Pacific Blue™ anti-human CD16 | BioLegend 302021 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

Pacific Blue™ anti-human CD16

catalog :

302021

quantity :

100 μg

price :

268 USD

clonality :

monoclonal

host :

mouse

conjugate :

Pacific Blue

clone name :

3G8

reactivity :

human, rhesus macaque

application :

flow cytometry

more info or order :

BioLegend product webpage

citations: 15

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human	Jitschin R, Braun M, Büttner M, Dettmer Wilde K, Bricks J, Berger J, et al. CLL-cells induce IDOhi CD14+HLA-DRlo myeloid-derived suppressor cells that inhibit T-cell responses and promote TRegs. Blood. 2014;124:750-60 pubmed publisher
flow cytometry; human	Bendall S, Simonds E, Qiu P, Amir E, Krutzik P, Finck R, et al. Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum. Science. 2011;332:687-96 pubmed publisher
flow cytometry; human	Fung E, Esposito L, Todd J, Wicker L. Multiplexed immunophenotyping of human antigen-presenting cells in whole blood by polychromatic flow cytometry. Nat Protoc. 2010;5:357-70 pubmed publisher
	Ilinykh P, Huang K, Santos R, Gilchuk P, Gunn B, Karim M, et al. Non-neutralizing Antibodies from a Marburg Infection Survivor Mediate Protection by Fc-Effector Functions and by Enhancing Efficacy of Other Antibodies. Cell Host Microbe. 2020;27:976-991.e11 pubmed publisher
	Zysset D, Weber B, Rihs S, Brasseit J, Freigang S, Riether C, et al. TREM-1 links dyslipidemia to inflammation and lipid deposition in atherosclerosis. Nat Commun. 2016;7:13151 pubmed publisher
	DeGottardi M, Okoye A, Vaidya M, Talla A, Konfe A, Reyes M, et al. Effect of Anti-IL-15 Administration on T Cell and NK Cell Homeostasis in Rhesus Macaques. J Immunol. 2016;197:1183-98 pubmed publisher
	Shirai T, Nazarewicz R, Wallis B, Yanes R, Watanabe R, Hilhorst M, et al. The glycolytic enzyme PKM2 bridges metabolic and inflammatory dysfunction in coronary artery disease. J Exp Med. 2016;213:337-54 pubmed publisher
	Harwood N, Golden Mason L, Cheng L, Rosen H, Mengshol J. HCV-infected cells and differentiation increase monocyte immunoregulatory galectin-9 production. J Leukoc Biol. 2016;99:495-503 pubmed publisher
	Mandl M, Schmitz S, Weber C, Hristov M. Characterization of the CD14++CD16+ monocyte population in human bone marrow. PLoS ONE. 2014;9:e112140 pubmed publisher
	Wu Z, Sinzger C, Frascaroli G, Reichel J, Bayer C, Wang L, et al. Human cytomegalovirus-induced NKG2C(hi) CD57(hi) natural killer cells are effectors dependent on humoral antiviral immunity. J Virol. 2013;87:7717-25 pubmed publisher
	Boltz A, Piater B, Toleikis L, Guenther R, Kolmar H, Hock B. Bi-specific aptamers mediating tumor cell lysis. J Biol Chem. 2011;286:21896-905 pubmed publisher
	Dessouki O, Kamiya Y, Nagahama H, Tanaka M, Suzu S, Sasaki Y, et al. Chronic hepatitis C viral infection reduces NK cell frequency and suppresses cytokine secretion: Reversion by anti-viral treatment. Biochem Biophys Res Commun. 2010;393:331-7 pubmed publisher
	Brainard D, Seung E, Frahm N, Cariappa A, Bailey C, Hart W, et al. Induction of robust cellular and humoral virus-specific adaptive immune responses in human immunodeficiency virus-infected humanized BLT mice. J Virol. 2009;83:7305-21 pubmed publisher
	Timmerman K, Flynn M, Coen P, Markofski M, Pence B. Exercise training-induced lowering of inflammatory (CD14+CD16+) monocytes: a role in the anti-inflammatory influence of exercise?. J Leukoc Biol. 2008;84:1271-8 pubmed publisher
	Smed Sorensen A, Moll M, Cheng T, Lore K, Norlin A, Perbeck L, et al. IgG regulates the CD1 expression profile and lipid antigen-presenting function in human dendritic cells via FcgammaRIIa. Blood. 2008;111:5037-46 pubmed publisher

product information

Antigen :

CD16

Apps. Abbrev. :

Flow Cytometry

Cat # :

302021

Clone :

3G8

Item :

Pacific Blue™ anti-human CD16

Isotype :

Mouse IgG1, κ

Other Names :

FcγRIII, Fc gamma receptor, Fc gamma receptor 3

Size :

100 μg

Price (USD) :

268 USD

Reactivity :

Human, Cynomolgus, Rhesus

Clonality :

Monoclonal

Host :

Mouse

Conjugate/Tag/Label :

Immunogen :

Human PMN cells

Application Notes :

The 3G8 antibody clone blocks neutrophil phagocytosis and stimulates NK cell proliferation. It has been reported that this clone interacts with the Fc RIIa and Fc RIIIb receptors causing neutrophil activation and aggregation 18 . Due to this phenomenon staining in whole blood may cause a reduction in the number of granulocytes or alter their scatter profile. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections 6 , immunoprecipitation 3 , stimulation of NK cell proliferation 4 , blocking of phagocytosis 5 , and blocking of immunoglobulin binding to Fc RIII 7,8 . The Ultra-LEAF purified antibody (Endotoxin 0.01 EU/ug, Azide-Free, 0.2 um filtered) is recommended for functional assays (Cat. No. 302049, 302050, 302057, 302058).

more info or order :

BioLegend product webpage