product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
APC/Cyanine7 anti-human CD16
catalog :
302018
quantity :
100 tests
price :
316 USD
clonality :
monoclonal
host :
mouse
conjugate :
APC/Cy7
clone name :
3G8
reactivity :
human, rhesus macaque
application :
flow cytometry
more info or order :
citations: 38
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; fig 4
Weldon A, Moldovan I, Cabling M, Hernandez E, Hsu S, Gonzalez J, et al. Surface APRIL Is Elevated on Myeloid Cells and Is Associated with Disease Activity in Patients with Rheumatoid Arthritis. J Rheumatol. 2015;42:749-59 pubmed publisher
  • flow cytometry; human
Bendall S, Simonds E, Qiu P, Amir E, Krutzik P, Finck R, et al. Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum. Science. 2011;332:687-96 pubmed publisher
Ehlers L, Kirchner M, Mertins P, Strehl C, Buttgereit F, Gaber T. The Anti-Glucocorticoid Receptor Antibody Clone 5E4: Raising Awareness of Unspecific Antibody Binding. Int J Mol Sci. 2022;23: pubmed publisher
Richert Spuhler L, MAR C, Shinde P, Wu F, Hong T, Greene E, et al. CD101 genetic variants modify regulatory and conventional T cell phenotypes and functions. Cell Rep Med. 2021;2:100322 pubmed publisher
Ehlers L, Kuppe A, Damerau A, Wilantri S, Kirchner M, Mertins P, et al. Surface AMP deaminase 2 as a novel regulator modifying extracellular adenine nucleotide metabolism. FASEB J. 2021;35:e21684 pubmed publisher
Herati R, Silva L, Vella L, Muselman A, Alanio C, Bengsch B, et al. Vaccine-induced ICOS+CD38+ circulating Tfh are sensitive biosensors of age-related changes in inflammatory pathways. Cell Rep Med. 2021;2:100262 pubmed publisher
Caduff N, McHugh D, Rieble L, Forconi C, Ong echa J, Oluoch P, et al. KSHV infection drives poorly cytotoxic CD56-negative natural killer cell differentiation in vivo upon KSHV/EBV dual infection. Cell Rep. 2021;35:109056 pubmed publisher
Ponath V, Hoffmann N, Bergmann L, Mäder C, Alashkar Alhamwe B, Preußer C, et al. Secreted Ligands of the NK Cell Receptor NKp30: B7-H6 Is in Contrast to BAG6 Only Marginally Released via Extracellular Vesicles. Int J Mol Sci. 2021;22: pubmed publisher
Holokai L, Chakrabarti J, Lundy J, Croagh D, Adhikary P, Richards S, et al. Murine- and Human-Derived Autologous Organoid/Immune Cell Co-Cultures as Pre-Clinical Models of Pancreatic Ductal Adenocarcinoma. Cancers (Basel). 2020;12: pubmed publisher
Corrado M, Edwards Hicks J, Villa M, Flachsmann L, Sanin D, Jacobs M, et al. Dynamic Cardiolipin Synthesis Is Required for CD8+ T Cell Immunity. Cell Metab. 2020;32:981-995.e7 pubmed publisher
Sharma A, Seow J, Dutertre C, Pai R, Blériot C, Mishra A, et al. Onco-fetal Reprogramming of Endothelial Cells Drives Immunosuppressive Macrophages in Hepatocellular Carcinoma. Cell. 2020;183:377-394.e21 pubmed publisher
Bäcker Koduah P, Infante Duarte C, Ivaldi F, Uccelli A, Bellmann Strobl J, Wernecke K, et al. Effect of vitamin D supplementation on N-glycan branching and cellular immunophenotypes in MS. Ann Clin Transl Neurol. 2020;: pubmed publisher
Wiedemann A, Foucat E, Hocini H, Lefebvre C, Hejblum B, Durand M, et al. Long-lasting severe immune dysfunction in Ebola virus disease survivors. Nat Commun. 2020;11:3730 pubmed publisher
Flak M, Koenis D, Sobrino A, Smith J, Pistorius K, Palmas F, et al. GPR101 mediates the pro-resolving actions of RvD5n-3 DPA in arthritis and infections. J Clin Invest. 2020;130:359-373 pubmed publisher
Dutertre C, Becht E, Irac S, Khalilnezhad A, Narang V, Khalilnezhad S, et al. Single-Cell Analysis of Human Mononuclear Phagocytes Reveals Subset-Defining Markers and Identifies Circulating Inflammatory Dendritic Cells. Immunity. 2019;51:573-589.e8 pubmed publisher
Ong S, Teng K, Newell E, Chen H, Chen J, Loy T, et al. A Novel, Five-Marker Alternative to CD16-CD14 Gating to Identify the Three Human Monocyte Subsets. Front Immunol. 2019;10:1761 pubmed publisher
Hahn J, Euler M, Kilgus E, Kienhöfer D, Stoof J, Knopf J, et al. NOX2 mediates quiescent handling of dead cell remnants in phagocytes. Redox Biol. 2019;26:101279 pubmed publisher
Cirelli K, Carnathan D, Nogal B, Martin J, Rodriguez O, Upadhyay A, et al. Slow Delivery Immunization Enhances HIV Neutralizing Antibody and Germinal Center Responses via Modulation of Immunodominance. Cell. 2019;177:1153-1171.e28 pubmed publisher
Foote J, Patel A, Yona S, Segal A. Variations in the Phagosomal Environment of Human Neutrophils and Mononuclear Phagocyte Subsets. Front Immunol. 2019;10:188 pubmed publisher
Friedensohn S, Lindner J, Cornacchione V, Iazeolla M, Miho E, Zingg A, et al. Synthetic Standards Combined With Error and Bias Correction Improve the Accuracy and Quantitative Resolution of Antibody Repertoire Sequencing in Human Naïve and Memory B Cells. Front Immunol. 2018;9:1401 pubmed publisher
Frasca D, Diaz A, Romero M, Thaller S, Blomberg B. Secretion of autoimmune antibodies in the human subcutaneous adipose tissue. PLoS ONE. 2018;13:e0197472 pubmed publisher
Perry J, Russler Germain E, Zhou Y, Purtha W, Cooper M, Choi J, et al. Transfer of Cell-Surface Antigens by Scavenger Receptor CD36 Promotes Thymic Regulatory T Cell Receptor Repertoire Development and Allo-tolerance. Immunity. 2018;48:923-936.e4 pubmed publisher
Felices M, Lenvik A, McElmurry R, Chu S, Hinderlie P, Bendzick L, et al. Continuous treatment with IL-15 exhausts human NK cells via a metabolic defect. JCI Insight. 2018;3: pubmed publisher
Hydes T, Noll A, Salinas Riester G, Abuhilal M, Armstrong T, Hamady Z, et al. IL-12 and IL-15 induce the expression of CXCR6 and CD49a on peripheral natural killer cells. Immun Inflamm Dis. 2018;6:34-46 pubmed publisher
Dobbs K, Embury P, Vulule J, Odada P, Rosa B, Mitreva M, et al. Monocyte dysregulation and systemic inflammation during pediatric falciparum malaria. JCI Insight. 2017;2: pubmed publisher
McCracken M, Gromowski G, Friberg H, Lin X, Abbink P, De La Barrera R, et al. Impact of prior flavivirus immunity on Zika virus infection in rhesus macaques. PLoS Pathog. 2017;13:e1006487 pubmed publisher
Dai H, Griffin N, Bolyard C, Mao H, Zhang J, Cripe T, et al. The Fc Domain of Immunoglobulin Is Sufficient to Bridge NK Cells with Virally Infected Cells. Immunity. 2017;47:159-170.e10 pubmed publisher
Woodberry T, Loughland J, Minigo G, Burel J, Amante F, Piera K, et al. Early Immune Regulatory Changes in a Primary Controlled Human Plasmodium vivax Infection: CD1c+ Myeloid Dendritic Cell Maturation Arrest, Induction of the Kynurenine Pathway, and Regulatory T Cell Activation. Infect Immun. 2017;85: pubmed publisher
Vallera D, Felices M, McElmurry R, McCullar V, Zhou X, Schmohl J, et al. IL15 Trispecific Killer Engagers (TriKE) Make Natural Killer Cells Specific to CD33+ Targets While Also Inducing Persistence, In Vivo Expansion, and Enhanced Function. Clin Cancer Res. 2016;22:3440-50 pubmed publisher
Romani B, Baygloo N, Hamidi Fard M, Aghasadeghi M, Allahbakhshi E. HIV-1 Vpr Protein Induces Proteasomal Degradation of Chromatin-associated Class I HDACs to Overcome Latent Infection of Macrophages. J Biol Chem. 2016;291:2696-711 pubmed publisher
Bratcher P, Rowe S, Reeves G, Roberts T, Szul T, Harris W, et al. Alterations in blood leukocytes of G551D-bearing cystic fibrosis patients undergoing treatment with ivacaftor. J Cyst Fibros. 2016;15:67-73 pubmed publisher
Gleason M, Ross J, Warlick E, Lund T, Verneris M, Wiernik A, et al. CD16xCD33 bispecific killer cell engager (BiKE) activates NK cells against primary MDS and MDSC CD33+ targets. Blood. 2014;123:3016-26 pubmed publisher
Wiernik A, Foley B, Zhang B, Verneris M, Warlick E, Gleason M, et al. Targeting natural killer cells to acute myeloid leukemia in vitro with a CD16 x 33 bispecific killer cell engager and ADAM17 inhibition. Clin Cancer Res. 2013;19:3844-55 pubmed publisher
Wu Z, Sinzger C, Frascaroli G, Reichel J, Bayer C, Wang L, et al. Human cytomegalovirus-induced NKG2C(hi) CD57(hi) natural killer cells are effectors dependent on humoral antiviral immunity. J Virol. 2013;87:7717-25 pubmed publisher
Boltz A, Piater B, Toleikis L, Guenther R, Kolmar H, Hock B. Bi-specific aptamers mediating tumor cell lysis. J Biol Chem. 2011;286:21896-905 pubmed publisher
Brainard D, Seung E, Frahm N, Cariappa A, Bailey C, Hart W, et al. Induction of robust cellular and humoral virus-specific adaptive immune responses in human immunodeficiency virus-infected humanized BLT mice. J Virol. 2009;83:7305-21 pubmed publisher
Timmerman K, Flynn M, Coen P, Markofski M, Pence B. Exercise training-induced lowering of inflammatory (CD14+CD16+) monocytes: a role in the anti-inflammatory influence of exercise?. J Leukoc Biol. 2008;84:1271-8 pubmed publisher
Smed Sorensen A, Moll M, Cheng T, Lore K, Norlin A, Perbeck L, et al. IgG regulates the CD1 expression profile and lipid antigen-presenting function in human dendritic cells via FcgammaRIIa. Blood. 2008;111:5037-46 pubmed publisher
product information
Antigen :
CD16
Apps. Abbrev. :
Flow Cytometry
Cat # :
302018
Clone :
3G8
Item :
APC/Cyanine7 anti-human CD16
Isotype :
Mouse IgG1, κ
Other Names :
FcγRIII, Fc gamma receptor, Fc gamma receptor 3
Size :
100 tests
Price (USD) :
316 USD
Reactivity :
Human, Cynomolgus, Rhesus
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
APC_CYANINE7
Immunogen :
Human PMN cells
Application Notes :
The 3G8 antibody clone blocks neutrophil phagocytosis and stimulates NK cell proliferation. It has been reported that this clone interacts with the Fc RIIa and Fc RIIIb receptors causing neutrophil activation and aggregation 18 . Due to this phenomenon staining in whole blood may cause a reduction in the number of granulocytes or alter their scatter profile. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections 6 , immunoprecipitation 3 , stimulation of NK cell proliferation 4 , blocking of phagocytosis 5 , and blocking of immunoglobulin binding to Fc RIII 7,8 . The Ultra-LEAF purified antibody (Endotoxin 0.01 EU/ug, Azide-Free, 0.2 um filtered) is recommended for functional assays (Cat. No. 302049, 302050, 302057, 302058).
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA