product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Purified anti-human CD18
catalog :
302102
quantity :
100 μg
price :
112 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
TS1/18
reactivity :
human, mouse
application :
neutralization, flow cytometry, blocking or activating experiments
more info or order :
citations: 22
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; loading ...; fig s1
Matsumoto K, Kurasawa T, Yoshimoto K, Suzuki K, Takeuchi T. Identification of neutrophil β2-integrin LFA-1 as a potential mechanistic biomarker in ANCA-associated vasculitis via microarray and validation analyses. Arthritis Res Ther. 2021;23:136 pubmed publisher
  • flow cytometry; human; fig 1b
Buffone A, Anderson N, Hammer D. Migration against the direction of flow is LFA-1-dependent in human hematopoietic stem and progenitor cells. J Cell Sci. 2018;131: pubmed publisher
  • flow cytometry; human; fig 3a
Janesch P, Rouha H, Weber S, Malafa S, Gross K, Maierhofer B, et al. Selective sensitization of human neutrophils to LukGH mediated cytotoxicity by Staphylococcus aureus and IL-8. J Infect. 2017;74:473-483 pubmed publisher
  • blocking or activating experiments; human; loading ...; fig s6b
Park R, Wang T, Koundakjian D, Hultquist J, Lamothe Molina P, Monel B, et al. A genome-wide CRISPR screen identifies a restricted set of HIV host dependency factors. Nat Genet. 2017;49:193-203 pubmed publisher
  • blocking or activating experiments; human; loading ...; fig 5c
Yeap W, Wong K, Shimasaki N, Teo E, Quek J, Yong H, et al. CD16 is indispensable for antibody-dependent cellular cytotoxicity by human monocytes. Sci Rep. 2016;6:34310 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 8a
Guo H, Cranert S, Lu Y, Zhong M, Zhang S, Chen J, et al. Deletion of Slam locus in mice reveals inhibitory role of SLAM family in NK cell responses regulated by cytokines and LFA-1. J Exp Med. 2016;213:2187-207 pubmed publisher
  • flow cytometry; human; loading ...; fig 2a
Curtis B, Roman A, Sullivan M, Raven C, Larison J, Weitekamp L. Two cases of maternal alloimmunization against human neutrophil alloantigen-4b, one causing severe alloimmune neonatal neutropenia. Transfusion. 2016;56:101-6 pubmed publisher
  • flow cytometry; human; fig 5
Difranco K, Johnson Farley N, Bertino J, Elson D, Vega B, Belinka B, et al. LFA-1-targeting Leukotoxin (LtxA; Leukothera®) causes lymphoma tumor regression in a humanized mouse model and requires caspase-8 and Fas to kill malignant lymphocytes. Leuk Res. 2015;39:649-56 pubmed publisher
  • blocking or activating experiments; human
Marinho C, Azeredo E, Torrentes Carvalho A, Marins Dos Santos A, Kubelka C, de Souza L, et al. Down-regulation of complement receptors on the surface of host monocyte even as in vitro complement pathway blocking interferes in dengue infection. PLoS ONE. 2014;9:e102014 pubmed publisher
  • flow cytometry; human
Lozano Fernández T, Ballester Antxordoki L, Pérez Temprano N, Rojas E, Sanz D, Iglesias Gaspar M, et al. Potential impact of metal oxide nanoparticles on the immune system: The role of integrins, L-selectin and the chemokine receptor CXCR4. Nanomedicine. 2014;10:1301-10 pubmed publisher
Chong D, Rebeyrol C, José R, Williams A, Brown J, Scotton C, et al. ICAM-1 and ICAM-2 Are Differentially Expressed and Up-Regulated on Inflamed Pulmonary Epithelium, but Neither ICAM-2 nor LFA-1: ICAM-1 Are Required for Neutrophil Migration Into the Airways In Vivo. Front Immunol. 2021;12:691957 pubmed publisher
Myint P, Ito A, Appiah M, Obeng G, Darkwah S, Kawamoto E, et al. Irisin supports integrin-mediated cell adhesion of lymphocytes. Biochem Biophys Rep. 2021;26:100977 pubmed publisher
Kolev M, West E, Kunz N, Chauss D, Moseman E, Rahman J, et al. Diapedesis-Induced Integrin Signaling via LFA-1 Facilitates Tissue Immunity by Inducing Intrinsic Complement C3 Expression in Immune Cells. Immunity. 2020;52:513-527.e8 pubmed publisher
Ristow L, Tran V, Schwartz K, Pankratz L, Mehle A, Sauer J, et al. The Extracellular Domain of the β2 Integrin β Subunit (CD18) Is Sufficient for Escherichia coli Hemolysin and Aggregatibacter actinomycetemcomitans Leukotoxin Cytotoxic Activity. MBio. 2019;10: pubmed publisher
Lee C, Zhang H, Singh S, Koo L, Kabat J, Tsang H, et al. C/EBPδ drives interactions between human MAIT cells and endothelial cells that are important for extravasation. elife. 2018;7: pubmed publisher
Li N, Yang H, Wang M, Lü S, Zhang Y, Long M. Ligand-specific binding forces of LFA-1 and Mac-1 in neutrophil adhesion and crawling. Mol Biol Cell. 2018;29:408-418 pubmed publisher
Hosoki K, Nakamura A, Nagao M, Hiraguchi Y, Tanida H, Tokuda R, et al. Staphylococcus aureus directly activates eosinophils via platelet-activating factor receptor. J Leukoc Biol. 2012;92:333-41 pubmed publisher
Sommaggio R, Cohnen A, Watzl C, Costa C. Multiple receptors trigger human NK cell-mediated cytotoxicity against porcine chondrocytes. J Immunol. 2012;188:2075-83 pubmed publisher
Chakrabarty S, Snyder J, Shen J, Azmi H, Hu P, Chen Q, et al. Human CD14hi monocytes and myeloid dendritic cells provide a cell contact-dependent costimulatory signal for early CD40 ligand expression. Blood. 2011;117:1585-94 pubmed publisher
Tran D, Glass D, Uzel G, Darnell D, Spalding C, Holland S, et al. Analysis of adhesion molecules, target cells, and role of IL-2 in human FOXP3+ regulatory T cell suppressor function. J Immunol. 2009;182:2929-38 pubmed publisher
Kanda H, Newton R, Klein R, Morita Y, Gunn M, Rosen S. Autotaxin, an ectoenzyme that produces lysophosphatidic acid, promotes the entry of lymphocytes into secondary lymphoid organs. Nat Immunol. 2008;9:415-23 pubmed publisher
Nakayama H, Yoshizaki F, Prinetti A, Sonnino S, Mauri L, Takamori K, et al. Lyn-coupled LacCer-enriched lipid rafts are required for CD11b/CD18-mediated neutrophil phagocytosis of nonopsonized microorganisms. J Leukoc Biol. 2008;83:728-41 pubmed
product information
Antigen :
CD18
Apps. Abbrev. :
Flow Cytometry, Block
Cat # :
302102
Clone :
TS1/18
Item :
Purified anti-human CD18
Isotype :
Mouse IgG1, κ
Other Names :
Integrin β2 subunit, LFA -1 β subunit, β2 integrin, ITGB2
Size :
100 μg
Price (USD) :
112 USD
Reactivity :
Human
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
PURE
Application Notes :
Additional reported applications (for the relevant formats) include: inhibition of cell adhesion and migration 3,4 . The Ultra-LEAF purified antibody (Endotoxin 0.01 EU/ug, Azide-Free, 0.2 um filtered) (Cat. No. 302116) is recommended for functional assays.
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA