Ultra-LEAF™ Purified anti-human CD14 | BioLegend 301861 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

Ultra-LEAF™ Purified anti-human CD14

catalog :

301861

quantity :

100 μg

price :

237 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

M5E2

reactivity :

human, rhesus macaque

application :

neutralization, flow cytometry, immunohistochemistry - frozen section, mass cytometry

more info or order :

BioLegend product webpage

citations: 13

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; 1:100; loading ...; fig 7c	Beider K, Voevoda Dimenshtein V, Zoabi A, Rosenberg E, Magen H, Ostrovsky O, et al. CXCL13 chemokine is a novel player in multiple myeloma osteolytic microenvironment, M2 macrophage polarization, and tumor progression. J Hematol Oncol. 2022;15:144 pubmed publisher
flow cytometry; human; loading ...	Amaral E, Foreman T, Namasivayam S, Hilligan K, Kauffman K, Barbosa Bomfim C, et al. GPX4 regulates cellular necrosis and host resistance in Mycobacterium tuberculosis infection. J Exp Med. 2022;219: pubmed publisher
flow cytometry; human	Chiou S, Tseng D, Reuben A, Mallajosyula V, Molina I, Conley S, et al. Global analysis of shared T cell specificities in human non-small cell lung cancer enables HLA inference and antigen discovery. Immunity. 2021;54:586-602.e8 pubmed publisher
flow cytometry; human; loading ...; fig 3s2a	Gregorova M, Morse D, Brignoli T, Steventon J, Hamilton F, Albur M, et al. Post-acute COVID-19 associated with evidence of bystander T-cell activation and a recurring antibiotic-resistant bacterial pneumonia. elife. 2020;9: pubmed publisher
flow cytometry; human; loading ...; fig s1b	Martin E, Minet N, Boschat A, Sanquer S, Sobrino S, Lenoir C, et al. Impaired lymphocyte function and differentiation in CTPS1-deficient patients result from a hypomorphic homozygous mutation. JCI Insight. 2020;5: pubmed publisher
flow cytometry; human; loading ...; fig 4a	Muhammad F, Wang D, Montieth A, Lee S, Preble J, Foster C, et al. PD-1+ melanocortin receptor dependent-Treg cells prevent autoimmune disease. Sci Rep. 2019;9:16941 pubmed publisher
flow cytometry; human; loading ...; fig ex2a	Barkal A, Brewer R, Markovic M, Kowarsky M, Barkal S, Zaro B, et al. CD24 signalling through macrophage Siglec-10 is a target for cancer immunotherapy. Nature. 2019;572:392-396 pubmed publisher
flow cytometry; human; loading ...; fig 3a	Allden S, Ogger P, Ghai P, McErlean P, Hewitt R, Toshner R, et al. The Transferrin Receptor CD71 Delineates Functionally Distinct Airway Macrophage Subsets during Idiopathic Pulmonary Fibrosis. Am J Respir Crit Care Med. 2019;: pubmed publisher
mass cytometry; human; loading ...; fig s1	Fernandez I, Baxter R, Garcia Perez J, Vendrame E, Ranganath T, Kong D, et al. A novel human IL2RB mutation results in T and NK cell-driven immune dysregulation. J Exp Med. 2019;216:1255-1267 pubmed publisher
flow cytometry; human; loading ...; fig 5	Oda H, Beck D, Kuehn H, Sampaio Moura N, Hoffmann P, Ibarra M, et al. Second Case of HOIP Deficiency Expands Clinical Features and Defines Inflammatory Transcriptome Regulated by LUBAC. Front Immunol. 2019;10:479 pubmed publisher
flow cytometry; human; 1:100; loading ...; fig 1j	Mouhadeb O, Ben Shlomo S, Cohen K, Farkash I, Gruber S, Maharshak N, et al. Impaired COMMD10-Mediated Regulation of Ly6Chi Monocyte-Driven Inflammation Disrupts Gut Barrier Function. Front Immunol. 2018;9:2623 pubmed publisher
	Kong X, Xu J, Hu Y, Jiao Y, Chen S, Yu C, et al. Circulation immune cell landscape in canonical pathogenesis of colorectal adenocarcinoma by CyTOF analysis. iScience. 2024;27:109229 pubmed publisher
	Thrash E, Kleinsteuber K, Hathaway E, Nazzaro M, Haas E, Hodi F, et al. High-Throughput Mass Cytometry Staining for Immunophenotyping Clinical Samples. STAR Protoc. 2020;1:100055 pubmed publisher

product information

Antigen :

CD14

Apps. Abbrev. :

FC, CyTOF®, Block, IHC-F

Cat # :

301861

Clone :

M5E2

Item :

Ultra-LEAF™ Purified anti-human CD14

Isotype :

Mouse IgG2a, κ

Other Names :

LPS receptor

Size :

100 μg

Price (USD) :

237 USD

Reactivity :

Human, Cynomolgus, Rhesus

Clonality :

Monoclonal

Host :

Mouse

Conjugate/Tag/Label :

ULEAF

Immunogen :

Full-length human CD14 protein

Application Notes :

The M5E2 antibody inhibits monocyte activation and cytokine production induced by LPS. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections, blocking of LPS stimulation^4, and immunofluorescence microscopy^5. Clone M5E2 is not recommended for immunohistochemical staining of formalin-fixed paraffin-embedded sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 301861 and 301862).;

more info or order :

BioLegend product webpage