product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
TotalSeq™-A0081 anti-human CD14
catalog :
301855
quantity :
10 μg
price :
369 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
M5E2
reactivity :
African green monkey, human, rhesus macaque
application :
flow cytometry, other, mass cytometry
more info or order :
citations: 25
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; 1:100; loading ...; fig 7c
Beider K, Voevoda Dimenshtein V, Zoabi A, Rosenberg E, Magen H, Ostrovsky O, et al. CXCL13 chemokine is a novel player in multiple myeloma osteolytic microenvironment, M2 macrophage polarization, and tumor progression. J Hematol Oncol. 2022;15:144 pubmed publisher
  • flow cytometry; human; loading ...
Amaral E, Foreman T, Namasivayam S, Hilligan K, Kauffman K, Barbosa Bomfim C, et al. GPX4 regulates cellular necrosis and host resistance in Mycobacterium tuberculosis infection. J Exp Med. 2022;219: pubmed publisher
  • flow cytometry; human
Chiou S, Tseng D, Reuben A, Mallajosyula V, Molina I, Conley S, et al. Global analysis of shared T cell specificities in human non-small cell lung cancer enables HLA inference and antigen discovery. Immunity. 2021;54:586-602.e8 pubmed publisher
  • flow cytometry; human; loading ...; fig 3s2a
Gregorova M, Morse D, Brignoli T, Steventon J, Hamilton F, Albur M, et al. Post-acute COVID-19 associated with evidence of bystander T-cell activation and a recurring antibiotic-resistant bacterial pneumonia. elife. 2020;9: pubmed publisher
  • flow cytometry; human; loading ...; fig s1b
Martin E, Minet N, Boschat A, Sanquer S, Sobrino S, Lenoir C, et al. Impaired lymphocyte function and differentiation in CTPS1-deficient patients result from a hypomorphic homozygous mutation. JCI Insight. 2020;5: pubmed publisher
  • flow cytometry; human; loading ...; fig 4a
Muhammad F, Wang D, Montieth A, Lee S, Preble J, Foster C, et al. PD-1+ melanocortin receptor dependent-Treg cells prevent autoimmune disease. Sci Rep. 2019;9:16941 pubmed publisher
  • flow cytometry; human; loading ...; fig ex2a
Barkal A, Brewer R, Markovic M, Kowarsky M, Barkal S, Zaro B, et al. CD24 signalling through macrophage Siglec-10 is a target for cancer immunotherapy. Nature. 2019;572:392-396 pubmed publisher
  • other; human; loading ...; fig 4b
Stuart T, Butler A, Hoffman P, Hafemeister C, Papalexi E, Mauck W, et al. Comprehensive Integration of Single-Cell Data. Cell. 2019;: pubmed publisher
  • flow cytometry; human; loading ...; fig 3a
Allden S, Ogger P, Ghai P, McErlean P, Hewitt R, Toshner R, et al. The Transferrin Receptor CD71 Delineates Functionally Distinct Airway Macrophage Subsets during Idiopathic Pulmonary Fibrosis. Am J Respir Crit Care Med. 2019;: pubmed publisher
  • mass cytometry; human; loading ...; fig s1
Fernandez I, Baxter R, Garcia Perez J, Vendrame E, Ranganath T, Kong D, et al. A novel human IL2RB mutation results in T and NK cell-driven immune dysregulation. J Exp Med. 2019;216:1255-1267 pubmed publisher
  • flow cytometry; human; loading ...; fig s1b
Zhang J, Supakorndej T, Krambs J, Rao M, Abou Ezzi G, Ye R, et al. Bone marrow dendritic cells regulate hematopoietic stem/progenitor cell trafficking. J Clin Invest. 2019;129:2920-2931 pubmed publisher
  • flow cytometry; human; loading ...; fig 5
Oda H, Beck D, Kuehn H, Sampaio Moura N, Hoffmann P, Ibarra M, et al. Second Case of HOIP Deficiency Expands Clinical Features and Defines Inflammatory Transcriptome Regulated by LUBAC. Front Immunol. 2019;10:479 pubmed publisher
  • flow cytometry; human; 1:200; loading ...; fig 1b
Jones G, Bain C, Fenton T, Kelly A, Brown S, Ivens A, et al. Dynamics of Colon Monocyte and Macrophage Activation During Colitis. Front Immunol. 2018;9:2764 pubmed publisher
  • flow cytometry; rhesus macaque; loading ...; fig 2d
Chea L, Wyatt L, Gangadhara S, Moss B, Amara R. Novel Modified Vaccinia Virus Ankara Vector Expressing Anti-apoptotic Gene B13R Delays Apoptosis and Enhances Humoral Responses. J Virol. 2019;93: pubmed publisher
  • flow cytometry; human; 1:100; loading ...; fig 1j
Mouhadeb O, Ben Shlomo S, Cohen K, Farkash I, Gruber S, Maharshak N, et al. Impaired COMMD10-Mediated Regulation of Ly6Chi Monocyte-Driven Inflammation Disrupts Gut Barrier Function. Front Immunol. 2018;9:2623 pubmed publisher
  • flow cytometry; human; loading ...; fig 2c
Clayton K, Collins D, Lengieza J, Ghebremichael M, Dotiwala F, Lieberman J, et al. Resistance of HIV-infected macrophages to CD8+ T lymphocyte-mediated killing drives activation of the immune system. Nat Immunol. 2018;19:475-486 pubmed publisher
  • flow cytometry; human; loading ...; fig s1
Melo Gonzalez F, Fenton T, Forss C, Smedley C, Goenka A, MacDonald A, et al. Intestinal mucin activates human dendritic cells and IL-8 production in a glycan-specific manner. J Biol Chem. 2018;293:8543-8553 pubmed publisher
  • flow cytometry; African green monkey; loading ...; fig 2a
Holbrook B, Aycock S, Machiele E, Clemens E, Gries D, Jorgensen M, et al. An R848 adjuvanted influenza vaccine promotes early activation of B cells in the draining lymph nodes of non-human primate neonates. Immunology. 2018;153:357-367 pubmed publisher
Hao Y, Hao S, Andersen Nissen E, Mauck W, Zheng S, Butler A, et al. Integrated analysis of multimodal single-cell data. Cell. 2021;184:3573-3587.e29 pubmed publisher
Swanson E, Lord C, Reading J, Heubeck A, Genge P, Thomson Z, et al. Simultaneous trimodal single-cell measurement of transcripts, epitopes, and chromatin accessibility using TEA-seq. elife. 2021;10: pubmed publisher
Cadot S, Valle C, Tosolini M, Pont F, Largeaud L, Laurent C, et al. Longitudinal CITE-Seq profiling of chronic lymphocytic leukemia during ibrutinib treatment: evolution of leukemic and immune cells at relapse. Biomark Res. 2020;8:72 pubmed publisher
Witkowski M, Dolgalev I, Evensen N, Ma C, Chambers T, Roberts K, et al. Extensive Remodeling of the Immune Microenvironment in B Cell Acute Lymphoblastic Leukemia. Cancer Cell. 2020;37:867-882.e12 pubmed publisher
Zizzo G, Hilliard B, Monestier M, Cohen P. Efficient clearance of early apoptotic cells by human macrophages requires M2c polarization and MerTK induction. J Immunol. 2012;189:3508-20 pubmed
Santer D, Hall B, George T, Tangsombatvisit S, Liu C, Arkwright P, et al. C1q deficiency leads to the defective suppression of IFN-alpha in response to nucleoprotein containing immune complexes. J Immunol. 2010;185:4738-49 pubmed publisher
Iwamoto S, Iwai S, Tsujiyama K, Kurahashi C, Takeshita K, Naoe M, et al. TNF-alpha drives human CD14+ monocytes to differentiate into CD70+ dendritic cells evoking Th1 and Th17 responses. J Immunol. 2007;179:1449-57 pubmed
product information
Antigen :
CD14
Apps. Abbrev. :
PG
Cat # :
301855
Clone :
M5E2
Item :
TotalSeq™-A0081 anti-human CD14
Isotype :
Mouse IgG2a, κ
Other Names :
LPS receptor
Size :
10 μg
Price (USD) :
369 USD
Reactivity :
Human, Cynomolgus, Rhesus
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
TOTALSEQ_A
Immunogen :
Full-length human CD14 protein
Application Notes :
The M5E2 antibody inhibits monocyte activation and cytokine production induced by LPS. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections, blocking of LPS stimulation^4, and immunofluorescence microscopy^5. Clone M5E2 is not recommended for immunohistochemical staining of formalin-fixed paraffin-embedded sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 301861 and 301862).;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA