product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
PerCP anti-human CD14
catalog :
301847
quantity :
25 tests
price :
136 USD
clonality :
monoclonal
host :
mouse
conjugate :
PerCP
clone name :
M5E2
reactivity :
human, rhesus macaque
application :
flow cytometry
more info or order :
citations: 11
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human
Bendall S, Simonds E, Qiu P, Amir E, Krutzik P, Finck R, et al. Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum. Science. 2011;332:687-96 pubmed publisher
Charvet B, Brunel J, Pierquin J, Iampietro M, Decimo D, Queruel N, et al. SARS-CoV-2 awakens ancient retroviral genes and the expression of proinflammatory HERV-W envelope protein in COVID-19 patients. iScience. 2023;26:106604 pubmed publisher
Weskamm L, Dahlke C, Addo M. Flow cytometric protocol to characterize human memory B cells directed against SARS-CoV-2 spike protein antigens. STAR Protoc. 2022;3:101902 pubmed publisher
Weskamm L, Fathi A, Raadsen M, Mykytyn A, Koch T, Spohn M, et al. Persistence of MERS-CoV-spike-specific B cells and antibodies after late third immunization with the MVA-MERS-S vaccine. Cell Rep Med. 2022;3:100685 pubmed publisher
Moorlag S, Rodriguez Rosales Y, Gillard J, Fanucchi S, Theunissen K, Novakovic B, et al. BCG Vaccination Induces Long-Term Functional Reprogramming of Human Neutrophils. Cell Rep. 2020;33:108387 pubmed publisher
Giamarellos Bourboulis E, Tsilika M, Moorlag S, Antonakos N, Kotsaki A, Domínguez Andrés J, et al. Activate: Randomized Clinical Trial of BCG Vaccination against Infection in the Elderly. Cell. 2020;183:315-323.e9 pubmed publisher
Morris A, Farley C, Pinelli D, Adams L, Cragg M, Boss J, et al. Signaling through the Inhibitory Fc Receptor FcγRIIB Induces CD8+ T Cell Apoptosis to Limit T Cell Immunity. Immunity. 2020;52:136-150.e6 pubmed publisher
Salvany Celades M, van der Zwan A, Benner M, Setrajcic Dragos V, Bougleux Gomes H, Iyer V, et al. Three Types of Functional Regulatory T Cells Control T Cell Responses at the Human Maternal-Fetal Interface. Cell Rep. 2019;27:2537-2547.e5 pubmed publisher
Zizzo G, Hilliard B, Monestier M, Cohen P. Efficient clearance of early apoptotic cells by human macrophages requires M2c polarization and MerTK induction. J Immunol. 2012;189:3508-20 pubmed
Santer D, Hall B, George T, Tangsombatvisit S, Liu C, Arkwright P, et al. C1q deficiency leads to the defective suppression of IFN-alpha in response to nucleoprotein containing immune complexes. J Immunol. 2010;185:4738-49 pubmed publisher
Iwamoto S, Iwai S, Tsujiyama K, Kurahashi C, Takeshita K, Naoe M, et al. TNF-alpha drives human CD14+ monocytes to differentiate into CD70+ dendritic cells evoking Th1 and Th17 responses. J Immunol. 2007;179:1449-57 pubmed
product information
Antigen :
CD14
Apps. Abbrev. :
FC
Cat # :
301847
Clone :
M5E2
Item :
PerCP anti-human CD14
Isotype :
Mouse IgG2a, κ
Other Names :
LPS receptor
Size :
25 tests
Price (USD) :
136 USD
Reactivity :
Human, Cynomolgus, Rhesus
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
PERCP
Immunogen :
Full-length human CD14 protein
Application Notes :
The M5E2 antibody inhibits monocyte activation and cytokine production induced by LPS. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections, blocking of LPS stimulation^4, and immunofluorescence microscopy^5. Clone M5E2 is not recommended for immunohistochemical staining of formalin-fixed paraffin-embedded sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 301861 and 301862).;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA