product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Brilliant Violet 785™ anti-human CD16
catalog :
302045
quantity :
25 tests
price :
226 USD
clonality :
monoclonal
host :
mouse
conjugate :
Brilliant Violet 785
clone name :
3G8
reactivity :
human, rhesus macaque
application :
flow cytometry
more info or order :
citations: 27
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human
Bendall S, Simonds E, Qiu P, Amir E, Krutzik P, Finck R, et al. Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum. Science. 2011;332:687-96 pubmed publisher
Wheeler M, Stilp A, Rao S, Halld xf3 rsson B, Beyter D, Wen J, et al. Whole genome sequencing identifies structural variants contributing to hematologic traits in the NHLBI TOPMed program. Nat Commun. 2022;13:7592 pubmed publisher
Jung M, Aibaidula A, Brown D, Himes B, Cumba Garcia L, Parney I. Superinduction of immunosuppressive glioblastoma extracellular vesicles by IFN-γ through PD-L1 and IDO1. Neurooncol Adv. 2022;4:vdac017 pubmed publisher
Vyborova A, Janssen A, Gatti L, Karaiskaki F, Yonika A, van Dooremalen S, et al. γ9δ2 T-Cell Expansion and Phenotypic Profile Are Reflected in the CDR3δ Repertoire of Healthy Adults. Front Immunol. 2022;13:915366 pubmed publisher
Höfle J, Trenkner T, Kleist N, Schwane V, Vollmers S, Barcelona B, et al. Engagement of TRAIL triggers degranulation and IFNγ production in human natural killer cells. EMBO Rep. 2022;:e54133 pubmed publisher
Vijayakumar B, Boustani K, Ogger P, Papadaki A, Tonkin J, Orton C, et al. Immuno-proteomic profiling reveals aberrant immune cell regulation in the airways of individuals with ongoing post-COVID-19 respiratory disease. Immunity. 2022;55:542-556.e5 pubmed publisher
Qi S, Ngwa C, Morales Scheihing D, Al Mamun A, Ahnstedt H, Finger C, et al. Sex differences in the immune response to acute COVID-19 respiratory tract infection. Biol Sex Differ. 2021;12:66 pubmed publisher
Grace P, Dolatshahi S, Lu L, Cain A, Palmieri F, Petrone L, et al. Antibody Subclass and Glycosylation Shift Following Effective TB Treatment. Front Immunol. 2021;12:679973 pubmed publisher
Lee Y, O Brien L, Walpole C, Pearson F, Leal Rojas I, Masterman K, et al. Human CD141+ dendritic cells (cDC1) are impaired in patients with advanced melanoma but can be targeted to enhance anti-PD-1 in a humanized mouse model. J Immunother Cancer. 2021;9: pubmed publisher
Thompson E, Cascino K, Ordonez A, Zhou W, Vaghasia A, Hamacher Brady A, et al. Metabolic programs define dysfunctional immune responses in severe COVID-19 patients. Cell Rep. 2021;34:108863 pubmed publisher
Leylek R, Alcántara Hernández M, Granja J, Chavez M, Perez K, Diaz O, et al. Chromatin Landscape Underpinning Human Dendritic Cell Heterogeneity. Cell Rep. 2020;32:108180 pubmed publisher
Spitsin S, Schnepp B, Connell M, Liu T, Dang C, Pappa V, et al. Protection against SIV in Rhesus Macaques Using Albumin and CD4-Based Vector-Mediated Gene Transfer. Mol Ther Methods Clin Dev. 2020;17:1088-1096 pubmed publisher
Xu X, Davelaar N, Mus A, Asmawidjaja P, Hazes J, Baeten D, et al. Interleukin-17A Is Produced by CD4+ but Not CD8+ T Cells in Synovial Fluid Following T Cell Receptor Activation and Regulates Different Inflammatory Mediators Compared to Tumor Necrosis Factor in a Model of Psoriatic Arthritis Synovitis. Arthritis Rheumatol. 2020;72:1303-1313 pubmed publisher
Ferrando Martinez S, Huang K, Bennett A, Sterba P, Yu L, Suzich J, et al. HBeAg seroconversion is associated with a more effective PD-L1 blockade during chronic hepatitis B infection. JHEP Rep. 2019;1:170-178 pubmed publisher
Tran T, Guha R, Portugal S, Skinner J, Ongoiba A, Bhardwaj J, et al. A Molecular Signature in Blood Reveals a Role for p53 in Regulating Malaria-Induced Inflammation. Immunity. 2019;51:750-765.e10 pubmed publisher
Marquardt N, Kekäläinen E, Chen P, Lourda M, Wilson J, Scharenberg M, et al. Unique transcriptional and protein-expression signature in human lung tissue-resident NK cells. Nat Commun. 2019;10:3841 pubmed publisher
Zhu Y, Padgett L, Dinh H, Marcovecchio P, Blatchley A, Wu R, et al. Identification of an Early Unipotent Neutrophil Progenitor with Pro-tumoral Activity in Mouse and Human Bone Marrow. Cell Rep. 2018;24:2329-2341.e8 pubmed publisher
Martrus G, Kautz T, Lunemann S, Richert L, Glau L, Salzberger W, et al. Proliferative capacity exhibited by human liver-resident CD49a+CD25+ NK cells. PLoS ONE. 2017;12:e0182532 pubmed publisher
Korner C, Simoneau C, Schommers P, Granoff M, Ziegler M, Hölzemer A, et al. HIV-1-Mediated Downmodulation of HLA-C Impacts Target Cell Recognition and Antiviral Activity of NK Cells. Cell Host Microbe. 2017;22:111-119.e4 pubmed publisher
Marquardt N, Ivarsson M, Sundström E, Akesson E, Martini E, Eidsmo L, et al. Fetal CD103+ IL-17-Producing Group 3 Innate Lymphoid Cells Represent the Dominant Lymphocyte Subset in Human Amniotic Fluid. J Immunol. 2016;197:3069-3075 pubmed
Salzberger W, Garcia Beltran W, Dugan H, Gubbala S, Simoneau C, Gressens S, et al. Influence of Glycosylation Inhibition on the Binding of KIR3DL1 to HLA-B*57:01. PLoS ONE. 2015;10:e0145324 pubmed publisher
Marquardt N, Béziat V, Nyström S, Hengst J, Ivarsson M, Kekäläinen E, et al. Cutting edge: identification and characterization of human intrahepatic CD49a+ NK cells. J Immunol. 2015;194:2467-71 pubmed publisher
Wu Z, Sinzger C, Frascaroli G, Reichel J, Bayer C, Wang L, et al. Human cytomegalovirus-induced NKG2C(hi) CD57(hi) natural killer cells are effectors dependent on humoral antiviral immunity. J Virol. 2013;87:7717-25 pubmed publisher
Boltz A, Piater B, Toleikis L, Guenther R, Kolmar H, Hock B. Bi-specific aptamers mediating tumor cell lysis. J Biol Chem. 2011;286:21896-905 pubmed publisher
Brainard D, Seung E, Frahm N, Cariappa A, Bailey C, Hart W, et al. Induction of robust cellular and humoral virus-specific adaptive immune responses in human immunodeficiency virus-infected humanized BLT mice. J Virol. 2009;83:7305-21 pubmed publisher
Timmerman K, Flynn M, Coen P, Markofski M, Pence B. Exercise training-induced lowering of inflammatory (CD14+CD16+) monocytes: a role in the anti-inflammatory influence of exercise?. J Leukoc Biol. 2008;84:1271-8 pubmed publisher
Smed Sorensen A, Moll M, Cheng T, Lore K, Norlin A, Perbeck L, et al. IgG regulates the CD1 expression profile and lipid antigen-presenting function in human dendritic cells via FcgammaRIIa. Blood. 2008;111:5037-46 pubmed publisher
product information
Antigen :
CD16
Apps. Abbrev. :
FC
Cat # :
302045
Clone :
3G8
Item :
Brilliant Violet 785™ anti-human CD16
Isotype :
Mouse IgG1, κ
Other Names :
FcγRIII, Fc gamma receptor, Fc gamma receptor 3
Size :
25 tests
Price (USD) :
226 USD
Reactivity :
Human, Cynomolgus, Rhesus
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
BV785
Immunogen :
Human PMN cells
Application Notes :
The 3G8 antibody clone blocks neutrophil phagocytosis and stimulates NK cell proliferation. It has been reported that this clone interacts with the FcγRIIa and FcγRIIIb receptors causing neutrophil activation and aggregation^18. Due to this phenomenon staining in whole blood may cause a reduction in the number of granulocytes or alter their scatter profile.Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections^6, immunoprecipitation^3, stimulation of NK cell proliferation^4, blocking of phagocytosis^5, and blocking of immunoglobulin binding to FcγRIII^7,8. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 302049, 302050, 302057, 302058).;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA