product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Purified anti-human CD16
catalog :
302001
quantity :
25 μg
price :
65 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
3G8
reactivity :
human, mouse, rhesus macaque
application :
neutralization, immunoprecipitation, flow cytometry, immunohistochemistry - frozen section, blocking or activating experiments
more info or order :
citations: 77
Published Application/Species/Sample/DilutionReference
  • blocking or activating experiments; human; 5 ug/ml; loading ...; fig s4
Zhu Y, Xie J, Shi J. Rac1/ROCK-driven membrane dynamics promote natural killer cell cytotoxicity via granzyme-induced necroptosis. BMC Biol. 2021;19:140 pubmed publisher
  • flow cytometry; human; loading ...; fig 4b
Muhammad F, Wang D, Montieth A, Lee S, Preble J, Foster C, et al. PD-1+ melanocortin receptor dependent-Treg cells prevent autoimmune disease. Sci Rep. 2019;9:16941 pubmed publisher
  • flow cytometry; rhesus macaque; loading ...; fig 7a
Kasturi S, Kozlowski P, Nakaya H, Burger M, Russo P, Pham M, et al. Adjuvanting a Simian Immunodeficiency Virus Vaccine with Toll-Like Receptor Ligands Encapsulated in Nanoparticles Induces Persistent Antibody Responses and Enhanced Protection in TRIM5α Restrictive Macaques. J Virol. 2017;91: pubmed publisher
  • flow cytometry; human; loading ...
Demers K, Makedonas G, Buggert M, Eller M, Ratcliffe S, Goonetilleke N, et al. Temporal Dynamics of CD8+ T Cell Effector Responses during Primary HIV Infection. PLoS Pathog. 2016;12:e1005805 pubmed publisher
  • flow cytometry; mouse; 1:200
Ludigs K, Jandus C, Utzschneider D, Staehli F, Bessoles S, Dang A, et al. NLRC5 shields T lymphocytes from NK-cell-mediated elimination under inflammatory conditions. Nat Commun. 2016;7:10554 pubmed publisher
  • flow cytometry; human; fig 5
Westman J, Papareddy P, Dahlgren M, Chakrakodi B, Norrby Teglund A, Smeds E, et al. Extracellular Histones Induce Chemokine Production in Whole Blood Ex Vivo and Leukocyte Recruitment In Vivo. PLoS Pathog. 2015;11:e1005319 pubmed publisher
  • flow cytometry; human; fig 7
Venkatasubramanian S, Tripathi D, Tucker T, Paidipally P, Cheekatla S, Welch E, et al. Tissue factor expression by myeloid cells contributes to protective immune response against Mycobacterium tuberculosis infection. Eur J Immunol. 2016;46:464-79 pubmed publisher
  • flow cytometry; human; loading ...; fig s1b
Schulz A, Mälzer J, Domingo C, Jürchott K, Grützkau A, Babel N, et al. Low Thymic Activity and Dendritic Cell Numbers Are Associated with the Immune Response to Primary Viral Infection in Elderly Humans. J Immunol. 2015;195:4699-711 pubmed publisher
  • flow cytometry; mouse
Amos J, Himes J, Armand L, Gurley T, Martinez D, Colvin L, et al. Rapid Development of gp120-Focused Neutralizing B Cell Responses during Acute Simian Immunodeficiency Virus Infection of African Green Monkeys. J Virol. 2015;89:9485-98 pubmed publisher
  • flow cytometry; human; fig s3
Mende N, Kuchen E, Lesche M, Grinenko T, Kokkaliaris K, Hanenberg H, et al. CCND1-CDK4-mediated cell cycle progression provides a competitive advantage for human hematopoietic stem cells in vivo. J Exp Med. 2015;212:1171-83 pubmed publisher
  • flow cytometry; human; fig 1
