Brilliant Violet 421™ anti-human CD16 | BioLegend 302038 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

Brilliant Violet 421™ anti-human CD16

catalog :

302038

quantity :

100 tests

price :

381 USD

clonality :

monoclonal

host :

mouse

conjugate :

BV421

clone name :

3G8

reactivity :

human, rhesus macaque

application :

flow cytometry

more info or order :

BioLegend product webpage

citations: 15

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; loading ...; fig 5e	Kaminski M, Bendzick L, Hopps R, Kauffman M, Kodal B, Soignier Y, et al. TEM8 Tri-specific Killer Engager binds both tumor and tumor stroma to specifically engage natural killer cell anti-tumor activity. J Immunother Cancer. 2022;10: pubmed publisher
flow cytometry; human	Bendall S, Simonds E, Qiu P, Amir E, Krutzik P, Finck R, et al. Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum. Science. 2011;332:687-96 pubmed publisher
	Chakraborty S, González J, Sievers B, Mallajosyula V, Chakraborty S, Dubey M, et al. Early non-neutralizing, afucosylated antibody responses are associated with COVID-19 severity. Sci Transl Med. 2022;14:eabm7853 pubmed publisher
	Dhariwala M, Karthikeyan D, Vasquez K, Farhat S, Weckel A, Taravati K, et al. Developing Human Skin Contains Lymphocytes Demonstrating a Memory Signature. Cell Rep Med. 2020;1:100132 pubmed publisher
	Ols S, Yang L, Thompson E, Pushparaj P, Tran K, Liang F, et al. Route of Vaccine Administration Alters Antigen Trafficking but Not Innate or Adaptive Immunity. Cell Rep. 2020;30:3964-3971.e7 pubmed publisher
	D Antoni M, Kallianpur K, Premeaux T, Corley M, Fujita T, Laws E, et al. Lower Interferon Regulatory Factor-8 Expression in Peripheral Myeloid Cells Tracks With Adverse Central Nervous System Outcomes in Treated HIV Infection. Front Immunol. 2019;10:2789 pubmed publisher
	de Andrade L, Lu Y, Luoma A, Ito Y, Pan D, Pyrdol J, et al. Discovery of specialized NK cell populations infiltrating human melanoma metastases. JCI Insight. 2019;4: pubmed publisher
	Martínez Vicente P, Farré D, Sanchez C, Alcami A, Engel P, Angulo A. Subversion of natural killer cell responses by a cytomegalovirus-encoded soluble CD48 decoy receptor. PLoS Pathog. 2019;15:e1007658 pubmed publisher
	Upadhyay A, Kauffman R, Wolabaugh A, Cho A, Patel N, Reiss S, et al. BALDR: a computational pipeline for paired heavy and light chain immunoglobulin reconstruction in single-cell RNA-seq data. Genome Med. 2018;10:20 pubmed publisher
	Schofield L, Ioannidis L, Karl S, Robinson L, Tan Q, Poole D, et al. Synergistic effect of IL-12 and IL-18 induces TIM3 regulation of ?? T cell function and decreases the risk of clinical malaria in children living in Papua New Guinea. BMC Med. 2017;15:114 pubmed publisher
	Wu Z, Sinzger C, Frascaroli G, Reichel J, Bayer C, Wang L, et al. Human cytomegalovirus-induced NKG2C(hi) CD57(hi) natural killer cells are effectors dependent on humoral antiviral immunity. J Virol. 2013;87:7717-25 pubmed publisher
	Boltz A, Piater B, Toleikis L, Guenther R, Kolmar H, Hock B. Bi-specific aptamers mediating tumor cell lysis. J Biol Chem. 2011;286:21896-905 pubmed publisher
	Brainard D, Seung E, Frahm N, Cariappa A, Bailey C, Hart W, et al. Induction of robust cellular and humoral virus-specific adaptive immune responses in human immunodeficiency virus-infected humanized BLT mice. J Virol. 2009;83:7305-21 pubmed publisher
	Timmerman K, Flynn M, Coen P, Markofski M, Pence B. Exercise training-induced lowering of inflammatory (CD14+CD16+) monocytes: a role in the anti-inflammatory influence of exercise?. J Leukoc Biol. 2008;84:1271-8 pubmed publisher
	Smed Sorensen A, Moll M, Cheng T, Lore K, Norlin A, Perbeck L, et al. IgG regulates the CD1 expression profile and lipid antigen-presenting function in human dendritic cells via FcgammaRIIa. Blood. 2008;111:5037-46 pubmed publisher

product information

Antigen :

CD16

Apps. Abbrev. :

Flow Cytometry

Cat # :

302038

Clone :

3G8

Item :

Brilliant Violet 421™ anti-human CD16

Isotype :

Mouse IgG1, κ

Other Names :

FcγRIII, Fc gamma receptor, Fc gamma receptor 3

Size :

100 tests

Price (USD) :

381 USD

Reactivity :

Human, Cynomolgus, Rhesus

Clonality :

Monoclonal

Host :

Mouse

Conjugate/Tag/Label :

BV421

Immunogen :

Human PMN cells

Application Notes :

The 3G8 antibody clone blocks neutrophil phagocytosis and stimulates NK cell proliferation. It has been reported that this clone interacts with the Fc RIIa and Fc RIIIb receptors causing neutrophil activation and aggregation 18 . Due to this phenomenon staining in whole blood may cause a reduction in the number of granulocytes or alter their scatter profile. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections 6 , immunoprecipitation 3 , stimulation of NK cell proliferation 4 , blocking of phagocytosis 5 , and blocking of immunoglobulin binding to Fc RIII 7,8 . The Ultra-LEAF purified antibody (Endotoxin 0.01 EU/ug, Azide-Free, 0.2 um filtered) is recommended for functional assays (Cat. No. 302049, 302050, 302057, 302058).

more info or order :

BioLegend product webpage