product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Brilliant Violet 711™ anti-human CD14
catalog :
301837
quantity :
25 tests
price :
220 USD
clonality :
monoclonal
host :
mouse
conjugate :
BV711
clone name :
M5E2
reactivity :
human, rhesus macaque
application :
flow cytometry
more info or order :
citations: 23
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; loading ...; fig 3a
Allden S, Ogger P, Ghai P, McErlean P, Hewitt R, Toshner R, et al. The Transferrin Receptor CD71 Delineates Functionally Distinct Airway Macrophage Subsets during Idiopathic Pulmonary Fibrosis. Am J Respir Crit Care Med. 2019;: pubmed publisher
  • flow cytometry; human
Bendall S, Simonds E, Qiu P, Amir E, Krutzik P, Finck R, et al. Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum. Science. 2011;332:687-96 pubmed publisher
Houlder E, Costain A, Nambuya I, Brown S, Koopman J, Langenberg M, et al. Pulmonary inflammation promoted by type-2 dendritic cells is a feature of human and murine schistosomiasis. Nat Commun. 2023;14:1863 pubmed publisher
Gargaro M, Scalisi G, Manni G, Briseño C, Bagadia P, Durai V, et al. Indoleamine 2,3-dioxygenase 1 activation in mature cDC1 promotes tolerogenic education of inflammatory cDC2 via metabolic communication. Immunity. 2022;55:1032-1050.e14 pubmed publisher
Biswas S, Rust L, Wettengel J, Yusova S, Fischer M, Carson J, et al. Long-term hepatitis B virus infection of rhesus macaques requires suppression of host immunity. Nat Commun. 2022;13:2995 pubmed publisher
Mujal A, Combes A, Rao A, Binnewies M, Samad B, TSUI J, et al. Holistic Characterization of Tumor Monocyte-to-Macrophage Differentiation Integrates Distinct Immune Phenotypes in Kidney Cancer. Cancer Immunol Res. 2022;10:403-419 pubmed publisher
Vijayakumar B, Boustani K, Ogger P, Papadaki A, Tonkin J, Orton C, et al. Immuno-proteomic profiling reveals aberrant immune cell regulation in the airways of individuals with ongoing post-COVID-19 respiratory disease. Immunity. 2022;55:542-556.e5 pubmed publisher
Combes A, Samad B, TSUI J, Chew N, Yan P, Reeder G, et al. Discovering dominant tumor immune archetypes in a pan-cancer census. Cell. 2022;185:184-203.e19 pubmed publisher
Joyner C, Ley A, Nguyen D, Ali M, Corrado A, Tipton C, et al. Generation of human long-lived plasma cells by developmentally regulated epigenetic imprinting. Life Sci Alliance. 2022;5: pubmed publisher
Cossarizza A, Chang H, Radbruch A, Abrignani S, Addo R, Akdis M, et al. Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition). Eur J Immunol. 2021;51:2708-3145 pubmed publisher
Karim F, Gazy I, Cele S, Zungu Y, Krause R, Bernstein M, et al. HIV status alters disease severity and immune cell responses in Beta variant SARS-CoV-2 infection wave. elife. 2021;10: pubmed publisher
Platten M, Bunse L, Wick A, Bunse T, Le Cornet L, Harting I, et al. A vaccine targeting mutant IDH1 in newly diagnosed glioma. Nature. 2021;592:463-468 pubmed publisher
Robinson G, Waddington K, Coelewij L, Peng J, Naja M, Wincup C, et al. Increased apolipoprotein-B:A1 ratio predicts cardiometabolic risk in patients with juvenile onset SLE. EBioMedicine. 2021;65:103243 pubmed publisher
Combes A, Courau T, Kuhn N, Hu K, Ray A, Chen W, et al. Global absence and targeting of protective immune states in severe COVID-19. Nature. 2021;591:124-130 pubmed publisher
Esaulova E, Das S, Singh D, Choreño Parra J, Swain A, Arthur L, et al. The immune landscape in tuberculosis reveals populations linked to disease and latency. Cell Host Microbe. 2021;29:165-178.e8 pubmed publisher
Argüello R, Combes A, Char R, Gigan J, Baaziz A, Bousiquot E, et al. SCENITH: A Flow Cytometry-Based Method to Functionally Profile Energy Metabolism with Single-Cell Resolution. Cell Metab. 2020;32:1063-1075.e7 pubmed publisher
Kwok I, Becht E, Xia Y, Ng M, Teh Y, Tan L, et al. Combinatorial Single-Cell Analyses of Granulocyte-Monocyte Progenitor Heterogeneity Reveals an Early Uni-potent Neutrophil Progenitor. Immunity. 2020;: pubmed publisher
Cirovic B, de Bree L, Groh L, Blok B, Chan J, van der Velden W, et al. BCG Vaccination in Humans Elicits Trained Immunity via the Hematopoietic Progenitor Compartment. Cell Host Microbe. 2020;28:322-334.e5 pubmed publisher
Klichinsky M, Ruella M, Shestova O, Lu X, Best A, Zeeman M, et al. Human chimeric antigen receptor macrophages for cancer immunotherapy. Nat Biotechnol. 2020;38:947-953 pubmed publisher
Abd Hamid M, Wang R, Yao X, Fan P, Li X, Chang X, et al. Enriched HLA-E and CD94/NKG2A interaction limits antitumor CD8+ tumor-infiltrating T lymphocyte responses. Cancer Immunol Res. 2019;: pubmed publisher
Zizzo G, Hilliard B, Monestier M, Cohen P. Efficient clearance of early apoptotic cells by human macrophages requires M2c polarization and MerTK induction. J Immunol. 2012;189:3508-20 pubmed
Santer D, Hall B, George T, Tangsombatvisit S, Liu C, Arkwright P, et al. C1q deficiency leads to the defective suppression of IFN-alpha in response to nucleoprotein containing immune complexes. J Immunol. 2010;185:4738-49 pubmed publisher
Iwamoto S, Iwai S, Tsujiyama K, Kurahashi C, Takeshita K, Naoe M, et al. TNF-alpha drives human CD14+ monocytes to differentiate into CD70+ dendritic cells evoking Th1 and Th17 responses. J Immunol. 2007;179:1449-57 pubmed
product information
Antigen :
CD14
Apps. Abbrev. :
FC
Cat # :
301837
Clone :
M5E2
Item :
Brilliant Violet 711™ anti-human CD14
Isotype :
Mouse IgG2a, κ
Other Names :
LPS receptor
Size :
25 tests
Price (USD) :
220 USD
Reactivity :
Human, Cynomolgus, Rhesus
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
BV711
Immunogen :
Full-length human CD14 protein
Application Notes :
The M5E2 antibody inhibits monocyte activation and cytokine production induced by LPS. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections, blocking of LPS stimulation^4, and immunofluorescence microscopy^5. Clone M5E2 is not recommended for immunohistochemical staining of formalin-fixed paraffin-embedded sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 301861 and 301862).;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA