Ultra-LEAF™ Purified anti-human CD16 | BioLegend 302049 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

Ultra-LEAF™ Purified anti-human CD16

catalog :

302049

quantity :

100 μg

price :

116 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

3G8

reactivity :

human, rhesus macaque

application :

neutralization, immunoprecipitation, flow cytometry, immunohistochemistry - frozen section, blocking or activating experiments

more info or order :

BioLegend product webpage

citations: 10

Published Application/Species/Sample/Dilution	Reference
blocking or activating experiments; human; 5 ug/ml; loading ...; fig s4	Zhu Y, Xie J, Shi J. Rac1/ROCK-driven membrane dynamics promote natural killer cell cytotoxicity via granzyme-induced necroptosis. BMC Biol. 2021;19:140 pubmed publisher
flow cytometry; human; loading ...; fig 4b	Muhammad F, Wang D, Montieth A, Lee S, Preble J, Foster C, et al. PD-1+ melanocortin receptor dependent-Treg cells prevent autoimmune disease. Sci Rep. 2019;9:16941 pubmed publisher
	Vidard L. 4-1BB and cytokines trigger human NK, γδ T, and CD8+ T cell proliferation and activation, but are not required for their effector functions. Immun Inflamm Dis. 2023;11:e749 pubmed publisher
	Cluff E, Magdaleno C, Fernandez E, House T, Swaminathan S, Varadaraj A, et al. Hypoxia-inducible factor-1 alpha expression is induced by IL-2 via the PI3K/mTOR pathway in hypoxic NK cells and supports effector functions in NKL cells and ex vivo expanded NK cells. Cancer Immunol Immunother. 2022;: pubmed publisher
	Gimpel A, Maccataio A, Unterweger H, Sokolova M, Schett G, Steffen U. IgA Complexes Induce Neutrophil Extracellular Trap Formation More Potently Than IgG Complexes. Front Immunol. 2021;12:761816 pubmed publisher
	Yamin R, Jones A, Hoffmann H, Schäfer A, Kao K, Francis R, et al. Fc-engineered antibody therapeutics with improved anti-SARS-CoV-2 efficacy. Nature. 2021;599:465-470 pubmed publisher
	Beasley A, Acheampong E, Lin W, Gray E. Multi-Marker Immunomagnetic Enrichment of Circulating Melanoma Cells. Methods Mol Biol. 2021;2265:213-222 pubmed publisher
	Zhu C, Song Z, Wang A, Srinivasan S, Yang G, Greco R, et al. Isatuximab Acts Through Fc-Dependent, Independent, and Direct Pathways to Kill Multiple Myeloma Cells. Front Immunol. 2020;11:1771 pubmed publisher
	Thulin N, Brewer R, Sherwood R, Bournazos S, Edwards K, Ramadoss N, et al. Maternal Anti-Dengue IgG Fucosylation Predicts Susceptibility to Dengue Disease in Infants. Cell Rep. 2020;31:107642 pubmed publisher
	Waight J, Chand D, Dietrich S, Gombos R, Horn T, Gonzalez A, et al. Selective FcγR Co-engagement on APCs Modulates the Activity of Therapeutic Antibodies Targeting T Cell Antigens. Cancer Cell. 2018;33:1033-1047.e5 pubmed publisher

product information

Antigen :

CD16

Apps. Abbrev. :

FC, CyTOF®, Block, IHC-F, IP, Stim

Cat # :

302049

Clone :

3G8

Item :

Ultra-LEAF™ Purified anti-human CD16

Isotype :

Mouse IgG1, κ

Other Names :

FcγRIII, Fc gamma receptor, Fc gamma receptor 3

Size :

100 μg

Price (USD) :

116 USD

Reactivity :

Human, Cynomolgus, Rhesus

Clonality :

Monoclonal

Host :

Mouse

Conjugate/Tag/Label :

ULEAF

Immunogen :

Human PMN cells

Application Notes :

The 3G8 antibody clone blocks neutrophil phagocytosis and stimulates NK cell proliferation. It has been reported that this clone interacts with the FcγRIIa and FcγRIIIb receptors causing neutrophil activation and aggregation^18. Due to this phenomenon staining in whole blood may cause a reduction in the number of granulocytes or alter their scatter profile.Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections^6, immunoprecipitation^3, stimulation of NK cell proliferation^4, blocking of phagocytosis^5, and blocking of immunoglobulin binding to FcγRIII^7,8. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 302049, 302050, 302057, 302058).;

more info or order :

BioLegend product webpage