Ultra-LEAF™ Purified anti-human CD14 | BioLegend 301846 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

Ultra-LEAF™ Purified anti-human CD14

catalog :

301846

quantity :

500 μg

price :

365 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

M5E2

reactivity :

African green monkey, human, mouse, dog, cow, chimpanzee, Rhesus monkey

application :

neutralization, flow cytometry, immunohistochemistry - frozen section

citations: 26

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; 1:200; loading ...; fig 1b	Jones G, Bain C, Fenton T, Kelly A, Brown S, Ivens A, et al. Dynamics of Colon Monocyte and Macrophage Activation During Colitis. Front Immunol. 2018;9:2764 pubmed publisher
flow cytometry; human; loading ...; fig s1	Risnes L, Christophersen A, Dahal Koirala S, Neumann R, Sandve G, Sarna V, et al. Disease-driving CD4+ T cell clonotypes persist for decades in celiac disease. J Clin Invest. 2018;128:2642-2650 pubmed publisher
flow cytometry; human; loading ...; fig 2c	Clayton K, Collins D, Lengieza J, Ghebremichael M, Dotiwala F, Lieberman J, et al. Resistance of HIV-infected macrophages to CD8+ T lymphocyte-mediated killing drives activation of the immune system. Nat Immunol. 2018;19:475-486 pubmed publisher
flow cytometry; human; loading ...; fig s1	Melo Gonzalez F, Fenton T, Forss C, Smedley C, Goenka A, MacDonald A, et al. Intestinal mucin activates human dendritic cells and IL-8 production in a glycan-specific manner. J Biol Chem. 2018;293:8543-8553 pubmed publisher
flow cytometry; African green monkey; loading ...; fig 2a	Holbrook B, Aycock S, Machiele E, Clemens E, Gries D, Jorgensen M, et al. An R848 adjuvanted influenza vaccine promotes early activation of B cells in the draining lymph nodes of non-human primate neonates. Immunology. 2018;153:357-367 pubmed publisher
flow cytometry; human; loading ...; fig s4a	Salio M, Gasser O, González López C, Martens A, Veerapen N, Gileadi U, et al. Activation of Human Mucosal-Associated Invariant T Cells Induces CD40L-Dependent Maturation of Monocyte-Derived and Primary Dendritic Cells. J Immunol. 2017;199:2631-2638 pubmed publisher
flow cytometry; human; loading ...	Bergström I, Lundberg A, Jonsson S, Särndahl E, Ernerudh J, Jonasson L. Annexin A1 in blood mononuclear cells from patients with coronary artery disease: Its association with inflammatory status and glucocorticoid sensitivity. PLoS ONE. 2017;12:e0174177 pubmed publisher
flow cytometry; human; loading ...	Tauriainen J, Scharf L, Frederiksen J, Naji A, Ljunggren H, Sonnerborg A, et al. Perturbed CD8+ T cell TIGIT/CD226/PVR axis despite early initiation of antiretroviral treatment in HIV infected individuals. Sci Rep. 2017;7:40354 pubmed publisher
flow cytometry; mouse; loading ...; fig s4	Cheng L, Ma J, Li J, Li D, Li G, Li F, et al. Blocking type I interferon signaling enhances T cell recovery and reduces HIV-1 reservoirs. J Clin Invest. 2017;127:269-279 pubmed publisher
flow cytometry; Rhesus monkey; loading ...	Roberts E, Carnathan D, Li H, Shaw G, Silvestri G, Betts M. Collapse of Cytolytic Potential in SIV-Specific CD8+ T Cells Following Acute SIV Infection in Rhesus Macaques. PLoS Pathog. 2016;12:e1006135 pubmed publisher
flow cytometry; human; loading ...; fig s4	Andresen V, Erikstein B, Mukherjee H, Sulen A, Popa M, SÃ¸rnes S, et al. Anti-proliferative activity of the NPM1 interacting natural product avrainvillamide in acute myeloid leukemia. Cell Death Dis. 2016;7:e2497 pubmed publisher
flow cytometry; human; loading ...	Demers K, Makedonas G, Buggert M, Eller M, Ratcliffe S, Goonetilleke N, et al. Temporal Dynamics of CD8+ T Cell Effector Responses during Primary HIV Infection. PLoS Pathog. 2016;12:e1005805 pubmed publisher
flow cytometry; human; loading ...; fig 1a	Gadd V, Patel P, Jose S, Horsfall L, Powell E, Irvine K. Altered Peripheral Blood Monocyte Phenotype and Function in Chronic Liver Disease: Implications for Hepatic Recruitment and Systemic Inflammation. PLoS ONE. 2016;11:e0157771 pubmed publisher
flow cytometry; human; loading ...; fig 1b	Malkiel S, Jeganathan V, Wolfson S, Manjarrez Orduno N, Marasco E, Aranow C, et al. Checkpoints for Autoreactive B Cells in the Peripheral Blood of Lupus Patients Assessed by Flow Cytometry. Arthritis Rheumatol. 2016;68:2210-20 pubmed publisher
flow cytometry; human; fig 2	Offersen R, Nissen S, Rasmussen T, Ã˜stergaard L, Denton P, SÃ¸gaard O, et al. A Novel Toll-Like Receptor 9 Agonist, MGN1703, Enhances HIV-1 Transcription and NK Cell-Mediated Inhibition of HIV-1-Infected Autologous CD4+ T Cells. J Virol. 2016;90:4441-4453 pubmed publisher
flow cytometry; mouse	Amos J, Himes J, Armand L, Gurley T, Martinez D, Colvin L, et al. Rapid Development of gp120-Focused Neutralizing B Cell Responses during Acute Simian Immunodeficiency Virus Infection of African Green Monkeys. J Virol. 2015;89:9485-98 pubmed publisher
flow cytometry; human; fig s5	O Brien E, Abdulahad W, Rutgers A, Huitema M, O Reilly V, Coughlan A, et al. Intermediate monocytes in ANCA vasculitis: increased surface expression of ANCA autoantigens and IL-1Î² secretion in response to anti-MPO antibodies. Sci Rep. 2015;5:11888 pubmed publisher
flow cytometry; human; fig 1	Boyle M, Jagannathan P, Bowen K, McIntyre T, Vance H, Farrington L, et al. Effector Phenotype of Plasmodium falciparum-Specific CD4+ T Cells Is Influenced by Both Age and Transmission Intensity in Naturally Exposed Populations. J Infect Dis. 2015;212:416-25 pubmed publisher
flow cytometry; human	Heninger A, Wentrup S, Al Saeedi M, Schiessling S, Giese T, Wartha F, et al. Immunomodulation of human intestinal T cells by the synthetic CD80 antagonist RhuDex®. Immun Inflamm Dis. 2014;2:166-80 pubmed publisher
flow cytometry; Rhesus monkey	Li H, Evans T, Gillis J, Connole M, Reeves R. Bone marrow-imprinted gut-homing of plasmacytoid dendritic cells (pDCs) in acute simian immunodeficiency virus infection results in massive accumulation of hyperfunctional CD4+ pDCs in the mucosae. J Infect Dis. 2015;211:1717-25 pubmed publisher
flow cytometry; human	Tungatt K, Bianchi V, Crowther M, Powell W, Schauenburg A, Trimby A, et al. Antibody stabilization of peptide-MHC multimers reveals functional T cells bearing extremely low-affinity TCRs. J Immunol. 2015;194:463-74 pubmed publisher
flow cytometry; human; loading ...; fig 1	Duggal N, Beswetherick A, Upton J, Hampson P, Phillips A, Lord J. Depressive symptoms in hip fracture patients are associated with reduced monocyte superoxide production. Exp Gerontol. 2014;54:27-34 pubmed publisher
flow cytometry; human	Bendall S, Simonds E, Qiu P, Amir E, Krutzik P, Finck R, et al. Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum. Science. 2011;332:687-96 pubmed publisher
	Zizzo G, Hilliard B, Monestier M, Cohen P. Efficient clearance of early apoptotic cells by human macrophages requires M2c polarization and MerTK induction. J Immunol. 2012;189:3508-20 pubmed
	Santer D, Hall B, George T, Tangsombatvisit S, Liu C, Arkwright P, et al. C1q deficiency leads to the defective suppression of IFN-alpha in response to nucleoprotein containing immune complexes. J Immunol. 2010;185:4738-49 pubmed publisher
	Iwamoto S, Iwai S, Tsujiyama K, Kurahashi C, Takeshita K, Naoe M, et al. TNF-alpha drives human CD14+ monocytes to differentiate into CD70+ dendritic cells evoking Th1 and Th17 responses. J Immunol. 2007;179:1449-57 pubmed

product information

Antigen :

CD14

Apps. Abbrev. :

Flow Cytometry, CyTOF®, Block, IHC-F

Cat # :

301846

Clone :

M5E2

Item :

Ultra-LEAF™ Purified anti-human CD14

Isotype :

Mouse IgG2a, κ

Other Names :

LPS receptor

Size :

500 μg

Price (USD) :

365 USD

Reactivity :

Human, African Green, Capuchin Monkey, Cattle (Bovine, Cow), Chimpanzee, Common Marmoset, Cotton-topped Tamarin, Cynomolgus, Dog (Canine), Rhesus, Pigtailed Macaque, Squirrel Monkey

Clonality :

Monoclonal

Host :

Mouse

Conjugate/Tag/Label :

ULEAF

Immunogen :

Full-length human CD14 protein

Application Notes :

The M5E2 antibody inhibits monocyte activation and cytokine production induced by LPS. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections, blocking of LPS stimulation 4 , and immunofluorescence microscopy 5 . Clone M5E2 is not recommended for immunohistochemical staining of formalin-fixed paraffin-embedded sections. The LEAF™ purified antibody (Endotoxin 0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 301810).