O Brien E, Abdulahad W, Rutgers A, Huitema M, O Reilly V, Coughlan A, et al. Intermediate monocytes in ANCA vasculitis: increased surface expression of ANCA autoantigens and IL-1β secretion in response to anti-MPO antibodies. Sci Rep. 2015;5:11888 pubmed publisher
  • flow cytometry; human; fig 4
Weldon A, Moldovan I, Cabling M, Hernandez E, Hsu S, Gonzalez J, et al. Surface APRIL Is Elevated on Myeloid Cells and Is Associated with Disease Activity in Patients with Rheumatoid Arthritis. J Rheumatol. 2015;42:749-59 pubmed publisher
  • flow cytometry; human; fig 1
Hünniger K, Bieber K, Martin R, Lehnert T, Figge M, Löffler J, et al. A second stimulus required for enhanced antifungal activity of human neutrophils in blood is provided by anaphylatoxin C5a. J Immunol. 2015;194:1199-210 pubmed publisher
  • flow cytometry; human; fig 7
Hartung E, Becker M, Bachem A, Reeg N, Jäkel A, Hutloff A, et al. Induction of potent CD8 T cell cytotoxicity by specific targeting of antigen to cross-presenting dendritic cells in vivo via murine or human XCR1. J Immunol. 2015;194:1069-79 pubmed publisher
  • flow cytometry; human
Bendall S, Simonds E, Qiu P, Amir E, Krutzik P, Finck R, et al. Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum. Science. 2011;332:687-96 pubmed publisher
Shemesh A, Su Y, Calabrese D, Chen D, Arakawa Hoyt J, Roybal K, et al. Diminished cell proliferation promotes natural killer cell adaptive-like phenotype by limiting FcεRIγ expression. J Exp Med. 2022;219: pubmed publisher
Kirolos S, Pilling D, Gomer R. The extracellular sialidase NEU3 primes neutrophils. J Leukoc Biol. 2022;112:1399-1411 pubmed publisher
Farhadian S, Lindenbaum O, Zhao J, Corley M, Im Y, Walsh H, et al. HIV viral transcription and immune perturbations in the CNS of people with HIV despite ART. JCI Insight. 2022;7: pubmed publisher
Shemesh A, Pickering H, Roybal K, Lanier L. Differential IL-12 signaling induces human natural killer cell activating receptor-mediated ligand-specific expansion. J Exp Med. 2022;219: pubmed publisher
McCarthy E, Odorizzi P, Lutz E, Smullin C, Tenvooren I, Stone M, et al. A cytotoxic-skewed immune set point predicts low neutralizing antibody levels after Zika virus infection. Cell Rep. 2022;39:110815 pubmed publisher
Mask E, Hodara V, Callery J, Parodi L, Obregon Perko V, Yagi S, et al. Molecular Approaches for the Validation of the Baboon as a Nonhuman Primate Model for the Study of Zika Virus Infection. Front Cell Infect Microbiol. 2022;12:880860 pubmed publisher
Park Y, Acosta D, Vassell R, Tang J, Khurana S, Weiss C, et al. D-dimer and CoV-2 spike-immune complexes contribute to the production of PGE2 and proinflammatory cytokines in monocytes. PLoS Pathog. 2022;18:e1010468 pubmed publisher
Alpert A, Nahman O, Starosvetsky E, Hayun M, Curiel T, Ofran Y, et al. Alignment of single-cell trajectories by tuMap enables high-resolution quantitative comparison of cancer samples. Cell Syst. 2022;13:71-82.e8 pubmed publisher
Holder K, Burt K, Grant M. TIGIT blockade enhances NK cell activity against autologous HIV-1-infected CD4+ T cells. Clin Transl Immunology. 2021;10:e1348 pubmed publisher
Syrimi E, Fennell E, Richter A, Vrljicak P, Stark R, Ott S, et al. The immune landscape of SARS-CoV-2-associated Multisystem Inflammatory Syndrome in Children (MIS-C) from acute disease to recovery. iScience. 2021;24:103215 pubmed publisher
Alcántara Hernández M, Idoyaga J. Mass cytometry profiling of human dendritic cells in blood and tissues. Nat Protoc. 2021;16:4855-4877 pubmed publisher
Doni A, Parente R, Laface I, Magrini E, Cunha C, Colombo F, et al. Serum amyloid P component is an essential element of resistance against Aspergillus fumigatus. Nat Commun. 2021;12:3739 pubmed publisher
Kato M, Ikeda K, Sugiyama T, Tanaka S, Iida K, Suga K, et al. Associations of ultrasound-based inflammation patterns with peripheral innate lymphoid cell populations, serum cytokines/chemokines, and treatment response to methotrexate in rheumatoid arthritis and spondyloarthritis. PLoS ONE. 2021;16:e0252116 pubmed publisher
Okubo K, Brenner M, Cullere X, Saggu G, Patchen M, Bose N, et al. Inhibitory affinity modulation of FcγRIIA ligand binding by glycosphingolipids by inside-out signaling. Cell Rep. 2021;35:109142 pubmed publisher
Friedman D, Simmonds P, Hale A, Bere L, Hodson N, White M, et al. Natural killer cell immune synapse formation and cytotoxicity are controlled by tension of the target interface. J Cell Sci. 2021;134: pubmed publisher
Chevrier S, Zurbuchen Y, Cervia C, Adamo S, Raeber M, de Souza N, et al. A distinct innate immune signature marks progression from mild to severe COVID-19. Cell Rep Med. 2021;2:100166 pubmed publisher
Combes A, Courau T, Kuhn N, Hu K, Ray A, Chen W, et al. Global absence and targeting of protective immune states in severe COVID-19. Nature. 2021;591:124-130 pubmed publisher
Li J, Whelan S, Kotturi M, Meyran D, D Souza C, Hansen K, et al. PVRIG is a novel natural killer cell immune checkpoint receptor in acute myeloid leukemia. Haematologica. 2021;106:3115-3124 pubmed publisher
Farrington L, Callaway P, Vance H, Baskevitch K, Lutz E, Warrier L, et al. Opsonized antigen activates Vδ2+ T cells via CD16/FCγRIIIa in individuals with chronic malaria exposure. PLoS Pathog. 2020;16:e1008997 pubmed publisher
Korn M, Schmitt H, Angermüller S, Chambers D, Seeling M, Lux U, et al. Siglec-15 on Osteoclasts Is Crucial for Bone Erosion in Serum-Transfer Arthritis. J Immunol. 2020;205:2595-2605 pubmed publisher
Mann E, Menon M, Knight S, Konkel J, Jagger C, Shaw T, et al. Longitudinal immune profiling reveals key myeloid signatures associated with COVID-19. Sci Immunol. 2020;5: pubmed publisher
Park J, Kim S, Keam B, Park H, Kim S, Kim M, et al. Anti-tumor effects of NK cells and anti-PD-L1 antibody with antibody-dependent cellular cytotoxicity in PD-L1-positive cancer cell lines. J Immunother Cancer. 2020;8: pubmed publisher
Lakshmikanth T, Muhammad S, Olin A, Chen Y, Mikes J, Fagerberg L, et al. Human Immune System Variation during 1 Year. Cell Rep. 2020;32:107923 pubmed publisher
Ehinger E, Ghosheh Y, Pramod A, Lin J, Hanna D, Mueller K, et al. Classical monocyte transcriptomes reveal significant anti-inflammatory statin effect in women with chronic HIV. Cardiovasc Res. 2021;117:1166-1177 pubmed publisher
Weinhage T, K xf6 lsche T, Rieger Fackeldey E, Schmitz R, Antoni A, Ahlmann M, et al. Cord Blood Low-Density Granulocytes Correspond to an Immature Granulocytic Subset with Low Expression of S100A12. J Immunol. 2020;205:56-66 pubmed publisher
De Maeyer R, van de Merwe R, Louie R, Bracken O, Devine O, Goldstein D, et al. Blocking elevated p38 MAPK restores efferocytosis and inflammatory resolution in the elderly. Nat Immunol. 2020;21:615-625 pubmed publisher
Martin E, Minet N, Boschat A, Sanquer S, Sobrino S, Lenoir C, et al. Impaired lymphocyte function and differentiation in CTPS1-deficient patients result from a hypomorphic homozygous mutation. JCI Insight. 2020;5: pubmed publisher
Dorsch M, Urlaub D, B xf6 nnemann V, Br xf6 de P, Sandusky M, Watzl C. Quantitative analysis of human NK cell reactivity using latex beads coated with defined amounts of antibodies. Eur J Immunol. 2020;50:656-665 pubmed publisher
Eccles J, Turner R, Kirk N, Muehling L, Borish L, Steinke J, et al. T-bet+ Memory B Cells Link to Local Cross-Reactive IgG upon Human Rhinovirus Infection. Cell Rep. 2020;30:351-366.e7 pubmed publisher
Steffen U, Koeleman C, Sokolova M, Bang H, Kleyer A, Rech J, et al. IgA subclasses have different effector functions associated with distinct glycosylation profiles. Nat Commun. 2020;11:120 pubmed publisher
Auladell M, Nguyen T, Garcillán B, Mackay F, Kedzierska K, Fox A. Distinguishing naive- from memory-derived human B cells during acute responses. Clin Transl Immunology. 2019;8:e01090 pubmed publisher
Ravell J, Matsuda Lennikov M, Chauvin S, Zou J, Biancalana M, Deeb S, et al. Defective glycosylation and multisystem abnormalities characterize the primary immunodeficiency XMEN disease. J Clin Invest. 2020;130:507-522 pubmed publisher
Dutertre C, Becht E, Irac S, Khalilnezhad A, Narang V, Khalilnezhad S, et al. Single-Cell Analysis of Human Mononuclear Phagocytes Reveals Subset-Defining Markers and Identifies Circulating Inflammatory Dendritic Cells. Immunity. 2019;51:573-589.e8 pubmed publisher
Ardain A, Domingo Gonzalez R, Das S, Kazer S, Howard N, Singh A, et al. Group 3 innate lymphoid cells mediate early protective immunity against tuberculosis. Nature. 2019;: pubmed publisher
Wagner J, Rapsomaniki M, Chevrier S, Anzeneder T, Langwieder C, Dykgers A, et al. A Single-Cell Atlas of the Tumor and Immune Ecosystem of Human Breast Cancer. Cell. 2019;177:1330-1345.e18 pubmed publisher
ELDREDGE L, Creasy R, Presnell S, Debley J, Juul S, Mayock D, et al. Infants with evolving bronchopulmonary dysplasia demonstrate monocyte-specific expression of IL-1 in tracheal aspirates. Am J Physiol Lung Cell Mol Physiol. 2019;317:L49-L56 pubmed publisher
Li L, Wang Y, Torkelson J, Shankar G, Pattison J, Zhen H, et al. TFAP2C- and p63-Dependent Networks Sequentially Rearrange Chromatin Landscapes to Drive Human Epidermal Lineage Commitment. Cell Stem Cell. 2019;24:271-284.e8 pubmed publisher
Pohl K, Nichols D, Taylor Cousar J, Saavedra M, Strand M, Nick J, et al. Corticosteroid use and increased CXCR2 levels on leukocytes are associated with lumacaftor/ivacaftor discontinuation in cystic fibrosis patients homozygous for the F508del CFTR mutation. PLoS ONE. 2018;13:e0209026 pubmed publisher
Jog N, Chakravarty E, Guthridge J, James J. Epstein Barr Virus Interleukin 10 Suppresses Anti-inflammatory Phenotype in Human Monocytes. Front Immunol. 2018;9:2198 pubmed publisher
Ravindran A, Rönnberg E, Dahlin J, Mazzurana L, Säfholm J, Orre A, et al. An Optimized Protocol for the Isolation and Functional Analysis of Human Lung Mast Cells. Front Immunol. 2018;9:2193 pubmed publisher
Sung J, Patel S, Clohosey M, Roesch L, Tripic T, Kuruc J, et al. HIV-Specific, Ex Vivo Expanded T Cell Therapy: Feasibility, Safety, and Efficacy in ART-Suppressed HIV-Infected Individuals. Mol Ther. 2018;26:2496-2506 pubmed publisher
Olin A, Henckel E, Chen Y, Lakshmikanth T, Pou C, Mikes J, et al. Stereotypic Immune System Development in Newborn Children. Cell. 2018;174:1277-1292.e14 pubmed publisher
Sibener L, Fernandes R, Kolawole E, Carbone C, Liu F, McAffee D, et al. Isolation of a Structural Mechanism for Uncoupling T Cell Receptor Signaling from Peptide-MHC Binding. Cell. 2018;174:672-687.e27 pubmed publisher
Boutboul D, Kuehn H, Van de Wyngaert Z, Niemela J, Callebaut I, Stoddard J, et al. Dominant-negative IKZF1 mutations cause a T, B, and myeloid cell combined immunodeficiency. J Clin Invest. 2018;128:3071-3087 pubmed publisher
Frobel J, Rahmig S, Franzen J, Waskow C, Wagner W. Epigenetic aging of human hematopoietic cells is not accelerated upon transplantation into mice. Clin Epigenetics. 2018;10:67 pubmed publisher
Zhong Q, Gong F, Gong Z, Hua S, Zeng K, Gao X. IgG Immunocomplexes Sensitize Human Monocytes for Inflammatory Hyperactivity via Transcriptomic and Epigenetic Reprogramming in Rheumatoid Arthritis. J Immunol. 2018;200:3913-3925 pubmed publisher
Chevrier S, Crowell H, Zanotelli V, Engler S, Robinson M, Bodenmiller B. Compensation of Signal Spillover in Suspension and Imaging Mass Cytometry. Cell Syst. 2018;6:612-620.e5 pubmed publisher
Cao Q, Wang Y, Wang C, Wang X, Lee V, Zheng G, et al. Therapeutic potential of regulatory macrophages generated from peritoneal dialysate in adriamycin nephropathy. Am J Physiol Renal Physiol. 2018;314:F561-F571 pubmed publisher
Miyamoto D, Lee R, Kalinich M, LiCausi J, Zheng Y, Chen T, et al. An RNA-Based Digital Circulating Tumor Cell Signature Is Predictive of Drug Response and Early Dissemination in Prostate Cancer. Cancer Discov. 2018;8:288-303 pubmed publisher
Santos R, Bai L, Singh P, Murakami N, Fan H, Zhan W, et al. Structure of human immunoproteasome with a reversible and noncompetitive inhibitor that selectively inhibits activated lymphocytes. Nat Commun. 2017;8:1692 pubmed publisher
Winkler M, Rissiek A, Priefler M, Schwedhelm E, Robbe L, Bauer A, et al. Human leucocyte antigen (HLA-DR) gene expression is reduced in sepsis and correlates with impaired TNF? response: A diagnostic tool for immunosuppression?. PLoS ONE. 2017;12:e0182427 pubmed publisher
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product information
Antigen :
CD16
Apps. Abbrev. :
FC, CyTOF®, Block, IHC-F, IP, Stim
Cat # :
302001
Clone :
3G8
Item :
Purified anti-human CD16
Isotype :
Mouse IgG1, κ
Other Names :
FcγRIII, Fc gamma receptor, Fc gamma receptor 3
Size :
25 μg
Price (USD) :
65 USD
Reactivity :
Human, Cynomolgus, Rhesus
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
PURE
Immunogen :
Human PMN cells
Application Notes :
The 3G8 antibody clone blocks neutrophil phagocytosis and stimulates NK cell proliferation. It has been reported that this clone interacts with the FcγRIIa and FcγRIIIb receptors causing neutrophil activation and aggregation^18. Due to this phenomenon staining in whole blood may cause a reduction in the number of granulocytes or alter their scatter profile.Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections^6, immunoprecipitation^3, stimulation of NK cell proliferation^4, blocking of phagocytosis^5, and blocking of immunoglobulin binding to FcγRIII^7,8. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 302049, 302050, 302057, 302058).;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA