PerCP anti-human CD16 | BioLegend 302030 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

PerCP anti-human CD16

catalog :

302030

quantity :

100 tests

price :

335 USD

clonality :

monoclonal

host :

mouse

conjugate :

PerCP

clone name :

3G8

reactivity :

human, rhesus macaque

application :

flow cytometry

more info or order :

BioLegend product webpage

citations: 15

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human	Bendall S, Simonds E, Qiu P, Amir E, Krutzik P, Finck R, et al. Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum. Science. 2011;332:687-96 pubmed publisher
	Tarke A, Coelho C, Zhang Z, Dan J, Yu E, Methot N, et al. SARS-CoV-2 vaccination induces immunological T cell memory able to cross-recognize variants from Alpha to Omicron. Cell. 2022;185:847-859.e11 pubmed publisher
	Sefik E, Israelow B, Mirza H, Zhao J, Qu R, Kaffe E, et al. A humanized mouse model of chronic COVID-19. Nat Biotechnol. 2021;: pubmed publisher
	Iwabuchi R, Ide K, Terahara K, Wagatsuma R, Iwaki R, Matsunaga H, et al. Development of an Inflammatory CD14+ Dendritic Cell Subset in Humanized Mice. Front Immunol. 2021;12:643040 pubmed publisher
	Wang L, Wang H, Chiang H, Huang L, Chiu S, Siu L, et al. Human Placental MSC-Secreted IL-1β Enhances Neutrophil Bactericidal Functions during Hypervirulent Klebsiella Infection. Cell Rep. 2020;32:108188 pubmed publisher
	Dinh H, Eggert T, Meyer M, Zhu Y, Olingy C, Llewellyn R, et al. Coexpression of CD71 and CD117 Identifies an Early Unipotent Neutrophil Progenitor Population in Human Bone Marrow. Immunity. 2020;53:319-334.e6 pubmed publisher
	Takeda A, Hollmen M, Dermadi D, Pan J, Brulois K, Kaukonen R, et al. Single-Cell Survey of Human Lymphatics Unveils Marked Endothelial Cell Heterogeneity and Mechanisms of Homing for Neutrophils. Immunity. 2019;51:561-572.e5 pubmed publisher
	Bradley T, Peppa D, Pedroza Pacheco I, Li D, Cain D, Henao R, et al. RAB11FIP5 Expression and Altered Natural Killer Cell Function Are Associated with Induction of HIV Broadly Neutralizing Antibody Responses. Cell. 2018;175:387-399.e17 pubmed publisher
	Clark L, Mahmutovic S, Raymond D, Dilanyan T, Koma T, Manning J, et al. Vaccine-elicited receptor-binding site antibodies neutralize two New World hemorrhagic fever arenaviruses. Nat Commun. 2018;9:1884 pubmed publisher
	Wu Z, Sinzger C, Frascaroli G, Reichel J, Bayer C, Wang L, et al. Human cytomegalovirus-induced NKG2C(hi) CD57(hi) natural killer cells are effectors dependent on humoral antiviral immunity. J Virol. 2013;87:7717-25 pubmed publisher
	Perros F, Cohen Kaminsky S, Gambaryan N, Girerd B, Raymond N, Klingelschmitt I, et al. Cytotoxic cells and granulysin in pulmonary arterial hypertension and pulmonary veno-occlusive disease. Am J Respir Crit Care Med. 2013;187:189-96 pubmed publisher
	Boltz A, Piater B, Toleikis L, Guenther R, Kolmar H, Hock B. Bi-specific aptamers mediating tumor cell lysis. J Biol Chem. 2011;286:21896-905 pubmed publisher
	Brainard D, Seung E, Frahm N, Cariappa A, Bailey C, Hart W, et al. Induction of robust cellular and humoral virus-specific adaptive immune responses in human immunodeficiency virus-infected humanized BLT mice. J Virol. 2009;83:7305-21 pubmed publisher
	Timmerman K, Flynn M, Coen P, Markofski M, Pence B. Exercise training-induced lowering of inflammatory (CD14+CD16+) monocytes: a role in the anti-inflammatory influence of exercise?. J Leukoc Biol. 2008;84:1271-8 pubmed publisher
	Smed Sorensen A, Moll M, Cheng T, Lore K, Norlin A, Perbeck L, et al. IgG regulates the CD1 expression profile and lipid antigen-presenting function in human dendritic cells via FcgammaRIIa. Blood. 2008;111:5037-46 pubmed publisher

product information

Antigen :

CD16

Apps. Abbrev. :

Flow Cytometry

Cat # :

302030

Clone :

3G8

Item :

PerCP anti-human CD16

Isotype :

Mouse IgG1, κ

Other Names :

FcγRIII, Fc gamma receptor, Fc gamma receptor 3

Size :

100 tests

Price (USD) :

335 USD

Reactivity :

Human, Cynomolgus, Rhesus

Clonality :

Monoclonal

Host :

Mouse

Conjugate/Tag/Label :

PERCP

Immunogen :

Human PMN cells

Application Notes :

The 3G8 antibody clone blocks neutrophil phagocytosis and stimulates NK cell proliferation. It has been reported that this clone interacts with the Fc RIIa and Fc RIIIb receptors causing neutrophil activation and aggregation 18 . Due to this phenomenon staining in whole blood may cause a reduction in the number of granulocytes or alter their scatter profile. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections 6 , immunoprecipitation 3 , stimulation of NK cell proliferation 4 , blocking of phagocytosis 5 , and blocking of immunoglobulin binding to Fc RIII 7,8 . The Ultra-LEAF purified antibody (Endotoxin 0.01 EU/ug, Azide-Free, 0.2 um filtered) is recommended for functional assays (Cat. No. 302049, 302050, 302057, 302058).

more info or order :

BioLegend product webpage