product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Brilliant Violet 421™ anti-human CD14
catalog :
301830
quantity :
100 tests
price :
402 USD
clonality :
monoclonal
host :
mouse
conjugate :
BV421
clone name :
M5E2
reactivity :
human, rhesus macaque
application :
flow cytometry
more info or order :
citations: 34
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; loading ...; fig 1d
Pinkert J, Boehm H, Trautwein M, Doecke W, Wessel F, Ge Y, et al. T cell-mediated elimination of cancer cells by blocking CEACAM6-CEACAM1 interaction. Oncoimmunology. 2022;11:2008110 pubmed publisher
  • flow cytometry; human; loading ...; fig 1a
Gadd V, Patel P, Jose S, Horsfall L, Powell E, Irvine K. Altered Peripheral Blood Monocyte Phenotype and Function in Chronic Liver Disease: Implications for Hepatic Recruitment and Systemic Inflammation. PLoS ONE. 2016;11:e0157771 pubmed publisher
  • flow cytometry; human
Bendall S, Simonds E, Qiu P, Amir E, Krutzik P, Finck R, et al. Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum. Science. 2011;332:687-96 pubmed publisher
Wali G, Siow S, Liyanage E, Kumar K, Mackay Sim A, Sue C. Reduced acetylated α-tubulin in SPAST hereditary spastic paraplegia patient PBMCs. Front Neurosci. 2023;17:1073516 pubmed publisher
Porbahaie M, Savelkoul H, de Haan C, Teodorowicz M, van Neerven R. Direct Binding of Bovine IgG-Containing Immune Complexes to Human Monocytes and Their Putative Role in Innate Immune Training. Nutrients. 2022;14: pubmed publisher
Sugrue J, Posseme C, Tan Z, Pou C, Charbit B, Bondet V, et al. Enhanced TLR3 responsiveness in hepatitis C virus resistant women from the Irish anti-D cohort. Cell Rep Med. 2022;3:100804 pubmed publisher
Ronaghan N, Soo M, Pena U, Tellis M, Duan W, Tabatabaei Zavareh N, et al. M1-like, but not M0- or M2-like, macrophages, reduce RSV infection of primary bronchial epithelial cells in a media-dependent fashion. PLoS ONE. 2022;17:e0276013 pubmed publisher
Baharlou H, Canete N, Vine E, Hu K, Yuan D, Sandgren K, et al. An in situ analysis pipeline for initial host-pathogen interactions reveals signatures of human colorectal HIV transmission. Cell Rep. 2022;40:111385 pubmed publisher
Lende S, Pahus M, Monrad I, Olesen R, Mahr A, Vibholm L, et al. CD169 (Siglec-1) as a Robust Human Cell Biomarker of Toll-Like Receptor 9 Agonist Immunotherapy. Front Cell Infect Microbiol. 2022;12:919097 pubmed publisher
Maheshwari D, Saini K, Singh P, Singla M, Nayak K, Aggarwal C, et al. Contrasting behavior between the three human monocyte subsets in dengue pathophysiology. iScience. 2022;25:104384 pubmed publisher
Buters T, Hameeteman P, Jansen I, van Hindevoort F, Ten Voorde W, Florencia E, et al. Intradermal lipopolysaccharide challenge as an acute in vivo inflammatory model in healthy volunteers. Br J Clin Pharmacol. 2022;88:680-690 pubmed publisher
van der Wijst M, Vazquez S, Hartoularos G, Bastard P, Grant T, Bueno R, et al. Type I interferon autoantibodies are associated with systemic immune alterations in patients with COVID-19. Sci Transl Med. 2021;13:eabh2624 pubmed publisher
Sabree S, Voigt A, Blackwell S, Vishwakarma A, Chimenti M, Salem A, et al. Direct and indirect immune effects of CMP-001, a virus-like particle containing a TLR9 agonist. J Immunother Cancer. 2021;9: pubmed publisher
Cui D, Franz A, Fillon S, Jannetti L, Isambert T, Fundel Clemens K, et al. High-Yield Human Induced Pluripotent Stem Cell-Derived Monocytes and Macrophages Are Functionally Comparable With Primary Cells. Front Cell Dev Biol. 2021;9:656867 pubmed publisher
Vierboom M, Dijkman K, Sombroek C, Hofman S, Boot C, Vervenne R, et al. Stronger induction of trained immunity by mucosal BCG or MTBVAC vaccination compared to standard intradermal vaccination. Cell Rep Med. 2021;2:100185 pubmed publisher
Evren E, Ringqvist E, Tripathi K, Sleiers N, Rives I, Alisjahbana A, et al. Distinct developmental pathways from blood monocytes generate human lung macrophage diversity. Immunity. 2021;54:259-275.e7 pubmed publisher
Silvin A, Chapuis N, Dunsmore G, Goubet A, Dubuisson A, Derosa L, et al. Elevated Calprotectin and Abnormal Myeloid Cell Subsets Discriminate Severe from Mild COVID-19. Cell. 2020;: pubmed publisher
Laing A, Lorenc A, Del Molino Del Barrio I, Das A, Fish M, Monin L, et al. A dynamic COVID-19 immune signature includes associations with poor prognosis. Nat Med. 2020;26:1623-1635 pubmed publisher
Cheng C, Duan H, Xu K, Chuang G, Corrigan A, Geng H, et al. Immune Monitoring Reveals Fusion Peptide Priming to Imprint Cross-Clade HIV-Neutralizing Responses with a Characteristic Early B Cell Signature. Cell Rep. 2020;32:107981 pubmed publisher
Teijeira A, Garasa S, Gato M, Alfaro C, Miguéliz I, Cirella A, et al. CXCR1 and CXCR2 Chemokine Receptor Agonists Produced by Tumors Induce Neutrophil Extracellular Traps that Interfere with Immune Cytotoxicity. Immunity. 2020;52:856-871.e8 pubmed publisher
Juul Madsen K, Qvist P, Bendtsen K, Langkilde A, Vestergaard B, Howard K, et al. Size-Selective Phagocytic Clearance of Fibrillar α-Synuclein through Conformational Activation of Complement Receptor 4. J Immunol. 2020;204:1345-1361 pubmed publisher
Kong R, Duan H, Sheng Z, Xu K, Acharya P, Chen X, et al. Antibody Lineages with Vaccine-Induced Antigen-Binding Hotspots Develop Broad HIV Neutralization. Cell. 2019;178:567-584.e19 pubmed publisher
Tomellini E, Fares I, Lehnertz B, Chagraoui J, Mayotte N, Macrae T, et al. Integrin-α3 Is a Functional Marker of Ex Vivo Expanded Human Long-Term Hematopoietic Stem Cells. Cell Rep. 2019;28:1063-1073.e5 pubmed publisher
Willingham S, Ho P, Hotson A, Hill C, PICCIONE E, Hsieh J, et al. A2AR Antagonism with CPI-444 Induces Antitumor Responses and Augments Efficacy to Anti-PD-(L)1 and Anti-CTLA-4 in Preclinical Models. Cancer Immunol Res. 2018;6:1136-1149 pubmed publisher
Wu Q, Miao T, Feng T, Yang C, Guo Y, Li H. Dextran‑coated superparamagnetic iron oxide nanoparticles activate the MAPK pathway in human primary monocyte cells. Mol Med Rep. 2018;18:564-570 pubmed publisher
Haynes A, Linden M, Robey E, Naylor L, Ainslie P, Cox K, et al. Beneficial impacts of regular exercise on platelet function in sedentary older adults: evidence from a randomized 6-mo walking trial. J Appl Physiol (1985). 2018;125:401-408 pubmed publisher
Li C, Lev S, Desmarini D, Kaufman Francis K, Saiardi A, Silva A, et al. IP3-4 kinase Arg1 regulates cell wall homeostasis and surface architecture to promote clearance of Cryptococcus neoformans infection in a mouse model. Virulence. 2017;: pubmed publisher
Tyler C, McCarthy N, Lindsay J, Stagg A, Moser B, Eberl M. Antigen-Presenting Human γδ T Cells Promote Intestinal CD4+ T Cell Expression of IL-22 and Mucosal Release of Calprotectin. J Immunol. 2017;198:3417-3425 pubmed publisher
Haynes A, Linden M, Robey E, Watts G, BARRETT H, Naylor L, et al. Impact of commonly prescribed exercise interventions on platelet activation in physically inactive and overweight men. Physiol Rep. 2016;4: pubmed
Norton S, Dunn E, McCall J, Munro F, Kemp R. Gut macrophage phenotype is dependent on the tumor microenvironment in colorectal cancer. Clin Transl Immunology. 2016;5:e76 pubmed publisher
Rainho J, Martins M, Cunyat F, Watkins I, Watkins D, Stevenson M. Nef Is Dispensable for Resistance of Simian Immunodeficiency Virus-Infected Macrophages to CD8+ T Cell Killing. J Virol. 2015;89:10625-36 pubmed publisher
Zizzo G, Hilliard B, Monestier M, Cohen P. Efficient clearance of early apoptotic cells by human macrophages requires M2c polarization and MerTK induction. J Immunol. 2012;189:3508-20 pubmed
Santer D, Hall B, George T, Tangsombatvisit S, Liu C, Arkwright P, et al. C1q deficiency leads to the defective suppression of IFN-alpha in response to nucleoprotein containing immune complexes. J Immunol. 2010;185:4738-49 pubmed publisher
Iwamoto S, Iwai S, Tsujiyama K, Kurahashi C, Takeshita K, Naoe M, et al. TNF-alpha drives human CD14+ monocytes to differentiate into CD70+ dendritic cells evoking Th1 and Th17 responses. J Immunol. 2007;179:1449-57 pubmed
product information
Antigen :
CD14
Apps. Abbrev. :
FC
Cat # :
301830
Clone :
M5E2
Item :
Brilliant Violet 421™ anti-human CD14
Isotype :
Mouse IgG2a, κ
Other Names :
LPS receptor
Size :
100 tests
Price (USD) :
402 USD
Reactivity :
Human, Cynomolgus, Rhesus
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
BV421
Immunogen :
Full-length human CD14 protein
Application Notes :
The M5E2 antibody inhibits monocyte activation and cytokine production induced by LPS. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections, blocking of LPS stimulation^4, and immunofluorescence microscopy^5. Clone M5E2 is not recommended for immunohistochemical staining of formalin-fixed paraffin-embedded sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 301861 and 301862).;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA