TotalSeq™-C0083 anti-human CD16 | BioLegend 302065 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

TotalSeq™-C0083 anti-human CD16

catalog :

302065

quantity :

10 μg

price :

369 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

3G8

reactivity :

human, rhesus macaque

application :

flow cytometry, blocking or activating experiments

more info or order :

BioLegend product webpage

citations: 8

Published Application/Species/Sample/Dilution	Reference
blocking or activating experiments; human; 5 ug/ml; loading ...; fig s4	Zhu Y, Xie J, Shi J. Rac1/ROCK-driven membrane dynamics promote natural killer cell cytotoxicity via granzyme-induced necroptosis. BMC Biol. 2021;19:140 pubmed publisher
flow cytometry; human; loading ...; fig 4b	Muhammad F, Wang D, Montieth A, Lee S, Preble J, Foster C, et al. PD-1+ melanocortin receptor dependent-Treg cells prevent autoimmune disease. Sci Rep. 2019;9:16941 pubmed publisher
	Xu C, Yang J, Kosters A, Babcock B, Qiu P, Ghosn E. Comprehensive multi-omics single-cell data integration reveals greater heterogeneity in the human immune system. iScience. 2022;25:105123 pubmed publisher
	Li S, Hickey A, Shangguan S, Ehrenberg P, Geretz A, Butler L, et al. HLA-B∗46 associates with rapid HIV disease progression in Asian cohorts and prominent differences in NK cell phenotype. Cell Host Microbe. 2022;30:1173-1185.e8 pubmed publisher
	Bachireddy P, Azizi E, Burdziak C, Nguyen V, Ennis C, Maurer K, et al. Mapping the evolution of T cell states during response and resistance to adoptive cellular therapy. Cell Rep. 2021;37:109992 pubmed publisher
	Shangguan S, Ehrenberg P, Geretz A, Yum L, Kundu G, May K, et al. Monocyte-derived transcriptome signature indicates antibody-dependent cellular phagocytosis as a potential mechanism of vaccine-induced protection against HIV-1. elife. 2021;10: pubmed publisher
	Wang E, Zhou H, Nadorp B, Cayanan G, Chen X, Yeaton A, et al. Surface antigen-guided CRISPR screens identify regulators of myeloid leukemia differentiation. Cell Stem Cell. 2021;: pubmed publisher
	Morad G, Daisy C, Otu H, Libermann T, Dillon S, Moses M. Cdc42-Dependent Transfer of mir301 from Breast Cancer-Derived Extracellular Vesicles Regulates the Matrix Modulating Ability of Astrocytes at the Blood-Brain Barrier. Int J Mol Sci. 2020;21: pubmed publisher

product information

Antigen :

CD16

Apps. Abbrev. :

Cat # :

302065

Clone :

3G8

Item :

TotalSeq™-C0083 anti-human CD16

Isotype :

Mouse IgG1, κ

Other Names :

FcγRIII, Fc gamma receptor, Fc gamma receptor 3

Size :

10 μg

Price (USD) :

369 USD

Reactivity :

Human, Cynomolgus, Rhesus

Clonality :

Monoclonal

Host :

Mouse

Conjugate/Tag/Label :

TOTALSEQ_C

Immunogen :

Human PMN cells

Application Notes :

The 3G8 antibody clone blocks neutrophil phagocytosis and stimulates NK cell proliferation. It has been reported that this clone interacts with the FcγRIIa and FcγRIIIb receptors causing neutrophil activation and aggregation^18. Due to this phenomenon staining in whole blood may cause a reduction in the number of granulocytes or alter their scatter profile.Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections^6, immunoprecipitation^3, stimulation of NK cell proliferation^4, blocking of phagocytosis^5, and blocking of immunoglobulin binding to FcγRIII^7,8. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 302049, 302050, 302057, 302058).;

more info or order :

BioLegend product webpage