product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Pacific Blue™ anti-human CD20
catalog :
302319
quantity :
25 μg
price :
124 USD
clonality :
monoclonal
host :
mouse
conjugate :
Pacific Blue
clone name :
2H7
reactivity :
human, rhesus macaque
application :
flow cytometry
more info or order :
citations: 22
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; 1:400; loading ...; fig 3
Turner J, Kim W, Kalaidina E, Goss C, Rauseo A, Schmitz A, et al. SARS-CoV-2 infection induces long-lived bone marrow plasma cells in humans. Nature. 2021;595:421-425 pubmed publisher
  • flow cytometry; human; loading ...; fig s1c
Wentink M, Dalm V, Lankester A, van Schouwenburg P, Schölvinck L, Kalina T, et al. Genetic defects in PI3K? affect B-cell differentiation and maturation leading to hypogammaglobulineamia and recurrent infections. Clin Immunol. 2017;176:77-86 pubmed publisher
Turner J, O Halloran J, Kalaidina E, Kim W, Schmitz A, Zhou J, et al. SARS-CoV-2 mRNA vaccines induce persistent human germinal centre responses. Nature. 2021;596:109-113 pubmed publisher
Leach S, Gibbings S, Tewari A, Atif S, Vestal B, Danhorn T, et al. Human and Mouse Transcriptome Profiling Identifies Cross-Species Homology in Pulmonary and Lymph Node Mononuclear Phagocytes. Cell Rep. 2020;33:108337 pubmed publisher
Leylek R, Alcántara Hernández M, Lanzar Z, Lüdtke A, Perez O, Reizis B, et al. Integrated Cross-Species Analysis Identifies a Conserved Transitional Dendritic Cell Population. Cell Rep. 2019;29:3736-3750.e8 pubmed publisher
Hagan T, Cortese M, Rouphael N, Boudreau C, Linde C, Maddur M, et al. Antibiotics-Driven Gut Microbiome Perturbation Alters Immunity to Vaccines in Humans. Cell. 2019;178:1313-1328.e13 pubmed publisher
Yang C, Siebert J, Burns R, Gerbec Z, Bonacci B, Rymaszewski A, et al. Heterogeneity of human bone marrow and blood natural killer cells defined by single-cell transcriptome. Nat Commun. 2019;10:3931 pubmed publisher
van Dongen J, van der Burg M, Kalina T, Perez Andres M, Mejstrikova E, Vlkova M, et al. EuroFlow-Based Flowcytometric Diagnostic Screening and Classification of Primary Immunodeficiencies of the Lymphoid System. Front Immunol. 2019;10:1271 pubmed publisher
Matzen S, Raaschou Jensen K, Kallenbach K. Implementation of the Ogata flow cytometric scoring system in routine diagnostics of myelodysplastic syndrome. Health Sci Rep. 2018;1:e90 pubmed publisher
Suan D, Kräutler N, Mååg J, Butt D, Bourne K, Hermes J, et al. CCR6 Defines Memory B Cell Precursors in Mouse and Human Germinal Centers, Revealing Light-Zone Location and Predominant Low Antigen Affinity. Immunity. 2017;47:1142-1153.e4 pubmed publisher
Alcántara Hernández M, Leylek R, Wagar L, Engleman E, Keler T, Marinkovich M, et al. High-Dimensional Phenotypic Mapping of Human Dendritic Cells Reveals Interindividual Variation and Tissue Specialization. Immunity. 2017;47:1037-1050.e6 pubmed publisher
van der Velden V, Flores Montero J, Perez Andres M, Martin Ayuso M, Crespo O, Blanco E, et al. Optimization and testing of dried antibody tube: The EuroFlow LST and PIDOT tubes as examples. J Immunol Methods. 2017;: pubmed publisher
Barman S, Kayama H, Okuzaki D, Ogino T, Osawa H, Matsuno H, et al. Identification of a human intestinal myeloid cell subset that regulates gut homeostasis. Int Immunol. 2016;28:533-545 pubmed
Ma C, Wong N, Rao G, Nguyen A, Avery D, Payne K, et al. Unique and shared signaling pathways cooperate to regulate the differentiation of human CD4+ T cells into distinct effector subsets. J Exp Med. 2016;213:1589-608 pubmed publisher
Rother M, Schreurs M, Kroek R, Bartol S, van Dongen J, van Zelm M. The Human Thymus Is Enriched for Autoreactive B Cells. J Immunol. 2016;197:441-8 pubmed publisher
Del Prete G, Oswald K, Lara A, Shoemaker R, Smedley J, Macallister R, et al. Elevated Plasma Viral Loads in Romidepsin-Treated Simian Immunodeficiency Virus-Infected Rhesus Macaques on Suppressive Combination Antiretroviral Therapy. Antimicrob Agents Chemother. 2015;60:1560-72 pubmed publisher
Ma C, Wong N, Rao G, Avery D, Torpy J, Hambridge T, et al. Monogenic mutations differentially affect the quantity and quality of T follicular helper cells in patients with human primary immunodeficiencies. J Allergy Clin Immunol. 2015;136:993-1006.e1 pubmed publisher
Li H, Evans T, Gillis J, Connole M, Reeves R. Bone marrow-imprinted gut-homing of plasmacytoid dendritic cells (pDCs) in acute simian immunodeficiency virus infection results in massive accumulation of hyperfunctional CD4+ pDCs in the mucosae. J Infect Dis. 2015;211:1717-25 pubmed publisher
Del Prete G, Shoemaker R, Oswald K, Lara A, Trubey C, Fast R, et al. Effect of suberoylanilide hydroxamic acid (SAHA) administration on the residual virus pool in a model of combination antiretroviral therapy-mediated suppression in SIVmac239-infected indian rhesus macaques. Antimicrob Agents Chemother. 2014;58:6790-806 pubmed publisher
Rusmini M, Griseri P, Lantieri F, Matera I, Hudspeth K, Roberto A, et al. Induction of RET dependent and independent pro-inflammatory programs in human peripheral blood mononuclear cells from Hirschsprung patients. PLoS ONE. 2013;8:e59066 pubmed publisher
Moore A, Bixler S, Lewis M, Verthelyi D, Mattapallil J. Mucosal and peripheral Lin- HLA-DR+ CD11c/123- CD13+ CD14- mononuclear cells are preferentially infected during acute simian immunodeficiency virus infection. J Virol. 2012;86:1069-78 pubmed publisher
Davis Z, Ward J, Barker E. Preparation and use of HIV-1 infected primary CD4+ T-cells as target cells in natural killer cell cytotoxic assays. J Vis Exp. 2011;: pubmed publisher
product information
Antigen :
CD20
Apps. Abbrev. :
FC
Cat # :
302319
Clone :
2H7
Item :
Pacific Blue™ anti-human CD20
Isotype :
Mouse IgG2b, κ
Other Names :
B1, Bp35
Size :
25 μg
Price (USD) :
124 USD
Reactivity :
Human, Cynomolgus, Rhesus
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
PB
Immunogen :
Human tonsillar B cells
Application Notes :
The epitope recognized by clone 2H7 has been mapped to the sequence YNCEPANPSEKNSPST which lies in the large extracellular loop of human CD20. Additional reported applications (for the relevant formats) include: immunoprecipitation^4 and immunohistochemical staining of acetone-fixed frozen sections^5.;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA