product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Alexa Fluor® 700 anti-human CD16
catalog :
302026
quantity :
100 μg
price :
268 USD
clonality :
monoclonal
host :
mouse
conjugate :
AF700
clone name :
3G8
reactivity :
human, rhesus macaque
application :
flow cytometry, blocking or activating experiments
more info or order :
citations: 33
Published Application/Species/Sample/DilutionReference
  • blocking or activating experiments; human; 5 ug/ml; loading ...; fig s4
Zhu Y, Xie J, Shi J. Rac1/ROCK-driven membrane dynamics promote natural killer cell cytotoxicity via granzyme-induced necroptosis. BMC Biol. 2021;19:140 pubmed publisher
  • flow cytometry; human; loading ...; fig 4b
Muhammad F, Wang D, Montieth A, Lee S, Preble J, Foster C, et al. PD-1+ melanocortin receptor dependent-Treg cells prevent autoimmune disease. Sci Rep. 2019;9:16941 pubmed publisher
  • flow cytometry; rhesus macaque; loading ...; fig 7a
Kasturi S, Kozlowski P, Nakaya H, Burger M, Russo P, Pham M, et al. Adjuvanting a Simian Immunodeficiency Virus Vaccine with Toll-Like Receptor Ligands Encapsulated in Nanoparticles Induces Persistent Antibody Responses and Enhanced Protection in TRIM5α Restrictive Macaques. J Virol. 2017;91: pubmed publisher
  • flow cytometry; human
Chalan P, Bijzet J, Huitema M, Kroesen B, Brouwer E, Boots A. Expression of Lectin-Like Transcript 1, the Ligand for CD161, in Rheumatoid Arthritis. PLoS ONE. 2015;10:e0132436 pubmed publisher
  • flow cytometry; rhesus macaque; fig 3
Chowdhury A, Hayes T, Bosinger S, Lawson B, Vanderford T, Schmitz J, et al. Differential Impact of In Vivo CD8+ T Lymphocyte Depletion in Controller versus Progressor Simian Immunodeficiency Virus-Infected Macaques. J Virol. 2015;89:8677-86 pubmed publisher
  • flow cytometry; human
Bendall S, Simonds E, Qiu P, Amir E, Krutzik P, Finck R, et al. Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum. Science. 2011;332:687-96 pubmed publisher
. A blood atlas of COVID-19 defines hallmarks of disease severity and specificity. Cell. 2022;185:916-938.e58 pubmed publisher
Georg P, Astaburuaga García R, Bonaguro L, Brumhard S, Michalick L, Lippert L, et al. Complement activation induces excessive T cell cytotoxicity in severe COVID-19. Cell. 2022;185:493-512.e25 pubmed publisher
Cluff E, Magdaleno C, Fernandez E, House T, Swaminathan S, Varadaraj A, et al. Hypoxia-inducible factor-1 alpha expression is induced by IL-2 via the PI3K/mTOR pathway in hypoxic NK cells and supports effector functions in NKL cells and ex vivo expanded NK cells. Cancer Immunol Immunother. 2022;: pubmed publisher
Torres Ruiz J, Pérez Fragoso A, Maravillas Montero J, Llorente L, Mejía Domínguez N, Páez Franco J, et al. Redefining COVID-19 Severity and Prognosis: The Role of Clinical and Immunobiotypes. Front Immunol. 2021;12:689966 pubmed publisher
Forsberg M, Kink J, Thickens A, Lewis B, Childs C, Hematti P, et al. Exosomes from primed MSCs can educate monocytes as a cellular therapy for hematopoietic acute radiation syndrome. Stem Cell Res Ther. 2021;12:459 pubmed publisher
Portillo A, Hogg R, Poznanski S, Rojas E, Cashell N, Hammill J, et al. Expanded human NK cells armed with CAR uncouple potent anti-tumor activity from off-tumor toxicity against solid tumors. iScience. 2021;24:102619 pubmed publisher
Miao L, Zhuo Z, Tang J, Huang X, Liu J, Wang H, et al. FABP4 deactivates NF-κB-IL1α pathway by ubiquitinating ATPB in tumor-associated macrophages and promotes neuroblastoma progression. Clin Transl Med. 2021;11:e395 pubmed publisher
Veenhuis R, Williams D, Shirk E, Abreu C, Ferreira E, Coughlin J, et al. Higher circulating intermediate monocytes are associated with cognitive function in women with HIV. JCI Insight. 2021;6: pubmed publisher
Hearnden R, Sandhar B, Vyas V, Longhi M. Isolation of stromal vascular fraction cell suspensions from mouse and human adipose tissues for downstream applications. STAR Protoc. 2021;2:100422 pubmed publisher
Koutsakos M, Rowntree L, Hensen L, Chua B, van de Sandt C, Habel J, et al. Integrated immune dynamics define correlates of COVID-19 severity and antibody responses. Cell Rep Med. 2021;2:100208 pubmed publisher
Vanoni G, Ercolano G, Candiani S, Rutigliani M, Lanata M, Derre L, et al. Human primed ILCPs support endothelial activation through NF-κB signaling. elife. 2021;10: pubmed publisher
Rao S, Yao Y, Soares de Brito J, Yao Q, Shen A, Watkinson R, et al. Dissecting ELANE neutropenia pathogenicity by human HSC gene editing. Cell Stem Cell. 2021;: pubmed publisher
Cytlak U, Resteu A, Pagan S, Green K, Milne P, Maisuria S, et al. Differential IRF8 Transcription Factor Requirement Defines Two Pathways of Dendritic Cell Development in Humans. Immunity. 2020;53:353-370.e8 pubmed publisher
Pham T, Meziane O, Miah M, Volodina O, Colas C, Beland K, et al. Flt3L-Mediated Expansion of Plasmacytoid Dendritic Cells Suppresses HIV Infection in Humanized Mice. Cell Rep. 2019;29:2770-2782.e5 pubmed publisher
Mastelic Gavillet B, Navarro Rodrigo B, Décombaz L, Wang H, Ercolano G, Ahmed R, et al. Adenosine mediates functional and metabolic suppression of peripheral and tumor-infiltrating CD8+ T cells. J Immunother Cancer. 2019;7:257 pubmed publisher
de Jonge K, Ebering A, Nassiri S, Maby El Hajjami H, Ouertatani Sakouhi H, Baumgaertner P, et al. Circulating CD56bright NK cells inversely correlate with survival of melanoma patients. Sci Rep. 2019;9:4487 pubmed publisher
Tognarelli S, Wirsching S, von Metzler I, Rais B, Jacobs B, Serve H, et al. Enhancing the Activation and Releasing the Brakes: A Double Hit Strategy to Improve NK Cell Cytotoxicity Against Multiple Myeloma. Front Immunol. 2018;9:2743 pubmed publisher
Poznanski S, Nham T, Chew M, Lee A, Hammill J, Fan I, et al. Expanded CD56superbrightCD16+ NK Cells from Ovarian Cancer Patients Are Cytotoxic against Autologous Tumor in a Patient-Derived Xenograft Murine Model. Cancer Immunol Res. 2018;6:1174-1185 pubmed publisher
Lusty E, Poznanski S, Kwofie K, Mandur T, Lee D, Richards C, et al. IL-18/IL-15/IL-12 synergy induces elevated and prolonged IFN-? production by ex vivo expanded NK cells which is not due to enhanced STAT4 activation. Mol Immunol. 2017;88:138-147 pubmed publisher
Björklund A, Forkel M, Picelli S, Konya V, Theorell J, Friberg D, et al. The heterogeneity of human CD127(+) innate lymphoid cells revealed by single-cell RNA sequencing. Nat Immunol. 2016;17:451-60 pubmed publisher
Metcalf Pate K, Pohlmeyer C, Walker Sperling V, Foote J, Najarro K, Cryer C, et al. A Murine Viral Outgrowth Assay to Detect Residual HIV Type 1 in Patients With Undetectable Viral Loads. J Infect Dis. 2015;212:1387-96 pubmed publisher
Wu Z, Sinzger C, Frascaroli G, Reichel J, Bayer C, Wang L, et al. Human cytomegalovirus-induced NKG2C(hi) CD57(hi) natural killer cells are effectors dependent on humoral antiviral immunity. J Virol. 2013;87:7717-25 pubmed publisher
Vaccari M, Halwani R, Patterson L, Boasso A, Beal J, Tryniszewska E, et al. Antibodies to gp120 and PD-1 expression on virus-specific CD8+ T cells in protection from simian AIDS. J Virol. 2013;87:3526-37 pubmed publisher
Boltz A, Piater B, Toleikis L, Guenther R, Kolmar H, Hock B. Bi-specific aptamers mediating tumor cell lysis. J Biol Chem. 2011;286:21896-905 pubmed publisher
Brainard D, Seung E, Frahm N, Cariappa A, Bailey C, Hart W, et al. Induction of robust cellular and humoral virus-specific adaptive immune responses in human immunodeficiency virus-infected humanized BLT mice. J Virol. 2009;83:7305-21 pubmed publisher
Timmerman K, Flynn M, Coen P, Markofski M, Pence B. Exercise training-induced lowering of inflammatory (CD14+CD16+) monocytes: a role in the anti-inflammatory influence of exercise?. J Leukoc Biol. 2008;84:1271-8 pubmed publisher
Smed Sorensen A, Moll M, Cheng T, Lore K, Norlin A, Perbeck L, et al. IgG regulates the CD1 expression profile and lipid antigen-presenting function in human dendritic cells via FcgammaRIIa. Blood. 2008;111:5037-46 pubmed publisher
product information
Antigen :
CD16
Apps. Abbrev. :
Flow Cytometry
Cat # :
302026
Clone :
3G8
Item :
Alexa Fluor® 700 anti-human CD16
Isotype :
Mouse IgG1, κ
Other Names :
FcγRIII, Fc gamma receptor, Fc gamma receptor 3
Size :
100 μg
Price (USD) :
268 USD
Reactivity :
Human, Cynomolgus, Rhesus
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
A700
Immunogen :
Human PMN cells
Application Notes :
The 3G8 antibody clone blocks neutrophil phagocytosis and stimulates NK cell proliferation. It has been reported that this clone interacts with the Fc RIIa and Fc RIIIb receptors causing neutrophil activation and aggregation 18 . Due to this phenomenon staining in whole blood may cause a reduction in the number of granulocytes or alter their scatter profile. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections 6 , immunoprecipitation 3 , stimulation of NK cell proliferation 4 , blocking of phagocytosis 5 , and blocking of immunoglobulin binding to Fc RIII 7,8 . The Ultra-LEAF purified antibody (Endotoxin 0.01 EU/ug, Azide-Free, 0.2 um filtered) is recommended for functional assays (Cat. No. 302049, 302050, 302057, 302058).
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA