product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
APC anti-human CD16
catalog :
302011
quantity :
25 tests
price :
132 USD
clonality :
monoclonal
host :
mouse
conjugate :
APC
clone name :
3G8
reactivity :
human, rhesus macaque
application :
flow cytometry
more info or order :
citations: 31
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; loading ...; fig 6
Xia Y, Gao Y, Wang B, Zhang H, Zhang Q. Optimizing the Method of Cell Separation from Bile of Patients with Cholangiocarcinoma for Flow Cytometry. Gastroenterol Res Pract. 2019;2019:5436961 pubmed publisher
  • flow cytometry; human; fig 1
O Brien E, Abdulahad W, Rutgers A, Huitema M, O Reilly V, Coughlan A, et al. Intermediate monocytes in ANCA vasculitis: increased surface expression of ANCA autoantigens and IL-1β secretion in response to anti-MPO antibodies. Sci Rep. 2015;5:11888 pubmed publisher
  • flow cytometry; human; fig 1
Hünniger K, Bieber K, Martin R, Lehnert T, Figge M, Löffler J, et al. A second stimulus required for enhanced antifungal activity of human neutrophils in blood is provided by anaphylatoxin C5a. J Immunol. 2015;194:1199-210 pubmed publisher
  • flow cytometry; human
Bendall S, Simonds E, Qiu P, Amir E, Krutzik P, Finck R, et al. Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum. Science. 2011;332:687-96 pubmed publisher
Beatson R, Parente Pereira A, Halim L, Cozzetto D, Hull C, Whilding L, et al. TGF-β1 potentiates Vγ9Vδ2 T cell adoptive immunotherapy of cancer. Cell Rep Med. 2021;2:100473 pubmed publisher
Hiatt J, Cavero D, McGregor M, Zheng W, Budzik J, Roth T, et al. Efficient generation of isogenic primary human myeloid cells using CRISPR-Cas9 ribonucleoproteins. Cell Rep. 2021;35:109105 pubmed publisher
Ruth J, Gurrea Rubio M, Athukorala K, Rasmussen S, Weber D, Randon P, et al. CD6 is a target for cancer immunotherapy. JCI Insight. 2021;6: pubmed publisher
González Tajuelo R, González Sánchez E, Silván J, Muñoz Callejas A, Vicente Rabaneda E, Garcia Perez J, et al. Relevance of PSGL-1 Expression in B Cell Development and Activation. Front Immunol. 2020;11:588212 pubmed publisher
Tamminga M, Oomens L, Hiltermann T, Andree K, Tibbe A, Broekmaat J, et al. Microsieves for the detection of circulating tumor cells in leukapheresis product in non-small cell lung cancer patients. Transl Lung Cancer Res. 2020;9:1093-1100 pubmed publisher
Wang H, Yang J, Pan H, Tai M, Maher M, Jia R, et al. Dinutuximab Synergistically Enhances the Cytotoxicity of Natural Killer Cells to Retinoblastoma Through the Perforin-Granzyme B Pathway. Onco Targets Ther. 2020;13:3903-3920 pubmed publisher
Gopinath A, Doty A, Mackie P, Hashimi B, Francis M, Saadatpour L, et al. A novel approach to study markers of dopamine signaling in peripheral immune cells. J Immunol Methods. 2020;476:112686 pubmed publisher
Hagan T, Cortese M, Rouphael N, Boudreau C, Linde C, Maddur M, et al. Antibiotics-Driven Gut Microbiome Perturbation Alters Immunity to Vaccines in Humans. Cell. 2019;178:1313-1328.e13 pubmed publisher
Pazina T, James A, Colby K, Yang Y, GALE A, Jhatakia A, et al. Enhanced SLAMF7 Homotypic Interactions by Elotuzumab Improves NK Cell Killing of Multiple Myeloma. Cancer Immunol Res. 2019;7:1633-1646 pubmed publisher
Rizzo M, Crawford R, Bach A, Sermet S, Amalfitano A, Kaminski N. Δ9-Tetrahydrocannabinol Suppresses Monocyte-Mediated Astrocyte Production of Monocyte Chemoattractant Protein 1 and Interleukin-6 in a Toll-Like Receptor 7-Stimulated Human Coculture. J Pharmacol Exp Ther. 2019;371:191-201 pubmed publisher
Rizzo M, Crawford R, Bach A, Sermet S, Amalfitano A, Kaminski N. Imiquimod and interferon-alpha augment monocyte-mediated astrocyte secretion of MCP-1, IL-6 and IP-10 in a human co-culture system. J Neuroimmunol. 2019;333:576969 pubmed publisher
Rydbirk R, Elfving B, Folke J, Pakkenberg B, Winge K, Brudek T, et al. Increased prefrontal cortex interleukin-2 protein levels and shift in the peripheral T cell population in progressive supranuclear palsy patients. Sci Rep. 2019;9:7781 pubmed publisher
Guo L, Chen G, Zhang W, Zhou L, Xiao T, Di X, et al. A high-risk luminal A dominant breast cancer subtype with increased mobility. Breast Cancer Res Treat. 2019;175:459-472 pubmed publisher
Hilt Z, Pariser D, Ture S, Mohan A, Quijada P, Asante A, et al. Platelet-derived β2M regulates monocyte inflammatory responses. JCI Insight. 2019;: pubmed publisher
Yabe I, Truitt L, Espinoza D, Wu C, Koelle S, Panch S, et al. Barcoding of Macaque Hematopoietic Stem and Progenitor Cells: A Robust Platform to Assess Vector Genotoxicity. Mol Ther Methods Clin Dev. 2018;11:143-154 pubmed publisher
Tang Huau T, Guéguen P, Goudot C, Durand M, Bohec M, Baulande S, et al. Human in vivo-generated monocyte-derived dendritic cells and macrophages cross-present antigens through a vacuolar pathway. Nat Commun. 2018;9:2570 pubmed publisher
Zhai Y, Lin P, Feng Z, Lu H, Han Q, Chen J, et al. TNFAIP3-DEPTOR complex regulates inflammasome secretion through autophagy in ankylosing spondylitis monocytes. Autophagy. 2018;14:1629-1643 pubmed publisher
Kim M, Yu K, Kenderian S, Ruella M, Chen S, Shin T, et al. Genetic Inactivation of CD33 in Hematopoietic Stem Cells to Enable CAR T Cell Immunotherapy for Acute Myeloid Leukemia. Cell. 2018;173:1439-1453.e19 pubmed publisher
Zhong J, Scholz T, Yau A, Guérard S, Huffmeier U, Burkhardt H, et al. Mannan-induced Nos2 in macrophages enhances IL-17-driven psoriatic arthritis by innate lymphocytes. Sci Adv. 2018;4:eaas9864 pubmed publisher
Motwani M, Colas R, George M, Flint J, Dalli J, Richard Loendt A, et al. Pro-resolving mediators promote resolution in a human skin model of UV-killed Escherichia coli-driven acute inflammation. JCI Insight. 2018;3: pubmed publisher
Rizzo M, Crawford R, Henriquez J, ALDHAMEN Y, Gulick P, Amalfitano A, et al. HIV-infected cannabis users have lower circulating CD16+ monocytes and IP-10 levels compared to non-using HIV patients. AIDS. 2017;: pubmed publisher
Kho D, Johnson R, Robilliard L, du Mez E, McIntosh J, O Carroll S, et al. ECIS technology reveals that monocytes isolated by CD14+ve selection mediate greater loss of BBB integrity than untouched monocytes, which occurs to a greater extent with IL-1β activated endothelium in comparison to TNFα. PLoS ONE. 2017;12:e0180267 pubmed publisher
Chen J, Cai Z, Zhang L, Yin Y, Chen X, Chen C, et al. Lis1 Regulates Germinal Center B Cell Antigen Acquisition and Affinity Maturation. J Immunol. 2017;198:4304-4311 pubmed publisher
Grenga I, Donahue R, Lepone L, Richards J, Schlom J. A fully human IgG1 anti-PD-L1 MAb in an in vitro assay enhances antigen-specific T-cell responses. Clin Transl Immunology. 2016;5:e83 pubmed publisher
Meya D, Okurut S, Zziwa G, Rolfes M, Kelsey M, Cose S, et al. Cellular immune activation in cerebrospinal fluid from ugandans with cryptococcal meningitis and immune reconstitution inflammatory syndrome. J Infect Dis. 2015;211:1597-606 pubmed publisher
Kaufmann K, Gründer A, Hadlich T, Wehrle J, Gothwal M, Bogeska R, et al. A novel murine model of myeloproliferative disorders generated by overexpression of the transcription factor NF-E2. J Exp Med. 2012;209:35-50 pubmed publisher
Wang H, Meng R, Li Z, Yang B, Liu Y, Huang F, et al. IL-27 induces the differentiation of Tr1-like cells from human naive CD4+ T cells via the phosphorylation of STAT1 and STAT3. Immunol Lett. 2011;136:21-8 pubmed publisher
product information
Antigen :
CD16
Apps. Abbrev. :
Flow Cytometry
Cat # :
302011
Clone :
3G8
Item :
APC anti-human CD16
Isotype :
Mouse IgG1, κ
Other Names :
FcγRIII, Fc gamma receptor, Fc gamma receptor 3
Size :
25 tests
Price (USD) :
132 USD
Reactivity :
Human, Cynomolgus, Rhesus
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
APC
Immunogen :
Human PMN cells
Application Notes :
The 3G8 antibody clone blocks neutrophil phagocytosis and stimulates NK cell proliferation. It has been reported that this clone interacts with the Fc RIIa and Fc RIIIb receptors causing neutrophil activation and aggregation 18 . Due to this phenomenon staining in whole blood may cause a reduction in the number of granulocytes or alter their scatter profile. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections 6 , immunoprecipitation 3 , stimulation of NK cell proliferation 4 , blocking of phagocytosis 5 , and blocking of immunoglobulin binding to Fc RIII 7,8 . The Ultra-LEAF purified antibody (Endotoxin 0.01 EU/ug, Azide-Free, 0.2 um filtered) is recommended for functional assays (Cat. No. 302049, 302050, 302057, 302058).
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA