LEAF™ Purified anti-human CD16 | BioLegend 302014 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

LEAF™ Purified anti-human CD16

catalog :

302014

quantity :

500 μg

price :

350 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

3G8

reactivity :

human, rhesus macaque

application :

neutralization, immunoprecipitation, flow cytometry, immunohistochemistry - frozen section

citations: 10

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; loading ...; fig 4b	Muhammad F, Wang D, Montieth A, Lee S, Preble J, Foster C, et al. PD-1+ melanocortin receptor dependent-Treg cells prevent autoimmune disease. Sci Rep. 2019;9:16941 pubmed publisher
flow cytometry; human; fig 1	O Brien E, Abdulahad W, Rutgers A, Huitema M, O Reilly V, Coughlan A, et al. Intermediate monocytes in ANCA vasculitis: increased surface expression of ANCA autoantigens and IL-1Î² secretion in response to anti-MPO antibodies. Sci Rep. 2015;5:11888 pubmed publisher
flow cytometry; human; fig 1	HÃ¼nniger K, Bieber K, Martin R, Lehnert T, Figge M, LÃ¶ffler J, et al. A second stimulus required for enhanced antifungal activity of human neutrophils in blood is provided by anaphylatoxin C5a. J Immunol. 2015;194:1199-210 pubmed publisher
flow cytometry; human	Bendall S, Simonds E, Qiu P, Amir E, Krutzik P, Finck R, et al. Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum. Science. 2011;332:687-96 pubmed publisher
	Gruber C, Calis J, Buta S, Evrony G, Martin J, Uhl S, et al. Complex Autoinflammatory Syndrome Unveils Fundamental Principles of JAK1 Kinase Transcriptional and Biochemical Function. Immunity. 2020;53:672-684.e11 pubmed publisher
	Martin J, Chang C, Boschetti G, Ungaro R, Giri M, Grout J, et al. Single-Cell Analysis of Crohn's Disease Lesions Identifies a Pathogenic Cellular Module Associated with Resistance to Anti-TNF Therapy. Cell. 2019;178:1493-1508.e20 pubmed publisher
	Vidard L, Dureuil C, Baudhuin J, Vescovi L, Durand L, Sierra V, et al. CD137 (4-1BB) Engagement Fine-Tunes Synergistic IL-15- and IL-21-Driven NK Cell Proliferation. J Immunol. 2019;: pubmed publisher
	Rölle A, Meyer M, Calderazzo S, Jager D, Momburg F. Distinct HLA-E Peptide Complexes Modify Antibody-Driven Effector Functions of Adaptive NK Cells. Cell Rep. 2018;24:1967-1976.e4 pubmed publisher
	van der Heijden J, Breunis W, Geissler J, de Boer M, van den Berg T, Kuijpers T. Phenotypic variation in IgG receptors by nonclassical FCGR2C alleles. J Immunol. 2012;188:1318-24 pubmed publisher
	Goodridge J, Lathbury L, John E, Charles A, Christiansen F, Witt C. The genotype of the NK cell receptor, KIR2DL4, influences INFgamma secretion by decidual natural killer cells. Mol Hum Reprod. 2009;15:489-97 pubmed publisher

product information

Antigen :

CD16

Apps. Abbrev. :

Flow Cytometry, CyTOF®, Block, IHC-F, IP, Stim

Cat # :

302014

Clone :

3G8

Item :

LEAF™ Purified anti-human CD16

Isotype :

Mouse IgG1, κ

Other Names :

FcγRIII

Size :

500 μg

Price (USD) :

350 USD

Reactivity :

Human, African Green, Baboon, Capuchin Monkey, Chimpanzee, Cynomolgus, Marmoset, Pigtailed Macaque, Rhesus, Sooty Mangabey, Squirrel Monkey

Clonality :

Monoclonal

Host :

Mouse

Conjugate/Tag/Label :

LEAF

Immunogen :

Human PMN cells

Application Notes :

The 3G8 antibody clone blocks neutrophil phagocytosis and stimulates NK cell proliferation. It has been reported that this clone interacts with the Fc?RIIa and Fc?RIIIb receptors causing neutrophil activation and aggregation 18 . Due to this phenomenon staining in whole blood may cause a reduction in the number of granulocytes or alter their scatter profile. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections 6 , immunoprecipitation 3 , stimulation of NK cell proliferation 4 , blocking of phagocytosis 5 , and blocking of immunoglobulin binding to Fc?RIII 7,8 . The LEAF purified antibody (Endotoxin 0.1 EU/ug, Azide-Free, 0.2 um filtered) is recommended for functional assays (Cat. No. 302014). For highly sensitive assays, we recommend Ultra-LEAF purified antibody (Cat. No. 302050) with a lower endotoxin limit than standard LEAF purified antibodies (Endotoxin 0.01 EU/ug).