LEAF™ Purified anti-human CD16 | BioLegend 302033 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

LEAF™ Purified anti-human CD16

catalog :

302033

quantity :

1 mg

price :

640 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

3G8

reactivity :

human, mouse, rhesus macaque

application :

immunohistochemistry, neutralization, immunoprecipitation, flow cytometry, immunohistochemistry - frozen section

citations: 24

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; loading ...; fig 4b	Muhammad F, Wang D, Montieth A, Lee S, Preble J, Foster C, et al. PD-1+ melanocortin receptor dependent-Treg cells prevent autoimmune disease. Sci Rep. 2019;9:16941 pubmed publisher
flow cytometry; rhesus macaque; loading ...; fig 7a	Kasturi S, Kozlowski P, Nakaya H, Burger M, Russo P, Pham M, et al. Adjuvanting a Simian Immunodeficiency Virus Vaccine with Toll-Like Receptor Ligands Encapsulated in Nanoparticles Induces Persistent Antibody Responses and Enhanced Protection in TRIM5α Restrictive Macaques. J Virol. 2017;91: pubmed publisher
flow cytometry; human; loading ...	Demers K, Makedonas G, Buggert M, Eller M, Ratcliffe S, Goonetilleke N, et al. Temporal Dynamics of CD8+ T Cell Effector Responses during Primary HIV Infection. PLoS Pathog. 2016;12:e1005805 pubmed publisher
flow cytometry; mouse; 1:200	Ludigs K, Jandus C, Utzschneider D, Staehli F, Bessoles S, Dang A, et al. NLRC5 shields T lymphocytes from NK-cell-mediated elimination under inflammatory conditions. Nat Commun. 2016;7:10554 pubmed publisher
flow cytometry; human; fig 5	Westman J, Papareddy P, Dahlgren M, Chakrakodi B, Norrby Teglund A, Smeds E, et al. Extracellular Histones Induce Chemokine Production in Whole Blood Ex Vivo and Leukocyte Recruitment In Vivo. PLoS Pathog. 2015;11:e1005319 pubmed publisher
flow cytometry; human; fig 7	Venkatasubramanian S, Tripathi D, Tucker T, Paidipally P, Cheekatla S, Welch E, et al. Tissue factor expression by myeloid cells contributes to protective immune response against Mycobacterium tuberculosis infection. Eur J Immunol. 2016;46:464-79 pubmed publisher
flow cytometry; human; loading ...; fig s1b	Schulz A, MÃ¤lzer J, Domingo C, JÃ¼rchott K, GrÃ¼tzkau A, Babel N, et al. Low Thymic Activity and Dendritic Cell Numbers Are Associated with the Immune Response to Primary Viral Infection in Elderly Humans. J Immunol. 2015;195:4699-711 pubmed publisher
flow cytometry; mouse	Amos J, Himes J, Armand L, Gurley T, Martinez D, Colvin L, et al. Rapid Development of gp120-Focused Neutralizing B Cell Responses during Acute Simian Immunodeficiency Virus Infection of African Green Monkeys. J Virol. 2015;89:9485-98 pubmed publisher
flow cytometry; human; fig s3	Mende N, Kuchen E, Lesche M, Grinenko T, Kokkaliaris K, Hanenberg H, et al. CCND1-CDK4-mediated cell cycle progression provides a competitive advantage for human hematopoietic stem cells in vivo. J Exp Med. 2015;212:1171-83 pubmed publisher
flow cytometry; human; fig 1	O Brien E, Abdulahad W, Rutgers A, Huitema M, O Reilly V, Coughlan A, et al. Intermediate monocytes in ANCA vasculitis: increased surface expression of ANCA autoantigens and IL-1Î² secretion in response to anti-MPO antibodies. Sci Rep. 2015;5:11888 pubmed publisher
flow cytometry; human; fig 4	Weldon A, Moldovan I, Cabling M, Hernandez E, Hsu S, Gonzalez J, et al. Surface APRIL Is Elevated on Myeloid Cells and Is Associated with Disease Activity in Patients with Rheumatoid Arthritis. J Rheumatol. 2015;42:749-59 pubmed publisher
flow cytometry; human; fig 1	HÃ¼nniger K, Bieber K, Martin R, Lehnert T, Figge M, LÃ¶ffler J, et al. A second stimulus required for enhanced antifungal activity of human neutrophils in blood is provided by anaphylatoxin C5a. J Immunol. 2015;194:1199-210 pubmed publisher
flow cytometry; human; fig 7	Hartung E, Becker M, Bachem A, Reeg N, JÃ¤kel A, Hutloff A, et al. Induction of potent CD8 T cell cytotoxicity by specific targeting of antigen to cross-presenting dendritic cells in vivo via murine or human XCR1. J Immunol. 2015;194:1069-79 pubmed publisher
flow cytometry; human	Presnell S, Al Attar A, Cichocki F, Miller J, Lutz C. Human natural killer cell microRNA: differential expression of MIR181A1B1 and MIR181A2B2 genes encoding identical mature microRNAs. Genes Immun. 2015;16:89-98 pubmed publisher
flow cytometry; human	Mandl M, Schmitz S, Weber C, Hristov M. Characterization of the CD14++CD16+ monocyte population in human bone marrow. PLoS ONE. 2014;9:e112140 pubmed publisher
flow cytometry; rhesus macaque immunohistochemistry; rhesus macaque	Holder G, McGary C, Johnson E, Zheng R, John V, Sugimoto C, et al. Expression of the mannose receptor CD206 in HIV and SIV encephalitis: a phenotypic switch of brain perivascular macrophages with virus infection. J Neuroimmune Pharmacol. 2014;9:716-26 pubmed publisher
flow cytometry; human	Wiernik A, Foley B, Zhang B, Verneris M, Warlick E, Gleason M, et al. Targeting natural killer cells to acute myeloid leukemia in vitro with a CD16 x 33 bispecific killer cell engager and ADAM17 inhibition. Clin Cancer Res. 2013;19:3844-55 pubmed publisher
flow cytometry; human	Bendall S, Simonds E, Qiu P, Amir E, Krutzik P, Finck R, et al. Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum. Science. 2011;332:687-96 pubmed publisher
	Gruber C, Calis J, Buta S, Evrony G, Martin J, Uhl S, et al. Complex Autoinflammatory Syndrome Unveils Fundamental Principles of JAK1 Kinase Transcriptional and Biochemical Function. Immunity. 2020;53:672-684.e11 pubmed publisher
	Martin J, Chang C, Boschetti G, Ungaro R, Giri M, Grout J, et al. Single-Cell Analysis of Crohn's Disease Lesions Identifies a Pathogenic Cellular Module Associated with Resistance to Anti-TNF Therapy. Cell. 2019;178:1493-1508.e20 pubmed publisher
	Vidard L, Dureuil C, Baudhuin J, Vescovi L, Durand L, Sierra V, et al. CD137 (4-1BB) Engagement Fine-Tunes Synergistic IL-15- and IL-21-Driven NK Cell Proliferation. J Immunol. 2019;: pubmed publisher
	Rölle A, Meyer M, Calderazzo S, Jager D, Momburg F. Distinct HLA-E Peptide Complexes Modify Antibody-Driven Effector Functions of Adaptive NK Cells. Cell Rep. 2018;24:1967-1976.e4 pubmed publisher
	van der Heijden J, Breunis W, Geissler J, de Boer M, van den Berg T, Kuijpers T. Phenotypic variation in IgG receptors by nonclassical FCGR2C alleles. J Immunol. 2012;188:1318-24 pubmed publisher
	Goodridge J, Lathbury L, John E, Charles A, Christiansen F, Witt C. The genotype of the NK cell receptor, KIR2DL4, influences INFgamma secretion by decidual natural killer cells. Mol Hum Reprod. 2009;15:489-97 pubmed publisher

product information

Antigen :

CD16

Apps. Abbrev. :

Flow Cytometry, CyTOF®, Block, IHC-F, IP, Stim

Cat # :

302033

Clone :

3G8

Item :

LEAF™ Purified anti-human CD16

Isotype :

Mouse IgG1, κ

Other Names :

FcγRIII

Size :

1 mg

Price (USD) :

640 USD

Reactivity :

Human, African Green, Baboon, Capuchin Monkey, Chimpanzee, Cynomolgus, Marmoset, Pigtailed Macaque, Rhesus, Sooty Mangabey, Squirrel Monkey

Clonality :

Monoclonal

Host :

Mouse

Conjugate/Tag/Label :

LEAF

Immunogen :

Human PMN cells

Application Notes :

The 3G8 antibody clone blocks neutrophil phagocytosis and stimulates NK cell proliferation. It has been reported that this clone interacts with the Fc?RIIa and Fc?RIIIb receptors causing neutrophil activation and aggregation 18 . Due to this phenomenon staining in whole blood may cause a reduction in the number of granulocytes or alter their scatter profile. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections 6 , immunoprecipitation 3 , stimulation of NK cell proliferation 4 , blocking of phagocytosis 5 , and blocking of immunoglobulin binding to Fc?RIII 7,8 . The LEAF purified antibody (Endotoxin 0.1 EU/ug, Azide-Free, 0.2 um filtered) is recommended for functional assays (Cat. No. 302014). For highly sensitive assays, we recommend Ultra-LEAF purified antibody (Cat. No. 302050) with a lower endotoxin limit than standard LEAF purified antibodies (Endotoxin 0.01 EU/ug).