product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
FITC anti-human CD16
catalog :
302005
quantity :
25 tests
price :
93 USD
clonality :
monoclonal
host :
mouse
conjugate :
FITC
clone name :
3G8
reactivity :
human, rhesus macaque
application :
flow cytometry, immunohistochemistry - frozen section
more info or order :
citations: 38
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human
Jitschin R, Braun M, Büttner M, Dettmer Wilde K, Bricks J, Berger J, et al. CLL-cells induce IDOhi CD14+HLA-DRlo myeloid-derived suppressor cells that inhibit T-cell responses and promote TRegs. Blood. 2014;124:750-60 pubmed publisher
  • immunohistochemistry - frozen section; rhesus macaque; loading ...
Chicoine L, Rodino Klapac L, Shao G, Xu R, Bremer W, Camboni M, et al. Vascular delivery of rAAVrh74.MCK.GALGT2 to the gastrocnemius muscle of the rhesus macaque stimulates the expression of dystrophin and laminin ?2 surrogates. Mol Ther. 2014;22:713-24 pubmed publisher
  • flow cytometry; human
Bendall S, Simonds E, Qiu P, Amir E, Krutzik P, Finck R, et al. Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum. Science. 2011;332:687-96 pubmed publisher
Hasaart K, Manders F, Ubels J, Verheul M, van Roosmalen M, Groenen N, et al. Human induced pluripotent stem cells display a similar mutation burden as embryonic pluripotent cells in vivo. iScience. 2022;25:103736 pubmed publisher
Sefik E, Israelow B, Mirza H, Zhao J, Qu R, Kaffe E, et al. A humanized mouse model of chronic COVID-19. Nat Biotechnol. 2021;: pubmed publisher
Brandsma A, Bertrums E, van Roosmalen M, Hofman D, Oka R, Verheul M, et al. Mutation signatures of pediatric acute myeloid leukemia and normal blood progenitors associated with differential patient outcomes. Blood Cancer Discov. 2021;2:484-499 pubmed publisher
Zee B, Poels K, Yao C, Kawabata K, Wu G, Duy C, et al. Combined epigenetic and metabolic treatments overcome differentiation blockade in acute myeloid leukemia. iScience. 2021;24:102651 pubmed publisher
Vanoni G, Ercolano G, Candiani S, Rutigliani M, Lanata M, Derre L, et al. Human primed ILCPs support endothelial activation through NF-κB signaling. elife. 2021;10: pubmed publisher
Evren E, Ringqvist E, Tripathi K, Sleiers N, Rives I, Alisjahbana A, et al. Distinct developmental pathways from blood monocytes generate human lung macrophage diversity. Immunity. 2021;54:259-275.e7 pubmed publisher
Wang J, Xu Y, Chen Z, Liang J, Lin Z, Liang H, et al. Liver Immune Profiling Reveals Pathogenesis and Therapeutics for Biliary Atresia. Cell. 2020;183:1867-1883.e26 pubmed publisher
Tripathi H, Al Darraji A, Abo Aly M, Peng H, Shokri E, Chelvarajan L, et al. Autotaxin inhibition reduces cardiac inflammation and mitigates adverse cardiac remodeling after myocardial infarction. J Mol Cell Cardiol. 2020;149:95-114 pubmed publisher
Walter F, Winter E, Rahn S, Heidland J, Meier S, Struzek A, et al. Chitosan nanoparticles as antigen vehicles to induce effective tumor specific T cell responses. PLoS ONE. 2020;15:e0239369 pubmed publisher
Evans R, Antonelou M, Sathiananthamoorthy S, Rega M, Henderson S, Ceron Gutierrez L, et al. Inherited salt-losing tubulopathies are associated with immunodeficiency due to impaired IL-17 responses. Nat Commun. 2020;11:4368 pubmed publisher
Hasaart K, Manders F, van der Hoorn M, Verheul M, Poplonski T, Kuijk E, et al. Mutation accumulation and developmental lineages in normal and Down syndrome human fetal haematopoiesis. Sci Rep. 2020;10:12991 pubmed publisher
Zhang D, Jiang F, Zaynagetdinov R, Huang H, Sood V, Wang H, et al. Identification and characterization of M6903, an antagonistic anti-TIM-3 monoclonal antibody. Oncoimmunology. 2020;9:1744921 pubmed publisher
Seamon K, Kurlak L, Warthan M, Stratikos E, Strauss J, Mistry H, et al. The Differential Expression of ERAP1/ERAP2 and Immune Cell Activation in Pre-eclampsia. Front Immunol. 2020;11:396 pubmed publisher
Sagnella S, Yang L, Stubbs G, Boslem E, Martino Echarri E, Smolarczyk K, et al. Cyto-Immuno-Therapy for Cancer: A Pathway Elicited by Tumor-Targeted, Cytotoxic Drug-Packaged Bacterially Derived Nanocells. Cancer Cell. 2020;37:354-370.e7 pubmed publisher
Mulfaul K, Ozaki E, Fernando N, Brennan K, Chirco K, Connolly E, et al. Toll-like Receptor 2 Facilitates Oxidative Damage-Induced Retinal Degeneration. Cell Rep. 2020;30:2209-2224.e5 pubmed publisher
Ren C, Yuan Q, Jian X, Randazzo P, Tang W, Wu D. Small GTPase ARF6 Is a Coincidence-Detection Code for RPH3A Polarization in Neutrophil Polarization. J Immunol. 2020;204:1012-1021 pubmed publisher
Nowak W, Grendas L, Sanmarco L, Estecho I, Arena Á, Eberhardt N, et al. Pro-inflammatory monocyte profile in patients with major depressive disorder and suicide behaviour and how ketamine induces anti-inflammatory M2 macrophages by NMDAR and mTOR. EBioMedicine. 2019;50:290-305 pubmed publisher
Hazenberg M, Haverkate N, van Lier Y, Spits H, Krabbendam L, Bemelman W, et al. Human ectoenzyme-expressing ILC3: immunosuppressive innate cells that are depleted in graft-versus-host disease. Blood Adv. 2019;3:3650-3660 pubmed publisher
Kacherovsky N, Cardle I, Cheng E, Yu J, Baldwin M, Salipante S, et al. Traceless aptamer-mediated isolation of CD8+ T cells for chimeric antigen receptor T-cell therapy. Nat Biomed Eng. 2019;: pubmed publisher
Tong M, Potter J, Mor G, Abrahams V. Lipopolysaccharide-Stimulated Human Fetal Membranes Induce Neutrophil Activation and Release of Vital Neutrophil Extracellular Traps. J Immunol. 2019;: pubmed publisher
Souriant S, Balboa L, Dupont M, Pingris K, Kviatcovsky D, Cougoule C, et al. Tuberculosis Exacerbates HIV-1 Infection through IL-10/STAT3-Dependent Tunneling Nanotube Formation in Macrophages. Cell Rep. 2019;26:3586-3599.e7 pubmed publisher
Wang Y, Chen C, Qi J, Wu F, Guan J, Chen Z, et al. Altered PGE2-EP2 is associated with an excessive immune response in HBV-related acute-on-chronic liver failure. J Transl Med. 2019;17:93 pubmed publisher
Osorio F, Rosendahl Huber A, Oka R, Verheul M, Patel S, Hasaart K, et al. Somatic Mutations Reveal Lineage Relationships and Age-Related Mutagenesis in Human Hematopoiesis. Cell Rep. 2018;25:2308-2316.e4 pubmed publisher
Gärtner K, Battke C, Dünzkofer J, Hüls C, von Neubeck B, Kellner M, et al. Tumor-derived extracellular vesicles activate primary monocytes. Cancer Med. 2018;7:2013-2020 pubmed publisher
Pahl J, Koch J, Götz J, Arnold A, Reusch U, Gantke T, et al. CD16A Activation of NK Cells Promotes NK Cell Proliferation and Memory-Like Cytotoxicity against Cancer Cells. Cancer Immunol Res. 2018;6:517-527 pubmed publisher
Wu H, Wiseman R, Hughes C, Webb G, Abdulhaqq S, Bimber B, et al. The Role of MHC-E in T Cell Immunity Is Conserved among Humans, Rhesus Macaques, and Cynomolgus Macaques. J Immunol. 2018;200:49-60 pubmed publisher
Theurich S, Tsaousidou E, Hanssen R, Lempradl A, Mauer J, Timper K, et al. IL-6/Stat3-Dependent Induction of a Distinct, Obesity-Associated NK Cell Subpopulation Deteriorates Energy and Glucose Homeostasis. Cell Metab. 2017;26:171-184.e6 pubmed publisher
Geng S, Chen K, Yuan R, Peng L, Maitra U, Diao N, et al. The persistence of low-grade inflammatory monocytes contributes to aggravated atherosclerosis. Nat Commun. 2016;7:13436 pubmed publisher
Belkina A, Snyder Cappione J. OMIP-037: 16-color panel to measure inhibitory receptor signatures from multiple human immune cell subsets. Cytometry A. 2017;91:175-179 pubmed publisher
Bal S, Bernink J, Nagasawa M, Groot J, Shikhagaie M, Golebski K, et al. IL-1?, IL-4 and IL-12 control the fate of group 2 innate lymphoid cells in human airway inflammation in the lungs. Nat Immunol. 2016;17:636-45 pubmed publisher
Søndergaard J, Laursen J, Rosholm L, Brix S. Mycobacterium tuberculosis promotes Th17 expansion via regulation of human dendritic cells toward a high CD14 and low IL-12p70 phenotype that reprograms upon exogenous IFN-γ. Int Immunol. 2014;26:705-16 pubmed publisher
Poonia B, Pauza C. Levels of CD56+TIM-3- effector CD8 T cells distinguish HIV natural virus suppressors from patients receiving antiretroviral therapy. PLoS ONE. 2014;9:e88884 pubmed publisher
Xue D, He X, Zhou C, Xu X, Xu R, Xu N. Correlation between CD14+CD16++ monocytes in peripheral blood and hypertriglyceridemia after allograft renal transplantation. Transplant Proc. 2013;45:3279-83 pubmed publisher
Hunn M, Farrand K, Broadley K, Weinkove R, Ferguson P, Miller R, et al. Vaccination with irradiated tumor cells pulsed with an adjuvant that stimulates NKT cells is an effective treatment for glioma. Clin Cancer Res. 2012;18:6446-59 pubmed publisher
Okada T, Tsukano H, Endo M, Tabata M, Miyata K, Kadomatsu T, et al. Synoviocyte-derived angiopoietin-like protein 2 contributes to synovial chronic inflammation in rheumatoid arthritis. Am J Pathol. 2010;176:2309-19 pubmed publisher
product information
Antigen :
CD16
Apps. Abbrev. :
Flow Cytometry
Cat # :
302005
Clone :
3G8
Item :
FITC anti-human CD16
Isotype :
Mouse IgG1, κ
Other Names :
FcγRIII, Fc gamma receptor, Fc gamma receptor 3
Size :
25 tests
Price (USD) :
93 USD
Reactivity :
Human, Cynomolgus, Rhesus
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
FITC
Immunogen :
Human PMN cells
Application Notes :
The 3G8 antibody clone blocks neutrophil phagocytosis and stimulates NK cell proliferation. It has been reported that this clone interacts with the Fc RIIa and Fc RIIIb receptors causing neutrophil activation and aggregation 18 . Due to this phenomenon staining in whole blood may cause a reduction in the number of granulocytes or alter their scatter profile. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections 6 , immunoprecipitation 3 , stimulation of NK cell proliferation 4 , blocking of phagocytosis 5 , and blocking of immunoglobulin binding to Fc RIII 7,8 . The Ultra-LEAF purified antibody (Endotoxin 0.01 EU/ug, Azide-Free, 0.2 um filtered) is recommended for functional assays (Cat. No. 302049, 302050, 302057, 302058).
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA