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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
PE anti-human CD14
catalog :
301850
quantity :
100 μg
price :
146 USD
clonality :
monoclonal
host :
mouse
conjugate :
PE
clone name :
M5E2
reactivity :
human, rhesus macaque
application :
flow cytometry
more info or order :
citations: 51
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human
Bendall S, Simonds E, Qiu P, Amir E, Krutzik P, Finck R, et al. Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum. Science. 2011;332:687-96 pubmed publisher
Li J, Zhang Z, Zhuang Y, Wang F, Cai T. Small RNA transcriptome analysis using parallel single-cell small RNA sequencing. Sci Rep. 2023;13:7501 pubmed publisher
Okano F, Saito T, Minamida Y, Kobayashi S, Ido T, Miyauchi Y, et al. Identification of Membrane-expressed CAPRIN-1 as a Novel and Universal Cancer Target, and Generation of a Therapeutic Anti-CAPRIN-1 Antibody TRK-950. Cancer Res Commun. 2023;3:640-658 pubmed publisher
Wu X, He Y, Chen Z, He Z, Yan Y, He Y, et al. Single-cell analysis of peripheral blood from high-altitude pulmonary hypertension patients identifies a distinct monocyte phenotype. Nat Commun. 2023;14:1820 pubmed publisher
Hamdan F, Feodoroff M, Russo S, Fusciello M, Feola S, Chiaro J, et al. Controlled release of enhanced cross-hybrid IgGA Fc PD-L1 inhibitors using oncolytic adenoviruses. Mol Ther Oncolytics. 2023;28:264-276 pubmed publisher
Reitinger C, Ipsen Escobedo A, Hornung C, Heger L, Dudziak D, Lux A, et al. Modulation of urelumab glycosylation separates immune stimulatory activity from organ toxicity. Front Immunol. 2022;13:970290 pubmed publisher
Polisetti N, Sharaf L, Reinhard T, Schlunck G. Isolation and ex vivo Expansion of Limbal Mesenchymal Stromal Cells. Bio Protoc. 2022;12: pubmed publisher
Gopinath A, Mackie P, Hashimi B, Buchanan A, Smith A, Bouchard R, et al. DAT and TH expression marks human Parkinson's disease in peripheral immune cells. NPJ Parkinsons Dis. 2022;8:72 pubmed publisher
Luo W, Napoleon J, Zhang F, Lee Y, Wang B, Putt K, et al. Repolarization of Tumor-Infiltrating Myeloid Cells for Augmentation of CAR T Cell Therapies. Front Immunol. 2022;13:816761 pubmed publisher
Pinho S, Wei Q, Maryanovich M, Zhang D, Balandr xe1 n J, Pierce H, et al. VCAM1 confers innate immune tolerance on haematopoietic and leukaemic stem cells. Nat Cell Biol. 2022;24:290-298 pubmed publisher
Karantanos T, Teodorescu P, Perkins B, Christodoulou I, Esteb C, Varadhan R, et al. The role of the atypical chemokine receptor CCRL2 in myelodysplastic syndrome and secondary acute myeloid leukemia. Sci Adv. 2022;8:eabl8952 pubmed publisher
Li Y, Zhu Y, Feng S, Ishida Y, Chiu T, Saito T, et al. Macrophages activated by hepatitis B virus have distinct metabolic profiles and suppress the virus via IL-1β to downregulate PPARα and FOXO3. Cell Rep. 2022;38:110284 pubmed publisher
Kerdidani D, Aerakis E, Verrou K, Angelidis I, Douka K, Maniou M, et al. Lung tumor MHCII immunity depends on in situ antigen presentation by fibroblasts. J Exp Med. 2022;219: pubmed publisher
Miyamoto T, Murakami R, Hamanishi J, Tanigaki K, Hosoe Y, Mise N, et al. B7-H3 Suppresses Antitumor Immunity via the CCL2-CCR2-M2 Macrophage Axis and Contributes to Ovarian Cancer Progression. Cancer Immunol Res. 2022;10:56-69 pubmed publisher
Yuan H, Nishikori M, Ueda C, Fujimoto M, Yasumi T, Otsuka Y, et al. A sporadic case of CTLA4 haploinsufficiency manifesting as Epstein-Barr virus-positive diffuse large B-cell lymphoma. J Clin Exp Hematop. 2022;62:52-57 pubmed publisher
O Neill M, Quach H, Pothlichet J, Aquino Y, Bisiaux A, Zidane N, et al. Single-Cell and Bulk RNA-Sequencing Reveal Differences in Monocyte Susceptibility to Influenza A Virus Infection Between Africans and Europeans. Front Immunol. 2021;12:768189 pubmed publisher
Bekere I, Huang J, Schnapp M, Rudolph M, Berneking L, Ruckdeschel K, et al. Yersinia remodels epigenetic histone modifications in human macrophages. PLoS Pathog. 2021;17:e1010074 pubmed publisher
Babania O, Mohammadi S, Yaghoubi E, Sohrabi A, Sadat Seyedhosseini F, Abdolahi N, et al. The expansion of CD14+ CD163+ subpopulation of monocytes and myeloid cells-associated cytokine imbalance; candidate diagnostic biomarkers for celiac disease (CD). J Clin Lab Anal. 2021;35:e23984 pubmed publisher
Hamdan F, Ylösmäki E, Chiaro J, Giannoula Y, Long M, Fusciello M, et al. Novel oncolytic adenovirus expressing enhanced cross-hybrid IgGA Fc PD-L1 inhibitor activates multiple immune effector populations leading to enhanced tumor killing in vitro, in vivo and with patient-derived tumor organoids. J Immunother Cancer. 2021;9: pubmed publisher
Wang X, Cai J, Lin B, Ma M, Tao Y, Zhou Y, et al. GPR34-mediated sensing of lysophosphatidylserine released by apoptotic neutrophils activates type 3 innate lymphoid cells to mediate tissue repair. Immunity. 2021;54:1123-1136.e8 pubmed publisher
Soday L, Potts M, Hunter L, Ravenhill B, Houghton J, Williamson J, et al. Comparative Cell Surface Proteomic Analysis of the Primary Human T Cell and Monocyte Responses to Type I Interferon. Front Immunol. 2021;12:600056 pubmed publisher
Luvanda M, Posch W, Vosper J, Zaderer V, Noureen A, Lass Fl xf6 rl C, et al. Dexamethasone Promotes Aspergillus fumigatus Growth in Macrophages by Triggering M2 Repolarization via Targeting PKM2. J Fungi (Basel). 2021;7: pubmed publisher
Costa M, Serra J, McDevitt T, Cabral J, da Silva C, Ferreira F. Dimethyloxalylglycine, a small molecule, synergistically increases the homing and angiogenic properties of human mesenchymal stromal cells when cultured as 3D spheroids. Biotechnol J. 2021;16:e2000389 pubmed publisher
Wei Z, Zhang X, Yong T, Bie N, Zhan G, Li X, et al. Boosting anti-PD-1 therapy with metformin-loaded macrophage-derived microparticles. Nat Commun. 2021;12:440 pubmed publisher
Dhariwala M, Karthikeyan D, Vasquez K, Farhat S, Weckel A, Taravati K, et al. Developing Human Skin Contains Lymphocytes Demonstrating a Memory Signature. Cell Rep Med. 2020;1:100132 pubmed publisher
Bailey A, Dmytrenko O, Greenberg L, Bredemeyer A, Ma P, Liu J, et al. SARS-CoV-2 Infects Human Engineered Heart Tissues and Models COVID-19 Myocarditis. bioRxiv. 2020;: pubmed publisher
Tripathi H, Al Darraji A, Abo Aly M, Peng H, Shokri E, Chelvarajan L, et al. Autotaxin inhibition reduces cardiac inflammation and mitigates adverse cardiac remodeling after myocardial infarction. J Mol Cell Cardiol. 2020;149:95-114 pubmed publisher
Walter F, Winter E, Rahn S, Heidland J, Meier S, Struzek A, et al. Chitosan nanoparticles as antigen vehicles to induce effective tumor specific T cell responses. PLoS ONE. 2020;15:e0239369 pubmed publisher
Yang C, Ruiz Rosado J, Robledo Avila F, Li Z, Jennings R, Partida Sanchez S, et al. Antibody-Mediated Protection against Staphylococcus aureus Dermonecrosis: Synergy of Toxin Neutralization and Neutrophil Recruitment. J Invest Dermatol. 2021;141:810-820.e8 pubmed publisher
Huang N, Fan X, Zaleta Rivera K, Nguyen T, Zhou J, Luo Y, et al. Natural display of nuclear-encoded RNA on the cell surface and its impact on cell interaction. Genome Biol. 2020;21:225 pubmed publisher
Zhang D, Jiang F, Zaynagetdinov R, Huang H, Sood V, Wang H, et al. Identification and characterization of M6903, an antagonistic anti-TIM-3 monoclonal antibody. Oncoimmunology. 2020;9:1744921 pubmed publisher
Lavaert M, Liang K, Vandamme N, Park J, Roels J, Kowalczyk M, et al. Integrated scRNA-Seq Identifies Human Postnatal Thymus Seeding Progenitors and Regulatory Dynamics of Differentiating Immature Thymocytes. Immunity. 2020;52:1088-1104.e6 pubmed publisher
Weitz J, Jacques Silva C, Qadir M, Umland O, Pereira E, Qureshi F, et al. Secretory Functions of Macrophages in the Human Pancreatic Islet Are Regulated by Endogenous Purinergic Signaling. Diabetes. 2020;69:1206-1218 pubmed publisher
Ravenhill B, Soday L, Houghton J, Antrobus R, Weekes M. Comprehensive cell surface proteomics defines markers of classical, intermediate and non-classical monocytes. Sci Rep. 2020;10:4560 pubmed publisher
Gomez I, Ward B, Souilhol C, Recarti C, Ariaans M, Johnston J, et al. Neutrophil microvesicles drive atherosclerosis by delivering miR-155 to atheroprone endothelium. Nat Commun. 2020;11:214 pubmed publisher
Wood M, Wood L, Templeton M, Fisher B, Lippy A, Jones C, et al. Transient Immune Activation in BCG-Vaccinated Infant Rhesus Macaques Is Not Sufficient to Influence Oral Simian Immunodeficiency Virus Infection. J Infect Dis. 2020;222:44-53 pubmed publisher
Foote J, Patel A, Yona S, Segal A. Variations in the Phagosomal Environment of Human Neutrophils and Mononuclear Phagocyte Subsets. Front Immunol. 2019;10:188 pubmed publisher
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Lin H, Chen S, Guo J, Su I, Huang C, Chien C, et al. Dynamic expression of SMAD3 is critical in osteoblast differentiation of PDMCs. Int J Mol Med. 2019;43:1085-1093 pubmed publisher
Zimmerman M, Quicke K, O Neal J, Arora N, Machiah D, Priyamvada L, et al. Cross-Reactive Dengue Virus Antibodies Augment Zika Virus Infection of Human Placental Macrophages. Cell Host Microbe. 2018;24:731-742.e6 pubmed publisher
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Woodberry T, Loughland J, Minigo G, Burel J, Amante F, Piera K, et al. Early Immune Regulatory Changes in a Primary Controlled Human Plasmodium vivax Infection: CD1c+ Myeloid Dendritic Cell Maturation Arrest, Induction of the Kynurenine Pathway, and Regulatory T Cell Activation. Infect Immun. 2017;85: pubmed publisher
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Zizzo G, Hilliard B, Monestier M, Cohen P. Efficient clearance of early apoptotic cells by human macrophages requires M2c polarization and MerTK induction. J Immunol. 2012;189:3508-20 pubmed
product information
Antigen :
CD14
Apps. Abbrev. :
FC
Cat # :
301850
Clone :
M5E2
Item :
PE anti-human CD14
Isotype :
Mouse IgG2a, κ
Other Names :
LPS receptor
Size :
100 μg
Price (USD) :
146 USD
Reactivity :
Human, Cynomolgus, Rhesus
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
PE
Immunogen :
Full-length human CD14 protein
Application Notes :
The M5E2 antibody inhibits monocyte activation and cytokine production induced by LPS. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections, blocking of LPS stimulation^4, and immunofluorescence microscopy^5. Clone M5E2 is not recommended for immunohistochemical staining of formalin-fixed paraffin-embedded sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 301861 and 301862).;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA