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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
PE/Cyanine7 anti-human CD16
catalog :
302015
quantity :
25 tests
price :
143 USD
clonality :
monoclonal
host :
mouse
conjugate :
PE/Cy7
clone name :
3G8
reactivity :
human, rhesus macaque
application :
flow cytometry
more info or order :
citations: 34
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; fig 7
Venkatasubramanian S, Tripathi D, Tucker T, Paidipally P, Cheekatla S, Welch E, et al. Tissue factor expression by myeloid cells contributes to protective immune response against Mycobacterium tuberculosis infection. Eur J Immunol. 2016;46:464-79 pubmed publisher
  • flow cytometry; human
Mohanty S, Joshi S, Ueda I, Wilson J, Blevins T, Siconolfi B, et al. Prolonged proinflammatory cytokine production in monocytes modulated by interleukin 10 after influenza vaccination in older adults. J Infect Dis. 2015;211:1174-84 pubmed publisher
  • flow cytometry; human
Jiang B, Wu X, Li X, Yang X, Zhou Y, Yan H, et al. Expansion of NK cells by engineered K562 cells co-expressing 4-1BBL and mMICA, combined with soluble IL-21. Cell Immunol. 2014;290:10-20 pubmed publisher
  • flow cytometry; human
Wilson E, Singh A, Hullsiek K, Gibson D, Henry W, Lichtenstein K, et al. Monocyte-activation phenotypes are associated with biomarkers of inflammation and coagulation in chronic HIV infection. J Infect Dis. 2014;210:1396-406 pubmed publisher
  • flow cytometry; human
Sereti I, Estes J, Thompson W, Morcock D, Fischl M, Croughs T, et al. Decreases in colonic and systemic inflammation in chronic HIV infection after IL-7 administration. PLoS Pathog. 2014;10:e1003890 pubmed publisher
  • flow cytometry; human
Bendall S, Simonds E, Qiu P, Amir E, Krutzik P, Finck R, et al. Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum. Science. 2011;332:687-96 pubmed publisher
Mol S, Hafkamp F, Varela L, Simkhada N, Taanman Kueter E, Tas S, et al. Efficient Neutrophil Activation Requires Two Simultaneous Activating Stimuli. Int J Mol Sci. 2021;22: pubmed publisher
Abdul Jawad S, Baù L, Alaguthurai T, Del Molino Del Barrio I, Laing A, Hayday T, et al. Acute Immune Signatures and Their Legacies in Severe Acute Respiratory Syndrome Coronavirus-2 Infected Cancer Patients. Cancer Cell. 2021;39:257-275.e6 pubmed publisher
Golebski K, Layhadi J, Sahiner U, Steveling Klein E, Lenormand M, Li R, et al. Induction of IL-10-producing type 2 innate lymphoid cells by allergen immunotherapy is associated with clinical response. Immunity. 2021;54:291-307.e7 pubmed publisher
Nafria M, Bonifer C, Stanley E, Ng E, Elefanty A. Protocol for the Generation of Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells. STAR Protoc. 2020;1:100130 pubmed publisher
Evans R, Antonelou M, Sathiananthamoorthy S, Rega M, Henderson S, Ceron Gutierrez L, et al. Inherited salt-losing tubulopathies are associated with immunodeficiency due to impaired IL-17 responses. Nat Commun. 2020;11:4368 pubmed publisher
Laing A, Lorenc A, Del Molino Del Barrio I, Das A, Fish M, Monin L, et al. A dynamic COVID-19 immune signature includes associations with poor prognosis. Nat Med. 2020;26:1623-1635 pubmed publisher
Aguilar Briseño J, Upasani V, Ellen B, Moser J, Pauzuolis M, Ruiz Silva M, et al. TLR2 on blood monocytes senses dengue virus infection and its expression correlates with disease pathogenesis. Nat Commun. 2020;11:3177 pubmed publisher
Cirovic B, de Bree L, Groh L, Blok B, Chan J, van der Velden W, et al. BCG Vaccination in Humans Elicits Trained Immunity via the Hematopoietic Progenitor Compartment. Cell Host Microbe. 2020;28:322-334.e5 pubmed publisher
Nafria M, Keane P, Ng E, Stanley E, Elefanty A, Bonifer C. Expression of RUNX1-ETO Rapidly Alters the Chromatin Landscape and Growth of Early Human Myeloid Precursor Cells. Cell Rep. 2020;31:107691 pubmed publisher
Riquelme S, Liimatta K, Wong Fok Lung T, Fields B, Ahn D, Chen D, et al. Pseudomonas aeruginosa Utilizes Host-Derived Itaconate to Redirect Its Metabolism to Promote Biofilm Formation. Cell Metab. 2020;31:1091-1106.e6 pubmed publisher
Jackson Jones L, Smith P, Portman J, Magalhaes M, Mylonas K, Vermeren M, et al. Stromal Cells Covering Omental Fat-Associated Lymphoid Clusters Trigger Formation of Neutrophil Aggregates to Capture Peritoneal Contaminants. Immunity. 2020;52:700-715.e6 pubmed publisher
Serra E, Schwerd T, Moutsianas L, Cavounidis A, Fachal L, Pandey S, et al. Somatic mosaicism and common genetic variation contribute to the risk of very-early-onset inflammatory bowel disease. Nat Commun. 2020;11:995 pubmed publisher
Escudero Pérez B, Ruibal P, Rottstegge M, Lüdtke A, Port J, Hartmann K, et al. Comparative pathogenesis of Ebola virus and Reston virus infection in humanized mice. JCI Insight. 2019;4: pubmed publisher
Dos Santos J, Barroso de Figueiredo A, Teodoro Silva M, Cirovic B, de Bree L, Damen M, et al. β-Glucan-Induced Trained Immunity Protects against Leishmania braziliensis Infection: a Crucial Role for IL-32. Cell Rep. 2019;28:2659-2672.e6 pubmed publisher
Walk J, de Bree L, Graumans W, Stoter R, van Gemert G, van de Vegte Bolmer M, et al. Outcomes of controlled human malaria infection after BCG vaccination. Nat Commun. 2019;10:874 pubmed publisher
He W, Holtkamp S, Hergenhan S, Kraus K, De Juan A, Weber J, et al. Circadian Expression of Migratory Factors Establishes Lineage-Specific Signatures that Guide the Homing of Leukocyte Subsets to Tissues. Immunity. 2018;49:1175-1190.e7 pubmed publisher
Kong X, Martinez Barricarte R, Kennedy J, Mele F, Lazarov T, Deenick E, et al. Disruption of an antimycobacterial circuit between dendritic and helper T cells in human SPPL2a deficiency. Nat Immunol. 2018;19:973-985 pubmed publisher
Loelius S, Spinelli S, Lannan K, Phipps R. In Vitro Methods to Characterize the Effects of Tobacco and Nontobacco Products on Human Platelet Function. Curr Protoc Toxicol. 2018;76:e46 pubmed publisher
Hunter S, Willcox C, Davey M, Kasatskaya S, Jeffery H, Chudakov D, et al. Human liver infiltrating γδ T cells are composed of clonally expanded circulating and tissue-resident populations. J Hepatol. 2018;69:654-665 pubmed publisher
Lee J, Tam H, Adler L, Ilstad Minnihan A, Macaubas C, Mellins E. The MHC class II antigen presentation pathway in human monocytes differs by subset and is regulated by cytokines. PLoS ONE. 2017;12:e0183594 pubmed publisher
Demers K, Makedonas G, Buggert M, Eller M, Ratcliffe S, Goonetilleke N, et al. Temporal Dynamics of CD8+ T Cell Effector Responses during Primary HIV Infection. PLoS Pathog. 2016;12:e1005805 pubmed publisher
Llewellyn D, Miura K, Fay M, Williams A, Murungi L, Shi J, et al. Standardization of the antibody-dependent respiratory burst assay with human neutrophils and Plasmodium falciparum malaria. Sci Rep. 2015;5:14081 pubmed publisher
Collison J, Carlin L, Eichmann M, Geissmann F, Peakman M. Heterogeneity in the Locomotory Behavior of Human Monocyte Subsets over Human Vascular Endothelium In Vitro. J Immunol. 2015;195:1162-70 pubmed publisher
Wu Z, Sinzger C, Frascaroli G, Reichel J, Bayer C, Wang L, et al. Human cytomegalovirus-induced NKG2C(hi) CD57(hi) natural killer cells are effectors dependent on humoral antiviral immunity. J Virol. 2013;87:7717-25 pubmed publisher
Boltz A, Piater B, Toleikis L, Guenther R, Kolmar H, Hock B. Bi-specific aptamers mediating tumor cell lysis. J Biol Chem. 2011;286:21896-905 pubmed publisher
Brainard D, Seung E, Frahm N, Cariappa A, Bailey C, Hart W, et al. Induction of robust cellular and humoral virus-specific adaptive immune responses in human immunodeficiency virus-infected humanized BLT mice. J Virol. 2009;83:7305-21 pubmed publisher
Timmerman K, Flynn M, Coen P, Markofski M, Pence B. Exercise training-induced lowering of inflammatory (CD14+CD16+) monocytes: a role in the anti-inflammatory influence of exercise?. J Leukoc Biol. 2008;84:1271-8 pubmed publisher
Smed Sorensen A, Moll M, Cheng T, Lore K, Norlin A, Perbeck L, et al. IgG regulates the CD1 expression profile and lipid antigen-presenting function in human dendritic cells via FcgammaRIIa. Blood. 2008;111:5037-46 pubmed publisher
product information
Antigen :
CD16
Apps. Abbrev. :
Flow Cytometry
Cat # :
302015
Clone :
3G8
Item :
PE/Cyanine7 anti-human CD16
Isotype :
Mouse IgG1, κ
Other Names :
FcγRIII, Fc gamma receptor, Fc gamma receptor 3
Size :
25 tests
Price (USD) :
143 USD
Reactivity :
Human, Cynomolgus, Rhesus
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
PE_CYANINE7
Immunogen :
Human PMN cells
Application Notes :
The 3G8 antibody clone blocks neutrophil phagocytosis and stimulates NK cell proliferation. It has been reported that this clone interacts with the Fc RIIa and Fc RIIIb receptors causing neutrophil activation and aggregation 18 . Due to this phenomenon staining in whole blood may cause a reduction in the number of granulocytes or alter their scatter profile. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections 6 , immunoprecipitation 3 , stimulation of NK cell proliferation 4 , blocking of phagocytosis 5 , and blocking of immunoglobulin binding to Fc RIII 7,8 . The Ultra-LEAF purified antibody (Endotoxin 0.01 EU/ug, Azide-Free, 0.2 um filtered) is recommended for functional assays (Cat. No. 302049, 302050, 302057, 302058).
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA