PE/Cyanine5 anti-human CD16 | BioLegend 302010 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

PE/Cyanine5 anti-human CD16

catalog :

302010

quantity :

100 tests

price :

297 USD

clonality :

monoclonal

host :

mouse

conjugate :

PE-Cy5

clone name :

3G8

reactivity :

human, rhesus macaque

application :

flow cytometry

more info or order :

BioLegend product webpage

citations: 14

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; loading ...	Demers K, Makedonas G, Buggert M, Eller M, Ratcliffe S, Goonetilleke N, et al. Temporal Dynamics of CD8+ T Cell Effector Responses during Primary HIV Infection. PLoS Pathog. 2016;12:e1005805 pubmed publisher
flow cytometry; human	Bendall S, Simonds E, Qiu P, Amir E, Krutzik P, Finck R, et al. Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum. Science. 2011;332:687-96 pubmed publisher
	Wang M, Yan Y, Zhang Z, Yao X, Duan X, Jiang Z, et al. Programmed PPAR-α downregulation induces inflammaging by suppressing fatty acid catabolism in monocytes. iScience. 2021;24:102766 pubmed publisher
	Swanson E, Lord C, Reading J, Heubeck A, Genge P, Thomson Z, et al. Simultaneous trimodal single-cell measurement of transcripts, epitopes, and chromatin accessibility using TEA-seq. elife. 2021;10: pubmed publisher
	Mpakou V, Spathis A, Bouhla A, Mpazani E, Papageorgiou S, Gkontopoulos K, et al. Synergistic inhibitory effects of low-dose decitabine in combination with bortezomib in the AML cell line Kasumi-1. Exp Ther Med. 2021;21:195 pubmed publisher
	Giron L, Colomb F, Papasavvas E, Azzoni L, Yin X, Fair M, et al. Interferon-α alters host glycosylation machinery during treated HIV infection. EBioMedicine. 2020;59:102945 pubmed publisher
	Gupta R, Turati V, Brian D, Thrussel C, Wilbourn B, May G, et al. Nov/CCN3 Enhances Cord Blood Engraftment by Rapidly Recruiting Latent Human Stem Cell Activity. Cell Stem Cell. 2020;26:527-541.e8 pubmed publisher
	Ye Q, Chourey S, Reddy C, Wang R, Cossette C, Gravel S, et al. Novel highly potent OXE receptor antagonists with prolonged plasma lifetimes that are converted to active metabolites in vivo in monkeys. Br J Pharmacol. 2020;177:388-401 pubmed publisher
	Manser A, Scherenschlich N, Thöns C, Hengel H, Timm J, Uhrberg M. KIR Polymorphism Modulates the Size of the Adaptive NK Cell Pool in Human Cytomegalovirus-Infected Individuals. J Immunol. 2019;203:2301-2309 pubmed publisher
	Shao T, Ang W, Jiang T, Huang F, Andersen H, Kinder J, et al. Commensal Candida albicans Positively Calibrates Systemic Th17 Immunological Responses. Cell Host Microbe. 2019;25:404-417.e6 pubmed publisher
	Barisione C, Garibaldi S, Furfaro A, Nitti M, Palmieri D, Passalacqua M, et al. Moderate Increase of Indoxyl Sulfate Promotes Monocyte Transition into Profibrotic Macrophages. PLoS ONE. 2016;11:e0149276 pubmed publisher
	Foster G, Xu L, Chidambaram A, Soderberg S, Armstrong E, Wu H, et al. CD11c/CD18 Signals Very Late Antigen-4 Activation To Initiate Foamy Monocyte Recruitment during the Onset of Hypercholesterolemia. J Immunol. 2015;195:5380-92 pubmed publisher
	Sen A, Chowdhury I, Mukhopadhyay D, Paine S, Mukherjee A, Mondal L, et al. Increased Toll-like receptor-2 expression on nonclassic CD16+ monocytes from patients with inflammatory stage of Eales' disease. Invest Ophthalmol Vis Sci. 2011;52:6940-8 pubmed publisher
	Huang Y, Zhu X, Du M, Li D. Human trophoblasts recruited T lymphocytes and monocytes into decidua by secretion of chemokine CXCL16 and interaction with CXCR6 in the first-trimester pregnancy. J Immunol. 2008;180:2367-75 pubmed

product information

Antigen :

CD16

Apps. Abbrev. :

Flow Cytometry

Cat # :

302010

Clone :

3G8

Item :

PE/Cyanine5 anti-human CD16

Isotype :

Mouse IgG1, κ

Other Names :

FcγRIII, Fc gamma receptor, Fc gamma receptor 3

Size :

100 tests

Price (USD) :

297 USD

Reactivity :

Human, Cynomolgus, Rhesus

Clonality :

Monoclonal

Host :

Mouse

Conjugate/Tag/Label :

PE_CYANINE5

Immunogen :

Human PMN cells

Application Notes :

The 3G8 antibody clone blocks neutrophil phagocytosis and stimulates NK cell proliferation. It has been reported that this clone interacts with the Fc RIIa and Fc RIIIb receptors causing neutrophil activation and aggregation 18 . Due to this phenomenon staining in whole blood may cause a reduction in the number of granulocytes or alter their scatter profile. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections 6 , immunoprecipitation 3 , stimulation of NK cell proliferation 4 , blocking of phagocytosis 5 , and blocking of immunoglobulin binding to Fc RIII 7,8 . The Ultra-LEAF purified antibody (Endotoxin 0.01 EU/ug, Azide-Free, 0.2 um filtered) is recommended for functional assays (Cat. No. 302049, 302050, 302057, 302058).

more info or order :

BioLegend product webpage