant M. NKG2C(+)CD57(+) Natural Killer Cell Expansion Parallels Cytomegalovirus-Specific CD8(+) T Cell Evolution towards Senescence. J Immunol Res. 2016;2016:7470124 pubmed publisher
	Sade Feldman M, Yizhak K, Bjorgaard S, Ray J, de Boer C, Jenkins R, et al. Defining T Cell States Associated with Response to Checkpoint Immunotherapy in Melanoma. Cell. 2018;175:998-1013.e20 pubmed publisher
	Su S, Zhao J, Xing Y, Zhang X, Liu J, Ouyang Q, et al. Immune Checkpoint Inhibition Overcomes ADCP-Induced Immunosuppression by Macrophages. Cell. 2018;175:442-457.e23 pubmed publisher
	Tang Y, Sheng S, Lu C, Zhang Y, Zou J, Lei Y, et al. Effects of Glycogen Synthase Kinase-3β Inhibitor TWS119 on Proliferation and Cytokine Production of TILs From Human Lung Cancer. J Immunother. 2018;41:319-328 pubmed publisher
	Carisey A, Mace E, Saeed M, Davis D, Orange J. Nanoscale Dynamism of Actin Enables Secretory Function in Cytolytic Cells. Curr Biol. 2018;28:489-502.e9 pubmed publisher
	Klein Geltink R, O Sullivan D, Corrado M, Bremser A, Buck M, Buescher J, et al. Mitochondrial Priming by CD28. Cell. 2017;171:385-397.e11 pubmed publisher
	Candia E, Reyes P, Covian C, Rodriguez F, Wainstein N, Morales J, et al. Single and combined effect of retinoic acid and rapamycin modulate the generation, activity and homing potential of induced human regulatory T cells. PLoS ONE. 2017;12:e0182009 pubmed publisher
	Fernández L, Métais J, Escudero A, Vela M, Valentín J, Vallcorba I, et al. Memory T Cells Expressing an NKG2D-CAR Efficiently Target Osteosarcoma Cells. Clin Cancer Res. 2017;23:5824-5835 pubmed publisher
	Blas Rus N, Bustos MorÃ¡n E, SÃ¡nchez Madrid F, MartÃn CÃ³freces N. Analysis of Microtubules and Microtubule-Organizing Center at the Immune Synapse. Methods Mol Biol. 2017;1584:31-49 pubmed publisher
	Jiao S, Xia W, Yamaguchi H, Wei Y, Chen M, Hsu J, et al. PARP Inhibitor Upregulates PD-L1 Expression and Enhances Cancer-Associated Immunosuppression. Clin Cancer Res. 2017;23:3711-3720 pubmed publisher
	Lund P, Elias J, Davis M. Global Analysis of O-GlcNAc Glycoproteins in Activated Human T Cells. J Immunol. 2016;197:3086-3098 pubmed
	Jiang J, Chen X, An H, Yang B, Zhang F, Cheng X. Enhanced immune response of MAIT cells in tuberculous pleural effusions depends on cytokine signaling. Sci Rep. 2016;6:32320 pubmed publisher
	Schmidt A, Eriksson M, Shang M, Weyd H, Tegner J. Comparative Analysis of Protocols to Induce Human CD4+Foxp3+ Regulatory T Cells by Combinations of IL-2, TGF-beta, Retinoic Acid, Rapamycin and Butyrate. PLoS ONE. 2016;11:e0148474 pubmed publisher
	Vivar O, Masi G, Carpier J, Magalhaes J, Galgano D, Pazour G, et al. IFT20 controls LAT recruitment to the immune synapse and T-cell activation in vivo. Proc Natl Acad Sci U S A. 2016;113:386-91 pubmed publisher
	Sriram U, Cenna J, Haldar B, Fernandes N, Razmpour R, Fan S, et al. Methamphetamine induces trace amine-associated receptor 1 (TAAR1) expression in human T lymphocytes: role in immunomodulation. J Leukoc Biol. 2016;99:213-23 pubmed publisher
	Bai A, Moss A, Rothweiler S, Serena Longhi M, Wu Y, Junger W, et al. NADH oxidase-dependent CD39 expression by CD8(+) T cells modulates interferon gamma responses via generation of adenosine. Nat Commun. 2015;6:8819 pubmed publisher
	Clayton K, Haaland M, Douglas Vail M, Mujib S, Chew G, Ndhlovu L, et al. T cell Ig and mucin domain-containing protein 3 is recruited to the immune synapse, disrupts stable synapse formation, and associates with receptor phosphatases. J Immunol. 2014;192:782-91 pubmed publisher
	East J, Sun W, Webb T. Artificial antigen presenting cell (aAPC) mediated activation and expansion of natural killer T cells. J Vis Exp. 2012;: pubmed publisher
	Apolloni A, Lin M, Sivakumaran H, Li D, Kershaw M, Harrich D. A mutant Tat protein provides strong protection from HIV-1 infection in human CD4+ T cells. Hum Gene Ther. 2013;24:270-82 pubmed publisher
	Mujib S, Jones R, Lo C, Aidarus N, Clayton K, Sakhdari A, et al. Antigen-independent induction of Tim-3 expression on human T cells by the common ?-chain cytokines IL-2, IL-7, IL-15, and IL-21 is associated with proliferation and is dependent on the phosphoinositide 3-kinase pathway. J Immunol. 2012;188:3745-56 pubmed publisher
	Newell E, Sigal N, Bendall S, Nolan G, Davis M. Cytometry by time-of-flight shows combinatorial cytokine expression and virus-specific cell niches within a continuum of CD8+ T cell phenotypes. Immunity. 2012;36:142-52 pubmed publisher

product information

Antigen :

CD28

Apps. Abbrev. :

Flow Cytometry, Costim, IP, IHC

Cat # :

302914

Clone :

CD28.2

Item :

LEAF™ Purified anti-human CD28

Isotype :

Mouse IgG1, κ

Other Names :

T44, Tp44

Size :

500 μg

Price (USD) :

145 USD

Reactivity :

Human, Baboon, Capuchin Monkey, Chimpanzee, Cynomolgus, Pigtailed Macaque, Rhesus, Sooty Mangabey, Squirrel Monkey

Clonality :

Monoclonal

Host :

Mouse

Conjugate/Tag/Label :

LEAF

Application Notes :

Additional reported applications (for the relevant formats) include: immunoprecipitation, immunohistochemical staining of acetone-fixed frozen tissue sections 4 , and in vitro T cell costimulation 5-8 . This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The CD28.2 antibody co-stimulates T cell proliferation and cytokine production in the presence of suboptimal amounts of anti-CD3 antibody. The LEAF purified antibody (Endotoxin 0.1 EU/ug, Azide-Free, 0.2 um filtered) is recommended for functional assays (Cat. No. 302914). For highly sensitive assays, we recommend Ultra-LEAF purified antibody (Cat. No. 302934) with a lower endotoxin limit than standard LEAF purified antibodies (Endotoxin 0.01 EU/ug).