product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Brilliant Violet 605™ anti-human CD14
catalog :
301834
quantity :
100 tests
price :
396 USD
clonality :
monoclonal
host :
mouse
conjugate :
BV605
clone name :
M5E2
reactivity :
human, mouse, rhesus macaque
application :
flow cytometry
more info or order :
citations: 35
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; loading ...
Li D, Edwards R, Manne K, Martinez D, Schäfer A, Alam S, et al. In vitro and in vivo functions of SARS-CoV-2 infection-enhancing and neutralizing antibodies. Cell. 2021;184:4203-4219.e32 pubmed publisher
  • flow cytometry; human; loading ...; fig 3s2a
Gregorova M, Morse D, Brignoli T, Steventon J, Hamilton F, Albur M, et al. Post-acute COVID-19 associated with evidence of bystander T-cell activation and a recurring antibiotic-resistant bacterial pneumonia. elife. 2020;9: pubmed publisher
  • flow cytometry; human; loading ...; fig s4a
Salio M, Gasser O, González López C, Martens A, Veerapen N, Gileadi U, et al. Activation of Human Mucosal-Associated Invariant T Cells Induces CD40L-Dependent Maturation of Monocyte-Derived and Primary Dendritic Cells. J Immunol. 2017;199:2631-2638 pubmed publisher
  • flow cytometry; mouse
Amos J, Himes J, Armand L, Gurley T, Martinez D, Colvin L, et al. Rapid Development of gp120-Focused Neutralizing B Cell Responses during Acute Simian Immunodeficiency Virus Infection of African Green Monkeys. J Virol. 2015;89:9485-98 pubmed publisher
  • flow cytometry; human
Bendall S, Simonds E, Qiu P, Amir E, Krutzik P, Finck R, et al. Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum. Science. 2011;332:687-96 pubmed publisher
Schanin J, Korver W, Brock E, Leung J, Benet Z, Luu T, et al. Discovery of an agonistic Siglec-6 antibody that inhibits and reduces human mast cells. Commun Biol. 2022;5:1226 pubmed publisher
Weaver J, Stack E, Bugg xe9 J, Hu C, McGrath L, Mueller A, et al. Differential expression of CCR8 in tumors versus normal tissue allows specific depletion of tumor-infiltrating T regulatory cells by GS-1811, a novel Fc-optimized anti-CCR8 antibody. Oncoimmunology. 2022;11:2141007 pubmed publisher
Ronaldson Bouchard K, Teles D, Yeager K, Tavakol D, Zhao Y, Chramiec A, et al. A multi-organ chip with matured tissue niches linked by vascular flow. Nat Biomed Eng. 2022;6:351-371 pubmed publisher
Douthwaite H, Arteagabeitia A, Mukhopadhyay S. Differentiation of Human Induced Pluripotent Stem Cell into Macrophages. Bio Protoc. 2022;12:e4361 pubmed publisher
Wu X, Roberto J, Knupp A, Greninger A, Truong C, Hollingshead N, et al. Response of Human Liver Tissue to Innate Immune Stimuli. Front Immunol. 2022;13:811551 pubmed publisher
. A blood atlas of COVID-19 defines hallmarks of disease severity and specificity. Cell. 2022;185:916-938.e58 pubmed publisher
Kfoury Y, Baryawno N, Severe N, Mei S, Gustafsson K, Hirz T, et al. Human prostate cancer bone metastases have an actionable immunosuppressive microenvironment. Cancer Cell. 2021;39:1464-1478.e8 pubmed publisher
Riegel K, Yurugi H, Schlöder J, Jonuleit H, Kaulich M, Kirschner F, et al. ERK5 modulates IL-6 secretion and contributes to tumor-induced immune suppression. Cell Death Dis. 2021;12:969 pubmed publisher
Rice T, Diavatopoulos D, Guo Y, Donaldson B, Bouqueau M, Bosanquet A, et al. Modification of innate immune responses to Bordetella pertussis in babies from pertussis vaccinated pregnancies. EBioMedicine. 2021;72:103612 pubmed publisher
Wimmers F, Donato M, Kuo A, Ashuach T, Gupta S, Li C, et al. The single-cell epigenomic and transcriptional landscape of immunity to influenza vaccination. Cell. 2021;184:3915-3935.e21 pubmed publisher
Vora A, Mondala P, Costello C, MacLeod A, Crews L. Sensitive intranuclear flow cytometric quantification of IRF4 protein in multiple myeloma and normal human hematopoietic cells. STAR Protoc. 2021;2:100565 pubmed publisher
L xe9 vy Y, Wiedemann A, Hejblum B, Durand M, Lefebvre C, Sur xe9 naud M, et al. CD177, a specific marker of neutrophil activation, is associated with coronavirus disease 2019 severity and death. iScience. 2021;24:102711 pubmed publisher
Thompson E, Cascino K, Ordonez A, Zhou W, Vaghasia A, Hamacher Brady A, et al. Metabolic programs define dysfunctional immune responses in severe COVID-19 patients. Cell Rep. 2021;34:108863 pubmed publisher
Mondala P, Vora A, Zhou T, Lazzari E, Ladel L, Luo X, et al. Selective antisense oligonucleotide inhibition of human IRF4 prevents malignant myeloma regeneration via cell cycle disruption. Cell Stem Cell. 2021;28:623-636.e9 pubmed publisher
Alsaleh G, Panse I, Swadling L, Zhang H, Richter F, Meyer A, et al. Autophagy in T cells from aged donors is maintained by spermidine and correlates with function and vaccine responses. elife. 2020;9: pubmed publisher
Wang J, Xu Y, Chen Z, Liang J, Lin Z, Liang H, et al. Liver Immune Profiling Reveals Pathogenesis and Therapeutics for Biliary Atresia. Cell. 2020;183:1867-1883.e26 pubmed publisher
Wiedemann A, Foucat E, Hocini H, Lefebvre C, Hejblum B, Durand M, et al. Long-lasting severe immune dysfunction in Ebola virus disease survivors. Nat Commun. 2020;11:3730 pubmed publisher
PLUM T, Wang X, Rettel M, Krijgsveld J, Feyerabend T, Rodewald H. Human Mast Cell Proteome Reveals Unique Lineage, Putative Functions, and Structural Basis for Cell Ablation. Immunity. 2020;52:404-416.e5 pubmed publisher
D Antoni M, Kallianpur K, Premeaux T, Corley M, Fujita T, Laws E, et al. Lower Interferon Regulatory Factor-8 Expression in Peripheral Myeloid Cells Tracks With Adverse Central Nervous System Outcomes in Treated HIV Infection. Front Immunol. 2019;10:2789 pubmed publisher
Brown C, Gudjonson H, Pritykin Y, Deep D, Lavallée V, Mendoza A, et al. Transcriptional Basis of Mouse and Human Dendritic Cell Heterogeneity. Cell. 2019;179:846-863.e24 pubmed publisher
Hagan T, Cortese M, Rouphael N, Boudreau C, Linde C, Maddur M, et al. Antibiotics-Driven Gut Microbiome Perturbation Alters Immunity to Vaccines in Humans. Cell. 2019;178:1313-1328.e13 pubmed publisher
Krebs S, Kwon Y, Schramm C, Law W, Donofrio G, Zhou K, et al. Longitudinal Analysis Reveals Early Development of Three MPER-Directed Neutralizing Antibody Lineages from an HIV-1-Infected Individual. Immunity. 2019;50:677-691.e13 pubmed publisher
Motwani M, Colas R, George M, Flint J, Dalli J, Richard Loendt A, et al. Pro-resolving mediators promote resolution in a human skin model of UV-killed Escherichia coli-driven acute inflammation. JCI Insight. 2018;3: pubmed publisher
Wu X, Roberto J, Knupp A, Kenerson H, Truong C, Yuen S, et al. Precision-cut human liver slice cultures as an immunological platform. J Immunol Methods. 2018;455:71-79 pubmed publisher
Böiers C, Richardson S, Laycock E, Zriwil A, Turati V, Brown J, et al. A Human IPS Model Implicates Embryonic B-Myeloid Fate Restriction as Developmental Susceptibility to B Acute Lymphoblastic Leukemia-Associated ETV6-RUNX1. Dev Cell. 2018;44:362-377.e7 pubmed publisher
Cale E, Gorman J, Radakovich N, Crooks E, Osawa K, Tong T, et al. Virus-like Particles Identify an HIV V1V2 Apex-Binding Neutralizing Antibody that Lacks a Protruding Loop. Immunity. 2017;46:777-791.e10 pubmed publisher
Takeshita Y, Obermeier B, Cotleur A, Spampinato S, Shimizu F, Yamamoto E, et al. Effects of neuromyelitis optica-IgG at the blood-brain barrier in vitro. Neurol Neuroimmunol Neuroinflamm. 2017;4:e311 pubmed publisher
Schaper F, de Leeuw K, Horst G, Bootsma H, Limburg P, Heeringa P, et al. High mobility group box 1 skews macrophage polarization and negatively influences phagocytosis of apoptotic cells. Rheumatology (Oxford). 2016;55:2260-2270 pubmed
Aschbacher K, Milush J, Gilbert A, Almeida C, Sinclair E, Epling L, et al. Chronic stress is associated with reduced circulating hematopoietic progenitor cell number: A maternal caregiving model. Brain Behav Immun. 2017;59:245-252 pubmed publisher
Zizzo G, Hilliard B, Monestier M, Cohen P. Efficient clearance of early apoptotic cells by human macrophages requires M2c polarization and MerTK induction. J Immunol. 2012;189:3508-20 pubmed
product information
Antigen :
CD14
Apps. Abbrev. :
FC
Cat # :
301834
Clone :
M5E2
Item :
Brilliant Violet 605™ anti-human CD14
Isotype :
Mouse IgG2a, κ
Other Names :
LPS receptor
Size :
100 tests
Price (USD) :
396 USD
Reactivity :
Human, Cynomolgus, Rhesus
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
BV605
Immunogen :
Full-length human CD14 protein
Application Notes :
The M5E2 antibody inhibits monocyte activation and cytokine production induced by LPS. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections, blocking of LPS stimulation^4, and immunofluorescence microscopy^5. Clone M5E2 is not recommended for immunohistochemical staining of formalin-fixed paraffin-embedded sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 301861 and 301862).;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA