product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Purified anti-human CD27
catalog :
302802
quantity :
100 μg
price :
44 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
O323
reactivity :
African green monkey, human, rhesus macaque
application :
flow cytometry, blocking or activating experiments, mass cytometry
more info or order :
citations: 54
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; fig 3g
Dalla Pietà A, Cappuzzello E, Palmerini P, Ventura A, Visentin A, Astori G, et al. Innovative therapeutic strategy for B-cell malignancies that combines obinutuzumab and cytokine-induced killer cells. J Immunother Cancer. 2021;9: pubmed publisher
  • flow cytometry; human; 1:200; loading ...
Turner J, Kim W, Kalaidina E, Goss C, Rauseo A, Schmitz A, et al. SARS-CoV-2 infection induces long-lived bone marrow plasma cells in humans. Nature. 2021;595:421-425 pubmed publisher
  • mass cytometry; human; loading ...; fig 4e
  • flow cytometry; human; loading ...; fig 2a
Ingelfinger F, Krishnarajah S, Kramer M, Utz S, Galli E, Lutz M, et al. Single-cell profiling of myasthenia gravis identifies a pathogenic T cell signature. Acta Neuropathol. 2021;141:901-915 pubmed publisher
  • flow cytometry; human; 1:50; loading ...
Zurli V, Montecchi T, Heilig R, Poschke I, Volkmar M, Wimmer G, et al. Phosphoproteomics of CD2 signaling reveals AMPK-dependent regulation of lytic granule polarization in cytotoxic T cells. Sci Signal. 2020;13: pubmed publisher
  • flow cytometry; human; loading ...; fig s1
Callender L, Carroll E, Bober E, Akbar A, Solito E, Henson S. Mitochondrial mass governs the extent of human T cell senescence. Aging Cell. 2020;19:e13067 pubmed publisher
  • flow cytometry; human; loading ...; fig 1a
Staniek J, Lorenzetti R, Heller B, Janowska I, Schneider P, Unger S, et al. TRAIL-R1 and TRAIL-R2 Mediate TRAIL-Dependent Apoptosis in Activated Primary Human B Lymphocytes. Front Immunol. 2019;10:951 pubmed publisher
  • flow cytometry; human; loading ...; fig 1a
Terahara K, Iwabuchi R, Hosokawa M, Nishikawa Y, Takeyama H, Takahashi Y, et al. A CCR5+ memory subset within HIV-1-infected primary resting CD4+ T cells is permissive for replication-competent, latently infected viruses in vitro. BMC Res Notes. 2019;12:242 pubmed publisher
  • flow cytometry; human; loading ...; fig 3a
Ye Y, Liu M, Tang L, Du F, Liu Y, Hao P, et al. Iguratimod represses B cell terminal differentiation linked with the inhibition of PKC/EGR1 axis. Arthritis Res Ther. 2019;21:92 pubmed publisher
  • flow cytometry; human; loading ...; fig 1b
Remmerswaal E, Hombrink P, Nota B, Pircher H, ten Berge I, van Lier R, et al. Expression of IL-7Rα and KLRG1 defines functionally distinct CD8+ T-cell populations in humans. Eur J Immunol. 2019;49:694-708 pubmed publisher
  • flow cytometry; human; loading ...; fig 3a
Montel Hagen A, Seet C, Li S, Chick B, Zhu Y, Chang P, et al. Organoid-Induced Differentiation of Conventional T Cells from Human Pluripotent Stem Cells. Cell Stem Cell. 2019;24:376-389.e8 pubmed publisher
  • flow cytometry; human; loading ...; fig s4b
Yeo L, Woodwyk A, Sood S, Lorenc A, Eichmann M, Pujol Autonell I, et al. Autoreactive T effector memory differentiation mirrors β cell function in type 1 diabetes. J Clin Invest. 2018;128:3460-3474 pubmed publisher
  • flow cytometry; human; loading ...; fig 1d
Amodio D, Cotugno N, Macchiarulo G, Rocca S, Dimopoulos Y, Castrucci M, et al. Quantitative Multiplexed Imaging Analysis Reveals a Strong Association between Immunogen-Specific B Cell Responses and Tonsillar Germinal Center Immune Dynamics in Children after Influenza Vaccination. J Immunol. 2018;200:538-550 pubmed publisher
  • mass cytometry; human; loading ...; fig 2a
Chew V, Lai L, Pan L, Lim C, Li J, Ong R, et al. Delineation of an immunosuppressive gradient in hepatocellular carcinoma using high-dimensional proteomic and transcriptomic analyses. Proc Natl Acad Sci U S A. 2017;114:E5900-E5909 pubmed publisher
  • flow cytometry; human; loading ...; fig 1d
Domae E, Hirai Y, Ikeo T, Goda S, Shimizu Y. Cytokine-mediated activation of human ex vivo-expanded V?9V?2 T cells. Oncotarget. 2017;8:45928-45942 pubmed publisher
  • flow cytometry; human; fig 1b
Arumugakani G, Stephenson S, Newton D, Rawstron A, Emery P, Doody G, et al. Early Emergence of CD19-Negative Human Antibody-Secreting Cells at the Plasmablast to Plasma Cell Transition. J Immunol. 2017;198:4618-4628 pubmed publisher
  • flow cytometry; human; loading ...; fig 2b
Wentink M, Dalm V, Lankester A, van Schouwenburg P, Schölvinck L, Kalina T, et al. Genetic defects in PI3K? affect B-cell differentiation and maturation leading to hypogammaglobulineamia and recurrent infections. Clin Immunol. 2017;176:77-86 pubmed publisher
  • flow cytometry; human; 1:100; loading ...; fig s2
Raposo R, de Mulder Rougvie M, Paquin Proulx D, Brailey P, Cabido V, Zdinak P, et al. IFITM1 targets HIV-1 latently infected cells for antibody-dependent cytolysis. JCI Insight. 2017;2:e85811 pubmed publisher
  • flow cytometry; human; loading ...; fig 1b
Tauriainen J, Scharf L, Frederiksen J, Naji A, Ljunggren H, Sonnerborg A, et al. Perturbed CD8+ T cell TIGIT/CD226/PVR axis despite early initiation of antiretroviral treatment in HIV infected individuals. Sci Rep. 2017;7:40354 pubmed publisher
  • flow cytometry; rhesus macaque; loading ...; fig 3a
Stanfield B, Pahar B, Chouljenko V, Veazey R, Kousoulas K. Vaccination of rhesus macaques with the live-attenuated HSV-1 vaccine VC2 stimulates the proliferation of mucosal T cells and germinal center responses resulting in sustained production of highly neutralizing antibodies. Vaccine. 2017;35:536-543 pubmed publisher
  • blocking or activating experiments; human; 10 ug/ml; loading ...; fig 8a
Izawa K, Martin E, Soudais C, Bruneau J, Boutboul D, Rodriguez R, et al. Inherited CD70 deficiency in humans reveals a critical role for the CD70-CD27 pathway in immunity to Epstein-Barr virus infection. J Exp Med. 2017;214:73-89 pubmed publisher
  • flow cytometry; human; loading ...; fig 1e
Ju X, Silveira P, Hsu W, Elgundi Z, Alingcastre R, Verma N, et al. The Analysis of CD83 Expression on Human Immune Cells Identifies a Unique CD83+-Activated T Cell Population. J Immunol. 2016;197:4613-4625 pubmed
  • flow cytometry; human; loading ...; fig 2a
Massaad M, Zhou J, Tsuchimoto D, Chou J, Jabara H, Janssen E, et al. Deficiency of base excision repair enzyme NEIL3 drives increased predisposition to autoimmunity. J Clin Invest. 2016;126:4219-4236 pubmed publisher
  • flow cytometry; human; loading ...; fig 3h
Jiang J, Chen X, An H, Yang B, Zhang F, Cheng X. Enhanced immune response of MAIT cells in tuberculous pleural effusions depends on cytokine signaling. Sci Rep. 2016;6:32320 pubmed publisher
  • flow cytometry; human; loading ...; fig 2b
Kagoya Y, Nakatsugawa M, Yamashita Y, Ochi T, Guo T, Anczurowski M, et al. BET bromodomain inhibition enhances T cell persistence and function in adoptive immunotherapy models. J Clin Invest. 2016;126:3479-94 pubmed publisher
  • flow cytometry; African green monkey; loading ...; fig 2b
Neumann B, Shi T, Gan L, Klippert A, Daskalaki M, Stolte Leeb N, et al. Comprehensive panel of cross-reacting monoclonal antibodies for analysis of different immune cells and their distribution in the common marmoset (Callithrix jacchus). J Med Primatol. 2016;45:139-46 pubmed publisher
  • flow cytometry; human; loading ...; fig 3
Zwang N, Zhang R, Germana S, Fan M, Hastings W, Cao A, et al. Selective Sparing of Human Tregs by Pharmacologic Inhibitors of the Phosphatidylinositol 3-Kinase and MEK Pathways. Am J Transplant. 2016;16:2624-38 pubmed publisher
  • flow cytometry; human; loading ...; fig 1a
Roan F, Stoklasek T, Whalen E, Molitor J, Bluestone J, Buckner J, et al. CD4+ Group 1 Innate Lymphoid Cells (ILC) Form a Functionally Distinct ILC Subset That Is Increased in Systemic Sclerosis. J Immunol. 2016;196:2051-2062 pubmed publisher
  • flow cytometry; rhesus macaque; loading ...; fig 5c
Javed A, Leuchte N, Neumann B, Sopper S, Sauermann U. Noncytolytic CD8+ Cell Mediated Antiviral Response Represents a Strong Element in the Immune Response of Simian Immunodeficiency Virus-Infected Long-Term Non-Progressing Rhesus Macaques. PLoS ONE. 2015;10:e0142086 pubmed publisher
  • flow cytometry; human; fig 1
Frederiksen J, Buggert M, Noyan K, Nowak P, Sönnerborg A, Lund O, et al. Multidimensional Clusters of CD4+ T Cell Dysfunction Are Primarily Associated with the CD4/CD8 Ratio in Chronic HIV Infection. PLoS ONE. 2015;10:e0137635 pubmed publisher
  • flow cytometry; human; fig 4
Schnorfeil F, Lichtenegger F, Emmerig K, Schlueter M, Neitz J, Draenert R, et al. T cells are functionally not impaired in AML: increased PD-1 expression is only seen at time of relapse and correlates with a shift towards the memory T cell compartment. J Hematol Oncol. 2015;8:93 pubmed publisher
  • flow cytometry; human
Pombo C, Wherry E, Gostick E, Price D, Betts M. Elevated Expression of CD160 and 2B4 Defines a Cytolytic HIV-Specific CD8+ T-Cell Population in Elite Controllers. J Infect Dis. 2015;212:1376-86 pubmed publisher
  • flow cytometry; human; fig 1
Inui M, Hirota S, Hirano K, Fujii H, Sugahara Tobinai A, Ishii T, et al. Human CD43+ B cells are closely related not only to memory B cells phenotypically but also to plasmablasts developmentally in healthy individuals. Int Immunol. 2015;27:345-55 pubmed publisher
  • flow cytometry; human; fig 1
Boyle M, Jagannathan P, Bowen K, McIntyre T, Vance H, Farrington L, et al. Effector Phenotype of Plasmodium falciparum-Specific CD4+ T Cells Is Influenced by Both Age and Transmission Intensity in Naturally Exposed Populations. J Infect Dis. 2015;212:416-25 pubmed publisher
  • flow cytometry; human; fig 6
Lewis M, Vyse S, Shields A, Boeltz S, Gordon P, Spector T, et al. UBE2L3 polymorphism amplifies NF-κB activation and promotes plasma cell development, linking linear ubiquitination to multiple autoimmune diseases. Am J Hum Genet. 2015;96:221-34 pubmed publisher
  • flow cytometry; human; fig 2
Rissiek A, Baumann I, Cuapio A, Mautner A, Kolster M, Arck P, et al. The expression of CD39 on regulatory T cells is genetically driven and further upregulated at sites of inflammation. J Autoimmun. 2015;58:12-20 pubmed publisher
  • flow cytometry; human; loading ...; fig 1
Chang S, Kohrt H, Maecker H. Monitoring the immune competence of cancer patients to predict outcome. Cancer Immunol Immunother. 2014;63:713-9 pubmed publisher
Schwabenland M, Salié H, Tanevski J, Killmer S, Lago M, Schlaak A, et al. Deep spatial profiling of human COVID-19 brains reveals neuroinflammation with distinct microanatomical microglia-T-cell interactions. Immunity. 2021;54:1594-1610.e11 pubmed publisher
Roussel M, Ferrant J, Reizine F, Le Gallou S, Dulong J, Carl S, et al. Comparative immune profiling of acute respiratory distress syndrome patients with or without SARS-CoV-2 infection. Cell Rep Med. 2021;2:100291 pubmed publisher
Kaufmann M, Evans H, Schaupp A, Engler J, Kaur G, Willing A, et al. Identifying CNS-colonizing T cells as potential therapeutic targets to prevent progression of multiple sclerosis. Med (N Y). 2021;2:296-312.e8 pubmed publisher
Lakshmikanth T, Muhammad S, Olin A, Chen Y, Mikes J, Fagerberg L, et al. Human Immune System Variation during 1 Year. Cell Rep. 2020;32:107923 pubmed publisher
Eccles J, Turner R, Kirk N, Muehling L, Borish L, Steinke J, et al. T-bet+ Memory B Cells Link to Local Cross-Reactive IgG upon Human Rhinovirus Infection. Cell Rep. 2020;30:351-366.e7 pubmed publisher
Upasani V, Vo H, Ung S, Heng S, Laurent D, Choeung R, et al. Impaired Antibody-Independent Immune Response of B Cells in Patients With Acute Dengue Infection. Front Immunol. 2019;10:2500 pubmed publisher
Martin J, Chang C, Boschetti G, Ungaro R, Giri M, Grout J, et al. Single-Cell Analysis of Crohn's Disease Lesions Identifies a Pathogenic Cellular Module Associated with Resistance to Anti-TNF Therapy. Cell. 2019;178:1493-1508.e20 pubmed publisher
Kong R, Duan H, Sheng Z, Xu K, Acharya P, Chen X, et al. Antibody Lineages with Vaccine-Induced Antigen-Binding Hotspots Develop Broad HIV Neutralization. Cell. 2019;178:567-584.e19 pubmed publisher
Zimmerman M, Quicke K, O Neal J, Arora N, Machiah D, Priyamvada L, et al. Cross-Reactive Dengue Virus Antibodies Augment Zika Virus Infection of Human Placental Macrophages. Cell Host Microbe. 2018;24:731-742.e6 pubmed publisher
Sung J, Patel S, Clohosey M, Roesch L, Tripic T, Kuruc J, et al. HIV-Specific, Ex Vivo Expanded T Cell Therapy: Feasibility, Safety, and Efficacy in ART-Suppressed HIV-Infected Individuals. Mol Ther. 2018;26:2496-2506 pubmed publisher
Cole C, Byrne A, Beaudin A, Forsberg E, Vollmers C. Tn5Prime, a Tn5 based 5' capture method for single cell RNA-seq. Nucleic Acids Res. 2018;46:e62 pubmed publisher
Majri S, Fritz J, Villarino A, Zheng L, Kanellopoulou C, Chaigne Delalande B, et al. STAT5B: A Differential Regulator of the Life and Death of CD4+ Effector Memory T Cells. J Immunol. 2018;200:110-118 pubmed publisher
Renand A, Shamji M, Harris K, Qin T, Wambre E, Scadding G, et al. Synchronous immune alterations mirror clinical response during allergen immunotherapy. J Allergy Clin Immunol. 2018;141:1750-1760.e1 pubmed publisher
Bruno T, Ebner P, Moore B, Squalls O, Waugh K, Eruslanov E, et al. Antigen-Presenting Intratumoral B Cells Affect CD4+ TIL Phenotypes in Non-Small Cell Lung Cancer Patients. Cancer Immunol Res. 2017;5:898-907 pubmed publisher
Magnani D, Silveira C, Rosen B, Ricciardi M, Pedreño Lopez N, Gutman M, et al. A human inferred germline antibody binds to an immunodominant epitope and neutralizes Zika virus. PLoS Negl Trop Dis. 2017;11:e0005655 pubmed publisher
Lavin Y, Kobayashi S, Leader A, Amir E, Elefant N, Bigenwald C, et al. Innate Immune Landscape in Early Lung Adenocarcinoma by Paired Single-Cell Analyses. Cell. 2017;169:750-765.e17 pubmed publisher
Singh A, Novakova L, Axelsson M, Malmeström C, Zetterberg H, Lycke J, et al. High Interferon-γ Uniquely in Vδ1 T Cells Correlates with Markers of Inflammation and Axonal Damage in Early Multiple Sclerosis. Front Immunol. 2017;8:260 pubmed publisher
Crome S, Nguyen L, Lopez Verges S, Yang S, Martin B, Yam J, et al. A distinct innate lymphoid cell population regulates tumor-associated T cells. Nat Med. 2017;23:368-375 pubmed publisher
product information
Antigen :
CD27
Apps. Abbrev. :
FC, CyTOF®
Cat # :
302802
Clone :
O323
Item :
Purified anti-human CD27
Isotype :
Mouse IgG1, κ
Other Names :
S152, T14, TNFRSF7
Size :
100 μg
Price (USD) :
44 USD
Reactivity :
Human, Cynomolgus, Rhesus
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
PURE
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA