product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Brilliant Violet 570™ anti-human CD16
catalog :
302035
quantity :
25 tests
price :
226 USD
clonality :
monoclonal
host :
mouse
conjugate :
Brilliant Violet 570
clone name :
3G8
reactivity :
human, mouse, rhesus macaque
application :
flow cytometry
more info or order :
citations: 20
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; loading ...
Li D, Edwards R, Manne K, Martinez D, Schäfer A, Alam S, et al. In vitro and in vivo functions of SARS-CoV-2 infection-enhancing and neutralizing antibodies. Cell. 2021;184:4203-4219.e32 pubmed publisher
  • flow cytometry; human; loading ...; fig 2a
Dosch M, Zindel J, Jebbawi F, Melin N, Sánchez Taltavull D, Stroka D, et al. Connexin-43-dependent ATP release mediates macrophage activation during sepsis. elife. 2019;8: pubmed publisher
  • flow cytometry; mouse
Amos J, Himes J, Armand L, Gurley T, Martinez D, Colvin L, et al. Rapid Development of gp120-Focused Neutralizing B Cell Responses during Acute Simian Immunodeficiency Virus Infection of African Green Monkeys. J Virol. 2015;89:9485-98 pubmed publisher
  • flow cytometry; human
Bendall S, Simonds E, Qiu P, Amir E, Krutzik P, Finck R, et al. Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum. Science. 2011;332:687-96 pubmed publisher
Obradovic A, Chowdhury N, Haake S, Ager C, Wang V, Vlahos L, et al. Single-cell protein activity analysis identifies recurrence-associated renal tumor macrophages. Cell. 2021;184:2988-3005.e16 pubmed publisher
Mathewson N, Ashenberg O, Tirosh I, Gritsch S, Perez E, Marx S, et al. Inhibitory CD161 receptor identified in glioma-infiltrating T cells by single-cell analysis. Cell. 2021;184:1281-1298.e26 pubmed publisher
Pasciuto E, Burton O, Roca C, Lagou V, Rajan W, Theys T, et al. Microglia Require CD4 T Cells to Complete the Fetal-to-Adult Transition. Cell. 2020;182:625-640.e24 pubmed publisher
Han Q, Bradley T, Williams W, Cain D, Montefiori D, Saunders K, et al. Neonatal Rhesus Macaques Have Distinct Immune Cell Transcriptional Profiles following HIV Envelope Immunization. Cell Rep. 2020;30:1553-1569.e6 pubmed publisher
Martin Gayo E, Gao C, Chen H, Ouyang Z, Kim D, Kolb K, et al. Immunological Fingerprints of Controllers Developing Neutralizing HIV-1 Antibodies. Cell Rep. 2020;30:984-996.e4 pubmed publisher
Meehan S, Kolyagin G, Parks D, Youngyunpipatkul J, Herzenberg L, Walther G, et al. Automated subset identification and characterization pipeline for multidimensional flow and mass cytometry data clustering and visualization. Commun Biol. 2019;2:229 pubmed publisher
Bradley T, Peppa D, Pedroza Pacheco I, Li D, Cain D, Henao R, et al. RAB11FIP5 Expression and Altered Natural Killer Cell Function Are Associated with Induction of HIV Broadly Neutralizing Antibody Responses. Cell. 2018;175:387-399.e17 pubmed publisher
Aliota M, Dudley D, Newman C, Mohr E, Gellerup D, Breitbach M, et al. Heterologous Protection against Asian Zika Virus Challenge in Rhesus Macaques. PLoS Negl Trop Dis. 2016;10:e0005168 pubmed publisher
Dudley D, Aliota M, Mohr E, Weiler A, Lehrer Brey G, Weisgrau K, et al. A rhesus macaque model of Asian-lineage Zika virus infection. Nat Commun. 2016;7:12204 pubmed publisher
Santra S, Tomaras G, Warrier R, Nicely N, Liao H, Pollara J, et al. Human Non-neutralizing HIV-1 Envelope Monoclonal Antibodies Limit the Number of Founder Viruses during SHIV Mucosal Infection in Rhesus Macaques. PLoS Pathog. 2015;11:e1005042 pubmed publisher
Stanisic D, Cutts J, Eriksson E, Fowkes F, Rosanas Urgell A, Siba P, et al. ?? T cells and CD14+ monocytes are predominant cellular sources of cytokines and chemokines associated with severe malaria. J Infect Dis. 2014;210:295-305 pubmed publisher
Wu Z, Sinzger C, Frascaroli G, Reichel J, Bayer C, Wang L, et al. Human cytomegalovirus-induced NKG2C(hi) CD57(hi) natural killer cells are effectors dependent on humoral antiviral immunity. J Virol. 2013;87:7717-25 pubmed publisher
Boltz A, Piater B, Toleikis L, Guenther R, Kolmar H, Hock B. Bi-specific aptamers mediating tumor cell lysis. J Biol Chem. 2011;286:21896-905 pubmed publisher
Brainard D, Seung E, Frahm N, Cariappa A, Bailey C, Hart W, et al. Induction of robust cellular and humoral virus-specific adaptive immune responses in human immunodeficiency virus-infected humanized BLT mice. J Virol. 2009;83:7305-21 pubmed publisher
Timmerman K, Flynn M, Coen P, Markofski M, Pence B. Exercise training-induced lowering of inflammatory (CD14+CD16+) monocytes: a role in the anti-inflammatory influence of exercise?. J Leukoc Biol. 2008;84:1271-8 pubmed publisher
Smed Sorensen A, Moll M, Cheng T, Lore K, Norlin A, Perbeck L, et al. IgG regulates the CD1 expression profile and lipid antigen-presenting function in human dendritic cells via FcgammaRIIa. Blood. 2008;111:5037-46 pubmed publisher
product information
Antigen :
CD16
Apps. Abbrev. :
Flow Cytometry
Cat # :
302035
Clone :
3G8
Item :
Brilliant Violet 570™ anti-human CD16
Isotype :
Mouse IgG1, κ
Other Names :
FcγRIII, Fc gamma receptor, Fc gamma receptor 3
Size :
25 tests
Price (USD) :
226 USD
Reactivity :
Human, Cynomolgus, Rhesus
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
BV570
Immunogen :
Human PMN cells
Application Notes :
The 3G8 antibody clone blocks neutrophil phagocytosis and stimulates NK cell proliferation. It has been reported that this clone interacts with the Fc RIIa and Fc RIIIb receptors causing neutrophil activation and aggregation 18 . Due to this phenomenon staining in whole blood may cause a reduction in the number of granulocytes or alter their scatter profile. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections 6 , immunoprecipitation 3 , stimulation of NK cell proliferation 4 , blocking of phagocytosis 5 , and blocking of immunoglobulin binding to Fc RIII 7,8 . The Ultra-LEAF purified antibody (Endotoxin 0.01 EU/ug, Azide-Free, 0.2 um filtered) is recommended for functional assays (Cat. No. 302049, 302050, 302057, 302058).
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA