product summary
Loading...
company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
PE anti-human CD16
catalog :
302056
quantity :
100 μg
price :
244 USD
clonality :
monoclonal
host :
mouse
conjugate :
PE
clone name :
3G8
reactivity :
human, rhesus macaque
application :
flow cytometry
more info or order :
citations: 26
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human
Mandl M, Schmitz S, Weber C, Hristov M. Characterization of the CD14++CD16+ monocyte population in human bone marrow. PLoS ONE. 2014;9:e112140 pubmed publisher
  • flow cytometry; human
Jiang B, Wu X, Li X, Yang X, Zhou Y, Yan H, et al. Expansion of NK cells by engineered K562 cells co-expressing 4-1BBL and mMICA, combined with soluble IL-21. Cell Immunol. 2014;290:10-20 pubmed publisher
  • flow cytometry; human
Bendall S, Simonds E, Qiu P, Amir E, Krutzik P, Finck R, et al. Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum. Science. 2011;332:687-96 pubmed publisher
Afolabi L, Bi J, Li X, Adeshakin A, Adeshakin F, Wu H, et al. Synergistic Tumor Cytolysis by NK Cells in Combination With a Pan-HDAC Inhibitor, Panobinostat. Front Immunol. 2021;12:701671 pubmed publisher
Zong D, Huang B, LI Y, Lu Y, Xiang N, Guo C, et al. Chromatin accessibility landscapes of immune cells in rheumatoid arthritis nominate monocytes in disease pathogenesis. BMC Biol. 2021;19:79 pubmed publisher
Teijeira A, Garasa S, Gato M, Alfaro C, Miguéliz I, Cirella A, et al. CXCR1 and CXCR2 Chemokine Receptor Agonists Produced by Tumors Induce Neutrophil Extracellular Traps that Interfere with Immune Cytotoxicity. Immunity. 2020;52:856-871.e8 pubmed publisher
Manukyan G, Martirosyan A, Slavik L, Margaryan S, Ulehlova J, Mikulkova Z, et al. Anti-domain 1 β2 glycoprotein antibodies increase expression of tissue factor on monocytes and activate NK Cells and CD8+ cells in vitro. Auto Immun Highlights. 2020;11:5 pubmed publisher
Ostendorf L, Mothes R, van Koppen S, Lindquist R, Bellmann Strobl J, Asseyer S, et al. Low-Density Granulocytes Are a Novel Immunopathological Feature in Both Multiple Sclerosis and Neuromyelitis Optica Spectrum Disorder. Front Immunol. 2019;10:2725 pubmed publisher
Urlaub D, Zhao S, Blank N, Bergner R, Claus M, Tretter T, et al. Activation of natural killer cells by rituximab in granulomatosis with polyangiitis. Arthritis Res Ther. 2019;21:277 pubmed publisher
Woolsey C, Menicucci A, Cross R, Luthra P, Agans K, Borisevich V, et al. A VP35 Mutant Ebola Virus Lacks Virulence but Can Elicit Protective Immunity to Wild-Type Virus Challenge. Cell Rep. 2019;28:3032-3046.e6 pubmed publisher
Crifò B, Schaible B, Brown E, Halligan D, Scholz C, Fitzpatrick S, et al. Hydroxylase Inhibition Selectively Induces Cell Death in Monocytes. J Immunol. 2019;202:1521-1530 pubmed publisher
Hakimi A, Voss M, Kuo F, Sanchez A, Liu M, Nixon B, et al. Transcriptomic Profiling of the Tumor Microenvironment Reveals Distinct Subgroups of Clear Cell Renal Cell Cancer: Data from a Randomized Phase III Trial. Cancer Discov. 2019;9:510-525 pubmed publisher
Yang X, Chen Y, Li J, Chen L, Ren H, Liu Y, et al. Hypertonic saline maintains coagulofibrinolytic homeostasis following moderate‑to‑severe traumatic brain injury by regulating monocyte phenotype via expression of lncRNAs. Mol Med Rep. 2019;19:1083-1091 pubmed publisher
Azizi E, Carr A, Plitas G, Cornish A, Konopacki C, Prabhakaran S, et al. Single-Cell Map of Diverse Immune Phenotypes in the Breast Tumor Microenvironment. Cell. 2018;174:1293-1308.e36 pubmed publisher
Yeo L, Woodwyk A, Sood S, Lorenc A, Eichmann M, Pujol Autonell I, et al. Autoreactive T effector memory differentiation mirrors β cell function in type 1 diabetes. J Clin Invest. 2018;128:3460-3474 pubmed publisher
Maharaj S, Lu K, Radom Aizik S, Zaldivar F, Haddad F, Shin H, et al. Inter- and intra-subject variability of nitric oxide levels in leukocyte subpopulations. Nitric Oxide. 2018;72:41-45 pubmed publisher
Bowen E, Canfield J, Moore S, Hines M, Hartke B, Rademacher C. Predictors of CD4 health and viral suppression outcomes for formerly homeless people living with HIV/AIDS in scattered site supportive housing. AIDS Care. 2017;29:1458-1462 pubmed publisher
Palamides P, Jodeleit H, Föhlinger M, Beigel F, Herbach N, Mueller T, et al. A mouse model for ulcerative colitis based on NOD-scid IL2R ?null mice reconstituted with peripheral blood mononuclear cells from affected individuals. Dis Model Mech. 2016;9:985-97 pubmed publisher
Schrøder M, Melum G, Landsverk O, Bujko A, Yaqub S, Gran E, et al. CD1c-Expression by Monocytes - Implications for the Use of Commercial CD1c+ Dendritic Cell Isolation Kits. PLoS ONE. 2016;11:e0157387 pubmed publisher
Parameswaran R, Ramakrishnan P, Moreton S, Xia Z, Hou Y, Lee D, et al. Repression of GSK3 restores NK cell cytotoxicity in AML patients. Nat Commun. 2016;7:11154 pubmed publisher
Glaser K, Fehrholz M, Curstedt T, Kunzmann S, Speer C. Effects of the New Generation Synthetic Reconstituted Surfactant CHF5633 on Pro- and Anti-Inflammatory Cytokine Expression in Native and LPS-Stimulated Adult CD14+ Monocytes. PLoS ONE. 2016;11:e0146898 pubmed publisher
Teirlinck A, Roestenberg M, Bijker E, Hoffman S, Sauerwein R, Scholzen A. Plasmodium falciparum Infection of Human Volunteers Activates Monocytes and CD16+ Dendritic Cells and Induces Upregulation of CD16 and CD1c Expression. Infect Immun. 2015;83:3732-9 pubmed publisher
Radom Aizik S, Zaldivar F, Haddad F, Cooper D. Impact of brief exercise on circulating monocyte gene and microRNA expression: implications for atherosclerotic vascular disease. Brain Behav Immun. 2014;39:121-9 pubmed publisher
Reichwald K, Jørgensen T, Tougaard P, Skov S. TL1A induces TCR independent IL-6 and TNF-? production and growth of PLZF? leukocytes. PLoS ONE. 2014;9:e85793 pubmed publisher
Siegers G, Felizardo T, Mathieson A, Kosaka Y, Wang X, Medin J, et al. Anti-leukemia activity of in vitro-expanded human gamma delta T cells in a xenogeneic Ph+ leukemia model. PLoS ONE. 2011;6:e16700 pubmed publisher
Nguyen D, Ball E, Varki A. Myeloid precursors and acute myeloid leukemia cells express multiple CD33-related Siglecs. Exp Hematol. 2006;34:728-35 pubmed
product information
Antigen :
CD16
Apps. Abbrev. :
Flow Cytometry
Cat # :
302056
Clone :
3G8
Item :
PE anti-human CD16
Isotype :
Mouse IgG1, κ
Other Names :
FcγRIII, Fc gamma receptor, Fc gamma receptor 3
Size :
100 μg
Price (USD) :
244 USD
Reactivity :
Human, Cynomolgus, Rhesus
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
PE
Immunogen :
Human PMN cells
Application Notes :
The 3G8 antibody clone blocks neutrophil phagocytosis and stimulates NK cell proliferation. It has been reported that this clone interacts with the Fc RIIa and Fc RIIIb receptors causing neutrophil activation and aggregation 18 . Due to this phenomenon staining in whole blood may cause a reduction in the number of granulocytes or alter their scatter profile. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections 6 , immunoprecipitation 3 , stimulation of NK cell proliferation 4 , blocking of phagocytosis 5 , and blocking of immunoglobulin binding to Fc RIII 7,8 . The Ultra-LEAF purified antibody (Endotoxin 0.01 EU/ug, Azide-Free, 0.2 um filtered) is recommended for functional assays (Cat. No. 302049, 302050, 302057, 302058).
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA