product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Purified anti-human CD20
catalog :
302302
quantity :
100 μg
price :
78 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
2H7
reactivity :
African green monkey, human, mouse, rhesus macaque
application :
immunoprecipitation, flow cytometry, immunohistochemistry - frozen section, mass cytometry
more info or order :
citations: 51
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; loading ...; fig s2a
Reijm S, Kissel T, Stoeken Rijsbergen G, Slot L, Wortel C, van Dooren H, et al. Cross-reactivity of IgM anti-modified protein antibodies in rheumatoid arthritis despite limited mutational load. Arthritis Res Ther. 2021;23:230 pubmed publisher
  • flow cytometry; human; loading ...; fig 1a
Yue X, Petersen F, Shu Y, Kasper B, Magatsin J, Ahmadi M, et al. Transfer of PBMC From SSc Patients Induces Autoantibodies and Systemic Inflammation in Rag2-/-/IL2rg-/- Mice. Front Immunol. 2021;12:677970 pubmed publisher
  • flow cytometry; human; loading ...
Bohannon C, Ende Z, Cao W, Mboko W, Ranjan P, Kumar A, et al. Influenza Virus Infects and Depletes Activated Adaptive Immune Responders. Adv Sci (Weinh). 2021;8:e2100693 pubmed publisher
  • flow cytometry; human; 1:400; loading ...; fig 3
Turner J, Kim W, Kalaidina E, Goss C, Rauseo A, Schmitz A, et al. SARS-CoV-2 infection induces long-lived bone marrow plasma cells in humans. Nature. 2021;595:421-425 pubmed publisher
  • mass cytometry; human; loading ...; fig 4e
Ingelfinger F, Krishnarajah S, Kramer M, Utz S, Galli E, Lutz M, et al. Single-cell profiling of myasthenia gravis identifies a pathogenic T cell signature. Acta Neuropathol. 2021;141:901-915 pubmed publisher
  • flow cytometry; human; 1:200; loading ...; fig s1h
Biswas S, Mandal G, Payne K, Anadon C, Gatenbee C, Chaurio R, et al. IgA transcytosis and antigen recognition govern ovarian cancer immunity. Nature. 2021;591:464-470 pubmed publisher
  • flow cytometry; mouse; 1:500; loading ...; fig s1b
Gang E, Kim H, Hsieh Y, Ruan Y, Ogana H, Lee S, et al. Integrin α6 mediates the drug resistance of acute lymphoblastic B-cell leukemia. Blood. 2020;136:210-223 pubmed publisher
  • flow cytometry; rhesus macaque; loading ...; fig 2d
Chea L, Wyatt L, Gangadhara S, Moss B, Amara R. Novel Modified Vaccinia Virus Ankara Vector Expressing Anti-apoptotic Gene B13R Delays Apoptosis and Enhances Humoral Responses. J Virol. 2019;93: pubmed publisher
  • flow cytometry; human; loading ...; fig s6d
Bradley T, Peppa D, Pedroza Pacheco I, Li D, Cain D, Henao R, et al. RAB11FIP5 Expression and Altered Natural Killer Cell Function Are Associated with Induction of HIV Broadly Neutralizing Antibody Responses. Cell. 2018;175:387-399.e17 pubmed publisher
  • flow cytometry; human; loading ...; fig 6b
Dam E, Maier A, Hocking A, Carlin J, Buckner J. Increased Binding of Specificity Protein 1 to the IL21R Promoter in B Cells Results in Enhanced B Cell Responses in Rheumatoid Arthritis. Front Immunol. 2018;9:1978 pubmed publisher
  • flow cytometry; human; loading ...; fig 1a
Olin A, Henckel E, Chen Y, Lakshmikanth T, Pou C, Mikes J, et al. Stereotypic Immune System Development in Newborn Children. Cell. 2018;174:1277-1292.e14 pubmed publisher
  • flow cytometry; human; loading ...; fig 1b
Moysi E, Pallikkuth S, de Armas L, Gonzalez L, Ambrozak D, George V, et al. Altered immune cell follicular dynamics in HIV infection following influenza vaccination. J Clin Invest. 2018;128:3171-3185 pubmed publisher
  • flow cytometry; human; loading ...; fig s2a
Wang L, Shi W, Chappell J, Joyce M, Zhang Y, Kanekiyo M, et al. Importance of Neutralizing Monoclonal Antibodies Targeting Multiple Antigenic Sites on the Middle East Respiratory Syndrome Coronavirus Spike Glycoprotein To Avoid Neutralization Escape. J Virol. 2018;92: pubmed publisher
  • flow cytometry; human; loading ...; fig 1b
Amodio D, Cotugno N, Macchiarulo G, Rocca S, Dimopoulos Y, Castrucci M, et al. Quantitative Multiplexed Imaging Analysis Reveals a Strong Association between Immunogen-Specific B Cell Responses and Tonsillar Germinal Center Immune Dynamics in Children after Influenza Vaccination. J Immunol. 2018;200:538-550 pubmed publisher
  • flow cytometry; human; fig 1a
Kyoizumi S, Kubo Y, Kajimura J, Yoshida K, Hayashi T, Nakachi K, et al. Fate Decision Between Group 3 Innate Lymphoid and Conventional NK Cell Lineages by Notch Signaling in Human Circulating Hematopoietic Progenitors. J Immunol. 2017;199:2777-2793 pubmed publisher
  • flow cytometry; human; loading ...; fig s1c
Wentink M, Dalm V, Lankester A, van Schouwenburg P, Schölvinck L, Kalina T, et al. Genetic defects in PI3K? affect B-cell differentiation and maturation leading to hypogammaglobulineamia and recurrent infections. Clin Immunol. 2017;176:77-86 pubmed publisher
  • flow cytometry; rhesus macaque; loading ...
Roberts E, Carnathan D, Li H, Shaw G, Silvestri G, Betts M. Collapse of Cytolytic Potential in SIV-Specific CD8+ T Cells Following Acute SIV Infection in Rhesus Macaques. PLoS Pathog. 2016;12:e1006135 pubmed publisher
  • flow cytometry; rhesus macaque; loading ...; fig 7a
Kasturi S, Kozlowski P, Nakaya H, Burger M, Russo P, Pham M, et al. Adjuvanting a Simian Immunodeficiency Virus Vaccine with Toll-Like Receptor Ligands Encapsulated in Nanoparticles Induces Persistent Antibody Responses and Enhanced Protection in TRIM5α Restrictive Macaques. J Virol. 2017;91: pubmed publisher
  • flow cytometry; human; fig s4a
Serr I, Fürst R, Ott V, Scherm M, Nikolaev A, Gökmen F, et al. miRNA92a targets KLF2 and the phosphatase PTEN signaling to promote human T follicular helper precursors in T1D islet autoimmunity. Proc Natl Acad Sci U S A. 2016;113:E6659-E6668 pubmed
  • flow cytometry; human; loading ...; fig 1a
Keefer M, Zheng B, Rosenberg A, Kobie J. Increased Steady-State Memory B Cell Subsets Among High-Risk Participants in an HIV Vaccine Trial. AIDS Res Hum Retroviruses. 2016;32:1143-1148 pubmed
  • flow cytometry; human; loading ...
Cerny D, Thi Le D, The T, Zuest R, Kg S, Velumani S, et al. Complete human CD1a deficiency on Langerhans cells due to a rare point mutation in the coding sequence. J Allergy Clin Immunol. 2016;138:1709-1712.e11 pubmed publisher
  • flow cytometry; human; loading ...; fig 1a
Piepenbrink M, Samuel M, Zheng B, Carter B, Fucile C, Bunce C, et al. Humoral Dysregulation Associated with Increased Systemic Inflammation among Injection Heroin Users. PLoS ONE. 2016;11:e0158641 pubmed publisher
  • flow cytometry; human; fig 6a
Wittmann A, Lamprinaki D, Bowles K, Katzenellenbogen E, Knirel Y, Whitfield C, et al. Dectin-2 Recognizes Mannosylated O-antigens of Human Opportunistic Pathogens and Augments Lipopolysaccharide Activation of Myeloid Cells. J Biol Chem. 2016;291:17629-38 pubmed publisher
  • flow cytometry; human; loading ...; tbl 1
Li H, Borrego F, Nagata S, Tolnay M. Fc Receptor-like 5 Expression Distinguishes Two Distinct Subsets of Human Circulating Tissue-like Memory B Cells. J Immunol. 2016;196:4064-74 pubmed publisher
  • flow cytometry; human; loading ...; tbl 1
Pojero F, Flores Montero J, Sanoja L, Pérez J, Puig N, Paiva B, et al. Utility of CD54, CD229, and CD319 for the identification of plasma cells in patients with clonal plasma cell diseases. Cytometry B Clin Cytom. 2016;90:91-100 pubmed publisher
  • flow cytometry; human; loading ...; fig 5a
Kerkman P, Fabre E, van der Voort E, Zaldumbide A, Rombouts Y, Rispens T, et al. Identification and characterisation of citrullinated antigen-specific B cells in peripheral blood of patients with rheumatoid arthritis. Ann Rheum Dis. 2016;75:1170-6 pubmed publisher
  • flow cytometry; human
Strick Marchand H, Dusséaux M, Darche S, Huntington N, Legrand N, Masse Ranson G, et al. A novel mouse model for stable engraftment of a human immune system and human hepatocytes. PLoS ONE. 2015;10:e0119820 pubmed publisher
  • flow cytometry; human; fig 1
Inui M, Hirota S, Hirano K, Fujii H, Sugahara Tobinai A, Ishii T, et al. Human CD43+ B cells are closely related not only to memory B cells phenotypically but also to plasmablasts developmentally in healthy individuals. Int Immunol. 2015;27:345-55 pubmed publisher
  • flow cytometry; African green monkey
Kwun J, Page E, Hong J, GIBBY A, Yoon J, Farris A, et al. Neutralizing BAFF/APRIL with atacicept prevents early DSA formation and AMR development in T cell depletion induced nonhuman primate AMR model. Am J Transplant. 2015;15:815-22 pubmed publisher
  • flow cytometry; rhesus macaque
Li H, Evans T, Gillis J, Connole M, Reeves R. Bone marrow-imprinted gut-homing of plasmacytoid dendritic cells (pDCs) in acute simian immunodeficiency virus infection results in massive accumulation of hyperfunctional CD4+ pDCs in the mucosae. J Infect Dis. 2015;211:1717-25 pubmed publisher
  • flow cytometry; rhesus macaque
Del Prete G, Shoemaker R, Oswald K, Lara A, Trubey C, Fast R, et al. Effect of suberoylanilide hydroxamic acid (SAHA) administration on the residual virus pool in a model of combination antiretroviral therapy-mediated suppression in SIVmac239-infected indian rhesus macaques. Antimicrob Agents Chemother. 2014;58:6790-806 pubmed publisher
  • flow cytometry; human
Kusner L, Ciesielski M, Marx A, Kaminski H, Fenstermaker R. Survivin as a potential mediator to support autoreactive cell survival in myasthenia gravis: a human and animal model study. PLoS ONE. 2014;9:e102231 pubmed publisher
Molina M, Hoffman E, Stokes J, Kummet N, Smith K, Baker F, et al. Regulatory Dendritic Cells Induced by Bendamustine Are Associated With Enhanced Flt3 Expression and Alloreactive T-Cell Death. Front Immunol. 2021;12:699128 pubmed publisher
Henrick B, Rodriguez L, Lakshmikanth T, Pou C, Henckel E, Arzoomand A, et al. Bifidobacteria-mediated immune system imprinting early in life. Cell. 2021;184:3884-3898.e11 pubmed publisher
Chevrier S, Zurbuchen Y, Cervia C, Adamo S, Raeber M, de Souza N, et al. A distinct innate immune signature marks progression from mild to severe COVID-19. Cell Rep Med. 2021;2:100166 pubmed publisher
Yang R, Mele F, Worley L, Langlais D, Rosain J, Benhsaien I, et al. Human T-bet Governs Innate and Innate-like Adaptive IFN-γ Immunity against Mycobacteria. Cell. 2020;183:1826-1847.e31 pubmed publisher
Gruber C, Calis J, Buta S, Evrony G, Martin J, Uhl S, et al. Complex Autoinflammatory Syndrome Unveils Fundamental Principles of JAK1 Kinase Transcriptional and Biochemical Function. Immunity. 2020;53:672-684.e11 pubmed publisher
Joas S, Sauermann U, Roshani B, Klippert A, Daskalaki M, Mätz Rensing K, et al. Nef-Mediated CD3-TCR Downmodulation Dampens Acute Inflammation and Promotes SIV Immune Evasion. Cell Rep. 2020;30:2261-2274.e7 pubmed publisher
Eccles J, Turner R, Kirk N, Muehling L, Borish L, Steinke J, et al. T-bet+ Memory B Cells Link to Local Cross-Reactive IgG upon Human Rhinovirus Infection. Cell Rep. 2020;30:351-366.e7 pubmed publisher
Li Z, Hu J, Qin Z, Tao Y, Lai Z, Wang Q, et al. High-dimensional single-cell proteomics analysis reveals the landscape of immune cells and stem-like cells in renal tumors. J Clin Lab Anal. 2020;34:e23155 pubmed publisher
Chng M, Lim M, Rouers A, Becht E, Lee B, Macary P, et al. Large-Scale HLA Tetramer Tracking of T Cells during Dengue Infection Reveals Broad Acute Activation and Differentiation into Two Memory Cell Fates. Immunity. 2019;51:1119-1135.e5 pubmed publisher
Kong R, Duan H, Sheng Z, Xu K, Acharya P, Chen X, et al. Antibody Lineages with Vaccine-Induced Antigen-Binding Hotspots Develop Broad HIV Neutralization. Cell. 2019;178:567-584.e19 pubmed publisher
Agrawal N, Streaţă I, Pei G, Weiner J, Kotze L, Bandermann S, et al. Human Monocytic Suppressive Cells Promote Replication of Mycobacterium tuberculosis and Alter Stability of in vitro Generated Granulomas. Front Immunol. 2018;9:2417 pubmed publisher
Zhong J, Scholz T, Yau A, Guérard S, Huffmeier U, Burkhardt H, et al. Mannan-induced Nos2 in macrophages enhances IL-17-driven psoriatic arthritis by innate lymphocytes. Sci Adv. 2018;4:eaas9864 pubmed publisher
Evrard M, Kwok I, Chong S, Teng K, Becht E, Chen J, et al. Developmental Analysis of Bone Marrow Neutrophils Reveals Populations Specialized in Expansion, Trafficking, and Effector Functions. Immunity. 2018;48:364-379.e8 pubmed publisher
Sander J, Schmidt S, Cirovic B, McGovern N, Papantonopoulou O, Hardt A, et al. Cellular Differentiation of Human Monocytes Is Regulated by Time-Dependent Interleukin-4 Signaling and the Transcriptional Regulator NCOR2. Immunity. 2017;47:1051-1066.e12 pubmed publisher
Gunawan M, Her Z, Liu M, Tan S, Chan X, Tan W, et al. A Novel Human Systemic Lupus Erythematosus Model in Humanised Mice. Sci Rep. 2017;7:16642 pubmed publisher
Bruno T, Ebner P, Moore B, Squalls O, Waugh K, Eruslanov E, et al. Antigen-Presenting Intratumoral B Cells Affect CD4+ TIL Phenotypes in Non-Small Cell Lung Cancer Patients. Cancer Immunol Res. 2017;5:898-907 pubmed publisher
Cottineau J, Kottemann M, Lach F, Kang Y, Vély F, Deenick E, et al. Inherited GINS1 deficiency underlies growth retardation along with neutropenia and NK cell deficiency. J Clin Invest. 2017;127:1991-2006 pubmed publisher
Tornack J, Reece S, Bauer W, Vogelzang A, Bandermann S, Zedler U, et al. Human and Mouse Hematopoietic Stem Cells Are a Depot for Dormant Mycobacterium tuberculosis. PLoS ONE. 2017;12:e0169119 pubmed publisher
Murayama M, Kakuta S, Inoue A, Umeda N, Yonezawa T, Maruhashi T, et al. CTRP6 is an endogenous complement regulator that can effectively treat induced arthritis. Nat Commun. 2015;6:8483 pubmed publisher
product information
Antigen :
CD20
Apps. Abbrev. :
FC, CyTOF®, IHC-F, IP
Cat # :
302302
Clone :
2H7
Item :
Purified anti-human CD20
Isotype :
Mouse IgG2b, κ
Other Names :
B1, Bp35
Size :
100 μg
Price (USD) :
78 USD
Reactivity :
Human, Cynomolgus, Rhesus
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
PURE
Immunogen :
Human tonsillar B cells
Application Notes :
The epitope recognized by clone 2H7 has been mapped to the sequence YNCEPANPSEKNSPST which lies in the large extracellular loop of human CD20. Additional reported applications (for the relevant formats) include: immunoprecipitation^4 and immunohistochemical staining of acetone-fixed frozen sections^5.;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA